ABSTRACT
BACKGROUND: SureFil SDR as a lining material has better adaptability and unique automatic leveling characteristics as compared with other flowing resins, but its application in preventive resin filling has not been reported. OBJECTIVE: To compare the roughness and bacterial adhesion among pit and fissure sealant, Z350 XT fluid resin and SureFil SDR resin, and to explore the feasibility of preventive resin filling with SureFil SDR resin. METHODS: Pit and fissure sealant, Z350 XT fluid resin and SureFil SDR resin were prepared into 8 mm× 2 mm× 2 mm cuboid specimens respectively. The surface roughness of the three specimens was measured by MarSurf roughness meter. Pit and fissure sealant, Z350 XT fluid resin and SureFil SDR resin were prepared into 8 mm× 8 mm× 2 mm cylinder specimens respectively. The specimens were cultured in Streptococcus mutans suspension for 48 hours to detect the bacterial adhesion on the material surface. RESULTS AND CONCLUSION: The roughness of the three materials was ranked as follows: SureFil SDR resin < Z350 XT fluid resin < pit and fissure sealant, and there were significant differences between groups (P < 0.05). The bacterial adhesion on the surface of the three materials was ranked as follows: SureFil SDR resin < Z350 XT fluid resin < pit and fissure sealant, and there were significant difference between groups (P < 0.05). The correlation between roughness and bacterial adhesion was analyzed, and there was a high positive correlation between roughness and bacterial adhesion, indicating, with the decrease of material roughness, bacterial adhesion decreased (r=0.962, P=0.017 6). The results show that SureFil SDR is an ideal preventive resin filling and sealing material relative to pit and fissure sealant and Z350 XT fluid resin.
ABSTRACT
This study was directed to the effects of macrophage-colony stimulating factors (M-CSF) concentration, recerptor activator of nuclear factor kappaB ligand (RANKL) concentration and M-CSF preinduction on osteoclastogenesis and the related resorption function. Bone marrow mononuclear cells were isolated and were divided into 4 groups. Group A underwent osteoclastogenic induction with the use of 30 ng/ml M-CSF and 50 ng/ml RANKL, while Group B received 50 ng/ml M-CSF and 100 ng/ml RANKL treatment. Both C and D Group underwent preinduction with the use of 30 ng/ml M-CSF for 3days, and then they were treated with 30 ng/ml M-CSF and 50 ng/ml RANKL, 50 ng/ml M-CSF and 100 ng/ml RANKL, respectively. Osteoclastogenesis was examined by TRAP staining 6 days after induction, and dentin resorption lacunae were detected by Scanning Electron Microscope 9 days after induction. TRAP positive multinuclear cells were observed in all groups of cells, and resorption lacunae were formed in all of them. However, more TRAP positive multinuclear cells were observed and more large resorption lacunae were detected in groups B and D than in groups A and C, respectively. The number of TRAP positive cells, number of resorption lacunae and lacuna areas in groups C and D were also greater than those in groups A and B, respectively. Higher concentration of M-CSF and RANKL and preinduction with M-CSF may benefit osteoclastogenesis and increase resorption function of osteoclast.
Subject(s)
Animals , Mice , Bone Marrow Cells , Cell Biology , Cell Culture Techniques , Methods , Cell Differentiation , Cell Proliferation , Cells, Cultured , Culture Media , Pharmacology , Leukocytes, Mononuclear , Cell Biology , Macrophage Colony-Stimulating Factor , Pharmacology , Osteoclasts , Cell Biology , RANK Ligand , PharmacologyABSTRACT
AIM: To study the effects of astragalosides (AS) on the proliferation and cell cycle of rat glomerular mesangial cell (MC), and verify the correlation between the influence and the AS concentration. METHODS: The experiment was carried out in the Physiological Laboratory of Liaoning Medical University from December 2006 to July 2007. Rat glomerular MCs were cultured in high glucose for 4-7 passages. The experiments were randomly divided into control group and three AS groups with different concentrations (50, 100, 200 mg/L), which were treated with high-glucose liquid and AS respectively. MC proliferation was determined with MTT colorimetric method in each group at hours 48 after intervention. MC cell cycle was detected with flow cytometry. RESULTS: ①MTT results showed that, the values of A490 nm in AS groups were lower than that in the control group at hours 48 (P