ABSTRACT
Objective:To study the volatile oil of Notopterygium inchum and N. franchetii by using gas chromatography-mass spectrometry （GC-MS）. Method:Ten batches of N. inchum and N. franchetii were harvested from Sichuan and Gansu，including eight batches of wild samples， one batch of cultivated N. inchum and one batch of cultivated N. franchetii. Volatile oil was extracted according to the methods in Chinese Pharmacopeia， and the chemical constituents were analyzed with GC-MS technology. Result:Ninety-seven chemical compounds were identified，including monoterpenes，sesquiterpenes and non-terpenes. The calculation results of the relative content of chemical components showed that α-pinene（25.39%-41.68%），β-pinene（19.34%-40.95%） and limonene（4.59%-11.99%） were main compounds in volatile oil of N. inchum and N. franchetii. Relative content of α-pinene was higher than that of β-pinene in wild N. inchum and cultivated N. franchetii， while relative content of β-pinene was higher than that of α-pinene in wild N. franchetii. Relative content of β-pinene in samples stored for one year was lower than that of newly harvested ones. The partial least squares discriminant analysis with SIMCA-P+11.5 software showed that the samples from Gansu and Sichuan provinces had no significant differences， and additionally，one-year-stored samples and newly-harvested samples were clustered into two groups. Conclusion:This study showed that the storage had influence on the chemical constituents of volatile oil in N. inchum， and it also revealed the differences in volatile oil components of wild and cultivated Notopterygium. The above mentioned work provided chemical basis for the pharmacology comparison study of volatile oil from different kinds of N. inchum and N. franchetii.
ABSTRACT
Rhizoma et Radix Notopterygii is a rare and endangered Chinese medicine. In the collection of Notopterygium franchetii fruits, we collected a sample of N. forrestii , which is a spurious breed. Fruits of N. franchetii and N. forrestii are very similar in morphology and can be easily confused. Until now the morphological identification of the fruits of Notopterygium has not been reported. To provide a scientific basis for the identification of N. franchetii and N. forrestii fruits, the morphology and microscopic identification were studied in this paper. In this study, stereomicroscope and paraffin sections were used to compare the morphological characteristics and microscopic characteristics of these two fruits. Our results showed that these two fruits were different in size, surface texture and the number of vertical edges on the back. These traits can be used as diagnostic characteristic of these two fruits. The difference between the number of tubing and the endosperm cell contents can be used as microscopic identification features. The above discriminative characteristics can distinguish the two fruits and provide scientific basis for the identification and germplasm evaluation of Notopterygium fruits.