Expression of JAK2/STAT3 signaling pathway in human umbilical vein endothelial cells exposed to the serum of type 2 diabetic macroangiopathy patients / 第二军医大学学报

Objective To investigate the role of Janus kinase 2/signal transducer and activator of transcription 3(JAK2/STAT3) signaling pathway in the pathogenesis of type 2 diabetic macroangiopathy. Methods The human umbilical vein endothelial cells (HUVECs) were incubated for 24 h with the serum of healthy volunteers, simple type 2 diabetic patients and patients with type 2 diabetic macroangiopathy. JAK2 specific inhibitor AG490 was used to block the JAK2/STAT3 signaling pathway. According to different treatments, the cells were divided into normal control group (NC group, n=30), simple diabetes mellitus group (DM group, n=30), type 2 diabetic macroangiopathy group (DV group, n=30), DM+AG490 group (DM+AG490 group, n=30) and DV+AG490 group (DV+AG490 group, n=30). Real-time quantitative PCR technique was used to detect the mRNA expression of JAK2, STAT3, vascular endothelial growth factor (VEGF) and vascular endothelial growth factor receptor (FLT1) in each group. Western blotting analysis was used to detect the protein expression of JAK2, STAT3 and phosphorylated STAT3(p-STAT3). Results Compared with the NC group, the expression of JAK2, STAT3 mRNA and JAK2, and p-STAT3 protein were significantly up-regulated in DM and DV groups (P<0.05), and the expression of JAK2, STAT3 mRNA and JAK2, p-STAT3 protein in DV groups were significantly higher than those in DM group (P< 0.05). The expression of JAK2, STAT3 mRNA and JAK2, p-STAT3 protein in DM+AG490 group and DV+AG490 group were significantly lower than those in the DM group and DV group (P< 0.05). Compared with the NC and DM group, the expression of VEGF, FLT1 mRNA was significantly up-regulated in DV group(P<0.05). Compared with the DV group, the expression of VEGF and FLT1mRNA were significantly reduced in DV+AG490 group (P< 0.05). Conclusion JAK2/STAT3 signaling pathway may play a role in the pathogenesis of type 2 diabetic macroangiopathy.

Relationship between C-peptide and artherosclerosis in lower extremity in patients with type 2 diabetes / 实用医学杂志

Objective To investigate the relationship between C-peptide and artherosclerosis in lower ex-tremity in patients with type 2 diabetes. Methods Two hundred and fourteen type 2 diabetic patients were included in this retrospective study. They were divided into two groups:type 2 diabetes with artherosclerosis in lower extremi-ty in 123 cases and type 2 diabetes without artherosclerosis in lower extremity in 91 cases. Their medical history and the laboratory data were collected such as fasting serum C-peptide levels and postprandial serum C-peptide levels of 1 hour, 2 hours and 3 hours, glycated hemoglobin (HbA1c) and so on. Results Compared with artherosclerosis group in lower extremity, the age, duration of diabetes, total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), glycated hemoglobin and smoking rate were significantly higher in type 2 diabetes without artherosclero-sis in lower extremity (P 0.05). Logistic regression analysis showed that fasting and postprandial (1 hour, 2 hours and 3 hours)serum C-pep-tide levels were all negatively related to artherosclerosis in lower extremity (OR 0.524,P = 0; OR 0.440, P = 0;OR 0.688, P=0;OR 0.795, P=0). Conclusions Serum C-peptide level may be an independent associated fac-tor with artherosclerosis in lower extremity in type 2 diabetes.

Effects of statin reloading before percutaneous coronary intervention on circulatory endothelial progenitor cells and inflammatory cytokines / 中国病理生理杂志

AIM:To investigate the effects of atorvastatin reloading in pre-percutaneous coronary intervention ( PCI) period on endothelial progenitor cell ( EPC) count and inflammatory cytokine expression in the stable angina pectoris patients who had previously received long-term statin treatment.METHODS:The patients with stable angina pectoris that had received long-term statin therapy and planned to accept PCI were randomized into 3 groups:80 mg atorvastatin 12 h and 40 mg 2 h before coronary angioplasty (80 mg reloading), pre-operatively with 40 mg/d atorvastatin for 7 d (40 mg re-loading) , and without atorvastatin reloading ( no reloading ) .CD45 -/CD133+/CD34 +, CD45 -/CD34 +/KDR+ and CD45 -/CD144 +/KDR+EPCs in 100 μL peripheral blood were determined by flow cytometry 1 h prior to PCI and 1 h, 6 h and 24 h after PCI.The serum concentrations of soluble intercellular adhesion molecule 1 ( sICAM-1) , C-reactive protein ( CRP) and troponin I ( TnI) were analyzed immediately prior to and 24 h after PCI.RESULTS:(1) In 80 mg reloading group, the numbers of circulating CD45 -/CD133 +/CD34 +and CD45 -/CD34 +/KDR+early differentiation stage EPCs 1 h and 6 h after coronary angioplasty was significantly elevated compared with those before PCI (P<0.05).(2) In control group, the serum concentrations of sICAM-1 and CRP 24 h after PCI were significantly elevated ( P<0.05) compared with preoperative values.(3) The rise in serum TnI concentration from pre-to post-operation in 80 mg reloading group was lowerthan that in control group.CONCLUSION: The method of atorvastatin reload before PCI affects the number of EPCs inperi-operative period.High dose of atorvastatin application before PCI triggers early EPC circulation.The serum levels ofpost-operative inflammatory cytokine sICAM-1 as well as CRP are reduced by atorvastatin reloading before PCI.

Clinical effect of amlodipine atorvastatin calcium in the treatment of hypertensive patients with dyslipidemia / 中国基层医药

Objective To investigate the treatment effect of amlodipine atorvastatin calcium for hypertensive patients with dyslipidemia.Methods The clinical data of 200 hypertensive patients with dyslipidemia were retrospectively studied.They were divided into the control group and the intervention group according to treatment method,100 cases in each group.The control group was given amlodipine besylate based on the conventional treatment,the intervention group was treated with amlodipine besylate and atorvastatin calcium tablets.The clinical effect was observed and compared between the two groups.Results After treatment,the systolic and diastolic blood pressure of the two groups were significantly lower than before treatment (t =4.57,5.32,4.72,4.61,all P ＜ 0.05) ; After treatment,IMT and plaque area of the two groups were obviously lower than before treatment(t =3.16,3.43,all P ＜ 0.05) ;After treatment,IMT and plaque area of the intervention group were (0.71 ± 0.29) mm and (1 t.13 ± 3.61) mm2,which were significantly lower than those of the control group [(0.82 ± 0.26) mm and (13.64 ± 2.86) mm2] (t =3.28,4.05,all P ＜ 0.05).Conclusion Amlodipine besylate and atorvastatin calcium tablets can effectively reduce hypertension,which has good synergistic effect and protective effect on blood vessels.

Factores de riesgo de la cardiopatía isquémica / Risk factors in ischemic heart disease

Introducción: la enfermedad arterial coronaria es una de las principales causas de mortalidad en todo el mundo. En su fisiopatología juega un papel importante el desarrollo y la progresión de la aterosclerosis coronaria, la cual está íntimamente relacionada con determinados hábitos de vida y ciertas características personales que se conocen como factores de riesgo. Objetivo: actualizar a los profesionales de la Atención Primaria de Salud sobre los factores de riesgo de la Cardiopatía Isquémica. Métodos: se realizó una revisión bibliográfica en libros de Medicina y artículos científicos disponibles en revistas digitales, a través de buscadores de información. Se accedió a diferentes fuentes de información como bases de datos, libros electrónicos, revistas electrónicas, etcétera, de infomed e internet. Luego se hizo un análisis crítico sobre el tema, avalado por la en contrado en la literatura consultada. Desarrollo: la situación de las enfermedades cardiovascularesen el mundo ha pasado por distintas fases, en correspondencia con el desarrollo socioeconómico de los países y el aumento en la incidencia de los distintos factores de riesgo, los cuales influyen de manera diferente en la aparición de la Cardiopatía Isquémica. Conclusiones: es importante conocer la influencia de cada factor de riesgo cardiovascular en la aparición de la Cardiopatía Isquémica para tomar estrategias encaminadas al control de los mismos y evitar llegar a la enfermedad...

Introduction: coronary artery disease is one of the main causes of mortality worldwide. An important role in the physiopathology of this disease is played by the development and progression of coronary artherosclerosis which is closely related to certain life habits and individual characteristics known as risk factors. Objective: to provide the primary health care professionals with an updating on the risk factors of ischemic heart disease. Method: a literature review including medical books and scientific articles from on line journals was made through the use of information searchers. It was possible to have access to several sources of information such as databases, electronic books, electronic journals, etc from Infomed and Internet. Finally, a critical analysis of the topic, supported on the findings of this literature, was made. Discussion: the situation of cardiovascular diseases worldwide has undergone several phases according to the social and economic development of the nations and the increase of the incidence of a number of factors affecting in different ways the occurrence of ischemic heart disease. Conclusions: it is important to know about the influence of each cardiovascular risk factor over the occurrence of ischemic heart disease in order to draw strategies for their control and to prevent the disease...

Influence of apolipoprotein E polymorphism in onset of hypertensive intracerebral hemorrhage / 中华神经医学杂志

Objective To investigate the relationship between apolipoprotein E (APOE) genotype and onset of hypertensive intracerebral hemorrhage (HICH). Methods One hundred and twenty-eight patients with HICH,admitted to our hospital from September 2007 to December 2010,and 84 healthy controls clinically flee from neurology disease were chosen in our study.A POE genotype was determined using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP); the distribution ofAPOE genotype and allele fiequency between the 2 groups were compared; the age of onset,gender,blood pressure,blood fat,bleeding part and size of haematoma in patients with different APOE genotype were further studied. Results The subjects with smoking hobby,diabetes history and higher concentrations of TC and LDL-C in the HICH group were obviously more than those in the control group (P＜0.05).As compared with those in the controls,the distributions of the APOEε3/4 and APOEε4 in the HICH group were significantly higher, and the distributions of the APOEε3/3 and APOEε3 in the HICH group were significantly lower (P＜0.05). The APOEε3/4 and APOEε4 carriers enjoyed higher percentages of onset age less than 45 years and superficial haematoma than A POEε3/3 and the A POEε3 carriers. The onset systolic blood pressure in APOEε3/4 and APOEε4 carriers was obviously lower than that in A POEε3/3 and A POEε3 carriers (P＜0.05). The plasma concentrations of LDL-C and TC in APOEε3/4 and APOEε4 carriers was significantly higher than those in APOEε3/3 and APOEε3 carriers (P＜0.05). Conclusion The APOE genotype influences the onset of HICH, and its influence factors included onset age, blood pressure,blood fat and bleeding part,which may concern with the cerebral artherosclerosis or amyloidosis.

Relationship between cerebral infarction combined with type 2 diabetes and carotid atherosclerosis / 中华神经医学杂志

Objective To investigate the relationship between cerebral infarction combined with type 2 diabetes and carotid atherosclerosis. Methods One hundred and thirty-three patients with diabetes complicated with cerebral infarction and 150 patients with type 2 diabetes (controls),admitted to our hospital from January 2010 to December 2011,were chosen in our study.The levels of homocysteine (Hcy) and triglyceride (TG) were detected with automatic biochemical analyzer; cholesterol oxidase method was employed to measure the levels of total cholesterol (TC),high-density lipoprotein (HDL) and low-density lipoprotein (LDL); immunoturbidimetric assay was used to detect the level of fibrinogen (FIB) and the formation ofartherosclerotic plaque. Results The levels of c-reactive protein,Hcy and LDL in the observation group were significantly higher than those in the control group (P＜0.05).The HDL level in the observation group (1.11±0.28 mmol/L) was obviously lower than that in the control group (1.53±0.37 mml/L,P＜0.05).The detection rate ofartherosclerotic plaque in observation group (54.5％) was significantly higher than that in the control group (21.3％,P＜0.05). Conclusion A close relationship between carotid atherosclerosis and cerebral infarction complicated with type 2 diabetes is noted.

Human cytomegalovirus infection concerned with carotid atherosclerosis / 中国综合临床

Objective To study the correlation of human cytomegalovirus infection with carotid atherosclerosis.Methods A total of 120 patients with carotid atherosclerosis and 140 healthy control patients were recruited for HCMV-PP65 antigen detection and Ultrasound examination.Results In carotid atherosclerosis and healthy patients,58.20％(71 cases)and 6.43％(9 cases)of the subjects were positive for HCMV-PP65 antigen(x2 =32.98,P ＜ 0.05).In carotid atherosclerosis group,69.01％(49 cases)of the patients with positive HCMV-PP65 antigen had instable plaques,while it was 47.06％(24 cases)in the patients with negative HCMV-PP65 antigen.The difference in the positivity of HCMV-PP65 between the two groups were significant(x2 =8.22,P ＜ 0.05).Conclusion Active infection of HCMV may be associated with Carotid Atherosclerosis and the plaques will be more instable.

Expression of CD40 and matrix metalloproteinase 9 in carotid atherosclerotic plaques and its significance / 第二军医大学学报

Objective: To investigate the expression of CD40 and MMP9 in carotid atherosclerotic plaques, so as to assess the role of CD40 in the stability of the plaque and the possible mechanism. Methods: The expression of CD40 and MMP9 mRNA and protein in carotid atherosclerotic plaques obtained from carotid eversion endarterectomy (CEE) of 28 patients with high-grade stenosis (>70%) (stroke group, n=15; non-stroke group, n=13) and 8 normal postmortem arteries (control group) were detected by real-time quantification polymerase chain reaction (PCR) and Western blotting. The correlation between expression of CD40 and MMP9 was analyzed. Results: The expression of CD40 mRNA and MMP9 mRNA in the non-stroke group and stroke group was significantly higher than that in control group (P<0.01); and that of the stroke group was significantly higher than that of the non-stroke group (P<0.01). There was a linear correlation between expression of CD40 and MMP9 mRNA (r=0.964, P<0.01). Extremely rare expression of CD40 and MMP9 protein wet found in the normal carotid artery; the protein expression was evidently higher in the carotid artery atherosclerosis than in the normal carotid artery, and the expression in carotid artery atherosclerosis in the stroke group was higher than that in the non-stroke group. Conclusion: The increased expression of CD40 and MMP9 in the carotid atherosclerositic plaques may be closely related to the stability of plaques.

Moxibustion with Medicine Cake on mRNA Expression of Matrix Metalloproteinases-2, 9 in Artherosclerosis Plaques of Rabbits / 中国康复理论与实践

@#ObjectiveTo observe the effect of moxibustion with medicine cake on mRNA expression of matrix metalloproteinases-2,9 (MMP-2，MMP-9)in artherosclerosis (AS) plaques of rabbits, and to explore the effect of moxibustion with medicine cake to the stability of artherosclerosis plaques of rabbits.MethodsAS rabbit models were established by feeding high-fat diet. 75 New Zealand big-eared rabbits were divided into 5 groups, blank group (blank control group), model group (AS model group), direct moxibustion group (AS model + moxa cone direct moxibustion), moxibustion on medicine cake group (AS model + moxibustion on medicine cake), western medicine group (AS model + atorvastatin), 15 rabbits in each group. Then the mRNA expression of MMP-2, MMP-9 was detected in the atherosclerotic plaques of rabbits with in situ hybridization.ResultsThe expressions of MMP-2, MMP-9 in the atherosclerotic plaques of rabbits were all be controlled in direct moxibustion group, moxibustion on medicine cake group, western medicine group (P<0.01 or P<0.05). The expressions of MMP-2 and MMP-9 in the moxibustion on medicine cake group and western medicine group were obviously lower than those of the direct moxibustion group.ConclusionThe expression of MMP-2, MMP-9 in the atherosclerotic plaques of rabbits can all be controlled in direct moxibustion group, moxibustion on medicine cake group, and western medicine group; as well as the plaques can all be made stable. The efficacy in the moxibustion on medicine cake group and western medicine group is superior to that of the direct moxibustion group.

Effects of Tongxinluo capsule on endodermis function and the expression of CD40, CD40L in artherosclerosis rabbits / 中国基层医药

Objective To investigate the therapeutic effects of Tongxinluo and its influence on the expression of CD40 and CD40L in artherosclerosis rabbits. Methods 60 male rabbits were divided into three groups randomly,20 of each group: normal control group, model group and Tongxinluo group. The rabbits in model group and Tongxinluogroup were used to prepare the atherosclerosis model induced by high-cholesterol diet. The levels of ET-1, NOwere measurde. Before and 12 weeks after durg treatment,serum total cholesterol ,plaque areas and ratios of intima/media thickness were detected. The expression of CD40 and CD40L mRNA were determinated by quantitive RT-PCR. Results Serum total cholesterol level of Tongxinluo group was decreased compared with model group ( P < 0. 05 ), and they were both much higher than those of normal control group ( P < 0. 01 ); as compared with the model group, aortic plaque areas and ratios of intima/media thickness in Tongxinluo group reduced significantly [ (0.56 ± 0. 07) vs ( 1.16±0.08),P<0.01;(36.88±2.38)% vs (76.58 ±2.86) %,P <0. 0l] ;The expression of CD40 and CD40L mRNA also decreased in Tongxinluo group with statistic significance[ (0.798 ± 0.115 )、 (0. 592 ± 0. 132) vs (0.686±0. 132) 、(0.498 ±0. 108) ,P <0. 01 ) ]. Conclusion Tongxinluo had the ability to anti-artherosclerosis and its possible mechanism was down-regulate the expression of CD40 and CD40L.

Effects of rosiglitazone on the expression of nuclear factor-κB P65 and metalloproteinase-9 mRNA in peripheral blood monocyte-derived macrophages in patients with coronary artery disease / 中华急诊医学杂志

Objective To investigate the effects and mechanisms of rosiglitazone on the expressions of nuclear factor-κB and matrix metalloprotease (MMP-9) in peripheral blood monocyte-derived macrophages (MDMs) in patients with coronary heart disease. Method This was a clinical case-control study. Forty-eight actue coronary symdrome (ACS) patients (ACS group), and 20 patients with stable angina (SA) (control group) were collected. They were performed coronary arteriography in the Department of Cardiology of the Second Xiangya Hospital from March to April in 2007. Exclusion criteria included acute infection, trauma or surgery patients within four weeks, cerebral vascular accident, liver and kidney dysfunction, cancer, and so on. The peripheral blood mononuclear cells were isolated and transformed into MDMs with macrophage colony-stimulating factor treatment. The transformed MDMs were randomly assigned into subgrougs and incubated with 0 /μmol/L, 1 μmol/L, 10 μmol/L, 20 μmol/L of rosiglitazone respectively. The expressions of PPAR-γ mRNA, MMP-9 mRNA were determined by RT-PCR and nuclear factor-κB P65 (NF-KB P65) expression by immunohistochemistry. Multiple comparisons were examined for significant differences using analysis of variance (ANOVA). Results The basal expression of PPAR-y mRNA was lower, in contrast, the levels of NF-KB P65 and MMP-9 mRNA were higher in ACS group than control group. PPAR-γ mRNA expression were significantly upregulated in both ACS and control groups with rosiglitazone treatment. PPAR-γ mRNA expression was positive correlation, while the expressions of MMP-9 mRNA were negative correlation with the rosiglitazone concentration in the ACS group. Rosiglitazone inhibited the expression of NF-KB in a concentration-independent manner in ACS and control groups. Conclusions The expression of PPAR-y mRNA is inhibited, while the activity of NF-KB and expression of MMP-9 mRNA are enhanced in MDMs of ACS cases. Rosiglitazone intervention may inhibit NF-KB activity and MMP-9 expression by upregulation of PPAR-y expression in MDMS of patiens with ACS.

Using the combination of traditional risk factors and quantitative coronary angiography (QCA )in predicting the risk of individuals with subclinical artherosclerosis / 中华流行病学杂志

Objective To determine whether the combination of traditional risk factors and quantitative coronary angiography (QCA) assessment could provide accurate prognostic information on a population-based study including 1137 adults with subclinical artherosclerosis and with coronary risk factors. Methods Participants underwent coronary angiography examination before the minimal stenotic diameters, segment diameters, percent stenosis, plaque areas. Other parameters were analyzed by the computer-assisted Coronary Angiography Analysis System. The Framingham Risk Score for each participant was assessed. During the 1 year follow-up period, all kinds of endpoint cardiovascular events were screened. Endpoint events were defined as death from coronary heart disease, nonfatal myocardial infarction (MI) or unstable angina pectoris. Results During the 1 year of follow-up period, a total of 124 participants developed an endpoint event, which was significantly associated with the Framingham Risk Score, calcium of plaques and the plaque areas (all Ps＜0.05).The QCA score incorporated with the QCA parameters was related to the endpoint events. The Framingham Risk Score was combined with QCA score through logistic regression for prediction of end-point events. Data from the ROC analysis showed the accuracy of this prediction algorithm was superior to the accuracy when variables themselves were used. The event-free survival rate was inferior to the control group in participates under high risk, when being screened with this prediction algorithm (P＜0.05). Conclusion The risk of cardiovascular attack in subclinical artherosclerosis individual seemed to be associated with the Framingham Risk Score, calcium of plaques and the plaque areas. When the traditional risk factors (the Framingham Risk Score) were combined with QCA, the new method could provide more prognostic information on those adults with subclinical artherosclerosis.

The expression of resistin-like molecules-a in atherosclerotic plaque / 中华急诊医学杂志

Objective To explore the expression of resistin-like molecules-a (RELMa) in atherosclerotic plaque of ApoE-/- mouse, and to study the effects of RELMa on the proliferation and migration of vascular smooth musclee cells (VSMCs) . Method Nine ApoE-/- mice and nine C57BL/6J mice were fed with high fat diet. All mice were sacrificed 24 weeks after force feeding. Vessels were dissected from to abdominal aorta. Sections of aortic tissue were stained with HE dyeing and RELMa in aortic tissue was assayed by immunohistochemistry. The expression of RELMa mRNA in vessels was detected by RT-PCR. The effects of different concentration RELMa in different concentrations on the proliferation and migration of VSMCs were detected. Data was expressed as mean ± standard deviation. ANOVA were used for comparison in SPSS 11.0, and changes were considered as statistically significant if P value was less than 0.05. Results Atherosclerosis plaque formed in aortic root of ApoE-/- mice after they were fed with high fat diet for 24 weeks. RELMa protein and RELMa mRNA were found by immunohistochemistry and RT-PCR in atherosclerotic plaque of ApoE-/- mouse. RELMa protein didn't be found in vessels of control mouse. RELMa promoted the proliferation of VSMCs (RELMa groups: 2811. 21 ± 216. 89,4056. 87 ±220.65,5061.45 ± 335.86, vs. control 1609.58 ± 203.53, P < 0.01). RELMa promoted the migration of VSMCs (RELMa groups: 130.54±12.98,158.39±11.58,203.50± 17.37 vs. control:70.54± 11.92, P<0.01).Conclusions RELMa expresses in atherosclerotic plaque of ApoE-/- mouse. RELMa enhances the proliferation and migration of VSMCs of aorta.

Correlation of serum interleukin-6 and interleukin-18 levels to carotid artery intimal-medial thickness in patients with acute cerebral infarction / 中华神经医学杂志

Objective To investigate the correlation of serum interleukin-6 (IL-6) and IL-18levels to carotid artery intimal-medial thickness (IMT) in patients with acute cerebral infarction (ACI).Methods In 64 patients with ACI and 30 healthy volunteers, serum IL-6 and IL-18 levels were detectedusing radioimmunoassay and enzyme-linked immunosorbent assay (ELISA), respectively. Carotid arteryIMT were measured in the two groups using color Doppler ultrasound, and the ACI patients were dividedinto two groups with carotid IMT greater or lower than 1.0 mm. Results The ACI patients and thehealthy volunteers showed significant differences in serum IL-6 (P<0.05) and IL-18 levels (P<0.05),which were also significantly different between ACI patients with carotid IMT ≥ 1.0 mm and those withIMT<1.0 mm (P<0.05). The ACI patients with IMT<1.0 mm had comparable serum IL-6 and IL-18 levelsto those in the healthy volunteers. In patients with carotid IMT ≥ 1.0 mm, the serum IL-18 level waspositively correlated to IMT (r=0.549, P=0.001), but the IL-6 level was not related to IMT (P>0.05).Conclusion The serum levels of IL-6 and IL-18 are elevated in ACI patients with increased carotid IMT.

Efecto de la vitamina C, sobre la actividad de glutation peroxidasa y la formación de ateromas, en conejos expuestos a dieta hiperlipidémica / Effects vitamin C, on glutathione peroxidase activity and atheroma formation, in rabbits exposed to a hyperlipidaemic diet

El estrés oxidativo juega un papel muy importante en la aterosclerosis; de hecho existe evidencias que indican que los antioxidantes son moléculas capaces de retardar y/o revertir el proceso aterosclerótico. El objetivo del presente estudio fue comparar el efecto de la vitamina C (Vit C), sobre la actividad de la Glutation peroxidasa (GPx) y la formación de ateromas en conejos. Se estudiaron 36 conejos divididos en 3 grupos: Grupo 1 (Control): conejarina, Grupo 2: huevo y conejarina, Grupo 3: huevo, conejarina y Vit C (100mg/diarios). el período experimental duró 12 semanas. Se determinó perfil lipídico por métodos enzimáticos y la actividad de GPx por cinética en 0 y 12va semana. Los conejos fueron sacrificados y se les realizó estudio histológico de su aorta. Los resultados revelaron un incremento en la actividad de la GPx en los grupos 2 y 3 con respecto al control en la 12va semana de experimentación (p<0,05). Hubo inhibición de lesiones ateroscleróticas en los conejos del grupo 3. En conclusión en condiciones de hiperlipidemia con o sin suplementación de Vit C, existe incremento en la actividad de GPx. Por otra parte, la Vit C disminuye y evita la progresión de ateromas.

Oxidative stress plays an important role in artherosclerosis; so antioxidants are molecules have been used to slow down or inihibit atherosclerosis. The objetive of the presents study was to compare the effect of Vitamin C (Vit C), on serum Glutathione peroxidase activity (GPx) and on the formation of aortic lesions in rabbits. 36 rabbits were studied: Group 1: "conejarina" (commercial rrabit food); Group 2: egg and conejarina, Group 3: egg, conejarina and Vit C (100mg/day). The experimental lasted 12 weeks. Lipid profile was done by enzymatic methods and GPx by kinetic method in weeks 0 and 12. Histological study of rabbit's aorta was done. GPx activity in groups 2 and 3, increased compared with controls, from weeks 12 of experimentation (o<0,05). There was inihition of aortic lesions in groups 3. In conclusion, under hyperlipidemic conditions, with or without Vit C supplementation, activity of GPx there is increase. Vit C reduces and prevents the progression of atheromas.

Association of metabolic syndrome with the pulse wave velocity / 대한내과학회지

BACKGROUND: Atherosclerotic cardiovascular disease and metabolic syndrome are both rapidly increasing in Koreans due to the new westernized eating habits and the aging of the population. The pulse wave velocity (PWV) reflects arterial stiffness and it may be used as an indicator of atherosclerosis. This study was conducted to investigate the association of the PWV with metabolic syndrome. METHODS: Among 1438 persons who visited the Internal Medicine Clinic or Health Center of a general hospital in Seoul, Korea, 384 adults (age range: 30-69 years old) were selected as study subjects. Those patients with cardiovascular disease or other systemic disease were excluded, but the patients with hypertension and diabetes mellitus were included. Ninety four (25.4%) subjects were classified as patients with metabolic syndrome when jointly applying the WHO Asia-Pacific criteria and NCEP ATPIII criteria. RESULTS: The PWV was higher in the older aged group and in the men compared to the other group. The greater the number of diagnostic criteria of the metabolic syndrome subjects had, the higher was their PWV. After adjustment for age, gender, blood pressure, BMI and fasting blood glucose, a PWV change of 1.0 m/sec increased the risk of metabolic syndrome by 1.31 times (95% CI: 0.81-2.09). The risk of metabolic syndrome was 7.62 times higher among the subjects with a PWV greater than 7.5 m/sec (95% CI: 1.07-54.42), as compared with that of subjects with a PWV less than 7.5 m/sec. CONCLUSIONS: The PWV may independently increase the risk of metabolic syndrome as a non-linear pattern. A prospective study needs to be conducted to confirm the meaning of PWV as a risk factor for metabolic syndrome, and especially to determine the cut off point.

Regulation of TFPI-2 gene expression and its role in diseases / 基础医学与临床

Tissue factor pathway inhibitor-2(TFPI-2) is a matrix-associated Kunitz-type serine proteinase inhibitor,and is considered to play an important role in some pathophysiological processes,including artherosclerosis,tumor metastasis and angiogenesis.Extracellular signals can regulate TFPI-2 gene expression through modulating promoter or signaling pathway or other factors.The mechanism,more over,has become one of the focus in recent years.

Changes and significance of inflammation factors in model rabbit with artherosclerosis induced by hyperhomocysteic acid / 吉林大学学报(医学版)

Objective To study the relationship between hyperhomocysteic acid(HHcy) and artherosclerosis.Methods 20 male rabbits were randomly divided into control and experiment groups(n=10).HHcy models were made by hypodermical injection of DL-methionine for 7 weeks.After 7 weeks homocysteic acid(Hcy),IL-1?,IL-6,and IL-8 in serum of rabbits in two groups were measured by enzyme linked immunosorbent assay(ELISA).And the pathomorphological changes of aorta were observed with HE staining.The number of NF-?B positive cells in the aorta were counted by immunohistochemical method.Results The levels of Hcy,IL-1?,IL-6 and IL-8 in experiment group were higher than those in control group(P

Macrophage Migration Inhibition Factor and Atherosclerosis and Its Related Diseases / 国际脑血管病杂志

Macrophage migration inhibition factor(MIF)is an important multifunctional cyto- kine,and it participates in many pathophysiologic processes.A growing body of evidence sug- gests that MIF plays an important role in macrophage-involved regulation of various diseases, especially in atherosclerosis,This article mainly reviews the discovery,structure,sources and biological functions of MIF,particularly the roles in artherosclerosis and its related diseases.