ABSTRACT
Se realizó un estudio descriptivo y transversal de la corteza cerebelosa humana, desde el punto de vista histomorfométrico, desde enero hasta septiembre del 2015, con el empleo de la microscopia holográfica digital instalada en el Departamento de Holografía Digital de la Universidad de Oriente en Santiago de Cuba, con vistas efectuar mediciones que permitieran establecer comparaciones con otros estudios. Los cálculos mostraron el grosor de las capas molecular y granulosa, el área, el perímetro, los diámetros mayores y menores del cuerpo y el núcleo de las células de Purkinje. Asimismo, se tomaron imágenes holográficas en tres dimensiones, que posibilitaron concluir la existencia de parámetros determinados mediante este procedimiento, los que no habían sido notificados y que resultan de interés en el estudio histológico de la corteza cerebelosa
A descriptive and cross-sectional study of the human cerebellar cortex, from the histomorphometric point of view, was carried out from January to September, 2015, using the digital holographic microscopy installed in the Digital Holography Department of Oriente University in Santiago de Cuba, aimed at making measurings that allowed to establish comparisons with other studies. The calculations showed the thickness of the molecular and granular layers, the area, perimeter, greatest and smallest diameters of the body and Purkinje cells nucleus. Also, holographic images in three dimensions were taken, that facilitated to conclude the existence of certain parameters by means of this procedure, those that had not been notified and are of interest in the histological study of the cerebellar cortex
Subject(s)
Humans , Male , Female , Holography , Cerebellar Cortex , Cerebellum/ultrastructure , Epidemiology, Descriptive , Cross-Sectional StudiesABSTRACT
Se efectuó una revisión bibliográfica exhaustiva sobre la microscopia holográfica digital y se ofrece una información actualizada sobre esta novedosa técnica de registro óptico en el país, así como de sus fundamentos teóricos y principales aplicaciones en el campo de la biología, para que pueda ser consultada por estudiantes y profesionales de la medicina y ciencias afines, con vistas a promover la realización de futuras investigaciones relacionadas con dicha técnica
An exhaustive literature review on the digital holographic microscopy was carried out and an updated information on this novel technique of optic record, its theoretical foundations and main uses in the field of biology in the country is offered, so that it can be consulted by students and professionals of medicine and similar sciences, aimed at promoting the realization of future investigations related to this technique
Subject(s)
Humans , Male , Female , Holography , Biological Specimen Banks , Microscopy , Specimen Handling , Microscopy, VideoABSTRACT
Se realizó una investigación descriptiva y transversal para determinar la utilidad de la microscopia holográfica digital en el estudio histomorfométrico de eritrocitos humanos, mediante el análisis comparativo con la microscopia óptica, en el Departamento de Holografía Digital de la Universidad de Oriente de Santiago de Cuba, de abril del 2014 a igual mes del 2015. A tal efecto se seleccionaron muestras de eritrocitos diluidos en las soluciones amortiguadoras Hepes y Hepa, se emplearon el programa Holodig® y el sistema Matlab® para las mediciones en la técnica holográfica, y en el procesamiento estadístico se aplicó la prueba de la t de Student para la comparación de medias entre ambas técnicas microscópicas. Se obtuvo que la microscopia holográfica digital permitiera observar los límites y contornos de los eritrocitos estudiados y las mínimas variaciones en su forma, además de realizar estudios morfométricos, cuyos resultados mostraron similitud a los obtenidos con la microscopia óptica, y de otros parámetros, como el volumen
A descriptive and cross-sectional investigation to determine the usefulness of the digital holographic microscopy in the histomorphometric study of human red blood cells, by means of the comparative analysis with the optic microscopy, was carried out in the Digital Holography Department of Oriente University in Santiago de Cuba, from April, 2014 to the same month in 2015. To such an effect, red blood cells samples diluted in the Hepes and Hepa shock-absorbing solutions were selected, Holodig program and Matlab® system were used for the measurement in the holographic technique, and in the statistical processing the Student t test was applied for the comparison of means between both microscopic techniques. It was concluded that the digital holographic microscopy allowed to observe the limits and contours of the studied red blood cells and the minimum variations in their form, besides carrying out morphometric studies which results showed similarity to those obtained with the optic microscopy, and of other parameters, as volume
Subject(s)
Erythrocytes , MicroscopyABSTRACT
Objective Galangin has various antitumor activities and digital holographic microscopy ( DHM ) .This study aimed to investigate the detection of galangin-induced apoptosis of HepG2 cells using DHM. Methods HepG2 cells were cultured in vitro and treated with galangin at 130 μmol/L for 6, 12, and 24 hours respectively to induce apoptosis.Then, MTT assay was used to detect the proliferation of the HepG2 cells, Hoechst 33342 staining and flow cytometry adopted to determine their apoptosis, and DHM employed to visualize their morphological changes. Results Compared with the blank controls, galangin significantly inhibited the proliferation of the HepG2 cells ([7.32 ±2.47]%vs [39.14 ±2.04]%, P<0.01) and obviously induced their nuclear condensa-tion and apoptosis, with a total apoptosis rate of (11.550 ±0.043)%at 24 hours.DHM showed statistically significant differences be-tween the galangin-treated and control groups in the morphological changes of the HepG2 cells at the three time points (P<0.05). Conclusion Digital holographic microscopy can be used to detect the morphological changes of HepG2 cells during galangin-induced apop-tosis.