Novel neuroprotective peptides in the venom of the solitary scoliid wasp Scolia decorata ventralis

Background Solitary wasp venoms may be a rich source of neuroactive substances, since their venoms are used for paralyzing preys. We have been exploring bioactive constituents of solitary wasp venoms and, in this study, the component profile of the venom from a solitary scoliid wasp, Scolia decorata ventralis, was investigated through a comprehensive analysis using LC-MS. Two peptides were synthesized, and their neuroprotective properties were evaluated. Methods A reverse-phase HPLC connected to ESI-MS was used for LC-MS analyses. Online mass fingerprinting was performed from TIC, and data-dependent tandem mass spectrometry gave the MS/MS spectra. The sequences of two major peptide components were determined by MALDI-TOF/TOF MS analysis, confirmed by solid phase synthesis. Using the synthetic peptides, biological activities were assessed. Cell integrity tests and neuroprotection analyzes using H2O2 as an oxidative stress inducer were performed for both peptides. Results Online mass fingerprinting revealed that the venom contains 123 components, and the MS/MS analysis resulted in 33 full sequences of peptide components. The two main peptides, α-scoliidine (DYVTVKGFSPLR) and β-scoliidine (DYVTVKGFSPLRKA), present homology with the bradykinin C-terminal. Despite this, both peptides did not behave as substrates or inhibitors of ACE, indicating that they do not interact with this metallopeptidase. In further studies, β-scoliidine, but not α -scoliidine, showed protective effects against oxidative stress-induced neurotoxicity in PC12 cells through integrity and metabolism cell assays. Interestingly, β-scoliidine has the extension of the KA dipeptide at the C-terminal in comparison with α-scoliidine. Conclusion Comprehensive LC-MS and MS/MS analyses from the Scolia decorata ventralis venom displayed the component profile of this venom. β-scoliidine showed an effective cytoprotective effect, probably due to the observed increase in the number of cells. This is the first report of solitary wasp venom peptides showing neuroprotective activity.(AU)

Clinical analysis of 28 patients with gastrointestinal bleeding in maintenance hemodialysis / 中国基层医药

Objective To explore the reasons and clinical characteristics of gastrointestinal bleeding in maintenance hemodialysis patients.Methods Clinical data of 28 hemodialysis patients combined with gastrointestinal bleeding were retrospectively analyzed.The reasons and clinical characteristics of gastrointestinal bleeding were analyzed.Results The incidence rate of gastrointestinal bleeding in hemodialysis patients was 20.9%,hemorrhage of upper digestive tract in 23 cases (82.1%)and hemorrhage of lower digestive tract in 5 cases (17.9%).The reasons of hemorrhage of upper digestive tract were mucosal erosion bleeding of gastric esophagus duodenum in 15 cases (65.2%)and peptic ulcer in 8 cases (34.8%).The positive rate of helicobacter pylori infection was 66.7%(12 /18).In 10 cases (35.7%),gastrointestinal bleeding happened within three months after hemodialysis.Vascular access for hemodialysis were used by central venous catheterization in 13 cases (46.4%)and arteriovenous fistula in 15 cases (53.6%).The prognosis of gastrointestinal bleeding in hemodialysis patients were continuing hemodialysis in 23 cases (82.1%),turned to peritoneal dialysis in 2 cases (7.2%)and death in 3 cases (10.7%).Conclusion The major reason of gastrointestinal bleeding in hemodialysis patients is hemorrhage of upper digestive tract.Mucosal erosion bleeding of gastric esophagus duodenum is major in hemorrhage of upper digestive tract.The positive rate of helicobacter pylori infection is higher.More than 1 /3 gastrointestinal bleeding happens within three months after hemodialysis.The prognosis of the majority of patients is good by internal medicine combined therapies and no heparin hemodialysis.

Medical equipment risk management comprehensive analysis of quality and safety / 中国医学装备

Objective: The medical equipment safety risks were avoided and reduce in clinical use. Methods: The security issues to may occur in the process of clinical diagnosis and treatment must be recognized on the multiple links of the medical equipment design, manufacture, management, and the user from the perspective of system security. From the causes of the formation of risk classification, risk assessment, and risk several aspects were analyzed, and work out preventive measures. Results:through the medical equipment quality and safety risk management, take appropriate measures to prevent the happening of the security risks, the influence of the safety risk factors is avoided and reduced. Conclusion:the medical equipment quality and safety risk problem involves many complicated factors, It must be strengthened the quality safety risk of the medical equipment comprehensive analysis, evaluation, management and control.

Meta-analysis of the influential factors on cervical cancer among married Chinese women in recent 10 years / 中国综合临床

Objective To investigate the risk factors of cervical cancer in Chinese married women in recent 10 years in order to provide evidence based approaches for cervical cancer prevention and control Methods Eight case-control studies from 2002 to 2011 were selected from research literatures by using keywords such as cervical cancer, risk factors, influential factors and case-control study, cancer, risk factors, factors andcase-control study as the search term.We adopted the Mentel-Haensel fixed effect model and Dersimonion-Laird random effect model to gain a comprehensive and quantitative assessment of cervical cancer and its risk factors.Results Among the 8 case-control studies,the total number of cases and controls were 2868 and 8045,respectively.The risk factors included human papilloma virus (HPV) (RR =5.47,95 ％ CI:3.40-8.82),family history of cervical cancer (RR =2.40,95 ％ CI:1.39-4.16),number of abortions (RR =1.74,95％ CI:1.49-2.03),first sexual intercourse age number of sexual partners (1.72,95％ CI:1.36-2.16),low cultural level (RR =1.68,95％ CI:1.18-2.40).Conclusion The major risk factors for cervical cancer among married women in China included HPV,family history of cervical cancer,number of abortions,first sexual intercourse age number of sexual partners and low cultural level.

Analysis of the different surgical treatment for inguinal hernia / 中国基层医药

ObjectiveTo explore the clinical effect of the different surgical treatment for patients with inguinal hernia.Methods98 patients with inguinal hernia were randomly divided into two groups and 49 cases in each group.The observation group was given filling tension-free hernia repair,the control group using traditional hernia repair.The postoperative follow-up were compared in clinical efficacy and recurrence.ResultsIn the observation group and control group,the duration of postoperative pain,surgical time and patient recovery time was 93.9％ (46/49) vs 81.6％ (40/49) (x2 =2.645,P＜0.01),(45.2 ±7.2)min vs (51.4 ±6.4)min(P ＜0.05),(60.8±5.4) min vs (71.4 ± 6.7) min ( P ＜ 0.05 ),( 2.0 ± 2.1 ) d vs (4.9 ± 1.8) d ( P ＜ 0.05 ).Postoperative follow-up found that both groups of patients relapse rate was statistically significant( x2 =3.279,P ＜0.01 ).ConclusionTwo different surgical treatment of inguinal hernia,the tension-free hernia repair compared with the traditional hernia repair was more suitable for the body,surgery time and recovery time was short,reducing the relapse rate in patients with inguinal hernia,was an effective means of treatment.

Current research and development of tissue engineered venous valve / 中国组织工程研究

OBJECTIVE: To review the current research of tissue engineered venous valve at home and abroad, to analyze the developing trend of tissue engineered venous valve in the clinical application.METHODS: A computer retrieve was performed among PubMed, ProQuest Health & Medical Complete database, Springer English Academic Journal Full-text database, Elsevier Full-text database between January 2000 and August 2009, with the key words of "tissue engineering venous valve", and the language was limited to English. At the same time, Chongqing VIP database, Qinghua Academic Journals Database, Chinese Biomedical Literature database were also screened on computer by using the