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Braz. j. microbiol ; Braz. j. microbiol;44(3): 901-904, July-Sept. 2013. tab
Article in English | LILACS | ID: lil-699786

ABSTRACT

The aims of this study were to standard and optimize a qPCR protocol with FAM-BHQ1 probe, and to compare its sensitivity against TaqMan qPCR and PCR methods to diagnose shrimp WSD. The FAM-BHQ1 qPCR presented higher clinical sensitivity and showed to be a robust alternative to detect WSSV in clinical samples.


Subject(s)
Animals , Molecular Diagnostic Techniques/methods , Penaeidae/virology , Real-Time Polymerase Chain Reaction/methods , Veterinary Medicine/methods , Sensitivity and Specificity
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