ABSTRACT
Objective To observe the correlationship of serum leptin,adiponectin,Th1/Th2 cytokine and symptom severity in obese adult asthma patients,and investigate the pathogenesis of obese adult asthma.Methods From July to December in 2016,100 patients with asthma in the People's Hospital of Zhuhai,and 50 cases of healthy physical examination in the same period were studied.According to body mass,50 cases were divided into obese asthma group (A group),normal body mass asthma group(B group),obesity physical examination group (C group) and normal body mass physical examination group (D group),with 25 cases in each group.The serum leptin,adiponectin,INF-γand IL-4 were detected using ELISA,as well as did the concurrent pulmonary function and ACT.The correlationship of serum leptin and adiponectin,INF-γ,IL-4,pulmonary function and ACT were evaluated.Results The serum leptin,adiponectin,IFN-γ,IL-4 and IFN-γ/IL-4 in A group were (9.67 ±2.86) μg/L,(8.03 ± 3.69) mg/L,(83.69 ± 25.11) ng/L,(22.40 ± 10.92) ng/L,(4.67 ± 2.63),respectively,which in B group were (5.98 ± 3.03) μg/L,(12.30 ± 5.41) mg/L,(41.88 ± 24.25) ng/L,(7.10 ± 7.83) ng/L,(1.20 ± 0.83),respectively,which in C group were (7.02 ± 3.36) μμg/L,(11.52 ± 4.52) mg/L,(37.49 ±25.33) ng/L,(21.25 ± 9.65) ng/L,(2.00 ± 1.13),respectively,which in D group were (4.55 ± 2.33) μg/L,(15.13 ± 5.28) mg/L,(34.37 ± 12.46) ng/L,(21.25 ± 9.65) ng/L,(1.87 ± 1.21),respectively,the differences among the four group were statistically significant(F =19.435,13.114,33.252,23.250,31.750,all P ＜ 0.05);The leptin levels showed a negative correlation with ACT and FEV1 ％ Pred(r =-0.501,-0.384,all P ＜ 0.05),but a positive correlation with IFN-γand IFN-γ/IL-4(r =0.446,0.423,all P ＜ 0.05),and no correlation with IL-4 (r =-0.189,P ＞ 0.05).The serum adiponectin in A group was lower than that in B group,C group and D group (Z =3.207,t =3.532,5.520,all P ＜ 0.05),and showed a positive correlation with ACT and FEV1 ％ Pred (r =0.361,0.466,all P ＜ 0.05);and showed no correlation with INF-γ,IL-4 and IFN-γ/IL-4 (r =-0.155,0.108,-0.161,all P ＞ 0.05).Conclusion The serum leptin in obese asthma patients has a higher levels and a positive correlation with clinical severity,and probably play a role in airway hyperresponsiveness by Th1 cytokine mediating.The serum adiponectin has a negative correlation with clinical severity,but no correlation with Th1/Th2 cytokine.
ABSTRACT
Objective To analyze the association between β2-AK 27 locus genetic polymorphisms and asthma, and the protective effect in airway hyperreactivity. Methods The allel polymerase chain reaction were used to determine β2-AR 27 locus genetic polymorphisms in 149 patients with cough variant asthma who have the airway hyperreactivity. To observe these people for two years in order to know the proportion of changed to typical asthma. And compare with 90 people in healthy group. Results (1) The distribution frequency of β2-AR 27 locus genetic polymorphisms is major in heterozygote (57 % ) , and the Glu/Glu homozygote has the least ( 20% ) , (2) There was a significant decrease in the frequency of Glu/Glu genotype in asthmatics compared with healthy group(9% VS 20% ) ,OR = 0.4(P<0.05) ,95% CI (0.2 ～0. 9) ,but there was no significant difference in the allele frequency of asthmatics compared with healthy group,(3)The frequency of Glu/Glu genotype in severe asthma was lower than stable asthma group(P<0. 05). Conclusion These results suggesteded that β2-AR 27 locus genetic polymorphisms is correlated with asthma,and the Glu27 could have the protective effect to the airway.
ABSTRACT
Objective To study pre-thrombosis state in patients with cor pulmonale and analyze the value of treatment with low molecular heparin (LMH). Method 46 patients with cor pulmonale were divided into LMH group and routine one. Von Willebrand factor (vWF), prethrombin F 1+2 fragment (F 1+2 ), Fibrinogen (Fg), granule membrane protein (GMP-140) and D-dimer fragment (DD) were detected separately.Result The concentrations of WF,F 1+2 ,Fg,GMP-140 and DD in the patients with cor pulmonale were significantly higher than control. In LMH group, these variables and PaCO 2 decreased and PaO 2 increased markedly after treatment. In routine group, these blood-gas index slightly improved, but no decrease were observed in coagulation variables. Conclusion Early detection and diagnosis of pre-thrombosis state in patients with cor polmanate and timely treatment with LMH can be beneficial to these patients during acute state.