Analysis of standard decoction of salt-processed Psoraleae Fructus / 中国中药杂志

The traditional Chinese medicine standard decoction is prepared on the basis of the theory of traditional Chinese medicine and clinical application. With reference to the modern extraction method,the single decoction of traditional Chinese medicine is prepared by the standardized process,and the establishment of its quality standards is conducive to standardizing clinical medication. This research is to set an evaluation standard for the quality of salt-processed Psoraleae Fructus standard decoction. Twelve batches of salt-processed Psoraleae Fructus standard decoctions were prepared. The contents of psoralen and isopsoralen were determined,the transfer and extract rates were calculated,and the pH value was measured; HPLC fingerprint method was established for analysis. The results of the 12 batches of samples revealed that the transfer rates of psoralen and isopsoralen were 17. 10%-26. 40%,14. 70%-22. 70%,respectively; the extract rate was between 14. 7%-27. 0%,and the pH value was between 5. 4-6. 9. Moreover,7 common chromatographic peaks were determined based on fingerprint by using similarity evaluation system for chromatographic fingerprint of traditional Chinese medicine( 2012 A).The similarities of the 12 batches of samples were analyzed and compared,and the results showed that the similarities were all higher than0. 9. In this study,the preparation method for salt-processed Psoraleae Fructus decoction was standard,with high similarities in fingerprint. This study build a convenient and reliable method of comprehensive quality evaluation,with a high precision,stability and repeatability,which can provide a reference for the quality control of salt-processed Psoraleae Fructus dispensing granules.

Quality evaluation system for standard decoction of salt-processed Psoraleae Fructus / 中草药

Objective To establish a quality evaluation system for standard decoction of salt-processed Psoraleae Fructus (PF). Methods Fifteen batches of crude drugs were collected and processed into decoction pieces with salt complied with the 2015 Edition of the Chinese Pharmacopoeia, and then the standard decoctions of salt-processed PF were prepared as freeze-dried powders. Based on the established HPLC fingerprint, seven common peaks were recognized and the main chemical constituents were identified in combination with time-of-flight mass spectrometry. Results Benzofuran glycosides and furanocoumarins turned out to be the main composition of standard decoction of salt-processed PF. The dry extract yielding rate varied from 16.31% to 20.59%, while the total contents of psoralen and isopsoralen were 1.17%—1.50% with a transfer rate ranging from 13.55% to 23.57%, and the fingerprint similarity of 15 batches of samples were all higher than 0.9. Conclusion This stable and reliable method of comprehensive quality evaluation for standard decoction of salt-processed PF may provide reference for quality control of salt-processed PF dispensing granules and related preparations.