ABSTRACT
Objective:To construct a C57BL/6 mouse model of simulating transurethral thulium laser vaporization prostatectomy.Methods:Twelve male C57BL/6 mice were selected to undergo transvesical vaporization resection of the urothelium covering the urethra of the prostate using thulium laser. The urethral tissue of the prostate was retrieved on the 1st, 3rd, 5th, and 7th days after the surgery. HE staining was used to observe the process of re-epithelialization of the urethral wound of the prostate. Immunohistochemical (IHC) staining was used to detect whether the re-epithelialized cells of the urethral wound of the prostate expressed urothelin Ⅲ (UPⅢ).Results:On the first day after surgery, HE staining showed complete destruction to the urothelium covering the urethra of the prostate, with a large amount of coagulative necrotic tissue on the wound surface, and IHC staining showed no expression of UPⅢ on the wound surface. On the 3rd day after surgery, HE staining showed that there were still no regenerated epithelial cells on the wound surface, with coagulation necrosis tissue significantly reduced, and the urethral cavity was clearly visible. And IHC staining showed no expression of UPⅢ on the wound surface. On the 5th day after surgery, HE staining showed 1-2 layers of regenerated epithelial cells lacking cell polarity on the wound surface, and IHC staining showed that the regenerated epithelial cells expressed UPⅢ. On the 7th day after surgery, HE staining showed 4-6 layers of polar regenerated epithelial cells on the wound surface, and IHC staining showed the multiple layers of regenerated epithelial cells expressing UPⅢ.Conclusions:Based on the simulation of transurethral thulium laser vaporization resection of the prostate, the thulium laser and ultra micro endoscope system were used to vaporize the urothelium covering the urethra of the prostate, and the process of urethral re-epithelialization of the prostate can be observed after surgery. The establishment of the C57BL/6 mouse model simulating thulium laser vaporization prostatectomy provides a new research platform for studying the mechanism of wound repair after prostatectomy.
ABSTRACT
Objeodve To discuss the safety and efficacy of " point-line-surface" three step method of straight light beam green laser photoselective vaporesection of the prostate(PVRP) for the treatment of benign prostatic hyperplasia(BPH).Methods The data of one hundred and twenty-six BPH patients who were treated with surgery in the First Affiliated Hospital of Xinxiang Medical University from May 2016 to August 2017 was analyzed retrospective.In all of the patients,69 cases were given "point-line-surface" three step method of straight light beam green laser PVRP(PVRP group),57 cases were given photoselective vaporization of the prostate (PVP group).Operation time,blood loss,postoperative washing time,indwelling catheter time,international prostate symptom score (IPSS),quality of life score (QOL),maximum urinary flow rate (Qmax) and postvoid residual urine (PVR) were compared between the two groups.Results The operation time in the PVRP group was shorter than that in the PVP group(P ＜ 0.05).There was no significant difference in the blood loss,postoperative washing time and indwelling catheter time between the PVRP group and PVP group(P ＜ 0.05).There was no significant difference in the IPSS,QOL,Qmax and PVR between the PVRP group and PVP group before operation (P ＜ 0.05).In the two groups,the IPSS,QOL and PVR were lower after operation than that before operation (P ＜ 0.05),while Qmax was higher after operation than that before operation(P ＜ 0.05).There was no significant difference in the IPSS,QOL,Qmax and PVR between the PVRP group and PVP group after operation(P ＜ 0.05).The rate of postoperative complications in the PVRP group and PVP group was 1.4％(1/69) and 1.8％ (1/57),respectively;there was no significant difference in the rate of postoperative complications between the PVRP group and PVP group (x2 =11.968,P ＜ 0.05).Conclusion " Point-line-surface" three steps of straight light of PVRP for treating BPH have simple steps and short operation time.It is a safe and ideal surgical method for BPH.