Effect of Auxin and Cytokinin on Grafting and Growth of Sapling of Guava (Psidium guajava L.)

The experiment was conducted to study the effect of auxin and cytokinin on grafting and growth of sapling of guava (Psidium guajava L.) at department of Horticulture, College of Agriculture, ummedganj, KOTA, KVK Modal nursery Agriculture university Kota during the year of 2020-21.In the investigation, L-49 cultivar of guava (Psidium guajava L.) was selected as mother plant for obtaining scion bud. The foliar spray of NAA and BA (50 and 25 ppm each) either on rootstock or/and scion branch was applied 10 days prior to grafting. The most responsive treatments was foliar spray on rootstocks with BA at 25 ppm concentration. Rootstocks treated with BA (25 ppm) resulted in length of scion shoot after grafting (16.63 cm), diameter of sprouted scion (2.80 cm), diameter of rootstocks (3.41 cm), number of leaves (20.83), leaf area (24.76 cm2), number of lateral shoot (7.89) as compared to others treatments.

Influence of Different Portions of Vine and Plant Growth Regulators on Growth Parameters of Sweet Potato [Ipomoea batatas (L.) Lam.]

A field experiment was conducted to study the potentiality of different portions of vine and plant growth regulators on growth performance of sweet potato during 2019 and 2020 at vegetable block, College of Horticulture, Munirabad (Koppal), located in the northern dry zone of Karnataka. The experiment was laid out in a Factorial Randomized Complete Block Design (RCBD) with thirteen treatments. Among the two factors, the first factor was different portions of vine for planting consisting basal portion (P1), middle portion (P2) and top portion (P3) and the second factor was plant growth regulators consisting CCC @ 500 ppm (G1), CCC @1000 ppm (G2), Ethrel @ 150 ppm (G3) and Ethrel @ 300 ppm (G4). The control treatments were basal portion of vine (C1), middle portion of vine (C2) and top portion of vine(C3) all without application of growth regulator. Among the different portions of vine used for planting, the top portion of vine recorded significantly higher vine length, Vine inter nodal length, Number of branches per plant and Leaf area. Application of CCC @ 500 ppm was recorded significantly higher growth attributes. The interaction effect of top portion of vine with CCC @ 500 ppm (P3G1) was found to be significantly superior with respect to growth parameters of sweet potato.

Effect of Different Pre - Sowing Seed Treatments on Germination and Growth of Papaya (Carica papaya L.) Seedlings cv. Arka Surya

A study on effect of pre-sowing seed treatments with organics, chemicals and plant growth regulators on seed germination and seedlings growth of papaya cv. Arka Surya was carried out at YSRHU- College of Horticulture, Anantharajupeta during the year 2021 - 2022, under complete randomized design with eighteen treatments and replicated thrice. The papaya seeds were subjected to various organic, chemicals and plant growth regulators treatments. Papaya seeds soaked in KH2PO4 @ 0.5% (T7) had recorded minimum days for initiation of germination (8.67) and 50% germination (10.34). The treated seeds had also recorded maximum germination percentage (86.66), germination index (1.16), dry weight of shoots (2.00 g), tap root length (26.33 cm), number of lateral roots per seedling (22.84), dry weight of roots (0.79 g), quality index of papaya seedlings (0.47) and seedling survivability (100.00%). In addition the same treatment exhibited higher chlorophyll content (3.51 and 3.91 mg/g), photosynthetic rate (15.32 and 17.76 µmol Co2m-2s-1), transpiration rate (4.61 and 5.69 mmol m-2 s-1) and stomatal conductance (2.73 and 4.12 mol m-2 s-1) at 35 and 45 DAS, respectively. While the lowest values for germination and growth parameters were observed in treatment salicylic acid @ 2 mM (T16). Among all the treatments, KH2PO4 @ 0.5% (T7) was found to be superior to the other treatments followed by KNO3 @ 0.5% for the enhancement of germination, growth and physiological traits.

Effect of Growth Regulators on Plant Growth and Flower Yield in Marigold (Tagetes erecta)

A field experiment was carried out in the Department of Horticulture, Naini Agricultural Institute, Sam Higginbottom University of Agriculture, Technology and Sciences, Prayagraj. During rabi season (2022-2023). The aim of this study was to determine the effect of different growth regulators on plant growth, flowering and flower yield of African marigold and to estimate the economics of different treatments. This experiment was laid out in Randomized block design (RBD) with 10 treatments and each treatment replicated thrice. The treatments consist of different combinations of plant growth regulators (Gibberellic acid, Salicylic acid and Sea weed extract). Treatment T3 (Gibberellic acid @150ppm) was statistically significant compared to other treatment combination, which recorded highest plant height (44.11 cm), no. of branches (43.53), stem diameter (1.61 cm), no. of leaves (118.93), plant spread (37.17 cm2), Bud length (0.93cm), days to 1st flowering (66.07 days), Size of flower (8.77cm), Number of flower per plant (34.10), Self-life (8.67 days), single flower wt. (18.28 g) in African marigold (Tagetes erecta). The economics estimation revealed that maximum benefit cost ratio was at 2.44.

Effect of Different Doses and Scheduling Time of Plant Growth Regulators and Defoliants on Growth and Yield of Cotton (Gossypium hirsutum L.) under High Density Planting System

A field trial to determine the effect of different dosages, Scheduling time of plant growth regulators and defoliators on growth and yield of cotton under high density planting system was conducted at Regional Agricultural Research Station, Warangal during kharif-2022. The experiment consisted of eleven treatments laid out in Randomized Block Design with three replications. The treatment details were T1: Application of Mepiquat chloride (M.C) 25 g a.i ha-1 @ 40 & 55 DAE + Ethereal 2000 ppm @ 40 % boll burst, T2: M.C 25 g a.i ha-1 @ 40 & 55 DAE + Ethereal 2000 ppm @ 60 % boll burst, T3: M.C 20, 30 g a.i ha-1 @ 40, 55 DAE respectively + Ethereal 2000 ppm @ 40% boll burst T4: M.C 20, 30 g a.i ha-1 @ 40, 55 DAE respectively + Ethereal 2000 ppm @ 60% boll burst T5: M.C 20 g a.i ha-1 @ 40,55 & 75 DAE+ Ethereal 2000ppm @ 40 % boll burst, T6: M.C 20 g a.i ha-1 @ 40, 55 & 70 DAE + Ethereal 2000 ppm @ 60 % boll burst, T7: M.C 25 g a.i ha-1 @ 40, 55 & 70 DAE + Ethereal 2000 ppm @ 40 % boll burst, T8: M.C 25 g a.i ha-1 @ 40, 55 & 75 DAE+ Ethereal 2000ppm @ 60% boll burst, T9: M.C 20, 25, 30 g a.i ha-1 @ 40, 55 and 70 DAE respectively + Ethereal 2000 ppm @ 40 % boll burst, T10: M.C 20, 25, 30 g a.i ha-1 @ 40, 55 and 70 DAE respectively + Ethereal 2000 ppm @ 60 % boll burst and T11: Control .(Water spray at 40, 55 and 70 DAE). Results revealed that foliar application of M.C @ 20 g a.i ha-1 @ 40, 55 & 70 DAE in conjunction with Ethereal 2000 ppm @ 60% boll burst recorded higher seed cotton yield. Plant growth, dry matter accumulation and stalk yield were recorded highest in control plot and lowest with spraying of M.C 20, 25 and 30 g a.i ha-1 @ 40, 55 and 70 DAE respectively + Ethereal 2000 ppm @ 40 % boll burst. Among similar doses of Mepiquat chloride, all the agronomic traits of cotton crop are positively influenced with application of 2000 ppm Ethereal @ 60% boll burst. The spraying of M.C @ 20 g a.i ha-1 at 40, 55 and 70 DAE + Ethereal 2000 ppm @ 60% boll burst would be economically ideal to the farming community.

Crop Regulation Studies in guava (Psidium guajava L.) cv. L 49 under Ultra High Density Planting

A field experiment was carried out on 8 years old guava cv L 49 trees to assess the effect of pruning level and growth regulators with different combinations on vegetative growth and fruiting attributes in Ultra High Density Planting. The experiment consisted of 2 factors with 36 combinations viz., factor -1 (pruning level) P1- pruning of 25 cm of the shoot from the tip, P2- pruning of 50 cm of the shoot from the tip, P3- pruning of 75 cm of the shoot from the tip, P4 – control (without pruning). Factor -2(growth regulators) G1, G2- Potassium nitrate (1 ,2%), G3, G4- Thiourea (0.1 ,0.2%), G5, G6- Ethephon (250, 500 ppm), G7, G8- Cycocel (250, 500 ppm) and G9 – Control. Results showed that among different levels of pruning, 50cm pruning from the tip with potassium nitrate spray at 2 different concentrations was found to be the best for promoting early vegetative bud burst, fruit set and fruit yield. The increment in plant height and canopy spread in East-West and North-South were recorded more in 25 cm pruning from the tip. A greater number of shoots are visible in the P3G5 combination. When compared to other treatments, the P3G2 combination significantly increased the average index number of bud bursts and shoot length under Ultra High Density Planting.

In-vitro Response of Gladiolus (var. American Beauty) towards Plant Growth Regulators Using Cormel Explants

Gladiolus is one of the most potential bulbous cut flower crops cultivated widely in India due to its attractive and long lasting spikes. The plant is propagated vegetatively through corms and cormels, but due to low rate of multiplication, its cultivation is hindered. Also, it is grown through underground modified stems, they are more often attacked by soil borne diseases such as Fusarium corm rot. In-vitro propagation techniques, assumes significance, especially for securing rapid multiplication of quality planting propagules using dormant cormlet explants and media. The results of the study revealed that the MS growing medium supplemented with 2 mg/l BAP showed improved results in respect of sprouting of explants (54.46 %), days required for sprouting of explants (8.68 DAI) and shoot length at 30 (6.32 cm), 60 (12.40 cm) and 90 (13.21 cm) DAI. The in vitro regenerated gladiolus plantlets when placed in the rooting MS medium supplemented with 4 mg/l IBA showed earlier root initiation (8.31 DAI) and higher number of roots/shoot (5.37 DAI) as compared to the rest of the treatment combinations. Hardening medium consisting of garden soil + sand + vermicompost (1:1:1) showed greater survivability (50.98 %) of plantlets. Thus, the study has been initiated an efficient protocol for in-vitro propagation of gladiolus through cormlets cultured in MS media containing BAP and IBA as shooting and rooting plant growth regulators and subsequent acclimatization in garden soil + sand+ vermicompost medium.

Effect of Boron and Plant Growth Regulators on Growth and Yield of Zaid Cowpea (Vigna unguiculata L.)

The experiment was conducted at crop research farm in the department of agronomy during summer season in the department of agronomy during summer season of 2022 on cowpea crop. The treatments consisted of 3 levels of Boron (0.75,1,1.25 kg/ha) and Plant Growth Regulators (GA3 30 ppm, NAA 35 ppm, ABA 40 ppm ) as a foliar spray. The experiment was laid out in a Randomized block design with 9 treatments and replicated “thrice”.0.75 kg/ha as soil application combination with 30 ppm gibberellic acid as foliar spray recorded maximum plant height (69.48cm), highest plant dry weight (22.22gm), crop growth rate (3.0g/m2/day), number of nodules/plant (24.37), test weight (85.6g), number of pods/plant (15.45), number of seeds/pod (13.57), seed yield (2.68t/ha), stover yield(6.09t/ha), harvest index (30.56%). However, relative growth rate was not influenced by the application of boron and plant growth regulators and it recorded maximum in treatment 1 (Boron 0.75 kg/ha +GA3 30 ppm) (0.1005 g/g/day). 0.75 kg/ha Boron + 30 ppm gibberellic acid as foliar spray recorded highest gross return (161,040.00 ?/ha), net return (113,394.00 ?/ha), and benefit: cost ratio (2.38).

Influence of Pottasium and Plant Growth Regulators on Growth and Yield of Pearlmillet (Pennisetum glaucum (L.)

Background: Pearl millet [Pennisetum glaucum (L.) is a significant food and livestock crop in the semi-arid tropics. Pearl millet is a crucial part of the nation's food security because of its capacity to thrive in drier and less fertile regions, where other grains have a comparative disadvantage. Objectives: Effect of potassium and plant growth regulators on growth parameters and yield of pearl millet Methods: With the goal of the study effect of potassium and plant growth regulators on growth and yield of Pearl millet (Pennisetum glaucum L.) Var. NBH- 5658 under a Randomized block design(RBD) with 10 treatments (T1-T10) The experiment's findings showed that a potassium concentration of 60 kg/ha combined with an NAA concentration of 100 ppm and a triacontanol concentration of 500 ppm led to the highest plant height (221.20 cm), dry weight (49.42 cm), and yield parameters for ear head length (20.50 cm), number of grains per ear head (1970), Test weight (9.38 g), grain yield (2.72 t/ha), and stover yield (3.78 t/ha). Conclusion: Farmers found that using potassium at a rate of 60 kg/ha in conjunction with NAA at a concentration of 100 ppm and Triacontanol at a concentration of 500 ppm produced the best results, with plant dimensions of (221.20 cm) in height, (49.42 gm) in plant dry weight, (1972) grains per ear head, (9.38 g) test weight, (2.68 ta/ha) in grain yield, and (3.26 ta/ha) in stover yields, respectively.

Range of factors in the reduction of hyperhydricity associated with in vitro shoots of Salvia santolinifolia Bioss / Gama de fatores na redução da hiperidricidade associada a brotos in vitro de Salvia santolinifolia Bioss

Abstract Hyperhydricity is a serious physiological disorder and affects In vitro propagation of many plants and as well of Salvia santolinifolia. The donor material to initiate the in vitro culture was the callus taken from the in vitro shoots produced on Murashig and Skoogs (MS) medium at 4.0 mg/l BA. This callus formed numerous hyperhydric shoots on culturing upon the medium of the same composition. The aim was to systematically evaluate the effect of cytokinins (Benzyladnine (BA) and N6-(-2-isopentenyl) adenine (2iP), culture vessels magnitude, medium solidification, source of nitrogen and calcium chloride for the alleviation of hyperhydricity. In the tissue cultures of S. santolinifolia BA and 2iP induced severe hyperhydricity, when other factors i.e. culture vessels magnitude and a suitable concentration of agar, ammonium nitrate (NH4NO3), potassium nitrate (KNO3) & calcium chloride (CaCl2.2H2O) were not optimized. After 30 days' culture, we observed 83.82% hyperhydric shoots at increased level (1.5 mg/l 2iP) and 81.59% at decreased levels (1.0 mg/l 2iP). On the other hand, hyperhydricity percentage at decreased (0.4%) and at increased (0.8%) levels of agar were 72.37% and 39.08%, respectively. MS medium modification with NH4NO3 (412 mg/l), KNO3 (475 mg/l) and CaCl2.2H2O (880 mg/l) was found the best medium to reduced hyperhydricity (23.6%).

Resumo A hiperidricidade é um distúrbio fisiológico sério e afeta a propagação in vitro de muitas plantas e também da Salvia santolinifolia. O material doador para iniciar a cultura in vitro foi o calo retirado dos brotos in vitro produzidos em meio Murashig e Skoogs (MS) a 4,0 mg / l BA. Esse calo formou numerosos rebentos hiperídricos em cultura no meio da mesma composição. O objetivo foi avaliar sistematicamente o efeito das citocininas (Benziladnina (BA) e N6 - (- 2-isopentenil) adenina (2iP), magnitude dos vasos de cultura, solidificação do meio, fonte de nitrogênio e cloreto de cálcio para o alívio da hiperidricidade. culturas de tecidos de S. santolinifolia BA e 2iP induziram hiperidricidade severa, quando outros fatores, como magnitude dos vasos de cultura e uma concentração adequada de ágar, nitrato de amônio (NH4NO3), nitrato de potássio (KNO3) e cloreto de cálcio (CaCl2.2H2O), não foram otimizados. Após 30 dias de cultura, observamos 83,82% de brotos hiperídricos em níveis aumentados (1,5 mg / l 2iP) e 81,59% em níveis reduzidos (1,0 mg / l 2iP). Por outro lado, a porcentagem de hiperidricidade diminuiu (0,4%) e em níveis aumentados (0,8%) de ágar foram 72,37% e 39,08%, respectivamente. A modificação do meio MS com NH4NO3 (412 mg / l), KNO3 (475 mg / l) e CaCl2.2H2O (880 mg / l) foi encontrada melhor hiperidricidade média a reduzida (23,6%).

Range of factors in the reduction of hyperhydricity associated with in vitro shoots of Salvia santolinifolia Bioss / Gama de fatores na redução da hiperidricidade associada a brotos in vitro de Salvia santolinifolia Bioss

Hyperhydricity is a serious physiological disorder and affects In vitro propagation of many plants and as well of Salvia santolinifolia. The donor material to initiate the in vitro culture was the callus taken from the in vitro shoots produced on Murashig and Skoogs (MS) medium at 4.0 mg/l BA. This callus formed numerous hyperhydric shoots on culturing upon the medium of the same composition. The aim was to systematically evaluate the effect of cytokinins (Benzyladnine (BA) and N6-(-2-isopentenyl) adenine (2iP), culture vessels magnitude, medium solidification, source of nitrogen and calcium chloride for the alleviation of hyperhydricity. In the tissue cultures of S. santolinifolia BA and 2iP induced severe hyperhydricity, when other factors i.e. culture vessels magnitude and a suitable concentration of agar, ammonium nitrate (NH4NO3), potassium nitrate (KNO3) & calcium chloride (CaCl2.2H2O) were not optimized. After 30 days' culture, we observed 83.82% hyperhydric shoots at increased level (1.5 mg/l 2iP) and 81.59% at decreased levels (1.0 mg/l 2iP). On the other hand, hyperhydricity percentage at decreased (0.4%) and at increased (0.8%) levels of agar were 72.37% and 39.08%, respectively. MS medium modification with NH4NO3 (412 mg/l), KNO3 (475 mg/l) and CaCl2.2H2O (880 mg/l) was found the best medium to reduced hyperhydricity (23.6%).

A hiperidricidade é um distúrbio fisiológico sério e afeta a propagação in vitro de muitas plantas e também da Salvia santolinifolia. O material doador para iniciar a cultura in vitro foi o calo retirado dos brotos in vitro produzidos em meio Murashig e Skoogs (MS) a 4,0 mg / l BA. Esse calo formou numerosos rebentos hiperídricos em cultura no meio da mesma composição. O objetivo foi avaliar sistematicamente o efeito das citocininas (Benziladnina (BA) e N6 - (- 2-isopentenil) adenina (2iP), magnitude dos vasos de cultura, solidificação do meio, fonte de nitrogênio e cloreto de cálcio para o alívio da hiperidricidade. culturas de tecidos de S. santolinifolia BA e 2iP induziram hiperidricidade severa, quando outros fatores, como magnitude dos vasos de cultura e uma concentração adequada de ágar, nitrato de amônio (NH4NO3), nitrato de potássio (KNO3) e cloreto de cálcio (CaCl2.2H2O), não foram otimizados. Após 30 dias de cultura, observamos 83,82% de brotos hiperídricos em níveis aumentados (1,5 mg / l 2iP) e 81,59% em níveis reduzidos (1,0 mg / l 2iP). Por outro lado, a porcentagem de hiperidricidade diminuiu (0,4%) e em níveis aumentados (0,8%) de ágar foram 72,37% e 39,08%, respectivamente. A modificação do meio MS com NH4NO3 (412 mg / l), KNO3 (475 mg / l) e CaCl2.2H2O (880 mg / l) foi encontrada melhor hiperidricidade média a reduzida (23,6%).

Range of factors in the reduction of hyperhydricity associated with in vitro shoots of Salvia santolinifolia Bioss

Abstract Hyperhydricity is a serious physiological disorder and affects In vitro propagation of many plants and as well of Salvia santolinifolia. The donor material to initiate the in vitro culture was the callus taken from the in vitro shoots produced on Murashig and Skoogs (MS) medium at 4.0 mg/l BA. This callus formed numerous hyperhydric shoots on culturing upon the medium of the same composition. The aim was to systematically evaluate the effect of cytokinins (Benzyladnine (BA) and N6-(-2-isopentenyl) adenine (2iP), culture vessels magnitude, medium solidification, source of nitrogen and calcium chloride for the alleviation of hyperhydricity. In the tissue cultures of S. santolinifolia BA and 2iP induced severe hyperhydricity, when other factors i.e. culture vessels magnitude and a suitable concentration of agar, ammonium nitrate (NH4NO3), potassium nitrate (KNO3) & calcium chloride (CaCl2.2H2O) were not optimized. After 30 days culture, we observed 83.82% hyperhydric shoots at increased level (1.5 mg/l 2iP) and 81.59% at decreased levels (1.0 mg/l 2iP). On the other hand, hyperhydricity percentage at decreased (0.4%) and at increased (0.8%) levels of agar were 72.37% and 39.08%, respectively. MS medium modification with NH4NO3 (412 mg/l), KNO3 (475 mg/l) and CaCl2.2H2O (880 mg/l) was found the best medium to reduced hyperhydricity (23.6%).

Resumo A hiperidricidade é um distúrbio fisiológico sério e afeta a propagação in vitro de muitas plantas e também da Salvia santolinifolia. O material doador para iniciar a cultura in vitro foi o calo retirado dos brotos in vitro produzidos em meio Murashig e Skoogs (MS) a 4,0 mg / l BA. Esse calo formou numerosos rebentos hiperídricos em cultura no meio da mesma composição. O objetivo foi avaliar sistematicamente o efeito das citocininas (Benziladnina (BA) e N6 - (- 2-isopentenil) adenina (2iP), magnitude dos vasos de cultura, solidificação do meio, fonte de nitrogênio e cloreto de cálcio para o alívio da hiperidricidade. culturas de tecidos de S. santolinifolia BA e 2iP induziram hiperidricidade severa, quando outros fatores, como magnitude dos vasos de cultura e uma concentração adequada de ágar, nitrato de amônio (NH4NO3), nitrato de potássio (KNO3) e cloreto de cálcio (CaCl2.2H2O), não foram otimizados. Após 30 dias de cultura, observamos 83,82% de brotos hiperídricos em níveis aumentados (1,5 mg / l 2iP) e 81,59% em níveis reduzidos (1,0 mg / l 2iP). Por outro lado, a porcentagem de hiperidricidade diminuiu (0,4%) e em níveis aumentados (0,8%) de ágar foram 72,37% e 39,08%, respectivamente. A modificação do meio MS com NH4NO3 (412 mg / l), KNO3 (475 mg / l) e CaCl2.2H2O (880 mg / l) foi encontrada melhor hiperidricidade média a reduzida (23,6%).

Artificial pollination and use of growth regulators in atemoya 'Gefner' fruits

The objective was to study the effectiveness of the growth regulator (ANA + GA3) associated or not to the application of adjuvant and artificial pollination in 'Gefner' atemoya. The experiment was conducted in the experimental orchard at Florida's Tropical Research and Education Center. The experimental design was in a randomized block, with 14 treatments, 10 repetitions and 3 flowers per plot. The highest percentages of fixed fruits were obtained with hand pollination ­ HP and 450 NAA + 1250 GA3 mg L-1 + adjuvant and HP. The use of hand pollination for 'Gefner' atemoya tree proved to be the most efficient method so far. Applying growth regulator without artificial pollination produces parthenocarpic fruits, however with high rate of abortions, and small fruits. Growth regulators together with hand pollination produces small and uneven fruits, and cause reduction in the fruits' titratable acidity. The use of adjuvant caused low fixation and toxicity to fruits, and its use is not recommended.

Evaluación preliminar de la callogénesis y organogénesis in vitro Leptochloa crinita (lag.) p. m. peterson & n. snow / Preliminary evaluation of in vitro callogenesis and organogenesis Leptochloa crinita (Lag.) P. M. Peterson & N. Snow

RESUMEN El cambio de uso de la tierra por diversos factores socio-económicos, climáticos, tecnológicos y culturales han tenido como consecuencia en la provincia de San Luis (Argentina) la pérdida de diversidad biológica, fragmentación y destrucción de hábitat, comprometiendo la existencia de la flora nativa, como es el caso de Leptochloa crinita (Lag.) P.M. Peterson & N. Snow, una especie con buena aptitud forrajera del monte chaqueño. Bajo este contexto, el presente trabajo tuvo como objetivo aplicar biotécnicas como alternativas para una propagación apropiada de este acervo genético. Se evaluó la organogénesis y callogénesis in vitro de diferentes explantos con distintos reguladores de crecimiento en un medio nutritivo Murashige y Skoog. El tratamiento con 6 mg l-1 de 2,4-D estimuló la callogénesis, mientras que los tratamientos combinados de auxinas y citocininas presentaron las mayores tasas de morfogénesis. El desarrollo de esta biotécnica permite disponer de metodologías adecuadas para iniciar ensayos de conservación in vitro, análisis de la variabilidad genética e inicio de programas de domesticación y mejoramiento genético.

ABSTRACT In San Luis (Argentina), land-use change due to a variety of socioeconomic, climatic, technological and cultural factors has caused the loss of biological diversity, fragmentation and habitat destruction, compromising the existence of native flora, such as in the case of Leptochloa crinita (Lag.) PM Peterson & N. Snow, a species with good forage aptitude from the Chaco forest. In this context, the present work aimed to apply biotechniques as an alternative for an appropriate propagation of this gene pool. In vitro organogenesis and callogenesis of several explants were evaluated with different growth regulators on a Murashige and Skoog nutrient medium. Treatment with 6 mg l-1 of 2,4-D stimulated callogenesis, while combined auxin and cytokinin treatments presented the highest rates of morphogenesis. The development of this biotechnique makes it possible to have adequate methodologies to initiate in vitro conservation trials, analysis of genetic variability and the initiation of domestication and breeding programs.

In vitro establishment and multiplication of Echynochloa polystachya

The objective of this work was to carry out the in vitro establishment of Echynochloa polystachya aiming at obtaining a micropropagation protocol for works involving the selection of superior genotypes and the cultivation of the species. E. polystachya stems were collected in the municipality of Manaus-AM. Explants were inoculated in test tubes containing Murashige and Skoog (MS) medium. Thirty days after in vitro establishment, the rate of sprouting and contamination were evaluated. Experiments were also carried out to assess the effects of sucrose and 6-benzylaminopurine (BAP) concentrations on the tillering rate of explants. It was found that during the successive subcultures there was a decrease in internodes and the consequent loss of vigor. There were responses in the multiplication rate at concentrations starting from 45 g L-1 sucrose. In addition, BAP and sucrose interfered the development and in vitro multiplication. Sucrose in conjunction with BAP was harmful and shortened internodes. The physiological state of the explants for the species under study was intrinsically linked to the concentrations of sucrose used for the culture medium and the concentrations of BAP. However, the sucrose and BAP concentrations suggested for in vitro cultivation of E. Polystachya must be adjusted during successive subcultures. Absence of contamination in the in vitro establishment occurred at concentrations 15, 30 and 60 g L-1 sucrose. The combination of 1.5 mg L-1 BAP and 30 g L-1 sucrose promoted greater induction of sprouts. In addition, the in vitro rooting of E. polystachya was 45%.

Callus Induction, Proliferation, Enhanced Secondary Metabolites Production and Antioxidants Activity of Salvia moorcroftiana L. as Influenced by Combinations of Auxin, Cytokinin and Melatonin

Abstract: Tissue culture technique is one of the best methods to reproduce salvia plant Therefore, the aim of this research was to enhance the in-vitro callus proliferation and production of secondary metabolites of S. moorcroftiana using different combinations of auxin, cytokinin and melatonin. Initially, callus induction was optimized using indole acetic acid (IAA), 2, 4-dichlorophenoxy acetic acid (2,4-D), and naphthalene acetic acid (NAA) applied at different concentrations in combination with 1 mg L-1 of 6-benzylaminopurine (BAP). The results indicates that earliest days to callus induction (14.67 days) was occurred in the media fortified with 2, 4-D+BAP (2.0+1.0 mgL-1). Whereas the highest callus initiation (100%) was induced on MS medium incorporated with 2,4-D+BAP (1+1mgL-1). Furthermore, maximum fresh weight was obtained when 2,4- D + BAP at the rate of (1+ 1mg L-1) was incorporated and dry weight was attained when 2,4- D + BAP at the rate of (2+1 mg L-1) was added to MS media. The maximum fresh and dry weight was obtained when melatonin at rate of 1.5 mg L-1 was supplemented with MS media including 2,4-D + BAP (1+1mg L-1), moreover the maximum DPPH scavenging activity, total phenolic and flavonoid content was noted when supplemented with melatonin at rate of 1.5 mg L-1. In conclusion, among various concentrations of plant growth regulators, 2,4- D + BAP at the rate of (1+ 1mg L-1) along with 1.5 g L-1 melatonin was the best for callus growth and production of secondary metabolites of S. moorcroftiana.

Organogenesis on apical buds in common fig (Ficus carica) var. Black Jack

BACKGROUND Plant tissue culture involves the use of explants obtained from plants to induce organogenesis with the help of plant growth regulators (PGRs). Micropropagation techniques provide a faster and economical solution to the limitations associated with traditional methods of plant cultivation. The present study focuses on the multiple shoot induction and proliferation of Ficus carica var. Black Jack. Factors that influence the growth of in vitro multiple shoots on the apical buds, which include growth media and PGRs, were investigated in this study. Different concentrations of cytokinins like 6-benzylaminopurine (BAP), Thidiazuron (TDZ), and Kinetin (Kin) were used on woody plant medium (WPM) for the optimization of media for multiple shoot induction and proliferation. RESULTS Apical buds of Ficus carica var. Black Jack growing in WPM supplemented with BAP produced the healthiest plantlets, with the highest number of multiple shoots. The most efficient medium composition which produced the highest number of multiple shoots (37.8) per growing explant was WPM supplemented with 20 mM BAP. Proliferated multiple shoots were efficiently rooted using WPM + 20 mM BAP + 8 mM indole-3-acetic acid (IAA). This optimized medium composition significantly enhanced the production of multiple, disease-free plantlets using single apical bud explants of Ficus carica var. Black Jack. CONCLUSIONS In the present study the observations indicate that WPM supplemented with 20 mM BAP is the best-suited medium for organogenesis and multiple shoot culture of Ficus carica var. Black Jack, and this technique can be potentially applied for commercialization of the plant

Optimization of callus cultures at Echinacea purpurea L. for the amount of caffeic acid derivatives

BACKGROUND: In order to produce an effective callus in Echinacea purpurea L.; determination of the explant type and growth regulators that best respond to callus induction and the optimization of the culture conditions to increase the amount of caffeic acid derivatives (CADs) in the obtained callus. CADs contents of callus cultures of E. purpurea were evaluated by establishing an effective callus induction system in vitro. RESULTS: Various medium containing different growth regulators were tested using leaf, petiole, cotyledon and root as the explants. The best callus development was achieved in MS medium with 1.0 mg l 1 2,4- D + 2.0 mg l 1 BAP in leaf, 1.0 mg l 1 NAA + 0.5 mg l 1 TDZ in petiole, 2.0 mg l 1 NAA + 1.0 mg l 1 TDZ in cotyledon and 0.5 mg l 1 NAA + 0.5 mg l 1 BAP in roots. Upon optimisation of callus growth, each type of explant was cultured for 4, 6, 8 and 10 weeks in medium for the analyses of caftaric acid, chlorogenic acid, caffeic acid and chicoric acid contents. The highest amounts of caftaric acid (4.11 mg/g) and chicoric acid (57.89 mg/g) were found from petiole explants and chlorogenic acid (8.83 mg/g) from root explants at the end of the 10-week culture time. CONCLUSIONS: As a result of the present study, the production of caffeic acid derivatives was performed by providing the optimization of E. purpurea L. callus cultures. Effective and repeatable protocols established in this study may offer help for further studies investigating the production of caffeic acid derivatives in vitro.

Application methods of biostimulants affect the production and postharvest conservation of yellow melon

Worldwide, Brazil holds the fifth position in melon fruits exportation, further expanding its products to provide for the growing demand. This expansion is the result of the development and application of new technologies, including the management of the use of biostimulants. However, for melon crops, the information in the literature on the use of biostimulants remains limited to the effects of different doses on fruit quality at the time of harvest. Therefore, this study aimed to evaluate the influence of different methods of pre-harvest application of two biostimulants on the production and postharvest conservation of fruits of yellow melon cv. Iracema. The treatments consisted of a combination of three factors: two plant biostimulants (Crop Set® and Spray Dunger®), two application methods of the products (fertigation and spraying), and five times of postharvest storage (0, 14, 21, 28 and 35 days). An additional control treatment corresponded to plants without biostimulant application. The fruits were evaluated for production and physicochemical attributes: average mass, yield, flesh firmness, titratable acidity, soluble solids content, SSC/TA ratio, pH, total soluble sugars, and weight loss. Fertigation is the recommended application method of biostimulants for yellow melon due to its effect on the increase of average mass, yield, flesh firmness, soluble solids content, and total soluble sugars of the fruits in relation to the spraying method.

Effects of biopesticides in Tetragonisca angustula latreille (Hymenoptera: Meliponinae) pollinators / Efeitos de biopesticidas em polinizadores Tetragonisca angustula latreille (Hymenoptera: Meliponinae) / Efectos de los bioplaguicidas sobre los polinizadores Tetragonisca angustula latreille (Hymenoptera: Meliponinae)

Stingless bees Tetragonisca angustula (Latreille) (Hymenoptera: Meliponinae) are pollinators of native and cultivated plants and are therefore in contact with areas contaminated by pesticides. These native bees were evaluated for changes in gene expression of esterase isoenzymes (EST) and peptides after contamination by contact with growth regulators from insecticides Gallaxy® EC 100, Natuneem and Azamax after 48, 120, 168 hours, 30 and 60 days. EST-4 presented an increase in relative activity after contamination with Gallaxy® EC 100 at 6.2 × 10-2 g a.i./mL; Natuneem at 7.5 × 10-5 g a.i./mL; and Azamax at 1.2 × 10-3 g a.i/mL after 60 days, 48 h, and 60 days, respectively. Inhibition of the relative activity of EST-4 was detected after contamination by Natuneem at 1.5 × 10-5 g a.i./mL and Azamax at 1.2 × 10-3 g a.i./mL after 48 h and 30 days, respectively. The insecticide growth regulators promoted changes in protein synthesis of T. angustula adult workers resulting in an increase or decrease in the relative intensity of bands, and the appearance of new peptides when compared with controls. Changes in protein synthesis have been identified mainly after long period of contamination, 120 and 168 h with the IGRs Gallaxy® EC 100 (at 0.78 and 1.25 g a.i./mL), Azamax (at 1.2 × 10-3 and 6 × 10-3 g a.i./mL), and Natuneem (at 7.5 × 10-5 and 3 × 10-3 g a.i./mL), and at 60 days with Natuneem (at 1.5 × 10-5 g a.i./mL).(AU)

Abelhas sem ferrão Tetragonisca angustula (Latreille) (Hymenoptera: Meliponinae) são polinizadores de plantas nativas e cultivadas e, portanto, estão em contato com áreas contaminadas por biopesticidas. Essas abelhas nativas foram avaliadas quanto a alterações na expressão gênica de isoenzimas esterases (EST) e peptídeos após contaminação por contato com reguladores de crescimento de inseticidas Gallaxy® EC 100, Natuneem e Azamax após 48, 120, 168 horas, 30 e 60 dias. A EST-4 apresentou um aumento na atividade relativa após a contaminação com Gallaxy® 100 EC em 6,2 × 10-2 g i.a./mL, Natuneem em 7,5 × 10-5 g i.a./mL e Azamax em 1,2 × 10-3 g i.a./mL após 60 dias, 48 h e 60 dias, respectivamente. A inibição da atividade relativa de EST-4 foi detectada após contaminação pelo Natuneem a 1,5 × 10-5 g i.a./mL e Azamax a 1,2 × 10-3 g i.a./mL após 48 he 30 dias, respectivamente. Os reguladores de crescimento de inseticidas promoveram alterações na síntese protéica de trabalhadores adultos de T. angustula, resultando em um aumento ou diminuição da intensidade relativa das bandas e no aparecimento de novos peptídeos em comparação com os controles. Alterações na síntese de proteínas foram identificadas principalmente após um longo período de contaminação, 120 e 168 h com o IGRs Gallaxy® EC 100 (0,78 e 1,25 g i.a./mL), Azamax (1,2 × 10-3 e 6 × 10-3 g i.a./mL) e Natuneem (7,5 × 10-5 e 3 × 10-3 g i.a./mL) e 60 dias com Natuneem (1,5 × 10-5 g i.a./mL).(AU)

Las abejas sin aguijón Tetragonisca angustula (Latreille) (Hymenoptera: Meliponinae) son polinizadores de plantas nativas y cultivadas y, por lo tanto, están en contacto con áreas contaminadas por bioplaguicidas. Estas abejas nativas fueron evaluadas para detectar cambios en la expresión génica de isoenzimas esterasa (EST) y péptidos después de la contaminación por contacto con los reguladores del crecimiento insecticidas Gallaxy® EC 100, Natuneem y Azamax después de 48, 120, 168 horas, 30 y 60 días. EST-4 mostró un aumento en la actividad relativa después de la contaminación con Gallaxy® 100 EC a 6.2 × 10-2 g i.a./mL, Natuneem a 7.5 × 10-5 g i.a./mL y Azamax a 1.2 × 10-3 g i.a./mL después de 60 días, 48 hy 60 días, respectivamente. La inhibición de la actividad relativa de EST-4 se detectó después de la contaminación por Natuneem a 1.5 × 10-5 g i.a./mL y Azamax a 1.2 × 10-3 g i.a./mL después de 48 hy 30 días. respectivamente. Los insecticidas reguladores del crecimiento promovieron cambios en la síntesis de proteínas de trabajadores adultos de T. angustula, resultando en un aumento o disminución de la intensidad relativa de las bandas y en la aparición de nuevos péptidos en relación a los controles. Los cambios en la síntesis de proteínas se identificaron principalmente después de un largo período de contaminación, 120 y 168 h con IGRs Gallaxy® EC 100 (0.78 y 1.25 g i.a./mL), Azamax (1.2 × 10-3 y 6 × 10-3 g i.a./mL) y Natuneem (7.5 × 10-5 y 3 × 10-3 g i.a./mL) y 60 días con Natuneem (1.5 × 10-5 g i.a./mL).(AU)