ABSTRACT
In January 2023,the European Society of Cardiology(ESC)Working Group on thrombosis published its 2022 updated consensus document on acute,perioperative,and long-term antithrombotic therapy for the elderly.Since the elderly are often accompanied with multiple organ changes and multiple diseases,the risk of hemorrhagic and ischemic events is increased,and they often take multiple drugs and have poor compliance with treatment,which pose significant challenges to clinical antithrombotic management.This article elaborates on how to assess the risk of thrombosis and bleeding,the treatment strategy of oral antithrombotic drugs,the treatment strategy of parenteral antithrombotic drugs,and the perioperative antithrombotic therapy protocols,with the aim of providing clinicians with references for the treatment of antithrombosis in the elderly.
ABSTRACT
OBJECTIVE@#To construct the co-expressing lentivirus vector of human glucocorticoid receptor (GR) beta and green fluorescent protein (GFP).@*METHOD@#cDNA encoding hGRbeta obtained from the expression library of fetal brain using gene recombinant technology was cloned into pGC-LV by double digests technology. The constructed lentivirus vector of pGC-FU-GRbeta that was confirmed the sequencing was correct, transfected 293T cells through lipofectamine 2000. The constructed lentivirus vector was identified by fluorescence detection and Western Blot method. The packed lentivirus vector was used to infect 293T cells. The titer of virus was tested by real-time quantitative PCR.@*RESULT@#A recombinant lentivirus vector co-expressing hGRbeta and GFP gene was constructed successfully. After transfection, a large number of 293T cells with green fluorescence were observed under fluorescent microscope, and the expression of GRbeta and GFP fusion protein was detected by Western Blot. The virus titer was 2.00E+8 TU/ml tested by Real-time PCR.@*CONCLUSION@#Successful construction of hGRbeta and GFP co-expressing lentivirus vector provides a stable vector for investigating the relationship between GRbeta and hormonal sensitivity or resistance in the therapy of chronic rhinosinusitis whose choice drug are glucocorticoids.