ABSTRACT
OBJECTIVE: To study the chemical constituents of the antioxidant active sites and establish the determination method of the total content of phenolic acid in Actinidia chinensis Planch. seeds. METHODS: AB-8 macroporous adsorption resin, silica gel and ultra performance liquid chromatography-mass spectrometry (UPLC-MS/MS) were used to separate and purify the compounds, and the compounds were identified according to the properties and spectral data of the compounds. The total content of phenolic acid was determined by the spectrophotometry. To establish a method for determination of total content of phenolic acid using ferulic acid as a positive control and ferric chloride-potassium ferricyanide solution as a color developer and methodological investigation was carried out. RESULTS: Twelve compounds were isolated and identified from the active parts of Actinidia chinensis Planch. seeds, which were protocatechuic acid (1), vanillic acid (2), syringic acid (3), protocatechuic aldehyde (4), 3,4-dihydroxyace to phenone. ethyl ketone (5), dihydroconiferyl alcohol (6), 4, 4-dihydroxydiphenylmethane (7), shikimic acid (8), ferulic acid (9), 3-acetoxymethyl-4-methoxy methyl benzoate (10), bis(4-methoxyphenyl) methane (11), and methyl caffeate (12). The total content of phenolic acid in kiwifruit seeds was determined by ultraviolet spectrophotometry. The linear range of the method was 1.6-16 μg•mL-1. The regression equation is Y=0.087 8x+0.045 8, r2=0.999 3, the average recovery is 99.36%, and the RSD is 2.869 9%.The total content of phenolic acid in the total extract was 6.61%, and the active site was 42.46%. The total content of phenolic acid in the active site is about 7 times that of the total extract. It can be seen from the results that the extraction process can effectively enrich the phenolic acid compounds. CONCLUSION: Compounds 5-7 and 10-12 are isolated from the plant for the first time. The determination method of total content of phenolic acid is simple, fast, stable and reproducible, which provides a scientific basis for the systematic study of phenolic acid compounds in kiwifruit seeds.
ABSTRACT
Objective:To compare the quality differences in benzalkonium chloride samples from domestic and abroad to provide references for the quality standard revision for benzalkonium chloride in Chinese pharmacopoeia. Methods: The ratio of alkyl compo-nents was determined according to the method described in USP 38, and the total content of benzalkonium chloride was determined ac-cording to the method recorded in Chinese Pharmacopoeia (2015 edition) and USP 38, respectively. Results:According to the results of composition ratio of alkyl, the fraction defective of domestic samples and imported samples was 100% and 50%, respectively. The content difference between the values calculated by the methods in the two pharmacopoeias showed that the total content of domestic samples changed from 3. 86% to 4. 15%, and that of imported samples changed from 1. 15% to 3. 90%. Conclusion:There are sig-nificant differences in the quality of benzalkonium chloride between domestic samples and imported samples. It is recommended that the ratio of alkyl components should be supplemented in our pharmacopoeia referring to the method in USP 38 and the total content calcula-tion formula for benzalkonium chloride should be revised to improve the quality standard for benzalkonium chloride.