ABSTRACT
The present study carried out a phytochemical investigation of the methanol extract of the branches and leaves of Clausena lansium and afforded nine carbazole alkaloids (compounds 1-9) including two new carbazole alkaloids, claulansiums A and B (compounds 1 and 2). The new compounds were elucidated on the basis of extensive spectroscopic data (MS, NMR, IR, and UV) and the known compounds were identified by comparing spectroscopic data with those reported in literature. All the isolated compounds were tested for their cytotoxic activity against A549 and Hela cancer cell lines. Our results showed that compounds 2-6 exhibited varying degrees of cytotoxicity to cancer cells, with IC values ranging from 8.67 to 98.89 μmol·L.
Subject(s)
Humans , A549 Cells , Alkaloids , Chemistry , Toxicity , Antineoplastic Agents , Chemistry , Toxicity , Carbazoles , Chemistry , Toxicity , Cell Line, Tumor , Cell Survival , Clausena , Chemistry , HeLa Cells , Molecular Structure , Plant Extracts , Chemistry , Toxicity , Plant Leaves , Chemistry , Plant Stems , Chemistry , Plants, Medicinal , ChemistryABSTRACT
The present study carried out a phytochemical investigation of the methanol extract of the branches and leaves of Clausena lansium and afforded nine carbazole alkaloids (compounds 1-9) including two new carbazole alkaloids, claulansiums A and B (compounds 1 and 2). The new compounds were elucidated on the basis of extensive spectroscopic data (MS, NMR, IR, and UV) and the known compounds were identified by comparing spectroscopic data with those reported in literature. All the isolated compounds were tested for their cytotoxic activity against A549 and Hela cancer cell lines. Our results showed that compounds 2-6 exhibited varying degrees of cytotoxicity to cancer cells, with IC values ranging from 8.67 to 98.89 μmol·L.
Subject(s)
Humans , A549 Cells , Alkaloids , Chemistry , Toxicity , Antineoplastic Agents , Chemistry , Toxicity , Carbazoles , Chemistry , Toxicity , Cell Line, Tumor , Cell Survival , Clausena , Chemistry , HeLa Cells , Molecular Structure , Plant Extracts , Chemistry , Toxicity , Plant Leaves , Chemistry , Plant Stems , Chemistry , Plants, Medicinal , ChemistryABSTRACT
BACKGROUND/AIMS: To reveal possible factors predicting the effect of adefovir dipivoxil (ADV) treatment on chronic hepatitis B (CHB) and optimize the utilization of ADV. METHODS: In total, 168 treatment-naive CHB patients were enrolled, including 117 hepatitis B e antigen (HBeAg)-positive patients and 51 HBeAg-negative patients who met the inclusion criteria. All patients were treated with ADV 10 mg per day for 48 weeks. Multiple logistic regression analyses were used to investigate baseline factors, and responses at weeks 12 and 24 were analyzed as predictive values. RESULTS: Multiple regression analyses showed that baseline HBeAg status and HBV DNA levels significantly affected the virological response (VR) (p or =10(3) copies/mL at week 24 is observed.
Subject(s)
Humans , Adenine , Body Mass Index , DNA , Hepatitis B , Hepatitis B e Antigens , Hepatitis B, Chronic , Hepatitis, Chronic , Logistic Models , OrganophosphonatesABSTRACT
OBJECTIVE To investigate the risk factors,clinical characteristics and resistance of Staphylococcus aureus nosocomial infections so as to guide the treatment of S.aureus infection.METHODS To collect clinical materials of S.aureus nosocomial infection and analyze risk factors and clinical characteristics and detect sensitivity of isolated strains to antibacterial agents.RESULTS Severe underlying diseases existed among 73 cases of S.aureus nosocomial infections,82.19 percent of patients had received invasive interventions.Lower respiratory tract was the most common infective site.Seventy-nine strains of S.aureus were isolated,including 66 meticillin-resistant S.aureus(MRSA) and 13 meticillin-sensitive S.aureus(MSSA).S.aureus showed general resistance to many kinds of antibiotic drugs.The resistant rates of MRSA were much higher than those of MSSA(P