Expression of quorum-sensing related genes during Enterococcus faecalis biofilm formation / 中华口腔医学杂志

<p><b>OBJECTIVE</b>To investigate the relationship between the expression of the quorum-sensing related genes during Enterococcus faecalis(Ef) biofilm formation.</p><p><b>METHODS</b>Ef biofilms model was established in vitro and film formation process was observed by confocal laser scanning microscope at 6, 12, 24 and 48 hours respectively.Quantification of biofilms was achieved by staining with crystal violet.Real-time fluorescence quantitative PCR method was used to detect the expression of fsrB, gelE and sprE genes in the process of Ef biofilm formation.</p><p><b>RESULTS</b>A lot of live and dead bacteria unevenly distributed in Ef biofilm. The quantity of biofilms increased with time within 24 hours and was 0 h:0.00 ± 0.00, 6 h:1.09 ± 0.13, 12 h:2.10 ± 0.79, 24 h:3.30 ± 0.13, which was significantly different among the 4 time period(P < 0.05). The quantity of biofilm at 48 h(3.51 ± 0.01) increased slightly compared with 24 h(3.30 ± 0.13) , but did not show significant difference.Quantitative real-time PCR showed that the expression of quorum-sensing related fsrB increased with time within 24 hours and was 0 h:9.98 ± 0.46, 6 h:23.45 ± 1.13, 12 h:47.30 ± 2.49, 24 h: 331.30 ± 2.18, which was significantly different among the 4 time period(P < 0.05). The expression of gelE was 0 h: 6.54 ± 0.73, 6 h: 14.26 ± 1.24, 12 h: 37.47 ± 2.35, 24 h:264.80 ± 5.10(P < 0.05). The expression of sprE was 0 h: 7.72 ± 0.74, 6 h: 21.15 ± 0.96, 12 h:49.87 ± 3.18, 24 h:441.89 ± 7.74, which was significantly different among the 4 time period(P < 0.05).</p><p><b>CONCLUSIONS</b>The fsrB, gelE and sprE genes are closely related to the biofilm formation in Ef.</p>

Clinical study of immediate placement and early loading of dental implants / 中华口腔医学杂志

<p><b>OBJECTIVE</b>To evaluate the clinical results of immediate placement and early loading of dental implants.</p><p><b>METHODS</b>Sixty-four dental implants were inserted into the edentulous section of 37 patients immediately after the teeth were extracted. 36 implants were inserted by one-stage operation procedure, of which 17 implants were loaded immediately in 3 edentulous jaws by temporary mandibular overdenture. Fixed crown restoration were completed on 14 implants in 1 - 2 months and on 22 implants in 3 - 4 months. The two-stage operation for another 28 implants were performed after 3 - 6 months and then were restored by routine methods.</p><p><b>RESULTS</b>All implants were inserted successfully and stable. There was no peri-implantitis and X-ray films indicated that no remarkable bone resorption occurred. There were no significant differences in the peri-implant depths of gingival pocket between early loading implants and routine restoration.</p><p><b>CONCLUSIONS</b>Dental immediate implant and early loading after implantation can shorten the treatment period of implant-supported prosthesis. The short-term clinical performance was not different from the routine method.</p>