ABSTRACT
OBJECTIVE： To establish a method for simultaneous determination of 7 active constituents in Xiaoshuan enteric-coated capsules， such as chlorogenic acid， amygdalin， paeoniflorin， ferulic acid， senkyunolide Ⅰ， calycosin glycoside and ligustilide. METHODS： HPLC method was adopted. The determination was performed on Agilent Eclipse Plus C18 column with mobile phase consisted of acetonitrile-0.1％ phosphoric acid （gradient elution）. The detection wavelengths were set at 326 nm for chlorogenic acid， 210 nm for amygdalin， 230 nm for paeoniflorin， 321 nm for ferulic acid， 334 nm for senkyunolideⅠ， 274 nm for calycosin glycoside and 260 nm for ligustilide. The flow rate was set at 1.0 mL/min， and the column temperature was 35 ℃. The sample size was 10 μL. RESULTS： The linear range was 1.16-34.81 μg/mL for chlorogenic acid， 1.85-55.48 μg/mL for amygdalin， 13.22-396.50 μg/mL for paeoniflorin， 13.50-405.09 μg/mL for ferulic acid， 1.75-52.51 μg/mL for senkyunolideⅠ， 2.74-82.18 μg/mL for calycosin glycoside， 7.67-230.07 μg/mL for ligustilide （r＝0.999 1-0.999 7）， respectively. The limit of quantity were 0.056， 0.103， 0.085， 0.013， 0.136， 0.184 and 0.276 μg. RSDs of precision， stability （24 h） and reproducibility tests were lower than 2.0％ （n＝6）. Average recoveries were 99.3％，98.6％，98.8％，99.7％，97.1％，97.6％ and 99.2％ （RSD＜2.0％， n＝6）. CONCLUSIONS： Established method is accurate and simple. It can be used for simultaneous determination of 7 constituents in Xiaoshuan enteric-coated capsules.
ABSTRACT
Aim To explore the effect of the main active components combination of Astragalus promoting hematopoiesis on mouse model of bone marrow hematopoietic suppression. Methods The contents of five main active components such as ferulic acid, astragalo-side IV, formononetin, calycosin and calycosin glycoside were determined after the extract of Astragalus combined with Angelica at 1 : 1 ratio was prepared by water extraction, thus ascertaining the combination dosage. Mice were randomly divided into blank control, model, active component alone, five active components' combination and Astragalus-Angelica combination groups. The blank control group was given solvent for 7 days; the model group was given the intragastric administration as above and received intraperitoneal injection of cyclophosphamide on day 3 for 3 days; each drug group was treated intragastrically and established the model as above. The peripheral blood, the content of serum hematopoietic growth factor and the colony forming of hematopoietic progenitor cells were measured. Results The active components' combination and Astragalus-Angelica combination could significantly increase the numbers of peripheral blood leukocytes, e-rythrocytes, platelets, the content of hemoglobin and the area of bone marrow hematopoietic tissue (P < 0. 05 or 0. 01 ) five active components could promote the recovery of erythrocytes and hemoglobin; ferulic acid could promote the recovery of leukocyte number and bone marrow hematopoietic tissue area; ferulic acid and astragaloside IV could promote the recovery of platelet. The contents of granulocyte-macrophage colony-stimulating factor ( GM-CSF ), thrombopoietin (TPO), erythropoietin ( EPO) in serum significantly increased in Astragalus-Angelica combination and the active components combination groups ( P < 0. 05 or 0. 01); five components could increase TPO content, ferulic acid and calycosin could increase the contents of GM-CSF and EPO, calycosin glycoside could increase EPO. Astragalus-Angelica combination and the active components combination could significantly increase the colony numbers of granulocyte-monocyte colony fo-ming unit ( CFU-GM ) , megakaryocyte colony forming unit (CFU-MK), erythroid colony forming unit (CFU-E) , burst forming unit-erythroid (BFU-E) ; except for astragaloside IV, the others could increase the number of CFU-GM, five active components could all increase the numbers of CFU-MK, CFU-E, but only ferulic acid could increase the number of BFU-E. Conclusions The main active substances that the combination of Astragalus and Angelica enriches the blood are the above five active components, which exert the synergistic effect through acting on different links of hematopoiesis such as increasing the content of hematopoietic growth factor in blood, promoting the proliferation of hematopoietic progenitor cells,and so on.
ABSTRACT
OBJECTIVE:To establish a method for the simultaneous determination of flavonoids components in Astragali Ra-dix,and to explore the relationship among flavonoids components,varieties,origins and planting patterns. METHODS:HPLC was performed on the column of Venusil ASB with mobile phase of acetonitrile-0.3% formic acid (gradient elution) at a flow rate of 1.0 ml/min,detection wavelength was 260 nm,and column temperature was 25 ℃. Medicinal material quality of Astragalus mem-branaceus (Fisch.) Bge. var. mongholicus (Bge.) Hsiao and A. membranaceus (Fisch.) Bge of wild and cultivated from different province was compared. RESULTS:The linear range of the mass concentration was 0.008 9-2.224 mg/ml for calycosin glucoside (r=0.999 5),0.005 2-1.3 mg/ml for ononin(r=0.999 6),0.002 8-0.697 6 mg/ml for calycosin(r=0.999 9)and 0.002-0.5 mg/ml for formononetin (r=0.999 9);RSDs of precision,stability and reproducibility tests were lower than 1%;recoveries were 99.52%-100.74%(RSD=0.41%,n=6)for calycosin glucoside,98.84%-100.60%(RSD=0.60%,n=6)for ononin ,98.47%-101.74%(RSD=1.08%,n=6)for calycosin,100.10%-101.59%(RSD=0.32%,n=6)for formononetin. In terms of varieties,the contents of calycosin glycosides,ononin and flavonoids in A. membranaceus(Fisch.)Bge. var. mongholicus(Bge.)Hsiao were higher than those of A. membranaceus (Fisch.)Bge,but the contents of calycosin and formononetin were less than those of A. membranaceus (Fisch.)Bge;in terms of origins,calycosin glycosides and flavonoids of Inner Mongolia and Shanxi held the highest contents,fol-lowed by those of Northeast China and Gansu,and lowest in Shandong,Anhui and Shaanxi;in terms of planting patterns,the con-tents of calycosin glycosides,ononin and flavonoids of wild Astragali Radix were higher than those of cultivated varieties,and the contents of calycosin and formononetin of cultivated varieties were higher than those of wild ones. CONCLUSIONS:The method is simple,stable and reproducible,and can be used for the simultaneous determination of flavonoids components in Astragali Radix. The flavonoids components show great differences in Astragali Radix from different origins,and they are affected by varieties,ori-gins and planting patterns.