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1.
CienciaUAT ; 15(2): 135-143, ene.-jun. 2021. graf
Article in Spanish | LILACS-Express | LILACS | ID: biblio-1285897

ABSTRACT

Resumen La oxidación de lípidos deteriora los alimentos, por lo que se usan antioxidantes sintéticos para disminuirla, sin embargo, estos compuestos en exceso poseen efectos carcinogénicos. Algunas plantas como el orégano, así como la miel de abeja, contienen antioxidantes naturales que no dañan la salud. Hasta el momento no se han encontrado registros del uso de la miel de abeja para disminuir la oxidación lipídica en carne de conejos. El objetivo de este estudio fue evaluar el efecto de la miel de abeja como antioxidante en la carne cruda de lomo de conejo almacenada en refrigeración a 4 °C. Se evaluó la actividad antioxidante (AA) de tres tipos de miel: oscura, ámbar y clara, para mezclarla con la carne de conejo. Se seleccionó la miel oscura por su mayor efecto antioxidante. Se prepararon 64 muestras de 100 g de carne cruda, 32 se mezclaron con 2 g de miel oscura y las otras 32 se dejaron sin miel (control). Las muestras se almacenaron a 4°C y se evaluó la AA y la concentración de malondialdehído (MDA) a los 0 d, 3 d, 6 d y 9 d de almacenamiento. La AA disminuyó y la concentración de MDA aumentó (P < 0.05) con el tiempo de refrigeración (cambios que indican deterioro de la carne). A los 3 d y 6 d, las muestras de carne cruda con miel exhibieron mayor AA (P < 0.05), y a los 6 d, menores valores de MDA (P < 0.05) comparadas con las muestras control (indicando que no hubo deterioro de la carne). La miel oscura de abeja contiene altas concentraciones de antioxidantes naturales que protegen a la carne cruda molida de lomo de conejo contra el daño oxidativo que puede presentarse durante la refrigeración, por lo que se recomienda su uso para este fin.


Abstract Lipid oxidation deteriorates foods; therefore, synthetic antioxidants are used to decrease it. However, excess synthetic antioxidants have carcinogenic effects. Some plants such as oregano, as well as bee honey, contain natural antioxidants which are not harmful to health. No reports were found on the use of bee honey to decrease lipid oxidation in rabbit meat. The objective of this study was to assess the effect of bee honey as antioxidant on raw rabbit loin, refrigerated at 4 °C. The antioxidant activity (AA)of three types of honey was evaluated: dark, amber and clear, to be mixed with rabbit meat. Dark honey was selected for its higher antioxidant effect. 64 samples of 100 g of raw meat were prepared, 32 samples were mixed with 2 g dark honey and the other 32 were left without honey (control). The samples were stored at 4 °C and AA as well as malondialdehyde (MDA) concentrations were evaluated at 0 d, 3 d, 6 d and 9 d of storage. The AA decreased, and the MDA concentration increased (P < 0.05) with refrigeration time (changes that indicate meat spoilage). After 3 d and 6 d, the raw meat samples with honey showed higher AA (P < 0.05) and after 6 d, they showed lower MDA values (P < 0.05) compared to the control samples (these changes indicate no spoilage of meat). Dark bee honey contains high concentrations of natural antioxidants that protect ground raw rabbit loin against oxidative damage that can occur during refrigeration, therefore, its use is recommended for this purpose.

2.
Rev. colomb. cienc. pecu ; 33(2): 96-109, Apr.-June 2020. tab
Article in English | LILACS-Express | LILACS | ID: biblio-1289287

ABSTRACT

Abstract Background: Carcass and sheep meat quality are influenced by several factors, such as race, age, slaughter weight, type of diet, and dietary supplementation. Objective: To evaluate the effect of castration and vitamin E supplementation on performance, carcass characteristics and meat of lambs slaughtered at 45 kg. Methods: Thirty-two male lambs (16 castrated and 16 not castrated) were fed diets with or without the inclusion of vitamin E. The animals had an average age of 180 ± 20 days and a mean weight of 25.1 ± 4.6 kg. Performance was evaluated for 56 days, and the animals were slaughtered when reaching a weight of 45 kg. The experimental design was randomized blocks based on initial weights, and variables were assessed by a 2x2 factorial design (castrated and intact, supplemented or not with vitamin E, with a probability of 5%). Results: Higher proportion (p<0.05) of visceral fat was found in castrated animals (kidney: 2.45%, inguinal: 0.57% and omental: 4.96%) than in intact animals (kidney: 1.25%, inguinal 0.27%. and omental 3.12%). The aged meat samples showed no color differences for any of the treatments, but there were differences in shear force for steaks aged for 7 days, where the castrated animals showed an average of 2.64 kgf and intact animals showed an average 3.29 kgf. The animals supplemented with vitamin E had lower shear forces in non-aged samples. Conclusion: Castration increased deposition of visceral and fat coverage but did not influence the cut yields and total carcass yield. The animals supplemented with vitamin E had lower shear forces in non-aged samples.


Resumen Antecedentes: La calidad de la canal y de la carne de ovino puede ser influenciada por varios factores, como raza, edad, peso de sacrificio, tipo de dieta y suplementación. Objetivo: Evaluar el efecto de la castración y de la suplementación con vitamina E sobre el desempeño, la calidad de la canal y la carne de corderos Santa Inés sacrificados a los 45 kg de peso. Métodos: Fueron utilizados treinta y dos corderos: dieciséis castrados y dieciséis no castrados, alimentados con dietas con o sin inclusión de vitamina E. Los animales tenían una edad promedio de 180 ± 20 días y un peso promedio de 25,1 ± 4,6 kg. Fue evaluado el desempeño durante 56 días y, al llegar a los 45 kg de peso fueron sacrificados. El diseño experimental fue en bloques aleatorizados con base en el peso inicial, y las variables analizadas mediante un modelo factorial 2x2 (castrado o no castrado y suplementado o no con vitamina E, con una probabilidad del 5%). Resultado: Se encontró mayor proporción (p<0,05) de grasa visceral en los animales castrados (renal: 2,45%; inguinal: 0,57% y mesentérica: 4,96%) que en los no castrados (renal: 1,25%; inguinal 0,27% y mesentérica: 3,12%). Las muestras de carne maduradas no presentaron diferencias de color entre tratamientos, sin embargo hubo diferencias en la fuerza de corte para las carnes maduradas por 7 días, donde los animales castrados presentaron una media de 2,64 kgf y los no castrados de 3,29 kgf. La carne de los animales suplementados con vitamina E tuvo menor fuerza de corte en las muestras no maduradas. Conclusión: La castración aumentó la deposición de grasa visceral y de cobertura pero no influenció las características de rendimiento total de la canal y cortes. Los animales suplementados con vitamina E tuvieron menor fuerza de corte en las muestras no maduradas.


Resumo Antecedentes: A qualidade da carcaça e da carne ovina pode ser influenciada por vários fatores como raça, idade, peso de abate tipo de dieta e suplementação. Objetivo: Avaliar o efeito da castração e da suplementação de vitamina E sobre o desempenho e qualidade de carcaça e carne de cordeiros Santa Inês abatidos a 45 kg de peso. Métodos: Foram utilizados trinta e dois cordeiros, dezesseis castrados e dezesseis não castrados alimentados com dietas com ou sem inclusão de vitamina E. Os animais tinham idade média de 180 ± 20 dias e peso médio de 25,1 ± 4,6 kg. Foi avaliado o desempenho durante 56 dias, e ao chegarem aos 45 kg foi realizado o abate. O delineamento experimental foi em blocos ao acaso, com base no peso inicial, e as variáveis analisadas por modelo fatorial 2x2 (castrado ou não castrado e suplementado ou não com vitamina E, ao nível de 5%). Resultado: Foi encontrada maior proporção (p<0,05) das gorduras viscerais nos animais castrados (renal: 2,45%; inguinal: 0,57% e omental: 4,96%) do que nos animais não castrados (renal: 1,25%; inguinal 0,27% e omental: 3,12%). As amostras maturadas não apresentaram diferenças de cor entre os tratamentos, no entanto houve diferenças na força de cisalhamento para as carnes maturadas por 7 dias, na qual os animais castrados apresentaram média de 2,64 kgf e os não castrados de 3,29 kgf. Os animais suplementados com vitamina E apresentaram menores forças de cisalhamento nas amostras não maturadas. Conclusão: A castração aumentou a deposição de gorduras viscerais e de cobertura, mas não influenciou as características de rendimento de carcaça e cortes. Os animais suplementados com vitamina E tiveram menor força de cisalhamento nas amostras não maturadas.

3.
Arq. bras. med. vet. zootec. (Online) ; 72(1): 224-232, Jan.-Feb. 2020. tab
Article in English | LILACS, VETINDEX | ID: biblio-1088925

ABSTRACT

A total of 640 one-day-old Cobb broiler chicks, half male and half female, were reared up to 49 days of age. A completely randomized 2 × 4 factorial design was used. Experimental treatments consisted of four levels of organic zinc supplementation and one control (inorganic zinc supplementation) and two levels of sex (female and male), with four replicates of 16 birds per experimental plot. Animal performance, carcass and cut yields, and meat quality were evaluated. Feed intake increased with zinc supplementation. Broilers that received the lowest level of organic zinc showed better feed conversion than those fed an equivalent level of inorganic zinc. Increased levels of organic zinc reduced the abdominal fat content in females but impaired the oxidative stability of meat. The results showed that male broilers have better performance, carcass yield, and meat quality than females and that organic zinc improves broiler feed conversion and reduces abdominal fat but increases lipid oxidation in meat.(AU)


Foram alojados 640 pintainhos de corte, sendo metade de machos e metade de fêmeas, da linhagem Cobb, de um a 49 dias de idade. Foi adotado um delineamento inteiramente ao acaso, em esquema fatorial 2 × 4. Os tratamentos experimentais consistiram em quatro níveis de suplementação de zinco orgânico e um controle inorgânico, e diferentes sexos, com quatro repetições de 16 aves por parcela experimental. Foram avaliados o desempenho, a característica da carcaça e dos cortes, bem como a qualidade de carne dos frangos. O aumento dos níveis de zinco elevou o consumo de ração dos frangos. Os frangos que receberam zinco orgânico na exigência mínima apresentaram melhor conversão alimentar comparados àqueles que receberam ração de fonte inorgânica. O aumento nos níveis de zinco orgânico proporcionou redução no teor de gordura abdominal para fêmeas, mas níveis de zinco orgânico prejudicaram a estabilidade oxidativa da carne. Conclui-se que frangos machos apresentam melhor desempenho, carcaça e qualidade de carne em relação às fêmeas, e que o zinco orgânico melhora a conversão alimentar dos frangos, reduz a gordura abdominal, porém eleva a oxidação lipídica da carne.(AU)


Subject(s)
Animals , Trace Elements/administration & dosage , Zinc/administration & dosage , Weight Gain , Chickens/growth & development , Meat/analysis
4.
Ciênc. rural (Online) ; 50(1): e20190344, 2020. tab, graf
Article in English | LILACS-Express | LILACS | ID: biblio-1055850

ABSTRACT

ABSTRACT: The aim of this study was to evaluate the effect of the inclusion of microbial biomass on the oxidative rancidity of tilapia rations stored for 12 months. Treatments included a control diet and diets supplemented with either 0.01% vitamin E, 0.25 and 0.5% of Saccharomyces cerevisiae and 0.25 and 0.5% of Spirulina platensis. Experimental diets were stored in the dark inside plastic bags at room temperature (25 °C) for 12 months. The oxidative rancidity was measured as thiobarbituric acid reactive substances (TBARS). It was concluded that the inclusions of Spirulina platensis at 0.25% (1.734 ± 0.206) and 0.5% (1.629 ± 0.181) and Saccharomyces cerevisiae at 0.5% (1.459 ± 0.305) minimized the oxidative rancidity in comparation to control diet (2.843 ± 0.109) of Nile tilapia until 12 months of storage.


RESUMO: O objetivo deste estudo foi avaliar o efeito da inclusão de biomassa microbiana sobre a rancidez oxidativa de rações de tilápia armazenadas por 12 meses. Os tratamentos incluíram uma dieta controle e dietas suplementadas com 0,01% de vitamina E 0,01%, com 0,25 e 0,5% de S. cerevisiae e com 0,25 e 0,5% de S. platensis. As dietas experimentais foram armazenadas em sacos plásticos escuros, em temperatura ambiente (25 °C), durante 12 meses. A rancidez oxidativa foi mensurada pelas substâncias reativas ao ácido tiobarbitúrico (TBARS). Concluiu-se que as inclusões de 0,25% (1,734 ± 0,206) e 0,5% (1.629 ± 0.181) de Spirulina platensis e 0,5% (1.459 ± 0.305) de Saccharomyces cerevisiae reduziram a rancidez das dietas em comparação a dieta controle de tilápias-do-Nilo após 12 meses de armazenamento.

5.
Ciênc. rural (Online) ; 50(11): e20200287, 2020. tab, graf
Article in English | LILACS-Express | LILACS | ID: biblio-1133224

ABSTRACT

ABSTRACT: The aim of the present study was to compare the color and oxidative stability of light (LM) and dark (DM) muscles of Nile tilapia (Oreochromis niloticus) stored at 4 °C for eight days. Five different trials of LM and DM samples were analyzed for instrumental color attributes (lightness, redness and yellowness), including the surface color stability through ratio of reflectance at 630/580 nm (R630/580), myoglobin concentration, total lipid content, fatty acid profile, metmyoglobin reducing activity (MRA), pH, lipid oxidation and protein oxidation. Results of the present study indicated that DM of Nile tilapia (Oreochromis niloticus) present lower oxidative and color stability during refrigerated storage than LM.


RESUMO: O objetivo do presente estudo foi comparar a estabilidade de cor e oxidativa de músculos claros (LM) e escuros (DM) de tilápia do Nilo (Oreochromis niloticus) armazenados a 4° C por oito dias. Cinco repetições diferentes de amostras de LM e DM foram analisadas quanto aos atributos instrumentais de cor (luminosidade, teor de vermelho e teor de amarelo), incluindo a estabilidade de cor na superfície através da razão de refletâncias nos comprimentos de onda 630 e 580 nm (R630/580), concentração de mioglobina, lipídios totais, perfil de ácidos graxos, atividade de redução da metamioglobina (MRA), pH, oxidação lipídica e oxidação proteica. Os resultados do presente estudo indicam que músculos escuros (DM) de tilápia do Nilo (Oreochromis niloticus) apresentam menor estabilidade oxidativa e de cor durante o armazenamento refrigerado, quando comparados aos músculos claros (LM).

6.
Rev. med. vet. (Bogota) ; (38): 101-109, ene.-jun. 2019. tab
Article in Spanish | LILACS-Express | LILACS | ID: biblio-1094066

ABSTRACT

Resumen El objetivo del presente estudio fue evaluar el efecto sobre la calidad espermática de tres substancias antioxidantes (50 µM de trolox/108 espermatozoides, 50 µg de catalasa/ml de eyaculado y cisteamina 5mM) en una base de diluyente comercial Triladyl™, bajo condiciones de refrigeración y congelación/descongelación en semen ovino. Se congeló semen de seis machos adultos en una base del diluyente comercial Triladyl™. El eyaculado de cada macho fue puesto en cuatro diferentes alícuotas: una para control, y a las demás se les adicionó con la base del diluyente 50 µM de trolox/108 espermatozoides, 50 µg de catalasa/ml de eyaculado y cisteamina 5 mM. La calidad espermática precongelación (5 °C) y poscongelación se evaluó con la ayuda de un sistema de análisis computarizado (IVOS II®), con lo cual se eliminó la subjetividad a la prueba. Se observó que 50 µM de trolox/108 SPZ y 50 µg de catalasa/ml tienen la capacidad de mantener valores (p < 0,05) superiores de motilidad total (64,26 ± 0,82 y 55,54 ± 0,85) y motilidad progresiva (47,26 ± 0,75 y 44,32 ± 1,13) en condiciones de precongelación, y para motilidad total (67,60 ± 1,91 y 63,47 ± 3,40), motilidad progresiva (50,87 ± 2,58 y 38,88 ± 2,31) y viabilidad (66,97 ± 2,25 y 61,16 ± 4,31) en poscongelación, comparados con los tratamientos control o la adición de 5 mM de cisteamina. La adición de 50 µM de trolox/108 espermatozoides y de 50 µg de catalasa/ml de semen mejora la motilidad total y progresiva del semen ovino refrigerado a 5 °C y la viabilidad en semen congelado/descongelado.


Abstract The present study aimed to evaluate the effect on sperm quality of three antioxidant substances (50 μM of trolox/108 spermatozoa, 50 μg of catalase/ml of ejaculate, and 5mM cysteamine) in a Triladyl™ commercial diluent base, under conditions of refrigeration and freezing/thawing in sheep semen. Semen from six adult males was frozen in a commercial Triladyl™ diluent base. The ejaculate of each male was placed in four different aliquots: one for control, and the other three with additional 50 μM of trolox/108 spermatozoa, 50 μg of catalase/ml of ejaculate, and 5 mM cysteamine, respectively, in the diluent base. Pre-freezing (5 °C) and post-freezing sperm quality was evaluated using a computerized analysis system (IVOS II®), which eliminated subjectivity during the test. It was observed that 50 μM of trolox/108 SPZ and 50 μg of catalase/ml are able to maintain higher values (p < 0.05) of total motility (64.26 ± 0.82 and 55.54 ± 0.85 ) and progressive motility (47.26 ± 0.75 and 44.32 ± 1.13) under pre-freezing conditions, and of total motility (67.60 ± 1.91 and 63.47 ± 3.40), progressive motility (50.87 ± 2.58 and 38.88 ± 2.31), and viability (66.97 ± 2.25 and 61.16 ± 4.31) in the post-freezing period, compared to control treatments or the addition of 5 mM of cysteamine. The addition of 50 μM of trolox/108 spermatozoa and 50 μg of catalase/ml of semen improves the total and progressive motility in ovine semen refrigerated at 5 °C, as well as viability in frozen/thawed semen.


Resumo O objetivo do presente estudo foi avaliar o efeito sobre a qualidade espermática de três substancias antioxidantes (50 µM de trolox/108 espermatozoides, 50 µg de catalasa/ml de ejaculado e cisteamina 5mM) numa base de diluente comercial Triladyl™, sob condições de refrigeração e congelamento/descongelamento em sêmen ovino. Sêmen de seis machos adultos foi congelado numa base do diluente comercial Triladyl™. O ejaculado de cada macho foi posto em quatro diferentes alíquotas: uma para controlo e nas outras foi adicionada a base do diluente 50 µM de trolox/108 espermatozoides, 50µg de catalasa/ml de ejaculado e cisteamina 5mM. A qualidade espermática precongelação (5 °C) e pós-congelação avaliou-se com ajuda de um sistema de análise computadorizado (IVOS II®), eliminando assim a subjetividade ao teste. Observou-se que 50 µM de trolox/108 SPZ e 50 µg de catalasa/ml têm a capacidade de manter valores (p < 0,05) superiores de motilidade total (64,26 ± 0,82 e 55,54 ± 0,85) e motilidade progressiva (47,26 ± 0,75 e 44,32 ± 1,13) em condições de pre-congelação, e para motilidade total (67,60 ± 1,91 y 63,47 ± 3,40), motilidade progressiva (50,87 ± 2,58 e 38,88 ± 2,31) e viabilidade (66,97 ± 2,25 e 61,16 ± 4,31) em pós-congelação, comparados com os tratamentos controle ou a adição< de 5 mM de cisteamina. A adição de 50 µM de trolox/108 espermatozoides e 50 µg de catalasa/ml de sêmen melhora a motilidade total e progressiva do sêmen ovino refrigerado a 5 °C e a viabilidade em sêmen congelado/descongelado.

7.
Ciênc. rural (Online) ; 49(1): e20180797, 2019. tab, graf
Article in English | LILACS | ID: biblio-1045234

ABSTRACT

ABSTRACT: Edible chitosan-based films, in pure form (CH100), or blended with gelatin (GEL50:CH50), with and without boldo extract (B) were prepared. The oxygen permeability of the films, as well as color changes and antioxidant and antimicrobial activities on beef hamburgers samples were evaluated. CH100+B film exhibited optimal oxygen barrier properties (6.40±0.170cc-mL/[m²-day]), and this value was significantly increased in blended films (GEL50:CH50+B = 12.99±1.50cc-mL/[m²-day]) (P<0.05). All films provoked color changes in hamburger samples during storage, and the samples covered with CH100+B film showed a lower brightness and a higher redness. All films effectively protected beef hamburger from lipid oxidation, and boldo extract incorporation in pure and blended films enabled significant protection, when compared to the control sample (155.78±0.85meq O2/kg) (P<0.05). Finally, all films proved to be effective in microbial growth control, suggesting that these films are an effective alternative in improving food safety and quality of fresh meat.


RESUMO: Filmes comestíveis à base de quitosana, na forma pura (CH100), ou misturados com gelatina (GEL50:CH50), com e sem extrato de boldo (B), foram preparados. Avaliou-se a permeabilidade ao oxigênio dos filmes, assim como mudanças de cor e atividades antioxidantes e antimicrobianas sob amostras de hambúrguer de carne bovina. Filmes de CH100+B exibiram ótimas propriedades de barreira ao oxigênio (6,40±0,170cc-mL/[m²-dia]), e este valor foi significativamente aumentado em filmes mistos (GEL50:CH50+B = 12,99±1,50cc-mL/[m²-dia]) (P<0,05). Todos os filmes provocaram mudanças de cor nas amostras de hambúrgueres durante o armazenamento, apresentando as amostras cobertas com o filme CH100+B uma menor luminosidade e um maior vermelhidão. Todos os filmes protegeram efetivamente o hambúrguer de carne bovina da oxidação lipídica, e a incorporação do extrato de boldo em filmes puros e misturados possibilitou significativa proteção, quando comparada à amostra controle (155,78±0,85 meq O2/kg) (P<0,05). Finalmente, todos os filmes mostraram-se eficazes no controle do crescimento microbiano, sugerindo que estes filmes são uma alternativa eficaz para melhorar a segurança dos alimentos e a qualidade da carne fresca.

8.
Ciênc. rural (Online) ; 49(8): e20190090, 2019. tab, graf
Article in English | LILACS | ID: biblio-1045423

ABSTRACT

ABSTRACT: In order to improve nutritional and functional quality, chicken burgers were made with different amounts of chia seed (0, 2, 4 and 8%) as pork fat replacers and evaluated for the microbiological, technological and sensorial characteristics. The proximate composition and the microbiological characteristics of raw burgers were in accordance with Brazilian legislation. Higher additions of chia increased the amount of dietary fiber (up to 1.46%) and carbohydrate in the products and reduced the fat content by up to 29%. The addition of chia seed reduced the a* values and increased the h* values of the raw burgers and reduced the tenderness and degree of lipid oxidation in the cooked burgers. Lower sensory taste scores and overall acceptance were conferred on products with higher chia addition; although, the degree of acceptance of the products was high. Results of this study highlighted the potential use of chia seed in the elaboration of low fat and enriched dietary fiber burgers.


RESUMO: No intuito de melhorar a qualidade nutricional e funcional, hambúrgueres de frango foram elaborados com diferentes quantidades de semente de chia (0, 2, 4 e 8%) em substituição ao toucinho e avaliados quanto às características microbiológicas, tecnológicas e sensoriais. A composição centesimal e as características microbiológicas dos hambúrgueres crus estavam de acordo com o preconizado pela legislação Brasileira. Maiores adições de chia aumentaram a quantidade de fibra dietética (até 1,46%) e de carboidrato nos produtos e reduziu os valores de gordura em até 29%. A adição de semente de chia reduziu os valores de a* e aumentou os valores de h* da cor dos hambúrgueres crus e reduziu a maciez e o grau de oxidação lipídica nos hambúrgueres cozidos. Menores notas sensoriais de sabor e a aceitação global foram conferidas aos produtos com maior adição de chia, embora o grau de aceitação dos produtos tenha sido elevado. Os resultados deste estudo destacam o potencial de uso da semente de chia na elaboração de hambúrgueres com baixo teor de gordura e enriquecidos de fibra dietética.

9.
Braz. arch. biol. technol ; 61: e18180055, 2018. tab
Article in English | LILACS | ID: biblio-974059

ABSTRACT

ABSTRACT The oxidative stability of linseed (L), cotton (A), and coconut (C) oils, as well as of linseed:cotton (LA), linseed:coconut (LC), and linseed:cotton:coconut (LAC) compound oils was evaluated under accelerated storage at 60°C/20 days. Coconut oil showed to be rather stable, mainly due to low levels of peroxides, conjugated dienes, ρ-anisidine, and long induction period. In addition, along with cotton oil, it improved the stability of linseed oil in the formulation of LAC compound oil. As to fatty acid profile, the compound oils showed to be composed mainly by unsaturated fatty acids. Cotton and coconut oils presented higher retention of total phytosterols, 78.87 and 76.16%, respectively, after 20 days of storage, when compared to linseed oil. The highest retention of total tocopherols at the end of storage was observed in LA (90.81%). In relation to antioxidant activity, by the DPPH method, with the increase in storage time, a reduction in the antioxidant substances of linseed, LC, and LAC oils was observed. Through the FRAP method, oscillations were observed, especially in linseed and compound oils. Although the oils were degraded over time, it was possible to verify that cotton and coconut oils contributed to increase the stability of linseed oil, which, in turn, raised the levels of coconut oil bioactive compounds.

10.
Pesqui. vet. bras ; 37(12): 1405-1410, dez. 2017. tab, graf
Article in English | LILACS, VETINDEX | ID: biblio-895406

ABSTRACT

This study was conducted to assess the changes in some indicators of oxidative status during pregnancy and lactation in sheep of different parity. Dorset x Suffolk ewes were classified by number of parity: 1, 2 and ≥4. They were sampled before pregnancy and on the first, second, third, and fourth months, then on day 143 of pregnancy, as well as on day 5 after birth and after one month of lactation. Antioxidant capacity was found to have two reductions, the first during the second month of pregnancy and the second on day 5 of lactation. Susceptibility to lipid oxidation decreased with an increased number of parturitions. A reduction in lipid oxidation was observed on day 143 of gestation relative to the other samplings during gestation and lactation. Total glutathione peroxidase activity increased when the two reductions in antioxidant capacity took place. Ascorbic acid decreased during lactation and gestation; the lowest values were recorded in the third month of gestation. It is concluded that susceptibility to lipid oxidation decreases with the number of parturitions and that in ewes, during gestation and lactation, there is a mechanism that prevents lipid oxidation involving changes in antioxidant capacity, glutathione peroxidase and ascorbic acid.(AU)


Este estudo foi conduzido para avaliar as mudanças em alguns indicadores do estado oxidativo durante a gestação e lactação em ovelhas com diferentes números de partos. Ovelhas Dorset x Sufolk foram classificadas pelo número de partos: 1, 2 e ≥4. Amostras foram coletadas antes da prenhez e no primeiro, segundo, terceiro e quarto mês e no dia 143 de gestação, assim como no dia 5 após o parto e com um mês de lactação. Encontrou-se que a capacidade antioxidativa teve duas reduções, a primeira durante o segundo mês de gestação e a segunda no dia 5 de lactação. A atividade total da glutationa peroxidase aumentou quando se deram as reduções de capacidade antioxidante. O ácido ascórbico diminuiu durante a gestação e lactação, com o valor mais baixo foi no terceiro mês de gestação. Conclui-se que a suscetibilidade a oxidação diminui com o número de partos, e que nas ovelhas durante a gestação e lactação há um mecanismo que previne a oxidação lipídica ocasionando mudanças na capacidade antioxidante, e das atividades glutationa peroxidase e ácido ascórbico.(AU)


Subject(s)
Animals , Female , Pregnancy , Ascorbic Acid , Oxidative Stress , Peripartum Period , Glutathione Peroxidase , Lipids/chemistry
11.
Rev. colomb. quím. (Bogotá) ; 46(2): 17-23, may.-ago. 2017. graf
Article in Spanish | LILACS | ID: biblio-900820

ABSTRACT

Resumen Se obtuvieron fracciones a partir del epicarpio de tomate de árbol (Solanum betaceum Sendtn) y se evaluó su efecto protector contra la oxidación lipídica en carne de res cocida (CRC). Un extracto obtenido a través de extracción supercrítica fue fraccionado empleando, a su vez, extracción en fase sólida; dichas fracciones fueron adicionadas a CRC (concentración 200 mg/kg). El efecto protector fue determinado a través de la comparación de algunos productos de oxidación y el antioxidante sintético terbutilhidroxiquinona (TBHQ, concentración 200 mg/kg). Cuatro fracciones fueron obtenidas, la fracción de mayor polaridad mostró una alta eficiencia para inhibir la oxidación lipídica en CRC, reduciendo la formación de hidroperóxidosHPL y TBARS en 100 y 98,5%, respectivamente; esta mostró una eficiencia superior a la observada para el TBHQ. Esta fracción más activa fue sometida a análisis HPLC-DAD y se identificaron algunos compuestos fenólicos: tres catequinas (epigalocatequina, epicatequina y galato de epigalocatequina) y un ácido fenólico (rosmarínico). Los resultados obtenidos permitieron evidenciar que el epicarpio de tomate de árbol es fuente de antioxidantes con efecto protector sobre CRC, alternativa de aprovechamiento y valorización para dicho residuo agroindustrial.


Abstract Fractions from tree tomato (Solanum betaceum Sendtn) epicarp were obtained and their effect against lipid oxidation in cooked beef meat (CBM) was evaluated. An extract obtained by supercritical fluids extraction was fractionated using solid phase extraction and these fractions were added to CBM (at 300 mg/kg). The protector effect against lipid oxidation was determined by measuring some lipid oxidation products and comparison against the synthetic antioxidant tert-butylhydroquinone (TBHQ at 300 mg/ kg). Four fractions were obtained, the most polar fraction showed high efficiency to reduce the lipid oxidation on CBM, this fraction reduced the LHP and TBARS formation at 100.0 and 98.5%, respectively. This fraction showed an ability to retard lipid oxidation in CRC higher than TBHQ. The fraction with the highest protector effect was analyzed for its phenolic composition by High-Performance Liquid Chromatography with Diode-Array Detection (HPLC-DAD). Three catechin (epigallocatechin, epicatechin and epigallocatechin gallate) and a phenolic acid (rosmarinic acid) were identified. The results revealed that the tree tomato epicarp is a source of antioxidants with protective effect on CBM, an alternative for the use and valorization of this agroindustrial waste.


Resumo Frações da casca de tomate de árvore (Solanum betaceum Sendtn) foram obtidas e seu efeito para retardar a oxidação dos lipídios em carne bovina cozida (CBC) foi avaliado. Um extrato obtido através da extração supercrítica foi fracionado utilizando extração em fase sólida e as frações obtidas foram adicionadas á CBC (concentração 300 mg/kg). O efeito protetor contra a oxidação dos lipídios foi determinado medindo alguns produtos de oxidação e comparando com a terbutil-hidroquinona (TBHQ, concentração 300 mg/kg). Quatro frações foram obtidas, destas, a fração de maior polaridade mostrou a maior eficiência para inibir a oxidação dos lipídios da CBC, reduzindo a formação de HPL e TBARS em 100 e 98,5%, respectivamente; mostrou uma eficiência maior da observada para TBHQ. Esta foi submetida a análise por a cromatografia líquida de alta eficiência com detector de díodos (HPLC-DAD) e foram identificados alguns compostos fenólicos: três catequinas (epigalocatequina, galato de epicatequina e galato de epigalocatequina) e um ácido fenólico (rosmarínico). Os resultados permitiram evidênciar que a casca do tomate de árvore é uma fonte de compostos antioxidantes comefeito protetor sobre a CBC, esta é uma alternativa de utilização e aproveitamento deesse resíduo agroindustrial.

12.
Chinese Journal of Rehabilitation Medicine ; (12): 1139-1142, 2017.
Article in Chinese | WPRIM | ID: wpr-666358

ABSTRACT

Objective:To observe the lipid oxidation rates in spinal cord injury (SCI) patients with different exercise intensities.Method:Thirty sedentary patients with SCI were selected to perform incremental exercise load tests (baseline test and Fatmax test) with upper limb power vehicle.Meanwhile,both tests included respiratory gas analysis.In baseline tests,the workload was started at 5W and increased by 10W every 3 min,with 60 rmp until patients exhausted.The Fatmax test included 5 stages of workload and every stage kept 6 min.The initial workload was 5W,the fifth workload was corresponded to the load when respiratory quotient was 1 in the baseline test,the other workload was set according to the average of the initial and fifth workload.All lipid oxidation rates were measured by oxygen uptake (VO2) and respiratory quotient (RQ) in the tests.Result:With the increase of exercise intensity,lipid oxidation rates in SCI patients increased at first and then decreased.The peak of lipid oxidation rates (5.46±0.75 μmol/kg/min) arrived at the intensities of 34.9%±2.3% VO2 peak.After that the lipid oxidation rates decreased significantly as 3.92±0.69,2.85±0.61,0.58±0.19 μmol/kg/min at 41.83±2.18,52.29±1.73 and 61.32±1.98% VO.2 peak accordingly(P<0.05).Conclusion:During the rehabilitation of SCI patients,we should pay attention to the intervention from low intensity exercise to maximize lipid oxidation rates,reducing the incidence of obesity in SCI patients.

13.
Chinese Journal of Schistosomiasis Control ; (6): 48-52,58, 2017.
Article in Chinese | WPRIM | ID: wpr-605992

ABSTRACT

Objective To study the effect of exogenous nitric oxide donor sodium nitroprusside(SNP)on antioxidant en?zymes activities and lipid peroxidation of mice infected with Trichinella spiralis. Methods BALB/c mice were infected with T. spiralis separated by the digestion method. Forty?two days post?infection,the peripheral blood and hepatic tissue from the in?fected or normal mice were collected. Then 4 groups were set:liver homogenate from infected mice+SNP(Group A),liver ho?mogenate from normal mice+SNP(Group B),peripheral blood from infected mice+SNP(Group C),and peripheral blood from normal mice+SNP(Group D). The final concentrations of SNP in each group were set as 0(blank control),2,5,10μmol/L and 30μmol/L,respectively. After reacting with SNP at 37℃for 30 min,the superoxide dismutase(SOD),glutathi?one peroxidase(GSH?Px),catalase(CAT)activities,and malondialdehyde(MDA)concentration were examined and com?pared. Results The levels of SOD,CAT,GSH?Px and MDA concentration in the liver and the blood from the mice infected with T. spiralis were significantly higher than those of the normal ones(all P < 0.05). When reacted with 10 μmol/L and 30 μmol/L SNP,the SOD,GSH?Px,and CAT activities in Group A and B decreased significantly(all P<0.05),while the liver MDA concentration reacted with 2-30μmol/L SNP increased obviously(all P<0.05). As reacted with 30μmol/L SNP,the ac?tivities of blood SOD,GSH?Px,and CAT in Group C and D decreased,while the MDA concentration in blood still increased (all P<0.01). When the SNP concentration was in the range of 2-30μmol/L,there were a negative correlation between the SNP concentrations and SOD,GSH?Px,and CAT activities,as well as a positive correlation with the MDA concentration in the liver and blood from the mice infected with T. spiralis(all P<0.05). Conclusions T. spiralis infection could cause oxidative damage to mice,and increase SOD,GSH?Px,and CAT activities. Nitric oxide released from SNP can decrease antioxidase activ?ities,and inhibit the antioxidant capacity of mice infected with T. spiralis.

14.
Ciênc. rural ; 46(3): 560-566, mar. 2016. graf
Article in English | LILACS | ID: lil-769683

ABSTRACT

ABSTRACT: This research aimed to evaluate whether the essential oil of Aloysia triphylla (EOAT) used in vivo as a sedative in the water for transporting fish could increase the oxidative stability of silver catfish (Rhamdia quelen) fillets during frozen storage. The chemical composition of EOAT and of fillets from fish exposed to EOAT (0, 30 or 40µL L-1) were assessed. The pH and lipid oxidation parameters (conjugated dienes, CD; thiobarbituric acid-reactive-substances, TBARS) were evaluated in the fillets throughout the storage period (-18±2oC/17 months). The main compounds found in EOAT were α- and β-citral. Treatment with EOAT did not modify the proximate composition of the fillets, but 40µL L-1 EOAT reduced pH levels when compared to the control fillets (P<0.05). Compared to the control fillets, the fillets from fish treated with 30 and 40µL L-1 EOAT had higher initial CD values (P<0.05), whereas fillets from fish treated with 40µL L-1 EOAT had lower TBARS levels after 6, 9 and 17 months of storage (P<0.05). Results indicated that use of EOAT as a sedative in silver catfish transport water delays the degradation of primary oxidation products (CD) into secondary products (TBARS) in the frozen fillets. This delay in the lipid oxidation rate may increase the shelf life of frozen fillets.


RESUMO: O objetivo do trabalho foi avaliar se o uso do óleo essencial de Aloysia triphylla (OEAT) na água de transporte de peixes, in vivo como sedativo, poderia aumentar a estabilidade oxidativa de filés de jundiá (Rhamdia quelen) durante o armazenamento congelado. Avaliou-se a composição química do OEAT e dos filés dos peixes expostos ao OEAT (0, 30 ou 40µL L-1), bem como o pH e indicadores de oxidação lipídica (dienos conjugados, DC; substâncias reativas ao ácido tiobarbitúrico, TBARS) dos filés ao longo do armazenamento (-18±2oC/17 meses). O α- e o β-citral foram os compostos majoritários do OEAT. O tratamento com OEAT não modificou a composição centesimal dos filés de jundiá, mas 40µL L-1 de OEAT reduziu o pH dos filés, comparado ao controle (P<0,05). Foi observado maior teor inicial de DC nos filés dos tratamentos 30 e 40µL L-1 de OEAT e menor valor de TBARS nos filés do tratamento 40µL L-1 de OEAT após 6, 9 e 17 meses de congelamento, em comparação com os filés controle (P<0,05). Os resultados indicam que o uso do OEAT como sedativo na água de transporte de jundiás retarda a degradação de produtos primários da oxidação lipídica (DC) em produtos secundários (TBARS) nos filés congelados. Este retardo na velocidade de oxidação lipídica pode ampliar a vida útil dos filés congelados.

15.
Journal of Chinese Physician ; (12): 223-225, 2016.
Article in Chinese | WPRIM | ID: wpr-493673

ABSTRACT

C1q /TNF-related proteins (CTRPs)belong to the same category of C1q /TNF-related protein family,mainly secreted by adipose tissue and widely expressed in human and rodent.Their function turned out to be metabolic modulation,cardiovascular system protection and suppression of inflammation.But for metabolic modulation,enhancing the synthesis of glycogen,promoting fatty acid uptake and oxidation,in-creasing insulin sensitivity is the major role of CTRPs playing in governing the metabolism.Each CTRP has its own unique tissue expression profile and nonredundant function in regulating sugar and /or fat metabo-lism.In this review we focus on the recent progress about the metabolic function of CTRPs.

16.
Article in English | IMSEAR | ID: sea-168502

ABSTRACT

This study was designed to investigate the influence of vacuum packaging on nutritional, chemical and microbial parameters of rohu fillets during frozen storage. Quality assessment of vacuum packaging rohu for up to 1 month at -12°C was done by the monitoring of nutritional quality, free fatty acids (FFA), thiobarbituric acid (TBA), pH and expressible moisture (EM). Results showed that free fatty acid, primary and secondary oxidation products, expressible moisture and pH value of vacuum packaging samples were significantly lower than those in control samples (p<0.05). Results indicated that vacuum packaging was effective in reduce lipid oxidation and increased shelf life of rohu frozen fillets. Similarly the microbial load of vacuum packaging samples was significantly lower as compared to control samples. Thus the employment of vacuum packaging alone or in combination with other protective strategies is recommended.

17.
Ciênc. rural ; 44(9): 1705-1710, 09/2014. tab, graf
Article in English | LILACS | ID: lil-725389

ABSTRACT

This study investigated the effects of hydroethanolic E. japonica seed extracts (EJSE) as inhibitors of lipid and protein oxidation on fish pates subjected to refrigerated storage. Five fish pate formulations were developed. These formulations included two control pates (water-control and ascorbic acid-control) and three pates with added EJSE (0.1, 0.2 and 0.4g of seed 100g-1 product, equivalent to 3.4, 6.8 or 13.6mg phenolic compounds kg-1 product), which were then stored under refrigeration for 35 days. Conjugated dienes (CD) and peroxide (PV) values increased along with the storage time; however, these values decreased and were similar among all samples at the end of 35 days of analysis (P<0.05). However, the thiobarbituric acid reactive substances levels (TBARS) did not change along the storage and were not affected by the EJSE. Additionally, there was a linear increase in the protein carbonyl content of fish pates over the storage period (P<0.05), but no effect of EJSE on protein oxidation. The results show that, at the concentrations evaluated, hydroethanolic E. japonica seed extract was unable to inhibit or reduce lipid and protein oxidation in fish pates, but the observed phenolic content emphasizes the need for further studies on the wastes of this fruit.


Este trabalho investigou os efeitos do extrato hidroetanólico de semente de E. japonica (EJSE) como inibidor da oxidação lipídica e proteica em patês a base de pescado armazenados refrigerados. Foram desenvolvidas cinco formulações de patê de pescado. Estas formulações incluíram dois patês controles (controle-água e controle-ácido ascórbico) e três adicionados de EJSE (0,1; 0,2 e 0,4g de semente 100g-1 de produto, equivalente a 3,4; 6,8 ou 13,6mg compostos fenólicos kg-1 de produto) que foram armazenados refrigerados durante 35 dias. Os valores de dienos conjugados (CD) e peróxidos (PV) aumentaram ao longo do armazenamento, contudo, CD e PV diminuíram de maneira semelhante em todas as amostras aos 35 dias de análise (P<0,05). No entanto, o conteúdo de substâncias reativas ao ácido tiobarbitúrico (TBARS) não se modificou ao longo do armazenamento e não foi afetado pelo EJSE. Também houve aumento linear no conteúdo de proteínas carboniladas dos patês de pescado ao longo do armazenamento (P<0,05), sem efeito do EJSE na oxidação proteica. Os resultados mostram que, nas concentrações avaliadas, o extrato hidroetanólico de semente de E. japonica não foi capaz de inibir ou reduzir as oxidações lipídicas e proteicas em patês de pescado, mas seu conteúdo fenólico enfatiza para a necessidade de aprofundar as pesquisas com o resíduo desta fruta.

18.
Arq. bras. med. vet. zootec ; 66(1): 185-194, fev. 2014. ilus, tab
Article in English | LILACS | ID: lil-704023

ABSTRACT

The objective was to evaluate the use of ractopamine (RAC) in the diet for pacu (Piaractus mesopotaminus) in the finishing phase on some quality parameters of the fillets. Thirty-five animals weighing 0.868±0.168kg were distributed in a completely randomised design with five treatments (0.0 - control; 11.25, 22.50, 33.75 and 45 ppm of RAC) and seven replicates with two fillets obtained from the same animal. The diets were isocaloric and isoprotein and experimental time was 90 days. RAC did not affect (P>0.05) the initial pH or ph after 24 hours of the fillets. Compared to the control, RAC increased (P<0.05) the moisture content of the fillets in natura and lipid oxidation of samples stored for 12 days in the refrigerator or freezer for 60 days. The RAC in 11.25 ppm reduced (P<0.05) the lipid content, while 45 ppm reduced (P<0.05) the crude protein in the fillets. Considering only RAC, there was a linear increase (P<0.05) in the lipid content (P<0.05) and a linear reduction in crude protein and weight loss after cooking the fillets. There was a quadratic effect (P<0.05) on the ash content, weight loss and lipid oxidation in fillets stored in the refrigerator or freezer. A RAC dose of 33.75 ppm resulted in a lower lipid oxidation index. In conclusion, ractopamine at 11.25 ppm is effective for reducing the fat content in fillets of pacu, although it increases the formation of peroxides in samples kept in the freezer for longer than 60 days. At 33.75 ppm, ractopamine is effective in reducing the effect of oxidation during storage in the refrigerator or freezer.


O objetivo foi avaliar a influência do uso de ractopamina na alimentação de pacus (Piaractus mesopotaminus) na fase de terminação sobre alguns parâmetros de qualidade dos filés. Trinta e cinco animais com peso de 0,868±0,168kg foram distribuídos em delineamento inteiramente ao acaso com cinco tratamentos (0,0 - controle; 11,25; 22,50; 33,75 e 45ppm de ractopamina) e sete repetições, sendo a parcela representada por dois filés provenientes de um animal. As rações foram isoenergéticas e isoproteicas, e o tempo experimental foi de 90 dias. A ractopamina não influenciou (P>0,05) o pH inicial dos filés e nem após 24 horas. Comparado ao controle, a RAC aumentou (P<0,05) a umidade dos filés in natura e a oxidação lipídica dos mesmos quando armazenados por 12 dias em geladeira ou 60 dias em freezer. Na dose de 11,25 reduziu (P<0,05) o teor de extrato etéreo e, na dose de 45 ppm, reduziu (P<0,05) o de proteína bruta dos filés. Considerando somente a RAC, houve aumento linear (P<0,05) do teor de gordura e redução linear (P<0,05) da proteína bruta e perda de peso por cocção. Houve efeito quadrático (P<0,05) sobre o teor de cinzas, perda de peso por descongelamento e oxidação lipídica para os filés armazenados em geladeira ou em freezer, sendo 33,75 ppm o nível de RAC que resultou em menor índice de oxidação lipídica. Conclui-se que a ractopamina a 11,5 ppm é efetiva em reduzir o teor de lipídeos em filés de pacu, embora aumente a formação de peróxidos em amostras mantidas em freezer após 60 dias. A 33,75 ppm a ractopamina é efetiva em reduzir os efeitos da oxidação durante o armazenamento em geladeira ou freezer.


Subject(s)
Animals , Food Quality , Lipids/analysis , Oxidation , Fishes/classification
19.
Biol. Res ; 47: 1-8, 2014. graf
Article in English | LILACS | ID: biblio-950770

ABSTRACT

BACKGROUND: Nitrosative and oxidative stress play a key role in obesity and diabetes-related mitochondrial dysfunction. The objective was to investigate the effect of curcumin treatment on state 3 and 4 oxygen consumption, nitric oxide (NO) synthesis, ATPase activity and lipid oxidation in mitochondria isolated from liver and kidneys of diabetic db/db mice. RESULTS: Hyperglycaemia increased oxygen consumption and decreased NO synthesis in liver mitochondria isolated from diabetic mice relative to the control mice. In kidney mitochondria, hyperglycaemia increased state 3 oxygen consumption and thiobarbituric acid-reactive substances (TBARS) levels in diabetic mice relative to control mice. Interestingly, treating db/db mice with curcumin improved or restored these parameters to normal levels; also curcumin increased liver mitochondrial ATPase activity in db/db mice relative to untreated db/db mice. CONCLUSIONS: These findings suggest that hyperglycaemia modifies oxygen consumption rate, NO synthesis and increases TBARS levels in mitochondria from the liver and kidneys of diabetic mice, whereas curcumin may have a protective role against these alterations.


Subject(s)
Animals , Male , Mice , Lipid Peroxidation/drug effects , Curcumin/pharmacology , Diabetes Mellitus, Type 2/diet therapy , Kidney/drug effects , Liver/drug effects , Mitochondria/drug effects , Oxygen Consumption/drug effects , Body Weight/drug effects , Mitochondria, Liver/drug effects , Mitochondria, Liver/enzymology , Adenosine Triphosphatases/drug effects , Oxidative Stress/drug effects , Cell Respiration/drug effects , Dietary Supplements , Diabetes Mellitus, Type 2/complications , Diabetes Mellitus, Type 2/physiopathology , Disease Models, Animal , Selective Breeding , Genotype , Hyperglycemia/diet therapy , Hyperglycemia/etiology , Mitochondria/enzymology , Nitric Oxide/analysis , Nitric Oxide/metabolism
20.
Nutrire Rev. Soc. Bras. Aliment. Nutr ; 38(3): 220-232, dez. 2013. tab
Article in Portuguese | LILACS | ID: lil-712164

ABSTRACT

The aim of this study was to determine the antioxidant activity of Moringa (Moringa oleifera Lam.) leaves flour in beef burger during storage for 120 days. Six hamburger formulations were processed: one control (without the use of additives), four with addition of Moringa leaves flour (0.10, 0.15, 0.20, and 0.25 g/100 g aggregate), and one with addition of synthetic antioxidant Propyl Gallate (0.01 g/100 g aggregate). The products were analyzed for their chemical composition with determinations of moisture, protein, dietary fiber, lipids, ash, carbohydrate, and caloric value after preparation. Microbiological and acceptance testing were performed at the beginning and after 120 days of storage. Determination of pH, instrumental color and lipid oxidation (TBARS) were performed at 1, 30, 60, 90 and 120 days of storage. All samples showed physical-chemical and microbiological tests in accordance with the Brazilian legislation. pH measurements were between 5.48 and 5.90; however, the intensity of red has changed according to the treatments and storage periods. The addition of Moringa leaves flour had no antioxidant effect on burgers, but its inclusion not only contributed to the improvement of nutritional quality, but also did not harm product acceptance.


El objetivo de este trabajo fue verificar la acción antioxidante de una harina de hojas de Moringa (Moringa oleífera Lam.) en carne de hamburguesa bovina durante su almacenamiento por 120 días. Se procesaron seis formulaciones de hamburguesa: una de control, sin uso de aditivos, cuatro adicionadas con harina de hojas de Moringa (0,10; 0,15; 0,20 y 0,25 g/100 g de masa) y una adicionada con antioxidante sintético Propil Galato (0,01 g/100 g de masa). Los productos se analizaron, en cuanto a su composición química, con determinaciones de humedad, proteína, fibra alimentaria, lípidos, cenizas, carbohidratos y valor calórico, y, tras la preparación, análisis microbiológicos y pruebas de aceptación, realizados tanto al inicio como al cabo de 120 días de almacenamiento. También se analizaron, con determinación de pH, Color instrumental y oxidación lipídica (TBARS), a los 1, 30, 60, 90 y 120 días de almacenamiento, respectivamente. Todas las muestras presentaron características físico-químicas y microbiológicas de acuerdo con la legislación nacional vigente. Los valores de pH oscilaron entre 5,48 y 5,90 y solamente la intensidad del rojo sufrió modificaciones debido a los tratamientos y periodos de almacenamiento. La adición de harina de hojas de Moringa no ejerció efecto antioxidante en las hamburguesas, sin embargo su inclusión contribuyó para mejorar la calidad nutricional y no perjudicó la aceptación del producto.


O objetivo deste trabalho foi verificar a ação antioxidante de uma farinha de folhas de Moringa (Moringa oleífera Lam.) em produto hambúrguer bovino durante estocagem por 120 dias. Foram processadas seis formulações de hambúrguer, sendo uma controle, sem o uso de aditivos, quatro adicionadas de farinha de folhas de Moringa (0,10; 0,15; 0,20 e 0,25 g/100 g de massa) e uma com adição de antioxidante sintético Propil Galato (0,01 g/100 g de massa). Os produtos foram analisados quanto à composição química, com determinações de umidade, pro¬teína, fibra alimentar, lipídeos, cinzas, carboidratos e valor calórico após o preparo, além de análises microbiológicas e teste de aceitação realizados no início e aos 120 dias de armazenamento, e determinação de pH, cor instrumental e oxidação lipídica (TBARS), realizadas com: 1, 30, 60, 90 e 120 dias de armazenamento. Todas as amostras apresentaram características físico-químicas e microbiológicas de acordo com a legislação nacional vigente. As medidas de pH ficaram compreendidas entre 5,48 e 5,90, e somente a intensidade de vermelho sofreu alterações de acordo com os tratamentos e períodos de estocagem. A adição de farinha das folhas de Moringa não exerceu efeito antioxidante nos hambúrgueres, porém sua inclusão contribuiu para a melhoria da qualidade nutricional e não prejudicou a aceitação do produto.


Subject(s)
Flour/analysis , Moringa oleifera/classification , Antioxidants , Malondialdehyde/analysis
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