ABSTRACT
BACKGROUND AND OBJECTIVES: The classic immunotherapy has the inconvenience and the risk of anaphylaxis. Recent reports revealed that immunostimulatory DNA sequences (CpG motif, ISS-ODN) have been shown to act as strong Th1 response-inducing adjuvants and DNA based vaccination might be an effective therapeutic option for the treatment of allergic disease. In our study, we investigated whether ISS-ODN have anti-allergic effect in the mouse rhinitis model of Dermatophagoides farinae (Der f), the most common allergen in Korea. MATERIALS AND METHOD: C57BL/6 mice were sensitized with crude extract of Der f extract. After injection of ISS-ODN, several parameters of allergic response were evaluated. RESULTS: Injection with ISS-ODN suppressed itching symptom, eosinophilic infiltration into nasal mucosa and the splenic T cell proliferation after Der f allergen challenge in the murine model of allergic rhinitis. However, injection with ISS-ODN did not show a detectable Der f-specific IgE suppression. CONCLUSION: Our findings suggested ISS-ODN have an anti-allergic effect in the allergic rhinitis model of Der f allergen. House dust mite allergy can be reduced by the injection with ISS-ODN.
Subject(s)
Animals , Mice , Anaphylaxis , Base Sequence , Cell Proliferation , Dermatophagoides farinae , DNA , Eosinophils , Hypersensitivity , Immunoglobulin E , Immunotherapy , Korea , Nasal Mucosa , Pruritus , Pyroglyphidae , Rhinitis , VaccinationABSTRACT
Objective To identify the putative CpG-N ODNs in adenovisus 2 DNA (Adv2 DNA) and Adv5 DNA by comparing the sequence difference among Adv2, 5, 12 DNA and E.coli (EC) DNA. Methods Sequences of Adv2, 5, 12 DNA and EC DNA were obtained from the Entrez Nucleotides database at NCBI. The specific CpG motifs of Adv2 DNA and Adv5 DNA were identified after above sequences were analyzed and compared by softwares such as DNATools, BioEdit, and so on. All the 12-ODNs with specific CpG motif core were searched from Adv2 DNA and Adv5 DNA. Results 19 specific CpG motifs were ascertained and 504 12-ODNs were detected in Adv2 DNA and Adv5 DNA. Conclusion 504 12-ODNs were putative CpG-N ODNs in Adv2 DNA and Adv5 DNA.
ABSTRACT
Objective To obtain a potent bacterial DNA antagonizing CpG oligonucleotides (CpG-N ODN) from the structures of Adv2, 5 DNA. Methods Ten putative CpG-N ODNs were synthesized and investigated. Their abilities to inhibit the TNF-? release from hPBMC were observed. Based on the above results, the putative CpG-N ODN was redesigned according to the relationship between the structure and free energy using RNA structure software (version 3.71). Eleven putative CpG-N ODNs were synthesized and screened. Results Out of the ten initial CpG ODNs, ODN101 was the only CpG-N ODN with weak activity to inhibit TNF-? release from hPBMC induced by CpG-N ODN. After redesign, five CpG-N ODNs with strong activity were confirmed. Conclusion Six CpG-N ODNs were obtained with activity to inhibit TNF-? release from hPBMC induced by CpG-N ODN.