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1.
Chinese Journal of Experimental Traditional Medical Formulae ; (24): 197-205, 2024.
Article in Chinese | WPRIM | ID: wpr-1006285

ABSTRACT

Diabetic retinopathy(DR) and coronary heart disease(CHD) are both major chronic vascular complications that seriously jeopardize the health of the population and often occur together in clinical practice, it is of great clinical value to actively explore the association between the two in the process of disease development and methods of prevention and treatment of modern medicine and traditional Chinese medicine(TCM). According to TCM, the heart and eyes physiologically communicate with each other by taking Qi, blood and veins as bridges, blood stasis obstructing collaterals is the common TCM etiology of DR and CHD, whose mechanism involves inflammation, oxidative stress and endothelial dysfunction. Promoting blood circulation and removing blood stasis plays an important role in the same treatment for different diseases and prevention and treatment of comorbidities, possibly by inhibiting the expression of interleukin-1β(IL-1β), endothelin-1(ET-1) and hypoxia inducible factor-1α/vascular endothelial growth factor(HIF-1α/VEGF), regulating phosphatidylinositol 3-kinases/protein kinase B/mammalian target of rapamycin(PI3K/Akt/mTOR) pathway, initiating adenosine monophosphate(AMP)-activated protein kinase/silent information regulator 1(AMPK/SIRT1) and nuclear transcription factor erythroid 2-related factor 2/heme oxygenase-1(Nrf2/HO-1) signaling pathways, inhibiting Hippo/Yes-associated protein(Hippo/YAP) signaling pathway, inhibiting mitochondrial permeability transition pore and anti-platelet agglutination for treating DR and CHD, which provides a multi-component, multi-pathway and multi-target selection strategies and ideas for the prevention and treatment of DR and CHD by TCM from a biological perspective. Based on this, subsequent studies should focus on constructing clinically relevant comorbidity models, conducting multicenter prospective studies, and fully utilizing artificial intelligence technology to gain a deeper understanding of the relationship between the two diseases, so as to elucidate the mechanism of promoting blood circulation and removing blood stasis in preventing and treating panvascular diseases.

2.
Chinese Journal of Experimental Traditional Medical Formulae ; (24): 112-119, 2024.
Article in Chinese | WPRIM | ID: wpr-999167

ABSTRACT

ObjectiveTo observe and compare the electrocardiogram index, myocardial morphology, and connexin 43 (Cx43) expression of two rat models of acute cerebral infarction (ACI) due to stasis combined with toxin complicated with cerebral-cardiac syndrome (CCS), and to provide experimental evidence for the research on the occurrence mechanism of cardiac diseases induced by ACI and the clinical diagnosis and treatment of CCS. MethodSixty SPF-grade male SD rats were randomized into six groups (n=10): normal , syndrome of stasis combined with toxin induced by carrageenin combined with dry yeast (CA/Y), multi-infarct induced by micro-embolism (ME), middle cerebral artery occlusion (MCAO), CA/Y+ME, and CA/Y+MCAO groups. The model of syndrome of stasis combined with toxin was established by intraperitoneal injection with carrageenan (CA) at 10 mg·kg-1 on the first day and subcutaneous injection with dry yeast (Y) suspension (2 mg·kg-1) on the second day of modeling. Twenty-four hours after the modeling of ACI, the electrocardiograms (ECGs) of rats in each group were collected and the number/percentage (%) of abnormal ECG was calculated. The infarct area of the brain was evaluated by 2,3,5-triphenyltetrazolium chloride (TTC) staining, and myocardial injury was assessed by hematoxylin-eosin (HE) staining. Immumohistochemical staining and Western blot were employed to determine the expression of Cx43 in the myocardium. ResultA certain number of rats in each model group presented abnormal ECG. Compared with the normal group and CA/Y group, CA/Y+MCAO group had the highest rate of abnormal ECG (P<0.01). Compared with the normal, CA/Y, ME, and CA/Y+ME groups, the CA/Y+ME and CA/Y+MCAO groups showed decreased amplitudes of P-wave and T-wave, shortened P-R interval, and extended Q-T interval, which were particularly obvious in the CA/Y+MCAO group (P<0.05, P<0.01) and in accordance with the cerebral infarction area and pathological changes. The expression of Cx43 was up-regulated in both CA/Y+ME and CA/Y+MCAO groups, especially in the CA/Y+MCAO group (P<0.01). ConclusionThe two rat models of ACI due to stasis combined with toxin complicated with CCS can be used to study the mechanism of heart diseases caused by cerebrovascular diseases and the therapeutic effects of Chinese medicines with the functions of resolving stasis and detoxifying. Moreover, the CA/Y+MCAO method has higher abnormal electrocardiogram rate, severer myocardial pathological injury, and higher expression of Cx43 protein. The models can be chosen according to specific experimental purpose.

3.
Rev. bras. med. esporte ; 29: e2022_0715, 2023. tab
Article in English | LILACS-Express | LILACS | ID: biblio-1423303

ABSTRACT

ABSTRACT Introduction: Volleyball consists mainly of aerobic activities, causing positive impacts on cardiopulmonary resistance and the physical fitness of college students. Studying scientifically the training impacts of this sport can provide a scientific reference to support student training. Objective: Study the effects of volleyball training on college students' physical fitness and cardiopulmonary endurance. Methods: Second-year physical education students at a university were randomly selected for the experiment (n=50). Divided into the experimental group - adopting volleyball training and practice and control - with traditional athletics, they underwent a full semester of targeted physical activities. Physical fitness and cardiopulmonary endurance tests were performed before and after the experiment, and the results of the test batteries were cataloged and statistically confronted. Results: The experimental class students' results were superior in the physical fitness tests(P<0.05). The difference in the impact on final inter-group cardiopulmonary resistance was not so evident, demonstrating that both interventions resulted in good conditioning. Conclusion: Volleyball training positively impacted college students' physical fitness and cardiopulmonary endurance. It also increased the students' interest in the sport, optimizing the college students' physical quality and improving their cardiopulmonary resistance. Level of evidence II; Therapeutic studies - investigating treatment outcomes.


RESUMO Introdução: O voleibol é constituído principalmente por atividades aeróbicas, causando impactos positivos sobre a resistência cardiopulmonar e aptidão física dos estudantes universitários. Estudar cientificamente os impactos do treinamento desse esporte pode fornecer uma referência científica para embasar o treinamento estudantil. Objetivo: Estudar os efeitos do treinamento de voleibol sobre a aptidão física e a resistência cardiopulmonar dos estudantes universitários. Métodos: Alunos do segundo ano de educação física em uma universidade foram selecionados aleatoriamente para o experimento (n=50). Divididos em grupo experimental - adotando treinamento e prática de voleibol e controle - com atletismo tradicional, foram submetidos a um semestre completo de atividades físicas direcionadas. Testes de aptidão física e resistência cardiopulmonar foram executados antes e após o experimento, os resultados das baterias de testes foram catalogados e confrontados estatisticamente. Resultados: Os resultados dos alunos da classe experimental foram superiores nas provas de aptidão física (P<0.05). A diferença no impacto sobre a resistência cardiopulmonar final inter-grupo não foi tão evidente, demonstrando que ambas intervenções acarretaram em um bom condicionamento. Conclusão: O treinamento de vôlei apresentou impactos positivos sobre a aptidão física e a resistência cardiopulmonar dos estudantes universitários. Também aumentou o interesse dos alunos pelo esporte, otimizando a qualidade física dos estudantes universitários e melhorando a sua resistência cardiopulmonar. Nível de evidência II; Estudos terapêuticos - investigação dos resultados do tratamento.


RESUMEN Introducción: El voleibol está constituido principalmente por actividades aeróbicas, causando impactos positivos en la resistencia cardiopulmonar y en la aptitud física de los estudiantes universitarios. Estudiar científicamente los impactos del entrenamiento de este deporte puede proporcionar una referencia científica en la que basar el entrenamiento de los alumnos. Objetivo: Estudiar los efectos del entrenamiento de voleibol sobre la aptitud física y la resistencia cardiopulmonar de estudiantes universitarios. Métodos: Para el experimento se seleccionaron aleatoriamente estudiantes de segundo curso de educación física de una universidad (n=50). Divididos en grupo experimental -que adoptó entrenamiento y práctica de voleibol- y control -con atletismo tradicional-, se sometieron a un semestre completo de actividades físicas dirigidas. Se realizaron pruebas de aptitud física y resistencia cardiopulmonar antes y después del experimento, se catalogaron los resultados de las baterías de pruebas y se confrontaron estadísticamente. Resultados: Los resultados de los alumnos de la clase experimental fueron superiores en las pruebas de aptitud física (P<0,05). La diferencia en el impacto sobre la resistencia cardiopulmonar final intergrupos no fue tan evidente, lo que demuestra que ambas intervenciones dieron lugar a un buen acondicionamiento. Conclusión: El entrenamiento de voleibol presentó impactos positivos sobre la aptitud física y la resistencia cardiopulmonar de los estudiantes universitarios. También aumentó el interés de los alumnos por el deporte, optimizando la calidad física de los universitarios y mejorando su resistencia cardiopulmonar. Nivel de evidencia II; Estudios terapéuticos - investigación de los resultados del tratamiento.

4.
Rev. bras. med. esporte ; 29: e2022_0701, 2023. tab
Article in English | LILACS-Express | LILACS | ID: biblio-1423519

ABSTRACT

ABSTRACT Introduction: Traditional physical training has a good effect on the improvement of strength and quality indicators of the athletes; however, recent pieces of evidence indicate that relating it to high-intensity interval training may reduce the physical problems of its practitioners, besides accelerating the physical skills required by volleyball. Objective: Study the application of high-intensity interval training on the physical ability of volleyball players. Methods: 40 volunteers were recruited, among freshmen and university students, practicing volleyball. They were divided equally into the control and experimental group. The control group used traditional physical training based on aerobic exercise, while the experimental group adopted a high-intensity interval training program. Each session lasted 1.5 hours, twice a week, for 9 weeks. Relevant physical and functional data were individually collected before and after the intervention, compared, and statistically analyzed. Results: There was an increase in the quality of the long-distance throw, horizontal pull force, 800 m run, 30 m run, cross jump, and repeated crossing experiment. Conclusion: The high-intensity interval training protocol raised the fitness level of volleyball players, allowing optimization of performance on the court. Level of evidence II; Therapeutic studies - investigation of treatment outcomes.


RESUMO Introdução: O treinamento físico tradicional apresenta um bom efeito na melhoria da força e dos indicadores de qualidade dos atletas, porém evidências recentes indicam que relaciona-lo ao treinamento intervalado de alta intensidade possa reduzir os problemas físicos de seus praticantes além de acelerar as habilidades físicas requeridas pelo voleibol. Objetivo: Estudar a aplicação do treinamento intervalado de alta intensidade sobre a habilidade física dos praticantes de voleibol. Métodos: Foram recrutados 40 voluntários entre calouros e alunos da universidade, praticantes de voleibol. Eles foram divididos igualmente em grupo controle e experimental. O grupo de controle utilizou o treinamento físico tradicional baseado em exercício aeróbico enquanto o experimental adotou o programa de treinamento intervalado de alta intensidade. Cada sessão durou 1,5 horas, duas vezes por semana, por 9 semanas. Os dados físicos e funcionais relevantes foram individualmente coletados antes e após a intervenção, comparados e analisados estatisticamente. Resultados: Houve um aumento na qualidade do arremesso de longa distância, força de tração horizontal, corrida de 800 m, corrida de 30 m, salto transversal e no experimento de cruzamento repetido. Conclusão: O protocolo de treinamento intervalado de alta intensidade elevou o nível de aptidão física nos jogadores de vôlei, permitindo uma otimização de desempenho na quadra. Nível de evidência II; Estudos terapêuticos - investigação dos resultados do tratamento.


RESUMEN Introducción: El entrenamiento físico tradicional tiene un buen efecto en la mejora de los indicadores de fuerza y calidad de los atletas; sin embargo, recientes evidencias indican que relacionarlo con el entrenamiento interválico de alta intensidad puede reducir los problemas físicos de sus practicantes, además de acelerar las habilidades físicas requeridas por el voleibol. Objetivo: Estudiar la aplicación del entrenamiento interválico de alta intensidad sobre la capacidad física de los practicantes de voleibol. Métodos: Se reclutaron 40 voluntarios, entre estudiantes de primer año y universitarios, practicantes de voleibol. Se dividieron a partes iguales en grupo de control y grupo experimental. El grupo de control utilizó un entrenamiento físico tradicional basado en ejercicios aeróbicos, mientras que el grupo experimental adoptó un programa de entrenamiento interválico de alta intensidad. Cada sesión duró 1,5 horas, dos veces por semana durante 9 semanas. Se recogieron individualmente datos físicos y funcionales relevantes antes y después de la intervención, se compararon y se analizaron estadísticamente. Resultados: Hubo un aumento en la calidad del lanzamiento de larga distancia, la fuerza de tracción horizontal, la carrera de 800 m, la carrera de 30 m, el salto cruzado y en el experimento de cruce repetido. Conclusión: El protocolo de entrenamiento interválico de alta intensidad elevó el nivel de condición física en jugadores de voleibol, permitiendo una optimización del rendimiento en la cancha. Nivel de evidencia II; Estudios terapéuticos - investigación de los resultados del tratamiento.

5.
Chinese Journal of Experimental Traditional Medical Formulae ; (24): 220-227, 2023.
Article in Chinese | WPRIM | ID: wpr-984601

ABSTRACT

Cardiometabolic disease (CMD) is a clinical syndrome in which there is a causal relationship between metabolic abnormalities and cardiovascular damage. The incidence and mortality rates of CMD remain high despite the use of potent pharmacologic interventions and clinical therapeutic approaches. There is an urgent need for effective evidence-based comprehensive management measures to improve patients' lifespan and quality of life. From the concept of "nourishing through food" proposed in the Huangdi's Internal Classic (Huang Di Nei Jing) to the widespread application of modern dietary patterns such as dietary restriction, plant-based diets, and Jiangnan cuisine, dietary regulation plays a significant role in preventing diseases, early treatment of existing diseases, and recovery. This article systematically reviewed the traditional Chinese medicine (TCM) theory related to dietary patterns, elucidated the cutting-edge evidence and mechanisms of modern dietary patterns like dietary restriction in preventing and treating CMD, and explored the strategy of integrating TCM theory with dietary patterns, aiming to establish a new food-nutrition-medicine approach that combines traditional Chinese and western medicine and provide novel insights and directions for the clinical management of CMD.

6.
Journal of Environmental and Occupational Medicine ; (12): 871-876, 2023.
Article in Chinese | WPRIM | ID: wpr-984237

ABSTRACT

Background Few studies have investigated the association between air pollution and arterial stiffness in Chinese population, and the findings are inconsistent. The problem of multicollinearity exists when modeling multiple air pollutants simultaneously. Objective To investigate potential association between air quality index (AQI) and population brachial-ankle pulse wave velocity (baPWV) in Beijing. Methods This study retrieved medical examination data of 2971 participants from the Beijing Health Management Cohort, who were under 60 years old and not yet retired, from January 1, 2015 to December 31, 2019. The most recent medical examination data available were utilized for this analysis. AQI data from 35 air pollution monitoring sites in Beijing and meteorological data (including atmospheric pressure, air temperature, wind speed, and relative humidity) from 16 meteorological monitoring stations from January 1, 2014 to December 31, 2019 were collected. An average AQI exposure level for 365 d before the date of physical examination for each participant was computed using inverse distance weighting. Multiple linear regression analysis was employed to investigate the relationship between AQI and baPWV in Beijing, after adjusting for confounding variables including age, gender, body mass index, mean arterial pressure, high-density lipoprotein cholesterol, low-density lipoprotein cholesterol, triglycerides, fasting blood glucose, atmospheric pressure, temperature, wind speed, relative humidity, medication history of diabetes, medication history of hypertension, cardiovascular disease, education, smoking status, drinking status, and physical activity intensity. Subgroup analysis was performed by age, sex, presence of diabetes, and presence of hypertension. Results AQI demonstrated an overall decreasing trend during the study period and was lower in the northern regions and higher in the southern regions of Beijing. After adjusting the confounding variables, each 10 unit increase in AQI was associated with 6.18 (95%CI: 1.25, 11.10) cm·s−1 increase in baPWV in all participants, 8.05 (95%CI: 2.32, 13.79) cm·s−1 increase in the participants <50 years, 15.82 (95%CI: 8.33, 23.31) cm·s−1 increase in the female group, 10.10 (95%CI: 4.66, 15.55) cm·s−1 increase in the participants without diabetes, and 9.41 (95%CI: 4.21, 14.62) cm·s−1 increase in the participants without hypertension. However, there was no statistically significant association observed between AQI and baPWV in the age group ≥50 years, the male group, the diabetic group, and the hypertensive group (P>0.05). Conclusion An increase in long-term AQI levels is associated with an elevation in the degree of arterial stiffness. Individuals under 50 years old, females, without hypertension or diabetes are susceptible populations to arterial stiffness when being exposed to air pollution. Improving air quality may contribute to prevent arterial stiffness.

7.
Chinese Journal of Radiology ; (12): 741-747, 2023.
Article in Chinese | WPRIM | ID: wpr-993001

ABSTRACT

Objective:To investigate the regional homogeneity (ReHo) among the major depressive disorder patients without mixed features (MDD noMF), major depressive disorder with mixed features (MMF), bipolar disorder with mixed features (BMF) and bipolar disorder patients without mixed features (BD noMF) patients, and to explore the brain activity and functional connectivity patterns of the MMF and BMF patients. Methods:This was a cross-sectional study. The MDD noMF patients (MDD noMF group), MMF patients (MMF group), BMF patients (BMF group), BD noMF patients (BD noMF group), and age-and gender-matched healthy controls (HC group) were recruited from Beijing Anding Hospital, Capital Medical University between April, 2021 and June, 2022. All the participants underwent resting-state functional MRI scanning. The ReHo values was computed with the DPABI software based on the MATLAB. Firstly, the difference in ReHo among the patients with MDD noMF, MMF, BMF, BD noMF and HC group were estimated by the analysis of covariance and the post-hoc method (LSD or Games-Howell). And then, the brain regions with significant different ReHo values were selected as the seeds to calculate the functional connectivity with the whole brain. Results:A total of 29 cases in the MDD noMF group, 24 cases in the MMF group, 26 cases in the BMF group, 29 cases in the BD noMF group, and 42 in the HC group were included. The differences in ReHo values in the left fusiform and the left precuneus of the 5 groups were statistically significant ( P<0.05). Among of them, the ReHo values of the left fusiform were lower in the MMF, BMF and BD noMF groups compared with the HC group ( P<0.05), while the ReHo values of the left precuneus in MDD noMF, MMF, BMF and BD noMF groups were higher than that in the HC group ( P<0.05). The ReHo value of the left fusiform was lower in the MMF group compared with the MDD noMF group ( P=0.001); the ReHo value of the left fusiform was lower in the BMF group compared with the MDD noMF and BD noMF groups ( P<0.05). The functional connectivity between the left fusiform and vermis, left insula, right putamen, and left medial superior frontal gyrus, and functional connectivity between the left precuneus and right superior frontal gyrus (dorsolateral) showed significant difference among the MDD noMF, MMF, BMF, BD noMF and HC groups ( P<0.05). Compared with HC group, MDD noMF, MMF, BD noMF groups showed higher functional connectivity between the left fusiform and the vermis, and MDD noMF, MMF, BMF, BD noMF group showed higher functional connectivityy between the the left fusiform and the left insula, left medial superior frontal gyrus and right putamen ( P<0.05). Compared with the MDD noMF group, the MMF, BMF and BD noMF groups showed higher functional connectivity between the left fusiform and the left insula ( P<0.05). Compared with the MDD noMF group, the BMF and BD noMF groups had higher functional connectivity between the left fusiform and the left medial superior frontal gyrus ( P<0.05). The BMF group showed higher functional connectivity of the left fusiform with the right putamen than the MDD noMF and BD noMF groups. Additonally, the BMF and BD noMF groups showed higher functional connectivity between the left precuneus and the right superior frontal gyrus (dorsolateral) than HC, MDD noMF and MMF groups ( P<0.05). Conclusions:MMF and BMF patients have local abnormalities of functional activity synchronization in the left fusiform and precuneus and abnormal functional connectivity patterns with multiple brain regions. MMF and BMF patients have specific neuroimaging features compared to MDD noMF or BD noMF patients and also share similar neuroimaging pathogenesis.

8.
Chinese Journal of Medical Education Research ; (12): 302-306, 2023.
Article in Chinese | WPRIM | ID: wpr-991310

ABSTRACT

Objective:To explore the application effect of multi-mode teaching guided by objective management in the standardized training of nursing students.Methods:A total of 39 standardized training nursing students from April 2019 to April 2020 in the department of oncology of a tertiary hospital were selected as the control group by cluster sampling method and they received traditional teaching; 40 standardized nursing students in the department of oncology from June 2020 to June 2021 were selected as the experimental group and the group adopted multi-mode teaching guided by objective management. The differences of theoretical and operational assessment scores, teaching satisfaction, critical thinking ability and nurse-patient communication ability between the two groups were studied. SPSS 20.0 was used for t-test and Chi-square test. Results:The scores of theoretical assessment [(86.17±3.74) vs. (83.92±4.93)] and operational assessment [(92.83±2.19) vs. (90.74±3.52)] in the experimental group were higher than those in the control group, and the differences were statistically significant ( P<0.05). The teaching satisfaction of the experimental group was higher than that of the control group [(46.10±2.96) vs. (42.67±2.45)], and the difference was statistically significant ( P<0.05). There were statistically significant differences in critical thinking, intellectual curiosity and analytical skills ( P<0.05). In terms of nurse-patient communication ability, except that there was no difference in collecting information, the rest were statistically significant( P<0.05). Conclusion:Multi-mode teaching guided by objective management can improve the theoretical and operational ability of nurses, nurse-patient communication ability, and teaching satisfaction.

9.
Chinese Journal of Postgraduates of Medicine ; (36): 639-645, 2023.
Article in Chinese | WPRIM | ID: wpr-991071

ABSTRACT

Objective:To investigate the relationship between n7-methylguanosine (m7G) related long non-coding RNA (lncRNA) expression and glioma prognosis, and to construct a prognosis model with m7G-related lncRNA in patients with glioma.Methods:Data related to the test set and validation set were downloaded from the Cancer and Tumor Genome Atlas (TCGA) database and the China Glioma Genome Atlas (CGGA) database. LASSO regression and random forest algorithm were used to establish the glioma prognosis model with m7G related lncRNA. Individualized risk scores were calculated using the weighted expression levels of the 12 extracted lncRNA coefficients, and test set and validation set glioma patients were categorized into high and low risk groups based on median risk score. Kaplan-Meier survival curve was drawn, the comparison method used log rank test. The efficacy of risk score in predicting the 1-, 2- and 5-year survival rate in patients with glioma was evaluated using the receiver operating characteristics (ROC) curve.Results:A total of 12 lncRNA associated with m7G were obtained, with a risk score = 1.026 × AC002454.1 + 1.086 × AC131097.4 + 1.039 × AC147651.3 + 1.01 × AGAP2-AS1 + 1.036 × CRNDE + 0.733 × GDNF-AS1 + 1.321 × HOXD-AS2 + 0.934 × LINC00641 + 1.183 × PAXIP1-AS2 + 1.258 × PVT1 + 0.909 × SOX21-AS1 + 0.754 × TTC28-AS1, with a median risk score of - 0.45 scores. Kaplan-Meier survival curve analysis result showed that the median survival time in high risk group was significantly shorter than that in low risk group (1.98 years vs. 9.51 years, log-rank χ2 = 131.78, P<0.01). ROC curve analysis result showed that the area under the curve (AUC) of risk score in predicting the 1-, 2- and 5-year survival rate in patients with glioma was 0.891, 0.923 and 0.912. In the validation set of glioma patients, Kaplan-Meier survival curve analysis result showed that the median survival time in high risk group was significantly shorter than that in low risk group (1.29 years vs. 6.88 years, log-rank χ2 = 103.27, P<0.01); ROC curve analysis result showed that the AUC of risk score in predicting the 1-, 2- and 5-year survival rate in patients with glioma was 0.724, 0.795 and 0.762. In the test set and validation set, multivariate Cox regression analysis result showed that the risk score was the independent risk factors of prognosis in patients with glioma ( HR = 1.992 and 1.247, P<0.01 or <0.05). Conclusions:A risk score model with m7G related lncRNA based on transcriptome is a novel approach to predict the prognosis of glioma patients.

10.
Chinese Journal of Blood Transfusion ; (12): 920-923, 2023.
Article in Chinese | WPRIM | ID: wpr-1004721

ABSTRACT

【Objective】 To conduct the laboratory quality assessment between 12 blood stations in Hebei province, analyze the results and explore the accuracy and comparability of testing, so as to improve the level of testing ability and quality management. 【Methods】 With reference to the external quality assessment rules of National Center for Clinical Laboratories and combined with the instructions of quality assessment samples, daily testing process of the laboratories were assessed. The quality indicators include blood cell count (WBC, RBC, Hb, HCT, MCV, MCH, MCHC and PLT), biochemical items (TP) and coagulation parameters (FIB and FⅧ). 【Results】 There are still problems in laboratories in terms of personnel operation, instrument maintenance and the impact of different reagent batches, especially in biochemical items and coagulation parameters. The pass rate of biochemical items was the lowest, only 72.75%, and that of blood cell count was the highest, reaching 98.75%. 【Conclusion】 With the progress of the project, the quality monitoring level of daily blood sampling tests in the quality control laboratory of each blood station has been improved. However, it is still necessary for each laboratory to improve the testing ability and quality management to a higher level in Hebei.

11.
Journal of Pharmaceutical Practice ; (6): 705-709, 2023.
Article in Chinese | WPRIM | ID: wpr-1003615

ABSTRACT

As a highly malignant tumor, the diagnosis of cholangiocarcinoma (CCA) is often late and the prognosis is poor for which the early symptoms are atypical and the lack of accurate biomarkers. Metabolomics is an emerging science that researches the alterations of all endogenous small molecule metabolites in an organism under the influence of pathological, physiological or genetic modification. The development and progress of CCA is closely related to metabolism. Metabolomic is characterized by global analysis, high throughput and reflects real-time alterations in biology system, providing a new avenue for biomarker screening and diseases diagnosis and treatment. The advances of metabolomics studies on CCA in the recent years were reviewed in this paper which could provide the reference for further research.

12.
Journal of Clinical Neurology ; : 589-596, 2023.
Article in English | WPRIM | ID: wpr-1000846

ABSTRACT

Background@#and Purpose We aimed to determine the clinical features of Miller Fisher syndrome (MFS) in southern China and compare them with those presenting in other countries. @*Methods@#We collected the medical records of patients diagnosed with MFS during 2013–2016.We analyzed the age, sex, onset season, precursor events, clinical symptoms and signs, findings of nerve conduction studies (NCS), cerebrospinal fluid (CSF), therapeutic remedies, nadir time, and length of hospital stay of patients with MFS in southern China. We concurrently compared the differences between urban and rural areas and between patients with incomplete ophthalmoplegia (IO) and complete ophthalmoplegia (CO). @*Results@#The study enrolled 72 patients: 36 from rural areas and 36 from urban areas, and 50 males and 22 females. The mean age at onset was 47.72 years, and 30 (41.7%) and 21 (29.2%) patients developed MFS in spring and winter, respectively. The typical triad of ophthalmoplegia, ataxia, and areflexia was observed in 50 (69.4%) patients. A history of upper respiratory tract infection 1 week before onset was found in 52.8% of the patients, while 5.6% experienced gastrointestinal infections and 48 (73.8%) exhibited albuminocytological dissociation in the CSF study. Only 26 (36.1%) patients presented abnormalities in NCS. Moreover, restricted outward eyeball movement presented in 83.5% of the patients with classic MFS and acute ophthalmoplegia, and bilateral symmetrical ophthalmoplegia presented in 64.2%. With the exception of the higher proportion of NCS abnormalities in urban areas (47.2% vs. 25.0%), urban and rural differences were insignificant regarding sex ratio, age at onset, high-incidence season, precursor events, disease characteristics, and albuminocytological dissociation in the CSF. Furthermore, patients with CO were older than those with IO (64.53±7.69 vs. 43.19±14.40 years [mean±standard deviation], p<0.001). @*Conclusions@#The patients with MFS were mostly male and middle-aged, and most presented in winter and (especially) spring. More than half of the patients had clear precursor events, most of which were classic MFS with the typical triad. More than 70% of the patients presented albuminocytological dissociation in the CSF. NCS abnormalities were uncommon in MFS. The age at onset was lower in patients with IO than in patients with CO; bilateral symmetrical extraocular muscle paralysis was the most common symptom, and the external rectus was the most frequently involved muscle.

13.
Clinical and Molecular Hepatology ; : 643-669, 2023.
Article in English | WPRIM | ID: wpr-999992

ABSTRACT

Liver organoids are three-dimensional cellular tissue models in which cells interact to form unique structures in culture. During the past 10 years, liver organoids with various cellular compositions, structural features, and functional properties have been described. Methods to create these advanced human cell models range from simple tissue culture techniques to complex bioengineering approaches. Liver organoid culture platforms have been used in various research fields, from modeling liver diseases to regenerative therapy. This review discusses how liver organoids are used to model disease, including hereditary liver diseases, primary liver cancer, viral hepatitis, and nonalcoholic fatty liver disease. Specifically, we focus on studies that used either of two widely adopted approaches: differentiation from pluripotent stem cells or epithelial organoids cultured from patient tissues. These approaches have enabled the generation of advanced human liver models and, more importantly, the establishment of patient-tailored models for evaluating disease phenotypes and therapeutic responses at the individual level.

14.
Chinese Journal of Experimental Traditional Medical Formulae ; (24): 155-162, 2023.
Article in Chinese | WPRIM | ID: wpr-996822

ABSTRACT

ObjectiveTo summarize the thinking of treatment of headache based on syndrome differentiation by reviewing the literature of materia medica in the past dynasties, so as to guide the clinical practice. MethodAll the literature of materia medica in the Chinese Medical Dictionary, involving 76 works from Han to Qing Dynasties, were searched, and the information of the herbs for treating headache was extracted. According to Chinese Materia Medica (11th Edition) and Pharmacopoeia of the People's Republic of China (2020 Edition), the nature, taste, and meridian tropism of the selected herbs were statistically analyzed, and the syndrome elements of headache were classified and counted. In addition, the contents of syndrome differentiation and treatment of headache by different herbs were extracted. ResultFrom the 76 monographs of materia medica in the past dynasties, 114 herbs for treating headache were selected. The herbs mainly had cold or warm nature, pungent or bitter taste, and tropism to the lung and live meridians. The syndrome elements of headache treated by the herbs mainly included wind attack, fire disturbance, turbid obstruction, stagnation, cold coagulation, and healthy Qi deficiency. ConclusionHeadache is mainly treated with the herbs with the effects of dispelling pathogenic wind, clearing heat and purging fire, eliminating phlegm and resolving dampness, regulating Qi movement and activating blood, warming Yang and dispelling cold, and tonifying deficiency and reinforcing healthy Qi, and the herbs are often used in combinations. Headache is treated following the principles of dispelling wind and pathogen, regulating Qi and blood, and tonifying deficiency and purging excess, which is in line with the laws of obstruction and nutrient deficiency causing pain.

15.
Chinese Journal of Nephrology ; (12): 305-311, 2023.
Article in Chinese | WPRIM | ID: wpr-994979

ABSTRACT

In recent years, with the development of metabolic reprogramming research, people have changed their understanding of the biological effects of immune cells. Under the stimulation of inflammatory response, immune cells re-regulate their metabolism and bioenergetics, provide energy and substrates for cell survival, and initiate immune effect functions. Nod-like receptor protein 3 (NLRP3) inflammasome, as an important component of the innate immune system, has been shown to sense metabolites such as uric acid and cholesterol crystals, and can be inhibited by metabolites such as ketones. It is also regulated by mitochondrial reactive oxygen species and glycolytic components (such as hexokinase). Recent studies have shown that a variety of metabolic pathways converge as effective regulators of NLRP3 inflammasome. The paper reviews the metabolic regulatory pathways and specificity of NLRP3 inflammasome activation, and its role in renal diseases.

16.
STOMATOLOGY ; (12): 88-91, 2023.
Article in Chinese | WPRIM | ID: wpr-965347

ABSTRACT

@#Chondroitin sulfate is an important component of extracellular matrix (ECM) in animal and human body. In recent years, chondroitin sulfate has been proven to have potential efficacy in biomedical application and has been widely used in bone regeneration and osteogenesis, especially in craniofacial reconstruction and dental medicine. Research shows that chondroitin sulfate derivatives and chondroitin sulfate composite scaffolds have great potential in promoting osteogenesis and biomineralization. However, due to the variety of chondroitin sulfate and various application forms, study on its mechanism of osteogenic repair is still insufficient. In this paper, biological characteristics, bone regeneration and osteogenesis of chondroitin sulfate, its application in different biomaterial design and future prospect are discussed.

17.
Journal of Pharmaceutical Practice ; (6): 119-124, 2023.
Article in Chinese | WPRIM | ID: wpr-964284

ABSTRACT

Objective To optimize the microwave-assisted extraction process of green tea polyphenols. Methods The extraction yield of tea polyphenols was figured up by building the standard curve of gallic acid and examining the concentration of tea polyphenols in green tea extract with the introduction of a correction factor. The effects of four single factor levels of microwave extraction time, microwave output power, liquid-to-material yield, and ethanol volume fraction on the extraction yield of tea polyphenols were primarily studied in this experiment. The response surface was applied to further optimize the extraction process of green tea polyphenols after exploring the appropriate range of four single factor levels. Results The optimal extraction process was as follows: extraction time 37 s, microwave output power 350 w, material - liquid yield 1∶45 (g/ml), ethanol volume fraction 55%, and the actual extraction yield of tea polyphenols was 25.65%, which was not much different from the theoretical value. Conclusion The microwave-assisted green tea polyphenol extraction process optimized by response surface methodology is time-saving and practicable, and the extraction yield is high.

18.
Chinese Journal of Experimental Traditional Medical Formulae ; (24): 183-189, 2023.
Article in Chinese | WPRIM | ID: wpr-980188

ABSTRACT

Vascular complications are the primary cause of the high disability and mortality in diabetic patients. Vascular calcification is a pathological basis of diabetic vascular complications and increases the risk of adverse cardiovascular events and the difficulty of revascularization in diabetic patients. It is of great clinical value to explore the measures for prevention and treatment of diabetic vascular calcification with integrated traditional Chinese and Western medicine. This paper explores the intrinsic association of stasis, toxin, and deficiency with diabetic vascular calcification to reveal the pathogenesis of diabetic vascular calcification. Stasis and toxin are causally affected by and combined with each other; deficiency refers to the deficiency of healthy Qi and the loss of Qi and blood. The three elements are associated with the occurrence and development of blood vessel diseases. This paper proposes the evolutional law of stasis, toxin, and deficiency in traditional Chinese medicine (TCM) for diabetic vascular calcification. Specifically, diabetic vascular calcification is rooted in the stasis of meridians and collaterals, develops due to the combination of stasis and toxin, and is aggravated by middle Qi deficiency. Furthermore, this paper proposes the TCM intervention principle of activating blood, removing toxin, tonifying deficiency, and dredging collaterals for the prevention and treatment of diabetic vascular calcification. The aim is to provide a theoretical basis for clinical and translational research on the prevention and treatment of diabetic vascular calcification with integrated Chinese and Western medicine.

19.
Chinese Journal of Experimental Traditional Medical Formulae ; (24): 72-78, 2023.
Article in Chinese | WPRIM | ID: wpr-973747

ABSTRACT

ObjectiveTo screen and establish animal models of combined stasis and toxin syndrome based on the comparison of three modeling methods, i.e., carrageenan (Ca), Ca combined with dried yeast (Ca+Yeast), and Ca combined with lipopolysaccharide (Ca+LPS). MethodForty SPF male SD rats were randomly divided into normal group, Ca group, Ca+Yeast group, and Ca+LPS group, with 10 rats in each group. The Ca group, Ca+Yeast group, and Ca+LPS group received an intraperitoneal injection of Ca (10 mg·kg-1) on the first day. The Ca+LPS group received an intraperitoneal injection of LPS (50 μg·kg-1) on the second day, and the Ca+Yeast group received a subcutaneous injection of dry yeast suspension (2 mg·kg-1) on the back on the second day. The rectal temperature of each group was dynamically observed after modeling. After 24 hours of modeling, the macroscopic evaluation indexes, including tongue manifestation, pulse, and black tail length in each group were observed. The PeriCam PSI imaging system was used to detect the blood flow perfusion of the rat tail. The automatic hemorheology analyzer was used to measure the whole blood viscosity and plasma viscosity of each group. The PL platelet function analyzer was used to detect the platelet aggregation rate of the rats. The enzyme-linked immunosorbent assay (ELISA) was used to detect the interleukin-6 (IL-6) level in the rat plasma. The myocardial tissue, brain tissue, and lung tissue of each group of rats were observed by hematoxylin-eosin (HE) staining. ResultCompared with the normal group, all three model groups showed varying degrees of black tail (P<0.05, P<0.01), reduced blood flow perfusion at the tail end (P<0.05, P<0.01), decreased R, G, and B values of tongue manifestation (P<0.05, P<0.01), and increased maximum platelet aggregation rate (P<0.05, P<0.01). The pulse amplitudes of the Ca+Yeast group and the Ca+LPS group were lower than that of the normal group (P<0.05, P<0.01). In addition, the average rectal temperature of the Ca+Yeast group increased after 24 hours of modeling (P<0.01), and the low-, medium-, and high-shear whole blood viscosity and plasma viscosity increased (P<0.05, P<0.01) as compared with those in the normal group. Additionally, the expression level of the plasma inflammatory factor IL-6 was significantly up-regulated (P<0.05). Pathological morphology results showed that the Ca+Yeast group had the most severe pathological changes, with small foci of myocardial fiber dissolution, inflammatory cell infiltration, and fibroblast proliferation observed. In the hippocampal area, the neurons were sparse and had undergone red degeneration. In the small focus of the lung interstitium, lymphocytes and neutrophils were infiltrated. ConclusionThe animal model of combined stasis and toxin syndrome was properly established using Ca+Yeast. The systematic evaluation system of the model, which includes traditional Chinese medicine four diagnostic information, western medicine microscopic indicators, and tissue pathological morphology, is worthy of consideration and reference by researchers.

20.
Chinese Journal of Experimental Traditional Medical Formulae ; (24): 185-193, 2023.
Article in Chinese | WPRIM | ID: wpr-960922

ABSTRACT

ObjectiveTo discuss the thought of treatment of orifices in the Chinese herbal classics in the past dynasties based on the correspondence between drugs and symptoms to guide the clinical treatment based on syndrome differentiation. MethodAll the literature data of Chinese herbal classics were retrieved from the database of the Chinese Medical Dictionary, involving 76 works of Chinese herbal classics and covering representative works from the Han dynasty to the Ming and Qing dynasties. The information on Chines herbal drugs for the treatment of orifices was collected and sorted out. According to Chinese Materia Medica (11th Edition) and Pharmacopoeia of the People's Republic of China (2020 Edition), the nature, flavor, and meridian tropism of the selected Chinese herbal drugs were statistically analyzed. The pathogenesis elements in the treatment of orifices were classified and counted, and the contents of syndrome differentiation and treatment in various Chinese herbal classics were extracted. ResultIn 76 Chinese herbal classics in the past dynasties, 93 Chinese herbal drugs for the treatment of orifices were selected. The nature of drugs was mainly warm, followed by cold and mild. The flavor was mainly pungent, followed by bitter and sweet. In terms of meridian tropism, drugs mainly acted on the lung meridian, followed by stomach, heart, liver, spleen, and kidney meridians. The pathogenesis elements of orifices could be divided into six categories, i.e., wind invasion, turbid obstruction and Qi stagnation, water and dampness stagnation, blood stasis and collaterals blockage, heat and toxin damage, deficiency of vital Qi and cold coagulation. ConclusionOrifices are mainly treated with drugs effective in dispelling wind and pathogenic factors, resolving turbidity and removing stagnation, inducing diuresis and eliminating dampness, promoting blood circulation and dredging collaterals, clearing heat and purging fire, tonifying deficiency and dispelling cold, which are used in combination. Eliminating pathogenic factors and dredging, tonifying deficiency and purging excess are the main characteristics of treatment of orifices based on syndrome differentiation, which is in line with the physiological dysfunction state of orifices in losing the function, evil Qi blockage and healthy Qi deficiency.

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