Fluorescence in situ hybridization analysis of HER-2/neu oncogene amplification in osteosarcomas / 中华创伤骨科杂志

Objective To investigate whether HER-2/neu oneogene amplification exists in 08- teosarcomas. Methods Fluorescence in situ hybridization(FISH)and DNA probes for HER-2/neu and centromere 17 were used to examine the HER-2/neu gene amplification in samples taken from 23 patients with osteosarcoma. Results None of the 23 osteosarcoma samples showed the HER-2/neu gene amplification.Condusion Since HER-2/neu gene amplification is rare in osteosarcomas, HER-2/neu target medicine should not be applied to patients with osteosarcoma