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OBJECTIVE To determine the contents of 11 components in Xueli zhike syrup， establish its chemometric method and provide reference for its quality control. METHODS HPLC method was established to simultaneously determine the contents of amygdalin， deapi-platycoside E， platycoside E， platycodin D3， euscaphic acid， tormentic acid， maslinic acid， corosolic acid， praeruptorin A， praeruptorin B and praeruptorin E in 12 batches of Xueli zhike syrup. The quality evaluation of 12 batches of samples was performed by chemometrics. RESULTS The 11 components had good linear relationships within their respective ranges （r≥0.999 1）； RSDs of precision， reproducibility and stability （24 h） tests were all lower than 2.00%. The average recovery rates ranged 96.90%-100.01% （RSDs were all lower than 2.00%）. Cluster analysis showed that 12 batches of samples were clustered into 3 groups. Principal component analysis showed that the first two principal components could represent 88.53% information of 11 components in Xueli zhike syrup. Partial least squares-discrimination analysis showed that euscaphic acid， amygdalin and praeruptorin A were the main potential markers affecting the quality of Xueli zhike syrup. CONCLUSIONS The established method can be used to control the quality of Xueli zhike syrup.
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Objective: To study the chemical constituents of the stems and leaves of mangrove plant, Scyphiphora hydrophyllacea. Methods: The chemical constituents of S. hydrophyllacea were separated and purified by silica gel, ODS, Sephadex LH-20 gel column chromatographies and preparative HPLC. Their structures were identified by physicochemical properties, spectroscopic analysis, as well as comparisons with the data reported in literatures. Results: A total of 18 compounds were isolated from the 90% ethanol extract of the stems and leaves of S. hydrophyllacea, which were identified as pomonic acid (1), euscaphic acid (2), dammaradienyl acetate (3), erythrodiol (4), lupenyl acetate (5), 30-oxo-lupeol (6), maslinic acid (7), 23-hydroxyursolic acid (8), β-amyrenone (9), acacetin (10), chrysoeriol (11), jaceosidin (12), kumatakenin (13), isosakuranetin (14), taxifolin (15), lyoniresinol (16), medioresinol (17), and balanophonin (18). Compounds 1-9 were triterpenoids, compounds 10-15 were flavonoids and compounds 16-18 were lignans. Conclusion: All compounds are isolated from the genus Scyphiphora for the first time.
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This paper mainly summarized the chemical constituents, pharmacological activities, quality control and utilization of different medicinal parts of Rosa laevigata Michx based on systematic literature research. The various pharmacological effects and rich research on the fruits of Rosa laevigata Michx made it much more attractive for development. However, the research on the roots is comparatively weak, so further studies are needed to explore the material basis and strengthen the quality control of Rosa laevigata Michx.
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Objective: To explore the chemical constituents of traditional She medicine Rubi Radix et Rhizoma (the roots and rhizomes of Rubus chingii in family Rosaceae). Methods: The compounds were isolated and purified by various column chromatography and the structures were identified by physicochemical constant determination and spectral analysis. Results: Nine compounds were isolated and identified as β-sitosterol (1), daucosterol (2), euscaphic acid (3), 11α-euscaphic acid (4), tormentic acid (5), ellagic acid (6), gallic acid (7), ursolic acid (8), and oleanolic acid (9) from 95% ethanol extract of Rubi Radix et Rhizoma. Conclusion: All the nine compounds are isolated from Rubi Radix et Rhizoma for the first time and the compound 4 is first isolated from R. chingii.
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Objective: To study the triterpenoids from the roots of Rosa laevigata. Methods: The silica gel column chromatography was used to extract and separate the chemical constituents from the roots of R. laevigata. HPLC was used to analyze its purity, chemical and spectroscopy methods were used to determine their structures. Results: Thirteen constituents were isolated and identified as niga-ichigosides F2 (1), rosamultin (2), arjunetin (3), kaji-ichigoside F1 (4), auscaphic acid (5), cecropiacic acid 3-methyl ester (6), 2-acetyl tormentic acid (7), pomolic acid (8), 2α,3α-dihydroxyurs-12,18-dien-28-oic acid (9), 3β-E-feruloyl corosolic acid (10), fupenzic acid (11), 2-O-acetyl euscaphic acid (12), and 12,13-dihydromicromeric acid (13). Conclusion: Compounds 3, 6, 7 and 9-13 are obtained from this plant for the first time. Compounds 10, 12 and 13 are obtained from the plants of Rosa L. for the first time.
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Objective: To study the triterpenoid components of Rosa cymosa. Methods: Compounds were isolated by repeated chromatography on silica gel column. The structures were elucidated by chemical and spectroscopic methods. Results: Thirteen triterpenoids were isolated and identified as 2-oxo-pomolic acid (1), 2α, 19α-dihydroxy-3-oxo-12-ursen-28-oic acid (2), 2-acetyl tormentic acid (3), pomolic acid (4), euscaphic acid (5), arjunic acid (6), myrianthic acid (7), arjunetin (8), rosamultin (9), kaji-ichigoside F1 (10), 2α, 3α, 19α, 23-tetrahydroxy-12-ursen-28-O-β-D-glucoside (11), fupenzic acid (12), and cecropiacic acid 3-methyl ester (13). Conclusion: Compounds 1-4, 7, and 11-13 are obtained from this plant for the first time.
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Objective: To study the chemical constituents from the roots of Potentilla anserine. Methods: The chemical constituents from the roots of P. anserine were isolated and purified by various chromatographic methods and their structures were elucidated on the basis of detailed spectroscopic analysis. Results: Thirteen compounds were isolated from 75% ethanol extract of P. anserine and identified as 2, 19α-dihydroxy-3-oxours-1, 12-dien-28-oic acid 28-O-β-D-glucopyranosyl ester (1), 3β-acetoxy-19α-hydroxyursa-12-en-28-oic acid (2), 3-epi-2-oxopomolic acid (3), 2-oxopomolic acid (4), pomolic acid (5), euscaphic acid (6), arjunic acid (7), potentillanoside B (8), 2-oxopomolic acid 28-O-β-D-glucopyranoside (9), pomolic acid-28-O-β-D-glucopyranoside (10), kajiichigoside F1 (11), rosamultin (12), and adenine (13). Conclusion: Compound 1 is a new triterpenoid saponin, named as potentillanoside G, compounds 3 and 7 are isolated from the plants of genus Potentilla L. for the first time, and compounds 2 and 13 are isolated from this plant for the first time.
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Objective: To develop a quantitative analysis of multi-components by single-marker (QAMS) method for the simultaneous determination of six triterpene acid (euscaphic acid, tormentic acid, maslinic acid, corosolic acid, oleanolic acid, and ursolic acid) contents in the effective fraction of Eriobotryae Folium. Methods: Ursolic acid was used as the internal reference substance, and the relative correlation factors (RCF) of euscaphic acid, tormentic acid, maslinic acid, corosolic acid, and oleanolic acid were determined by HPLC-ELSD with good reproducibility. The contents of the five components were calculated according to the RCF, respectively. The contents of these six triterpene acids in 15 batches of effective fraction were determined by the external standard method. The rationality, feasibility, and repeatability of the QAMS method were verified by comparing the results obtained from the two different methods. Results: For the six triterpene acids, there was no significant difference between the quantitative results with the two different methods in the 15 batches. Conclusion: The method established in this research is accurate and feasible that it just needs to assay single-marker (ursolic acid) for the determination of six triterpene acids in the effective fraction of Eriobotryae Folium simultaneously. Therefore, this method could provide a new reference for the quality assess of multi components in Chinese materia medica.