ABSTRACT
Objective:To optimize the compatibility conditions of erigeron injection and study the stability after compatibility. Methods:An orthogonal test was employed using the quantity of insoluble particles and the content of rutin as the indices and tempera-ture, solvent and solvent amount as the influencing factors. The results were comprehensively analyzed. According to the results of the orthogonal test, three batches of samples were prepared and observed the stability in 24 hours, including the changes in the number of insoluble particles, pH and total flavonoid content. Results:The optimal compatibility conditions were as follows:the temperature was 25℃, and 250 ml 0. 9% sodium chloride injection was used as the solvent. The appearance and the insoluble particles showed no sig-nificant changes in 24 hours. The total flavonoid content was also stable in 4 hours, but decreased to some extent after then. Conclu-sion:At the clinical dose, erigeron injection in 250 ml 0. 9% sodium chloride injection can keep stable in 4 hours.
ABSTRACT
AIM:To study the chemical constituents of Erigeron Injection by HPLC. METHODS: Under the guidance of HPLC analysis,two constituents were separated and purified by the aid of column chromatography with silica gel,and identified by UV、 IR、 MS、 NMR respectively and documentary data. RESULTS: Two compounds from semi-finished products of Erigeron Injection were identified as caffeic acid(Ⅰ) and chlorogenic acid(Ⅱ). CONCLUSION: Two main peaks in HPLC chromatogram of Erigeron Injection are Ⅰ and Ⅱ.