ABSTRACT
<p><b>OBJECTIVE</b>To investigate the expression of neural salient serine/arginine-rich protein 1 (NSSR1) in the development of mouse brain.</p><p><b>METHODS</b>Brain samples were collected from mice with different developmental stages: 9, 12, 14 d before birth (E9, E12, E14) and 1 d, 3 weeks and 3 months after birth. The expression of NSSR1 in mouse brain at different developmental stages was detected by Western blot and the distribution of NSSR1 was analyzed by immunohistochemical staining. The expression and distribution of NSSR1 in mouse brain were compared among embryos, neonatal and adult animals.</p><p><b>RESULTS</b>During embryogenesis, the expression of NSSR1 proteins increases significantly from 0.186(E9) to 0.445(E14) and reached a high level after birth. Immunohistochemical analysis showed that in E12 embryos, NSSR1 was specifically distributed in the marginal and mantle layers. The expression of NSSR1 in hippocampus was very low in neonatal animals but stronger in adults. In cerebellar cortex, NSSR1 was widely expressed in purkinje and granule cells of adult animals, but mainly expressed in Purkinje cells in neonates.</p><p><b>CONCLUSION</b>The expression of NSSR1 is regulated by the development of mouse brain and presents dynamic changes.</p>
Subject(s)
Animals , Mice , Brain , Embryology , Metabolism , Cell Cycle Proteins , Metabolism , Neoplasm Proteins , Metabolism , RNA-Binding Proteins , Metabolism , Repressor Proteins , MetabolismABSTRACT
<p><b>OBJECTIVE</b>To establish a minigene model of neural cell adhesion molecule L1 (NCAM L1) gene and to study its splicing patterns in different cell lines.</p><p><b>METHODS</b>Using human genetic cDNA as template, the NCAM L1 minigene fragment was amplified and inserted into eukaryotic expression vector. The minigene was transfected into 4 cell lines and the splicing patterns of NCAM L1 minigene in these cell lines were studied.</p><p><b>RESULTS</b>The splicing patterns of NCAM L1 minigene were different in individual cell lines. In PFSK and Hela cell lines, two splicied isoforms were generated but in COS-1 and R28 cell lines, only one isoform existed.</p><p><b>CONCLUSION</b>NCAM L1 minigene model can be used in alternative splicing analysis.</p>