Plasmid profiles and antibiotic resistance of Pseudomonas aeruginosa isolated from different clinical samples in Jordan

Aims@#The aim of this study was to investigate plasmid profiles and antibiotic resistance patterns of multidrug resistant Pseudomonas aeruginosa strains isolated from different clinical specimens in Jordan.@*Methodology and results@#Pseudomonas aeruginosa strains were isolated from different clinical specimens from different hospitals and primary health care centers. The antimicrobial susceptibility of P. aeruginosa isolates was determined using the disc diffusion method against 16 commonly used antimicrobial drugs. Plasmid DNAs were extracted from lysed P. aeruginosa cells using the plasmid alkaline lysis method and visualized using agarose gel electrophoresis followed by ethidium bromide staining. The isolated strains of P. aeruginosa were resistant to cefepime (90%), meropenem (70%), ceftazidime (60%), piperacillin (55%), aztreonam (50%), ciprofloxacin and tobramycin (35%), gentamicin (29%), imipenem and amikacin (20%). All the isolates were sensitive to colistin. Plasmid analysis of the clinical isolates showed the presence of 0 to 3 plasmids with a size range of 1 to 25 kb compared to the standard strain of Escherichia coli (ATCC 25922).@*Conclusion, significance and impact of study: @#The results obtained in this study showed some correlation between the patterns of antibiotic resistance and plasmid profiles.

Analysis of drug resistance and pathogenicity of six strains of Klebsiella pneumoniae / 安徽医科大学学报

Objective @#To investigate the drug resistance and pathogenicity of six clinical isolates of Klebsiella pneu- moniae (Kp) ，and to provide a basis for prevention and treatment of Kp infection．@*Methods @#The six strains from different hospitals were isolated ，cultured ，and identified by species-specific gene khe． Their whole genome se- quences (WGS) were obtained using next-generation sequencing technology (NGS) ．Based on the WGS，the cap- sular serotypes，sequence types (ST) and drug-resistance genes of six strains were identified．The capsular sero- type genes and virulence genes were validated or identified using PCR. Broth microdilution tests were conducted to validate their drug susceptibility，and mice were challenged with Kp aerosols by MicroSprayer aerosolizer to evaluate their pathogenicity. @*Results @# The six strains were all serotype K2 but belonged to four ST types ( ST14 ，ST65， ST700，and ST86) ，and collectively carried six virulence genes and 23 drug-resistance genes．All the six strains were resistant to ampicillin，but only one strain was multidrug-resistant．Four strains exhibited high mucoid charac- teristics．Five strains could cause mortality in mice，which were preliminary identified as high virulence strains. @*Conclusion @# For the six Kp clinical isolates from different sources，only one strain named NY 13294 is both multi- drug-resistant and highly virulent，and other four highly virulent strains are resistant to one or two types of antibiot- ics.

Isolation and characterisation of Leishmania (Leishmania) infantum from cutaneous leishmaniasis patients in northeast Brazil

BACKGROUND In Brazil, Leishmania (Leishmania) infantum is a widely distributed protozoan parasite. The human leishmaniasis caused by this species is often associated with visceral form. Tegumentary leishmaniasis (TL) cases due to L. (L.) infantum in the country are considered rare but may be underestimated. Although probably uncommon, these cases represent a new challenge to the prevention and control of leishmaniasis. OBJECTIVES Here, we describe two distinct cases of TL with atypical clinical presentations caused by L. (L.) infantum. METHODS AND FINDINGS Parasites were isolated from cutaneous lesions of the two patients and typed as L. (L.) infantum after sequencing of the ribosomal DNA internal transcribed spacer. The dermotropic L. (L.) infantum isolates were compared in terms of growth culture patterns, metacyclogenesis and in vitro infectivity in macrophages. MAIN CONCLUSIONS This study addresses the emergence of L. (L.) infantum as a causative agent of cutaneous disease in a visceral leishmaniasis hotspot located in northeast Brazil. The data presented provides novel information about the presence of dermotropic L. (L.) infantum in the country and demonstrates the infectivity potential of theses isolates.

Incidence of Fusarium oxysporum spp. Udum on Pigeonpea in Bundelkhand Region, India

Survey was conducted of pigeonpea growing areas of 4 districts in Bundelkhand region of Uttar Pradesh during 2019-20. Fusarium udum was found constantly associated with the root samples. This indicates that this fungus, a well-known wilt pathogen, was primarily responsible for the wilt disease of pigeonpea. The average disease incidence ranged between 3.25% to 49.00% from district to districts. The average incidence percentage of wilted plants in Chitrakoot district was 39.06% followed by Banda district 25.67%, Hamirpur district 15.99% and Mahoba district 14.64% respectively. The maximum wilt (Fusarium oxysporum) incidences were recorded in Chitrakoot district followed by Banda, Hamirpur, and Mahoba. Chitrakoot isolate of F. udum was found more pathogenic and caused higher wilt incidence than other isolate. All the isolates differed in their radial growth colony characters on both solid media. It was found that PDA was the best medium in compare to PSA. The Chitrakoot isolate and radial growth was fast growing followed by others. Sporulation was moderate to excellent in different isolates. However, the maximum radial growth was a recorded-on PSA in Chitrakoot isolate and minimum radial growth in Mahoba isolate. The most distinguishing characteristic of the macro conidia are their strongly curved or hooked apices and measure 11-21.12 x 1.95 to 3.78µm.

Efficacy of Different Fungicides and Trichoderma Isolates against Leaf Blight Disease of Piper longum, Tylophora indica and Hibiscus subdariffa under in vitro

Leaf blight of the medicinal plants (Piper longum, Tylophora indica and Hibiscus subdariffa) is one of the most significant diseases that severely damage these crops throughout the year in West Bengal .So, management of this disease is essential at now. A few chemical fungicides and trichoderma isolates were tested under in vitro against the disease-associated pathogens in order to identify efficient chemical fungicides and bio agents.It was found that the trichoderma isolates T-2 and T-3 were effective against Colletotrichum gloeosporioides, whereas the isolates T-1 and T-2 were efficient in inhibiting the growth of the test pathogen better against Fusarium sp. and Sclerotium rolfsii.With a higher percentage of inhibition (77.60%), copper oxychloride was the most effective fungicide against Fusarium sp. Conversely, carbendazim and dithane m-45 were more effective against Sclerotium rolfsii and Colletotrichum gloeosporioides, showing 78.33% and 77.93% of inhibition, respectively, and less effective against Fusarium sp at higher concentrations (400 ppm). To determine whether or not these chemical fungicides and trichoderma isolates are effective under in vivo, they will be tested in the field against the corresponding pathogens associated with the disease.

Enzymase Activity of Gut Microbes Isolated from the Rugose Spiraling Whitefly Aleurodicus rugioperculatus

This study investigated the enzymatic diversity among various bacterial isolates of Rugose Spiraling Whitefly (Aleurodicus rugioperculatus), shedding light on their potential industrial applications. Notably, Bacillus cereus (MRSW01) exhibited remarkable amylase and lipase activities, aligning with its reputation as a prominent amylase producer. These enzymes are crucial in the food industry, particularly in starch and lipid degradation processes. Pseudomonas helleri (CHRSW028) demonstrated substantial cellulase activity, essential for breaking down cellulose in plant materials, suggesting its relevance in biofuel production and waste management. Pseudomonas psychrophila (CRSW024) stood out with the highest pectinase and significant lipase activities, both of which find applications in the food and textile industries and the hydrolysis of fats and oils, respectively. Proteus vulgaris strains MRSW05 and CHRSW02 displayed significant amylase, cellulase, and protease activities, making them versatile candidates for various industrial applications, including detergent formulations and bioremediation processes. Conversely, Hafnia paralvei exhibited comparatively lower enzymatic activities, indicating limited industrial potential. The rich enzymatic diversity within these bacterial isolates and highlight their potential contributions to various industrial sectors, ranging from food and textiles to biofuel production and waste management. Such insights can inform the development of biotechnological applications and processes, ultimately benefitting both industrial and environmental domains.

Cultural and Morphological Variabilty in Rhizoctonia solani Isolates of Different Rice Growing Areas of Chhattisgarh, India

The sheath blight disease of rice caused by Rhizoctonia solani is an economically important disease in India. A survey was carried out to find the disease severity of sheath blight of rice and collected the disease samples from the different locations of Chhattisgarh. Fifty eight isolates of R. solani collected from various locations of Chhattisgarh were studied for their morphological and cultural variation. Cultural and morphological parameters of each isolate like growth pattern of mycelium, colony colour, Colony growth diameter (mm) at different intervals was recorded after inoculation of R. solani on to the sterilized PDA in 90 mm Petri plates incubated at 28 ± 2 0C in B.O.D incubator after 15 days [1]. The isolates were assigned code numbers such as RS1, where "RS" named Rhizoctonia solani and "1" denote the serial number of the isolate. The R. solani isolates RS1, RS2, RS3, RS4, RS5, RS12, RS19, RS34, RS50 and RS57 were recorded as fast growing isolates. The color of the fungal colony was varied from light brown to dark brown.

Protein Quality Characterization of Defatted White Sesame Cake and Its Protein Isolate

Present work evaluated the protein quality of defatted white sesame flour and the protein isolates obtained from sesame cake through the alkaline extraction at a pH 9.5. The study was conducted at Department of Foods and Nutrition, Post Graduate and Research Center (PGRC), College of Community Science and MFPI - Quality Control Laboratory, Professor Jayashankar Telangana State Agricultural University, Rajendranagar, Hyderabad (India) during 2021-2023. Defatted white sesame cake was subjected to alkaline extraction at pH 9.5 and resultant isolates were evaluated for nutrient composition, scanning electron microscope imaging, amino acid composition and sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) analysis. The obtained data was subjected to one-way analysis of variance. The white sesame protein isolates had a protein content of 93.83%, isolate recovery of 37.00 g/100 g and protein yield of 71.77%. The non-essential amino acids (NEAAs) content of the defatted white sesame flour and white sesame protein isolates ranged between 68.06% to 70.60% of the total protein content, while 29.40% to 31.94% was essential amino acids (EAAs). Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) analysis of defatted white sesame flour WSF and white sesame protein isolate (WSPI) exhibited protein bands within the molecular weight range of 20 to 62 kDa. The protein isolates derived from sesame seed cake has promising potential for integration into diverse food formulations, offering an avenue to combat protein deficiencies.

Qualified Studies on the Pathogenic Variability of Alternaria brassicae, the Causal Agent of Blight Disease of Rapeseed-Mustard

Alternaria blight of mustard caused by Alternaria brassicae is one of the major disease which causes an economic yield losses in Uttar Pradesh. Total ten (10) isolates of Alternaria brassicae were collected from different host from different states of India and characterized for pathogenic variations. The incubation period was maximum in Brassica carinata (8.82) followed by B. napus (8.70), B. nigra (7.86), B. juncea (6.00), while it was minimum in B. campestris var. yellow sarson (5.83) for all the isolates. The number of spots per leaf recorded maximum on Brassica juncea (17.66) followed by B. napus (12.66), B. nigra (9.66), B. campestris var. yellow sarson (9.00), while it was minimum in B. carinata (7.66) at 80 days after sowing (DAS). The size of spots per leaf recorded maximum on Brassica juncea (14.89 mm) followed by B. campestris var. yellow sarson (12.48 mm), B. nigra (7.89 mm), B. napus (6.96 mm), while minimum in B. carinata (5.12 mm) at 80 DAS. The number of spots on pods recorded maximum on Brassica juncea (14.66) followed by B. campestris var. yellow sarson (12.66), B. nigra (10.66), B. napus (7.66), and B. carinata (6.33) at 105 DAS. The results revealed that maximum PDI was noted on Brassica juncea (66.70) followed by B. campestris var. yellow sarson (62.99), B. nigra (60.25), B. napus (29.85), while minimum in B. carinata (24.42).

Effect of Brine Concentration on Bacteria Isolated from Leafy Vegetables (Talinum triangulare, Telfairia occidentalis and Vernonia amygdalina)

The effect of brine on bacteria isolated from leafy vegetables was evaluated. Fresh waterleaf (Talinum triangulare), Pumpkin leaves (Telfairia occidentalis) and Bitter leaves (Vernonia amygdalina) including cooking salt were bought from vendors in Mile 3 market, Port Harcourt, Rivers State. Brine concentrations of 1, 2, 3, 4 and 5% were prepared by dissolving appropriate grams of salt in distilled water. Nine millilitres of the respective concentrations were transferred into clean test tubes, labelled, stoppered with foil and autoclaved at 121? for 15 minutes at 15psi. Sterile distilled water served as a control. The test isolates were standardized based on 0.5McFarland and 1mL each was introduced into different brine concentrations. The standard plate count was used to monitor brine effects on isolates and this was done hourly for six hours. Inoculated plates were incubated at 37? for 24 hours. After incubation, enumerated colonies were used to deduce isolate mortality. The total heterotrophic bacterial (THB) load of bitter, Pumpkin and water leaves were 3.49±0.4×106, 3.25±0.4×106 and 1.99±0.2×106 CFU/g, respectively. The staphylococcal counts for bitter, Pumpkin and water leaves were 1.65±0.3×104, 3.13±0.5×104 and 1.55±0.4×104 CFU/g, respectively. Total coliform counts for bitter, Pumpkin and water leaves were 1.52±0.8×105, 2.85±0.1×105 and 1.75±0.6×105 CFU/g, respectively. Staphylococcal counts of pumpkin leaves were significantly (P?0.05) higher than those obtained for bitter leaf and water leaf. There was no significant difference(P>0.05) in the THB and Coliform counts of all samples. E. coli was predominant in Pumpkin and water leaves while Staphylococcus sp was predominant in bitter leaves. The LC50 values for E. coli, Klebsiella, Staphylococcus, and Bacillus sp were; 5.39, 3.88, 1.62, and -0.41mg/ml, respectively. The LC50 showed that the brine was very lethal on Bacillus sp and Staphylococcus sp. High brine concentration is recommended to achieve reduced bacterial load.

Bacteriological Assessment of Critical Areas in Three Selected Units in Jodhpur Dental College General Hospital, Jodhpur Rajasthan, India

The hospital environment plays a crucial role in the chain of infection spread. Thus, there is need to attack the chain of infection at its weakest link as the most effective way in combating and/or preventing nosocomial infections. The study aims to assess the common aerobic pathogenic bacteria in the different departments of Jodhpur National University Dental Hospital and to establish the best disinfectant as well as disinfection procedure. A cross sectional descriptive study was conducted. Air and surface samples were taken before and after dental procedures from all the three departments within durations of 14 days. Different antiseptics were used so as to compare their effectiveness. Swabs taken from different places were streaked on blood agar plates and incubated at 37oC under aerobic conditions for 24 hours. After incubation, isolates obtained were appropriately identified. After aerobic incubation of the settle plates at 370C for 24hours, the colonies on each plate were counted and recorded as the number of bacteria carrying particles settling over the area of the plate in a given period of time. The level of bacterial contamination of air is usually expressed as the number of bacteria carrying particles per cubic millimeter. A total of 274 surface samples and 97 air samples were collected. Bacteria were isolated in all air samples while only 255 surface samples had growth. The predominant species in all services was Bacillus spp, followed by coagulase negative Staphylococci, Micrococcus luteus, aerobic spore formers and least was Pseudomonas aeruginosa. The bacteria isolated in the air were similar to those isolated from surfaces. In conclusion, lack of a universal procedure for surveillance of nosocomial infection, presence of pathogenic bacteria, poor hand hygiene and heavy contamination of some important surfaces are the most important problems in our hospitals.

ANTIMICROBIAL SUSCEPTIBILITY PATTERN OF UROPATHOGENS AT A TERTIARY CARE HOSPITAL IN CENTRAL INDIA DURING COVID ERA.

Objective: The purpose of this study was to study the bacteriological profile of UTI in patients attending the tertiary care hospital and to study the antimicrobial sensitivity pattern of uropathogens.Methods: This cross-sectional study was conducted after obtaining clearance from the institutional ethics committee. Clean-catch mid-stream urine samples were collected from patients symptomatic of UTIs. Samples were cultured aerobically on CLED agar. Isolates having significant growth (>105CFU/ml) were further processed for identification using standard microbiological techniques and their antimicrobial susceptibility pattern was evaluated by the Standard Kirby Bauer disk diffusion method as per CLSI 2020 guidelines.Results: A total of 480 urine samples were processed, yielding 174 isolates. Escherichia coli (42.50%) was predominant, followed by Klebsiella pneumonia, Pseudomonas aeruginosa, Staphylococcus aureus, Acinetobacter spp., Proteus spp., Providencia spp., Enterococcus spp., Citrobacter spp. and Morganella morganii. Gram-positive isolates exhibited high sensitivity towards vancomycin, linezolid, meropenem, and piperacillin tazobactum. Enteric coliforms exhibited high sensitivity towards colistin, meropenem, aminoglycosides, and piperacillin tazobactum. Non-fermenters exhibited high sensitivity towards colistin, meropenem, cefepime, and amoxycillin clavulanate.Conclusion: The rampant injudicious irrational overuse of antibiotics has led to the emergence of multi-drug resistant bugs, which is posing a serious challenge to clinicians in the management of infections. Developing therapeutic protocols guided by susceptibility profiles for tuning antibiotic therapy regimens is an important strategy in tackling this menace.

Study of prevalence and antimicrobial susceptibility pattern of organisms isolated from various clinical specimen of the patients admitted in intensive care unit of tertiary care hospital

Background: Mostly intensive care unit (ICU) patients are more susceptible to nosocomial infections caused by hospital-based various strains of bacteria and other opportunistic pathogens. Due to the widespread use of broad-spectrum antibiotics, these strains of pathogens are often multi-drug resistant. To prevent resistance against the antimicrobial agent various departments of the health care system have to work together, so we can use the antimicrobial agents as effectively as we can to treat illnesses. Aim and Objectives: The objective of this study was to know the prevalence of different micro-organisms causing infections in ICU and their sensitivity and resistance pattern and to determine the overall microbiological and resistance profile which helps formulate therapeutic guidelines in ICU. Materials and Methods: A cross-sectional study was conducted at a tertiary care teaching hospital in Ahmedabad to assess the culture and sensitivity pattern of clinical samples such as blood, urine, sputum, wound, and endotracheal aspiration for a 1-year duration (August 2019 - August 2020). Results: A total of 941 samples were received for microbiological investigation from ICU, out of which 322 were positive. The Utmost isolated organism was - Klebsiella (37.26%) followed by Escherichia coli (16.45%), Pseudomonas (12.42%), and Staphylococcus aureus (7.45%). The Gram-negative bacteria (GNB) were most sensitive to drugs like colistin (96.26% %) and tigecycline (83.40%) followed by carbapenems (71.79%), aminoglycosides (71.36%), and fluoroquinolones (67.21%). More sensitive drugs for isolated Gram-positive organisms were linezolid (100%) followed by teicoplanin (98.41%) and vancomycin (98.41%). Conclusion: High prevalence of multidrug-resistant organisms such as methicillin-resistant S. aureus, vancomycin-resistant enterococci and GNB producing Extended-spectrum Beta-lactamase, AmpC, or carbapenem-resistant GNB in our study, raise serious concerns about antibiotic resistance. The main reason for increasing antimicrobial-resistant bacteria is poor infection control practices and inappropriate use of antibiotics. Hence, research regarding antibiotic sensitivity and resistance will be very helpful for doctors to initiate appropriate empirical antibiotics in treating critical illnesses.

Whole genomic features analysis of coxsackievirus B3 strains isolated in Tianjin / 中国热带医学

@#Abstract: Objective　To genotype and analyze whole genomic features of Coxsackievirus B3 (CVB3) isolated in Tianjin, to improve evolution information of CVB3 virus in Tianjin, and to provide basis for surveillance and early warning of related diseases. Methods　Viral RNA was extracted from five CVB3 strains isolated in Tianjin, whole genome sequence of the virus was amplified by RT-PCR and sequenced by next-generation sequencing method, and phylogenetic and recombinant analysis were carried out. Results　The open reading frame 1(ORF) of the five CVB3 strains contained 6 555 nucleotides and encoded 2 185 amino acids, and ORF2 was composed of sequences encoding 68 amino acids. The nucleotide sequence similarity ranged from 78.3%-100%, and the amino acid sequence similarity ranged from 95.7%-100%. Compared with the CVB3 prototype strain, the nucleotide sequence similarity of the five viruses was between 78.2%-79.1%, and the similarity of amino acid sequences was 94.9%-95.3%. All five viruses exhibited a T151A mutation on the VP2 protein. Additionally, the encephalitis isolate showed a K158E mutation on the VP2 protein, while one of the sewage isolates had a C234T mutation in 5' noncoding region. The five strains belonged to two different genotypes, among which the encephalitis isolate in 2016 belonged to the D genotype, while the sewage isolates in 2021 belonged to the E genotype. This is also the first report of E genotype CVB3 in northern China. The CVB3 strain may have recombinant events in non-structural protein regions, in which encephalitis isolate may recombine with a Coxsackievirus B5 (CVB5) strain, while sewage isolates may have recombinant events with a strain of ECHO virus 18 (E18). Conclusions　The CVB3 isolates in Tianjin belong to D and E genotypes, and recombination events may exist in non-structural protein region of the viral genome. The results of CVB3 virus genome analysis in sewage suggests presence of CVB3 infection in the population of Tianjin, and its epidemic dominant genotype may have changed.

Streptococcus mutans and Streptococcus dentisani in dental biofilm of children with different caries status: a pilot study / Streptococcus dentisani e Streptococcus mutans no biofilme dental de crianças com diferentes estados de cárie: um estudo piloto

Objective:Streptococcus mutans is one of the etiological agents associated with caries due to its ability to metabolize carbohydrates and resist acidic environments. On the other hand, Streptococcus dentisani, shows characteristics associated with caries control due to its ability to inhibit growth of cariogenic bacteria. The aim of this work was to quantify the levels of Streptococcus mutans and Streptococcus dentisani from dental biofilm of children related to their caries situation. Material and Methods: After identification of morphologic characteristics of reference strains was performed, clinical isolates of biofilm compatible with these strains were selected and the Polymerase Chain Reaction technique was performed using species-specific primers. Biofilm samples from 25 children with caries and 21 without caries were collected to quantify the levels of S. mutans and S. dentisani. Results: There were statistically significant differences in the levels of S. mutans in the caries group and the levels increased as the severity of the carious lesion increased. By contrast, higher levels of S. dentisaniwere found in the caries-free group, although no statistically significant differences were found. In addition, the levels of S. dentisani decreased as the severity of the carious lesion increased. Conclusion: The increase in the frequency of S. dentisani in the caries-free group suggests the possibility of requiring minimum levels of this species in the dental biofilm to show an actual protective effect. It must also be considered that this effect might be related to intrinsic factors in children and the intraspecies genetic variability found in every individual. (AU)

Objetivo : Streptococcus mutans é um dos agentes etiológicos associados à cárie devido à sua habilidade de metabolizar carboidratos e resistir a ambientes ácidos. Já o Streptococcus dentisani , apresenta características associadas ao controle da cárie devido à sua capacidade de inibir o crescimento de bactérias cariogênicas. O objetivo deste trabalho foi quantificar os níveis de Streptococcus mutans e Streptococcus dentisani no biofilme dental de crianças em relação à situação de cárie destas. Material e Métodos: Após a identificação das características morfológicas das cepas de referência, foram selecionados do biofilme isolados clínicos compatíveis com essas cepas e realizada a técnica de Reação em Cadeia da Polimerase utilizando primers espécie-específicos. Amostras de biofilme de 25 crianças com cárie e 21 sem cárie foram coletadas para quantificar os níveis de S. mutans e S. dentisani. Resultados: Houve diferenças estatisticamente significativas nos níveis de S. mutans no grupo com cárie e os níveis aumentaram à medida que a gravidade da lesão cariosa aumentou. Por outro lado, foram encontrados níveis mais elevados de S. dentisani no grupo sem cáries, embora não tenham sido encontradas diferenças estatisticamente significativas. Além disso, os níveis de S. dentisani diminuíram à medida que a gravidade da lesão cariosa aumentava. Conclusão: O aumento da frequência de S. dentisani no grupo livre de cárie sugere a possibilidade de exigir níveis mínimos desta espécie no biofilme dental para mostrar um efeito protetor real. Deve-se considerar também que esse efeito pode estar relacionado a fatores intrínsecos nas crianças e à variabilidade genética intraespécie encontrada em cada indivíduo. (AU)

Screening of lysine production by Escherichia coli and Klebsiella isolates at different carbon sources

Lysine is an essential amino acid that is not biologically manufactured in the body. Different chemical methods for lysine production are expensive and give low yields. The present study was conducted with the purpose to evaluate the biochemical production of lysine by different carbon sources using bacterial isolates. Three carbon sources namely glucose, sucrose, and fructose were used to evaluate the biochemical production of lysine by Escherichia coli and Klebsiella spp. isolates. Optimum incubation periods were between 48-96 hours. An extensive amount of lysine was produced by all of these isolates in L6 fermentation medium. Maximum lysine was produced by Klebsiella isolate K1 6.48 g/L after 96 hours of incubation by using glucose as carbon source followed by 6.0 g/L by Klebsiella isolates K3 after 72 hours of incubation when sucrose was used as a carbon source at 37 °C. Highest amount of lysine was produced at 96 hours by Klebsiella isolates in addition to E. coli. From all three carbon sources using Klebsiella isolates and E. coli, glucose showed better lysine production.

Characterization of Virulent Bacterial Isolates Associated with Multi-Drug Resistance among Patients with Surgical Site Infections in Selected Specialist Hospitals in Calabar, Nigeria

Aims: Increasing research findings have documented the continuous emergence and threats posed by drug resistant clinical isolates from post-operative wound infections to commonly used antibiotics globally. This hospital-based study investigated virulent bacterial pathogens implicated with post-operative wound infections among surgical site infection (SSI) patients in Calabar, Nigeria and determined their antibiotic resistance pattern. Methodology: A total of 127 bacterial isolates of different genus from 110 SSI patients, were isolated from pus and surgical wound exudates and fully characterized using standard bacteriological procedures. Antimicrobial susceptibility patterns of isolates were determined using Kirby- Bauer disk diffusion method, following the guidelines by Clinical Laboratory Standard Institute (CLSI). Results: Multi-drug resistant bacteria isolated and their percentage frequency were coagulase Negative Staphylococci (21.3%), Staphylococcus aureus (19.7%), Pseudomonas aeruginosa (14.2%), Escherichia coli (11.8%), Klebsiella pneumoniae (9.4%), Enterococcus faecium (6.3), Enterobacter cloacae (4.7%), Proteus mirabilis (4.7%), Acinetobacter baumannii (3.1%), Pseudomonas putida (3.1%) and Aerococcus viridans (1.6%). Among gram-positive bacteria isolated, S. aureus showed highest resistance to several antimicrobials (100% to oxacillin, 96% to ciprofloxacin, 92% to levofloxacin, and 76% resistance to vancomycin). All recovered S. aureus isolates were cefoxitin screen positive indicating possible MRSA isolates. Additionally, among Gram-negative isolates K. pneumoniae was found to possess higher resistance to several antibiotics (66.7% resistance to each of ciprofloxacin, levofloxacin, ceftazidime, trimethoprim /sulfamethoxazole, cefazolin, ampicillin, tobramycin and 58.3% resistance to each of ceftriaxone, gentamicin, and ampicillin/sulbactam). Statistical analysis of categorical variables of study subjects revealed that length of hospital stay, type of surgery, previous admission history, antibiotic use, and age were significant (p<0.05) in SSI outcome of patients, while patients� gender was not significant (p>0.05) in SSI outcome. Conclusion: Adherence to measures of strict infection control, optimal preoperative, intraoperative, and postoperative patient care, including multifaceted approaches involving surveillance, and antimicrobial stewardship, are vital to SSI treatment outcomes.

Morphological Diversity of Fungal Strains Responsible for Dessert Banana Rot, Musa Sapientum (Musaceae) Produced in the South Comoe Region on Ivory Coast

Aims: The banana dessert grown in different regions of the world, encounters important difficulties that cause it to lose its marketability especially because of the diseases related to conservation. The objective here is to identify the strains responsible for fungal diseases of bananas when it is stored. Study Design: This study was undertaken in order to ensure the competitiveness of the dessert banana from Ivory Coast on the international market which is threatened by the recurrent problem of post-harvest rot. Place and Duration of Study: Agrovalorisation Laboratory, Agroforestry Training and Research Unit, Université Jean Lorougnon GUEDE Daloa Côte d'Ivoire, between February 2021 and March 2022. Methodology: The study involved 120 bananas divided into two batches of 10 hands each, one with signs of necrosis and the other with no signs. Banana fragments (crown, epicarp and explant) were deposited on the growing media at several distinct points and slightly embedded in the agar. The resulting colonies were transplanted successively until a pure strain was obtained from a single mushroom colony per petri dish. Results: A total of 11 different genera were identified from 105 isolates. 57 are from necrotic bananas and 36 from bananas with no signs of necrosis. Fungal strains isolated there are: Trichoderma sp. (15%), Fusarium sp. (1%), Scytalidium sp. (39%), Mucor sp. (1%), Scopulariopsis sp. (1%), Alternaria sp. (4%), Aureobasidium sp. (1%), Aspergillus of the Glaucus group (10%), Cladosporium sp. (2%), Pseudallescheria sp. (6%) and Chrysosporium sp. (20%). No strains of the genus Colletotrichum musea responsible for anthracnose that can develop on both green and ripe fruit have been isolated. However, morphological characterization has not among to identify several other species (12) especially those not sporulating. Conclusion: This diversity of isolated strains in this work is identical to that most frequently isolated and cited in the literature.

Bacterial Profile Of Blood Culture And Their Anti-Microbial Susceptibility Pattern In Pediatric Patients Of A Tertiary Care Hospital

Introduction: Blood stream infection are very common in the pediatric age group and these are one of the common causes of morbidity and mortality in children. In developing countries ,the rate of blood stream infection in children is about 20-50%.The present study was undertaken to determine the etiological agents causing blood stream infection and their antibiotic susceptibility pattern in pediatric patients. Patients with bacteremia may have either a transient bacteremia or persistent bacteremia which can be self-limited without development of focal infection or sequelae or may progress to a more serious fatal infection or toxic effects. The present study in a hospital basedMethodology: single centred, Observational study, of 3 years. Blood sample were collected in BacTec bottle and standard microbiological protocol were applied for the isolation identification of bacteria strains. Antimicrobial susceptibility test was performed by the Kirby Bauer Disc Diffusion Method as per CLSI 2019 guidelines. Out of total 350 bloodResults: samples received for culture,87(23.14%)were culture positive, out of which 42/87(48.27%) were Gram positive organisms and 39/87(44.82%) were Gram-negative organisms and 6 /87(6.89%) were candida spp. The most common organism was Staphylococcus aureus(31.03%) the predominant organism followed by Klebsiella pneumoniae(21.83%) and Streptococcus pneumoniae (9.19%), Escherichia coli, Enterobacter cloacae each (5.74%). All Gram positive bacteria were susceptibile to vancomycin, teicoplanin and linezolid. 11/27(40.74% )of Staphylococcus aureus were Methicillin resistant Staphylococcus aureus (MRSA) strains. All Gram negative bacteria were susceptibility to amikacin, Colistin,Tigecycline. Staphylococcus aureus is the leading cause ofConclusion: childhood septicemia in this locale, has been decline in susceptibility of the pathogens to common antibiotics which ultimately stresses on the need for continuous screening and surveillance for antibiotic resistance in the pediatric ward and calls for increased efforts to ensure more rational use of these drugs.

Creación del primer biorrepositorio nacional de bacterias multirresistentes disponible para el estudio de la resistencia bacteriana en Chile / Creation of the first national biorepository of multi-resistant bacteria available for the study of bacterial resistance in Chile

La disponibilidad de cepas bacteriana para el estudio de la resistencia bacteriana es clave para los avances en la investigación básica y clínica respecto del tema. Existen pocos biorrepositorios o bancos de bacterias con mecanismos de resistencia conocidos, aisladas de infecciones clínicamente significativas. Una revisión de la literatura revela que sólo en los Estados Unidos de América existe un biobanco de aislados resistentes disponibles para estudios. En esta publicación se cuenta cómo se creó el primer biorrepositorio de bacterias resistentes en Chile asociados a la Red de Laboratorios MICROB-R, con la participación de 11 centros distribuidos a lo largo del país, que a la fecha cuenta con más de 3.500 aislados bacterianos estudiados fenotípica y genotípicamente, disponibles para la comunidad científica chilena.

The availability of bacterial strains for the study of bacterial resistance is key to advances in basic and clinical research. There are few biobanks of bacteria with known resistance mechanisms, isolated from clinically significant infections. A review of the literature reveals that only in the United States of America is there a biobank of resistant isolates. This publication shows the creation of the first biorepository of resistant bacteria Chile associated with the MICROB-R Laboratory Network, with the participation of 11 centers distributed throughout the country, which to date has more than 3,500 bacterial isolates studied phenotypically and genotypically, available to the Chilean scientific community.