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Objective To screen antigen targets for immunotherapy by analyzing over-expressed genes, and to identify significant pathways and molecular mechanisms in esophageal cancer by using bioinformatic methods such as enrichment analysis, protein-protein interaction (PPI) network, and survival analysis based on the Gene Expression Omnibus (GEO) database.Methods By screening with highly expressed genes, we mainly analyzed proteins MUC13 and EPCAM with transmembrane domain and antigen epitope from TMHMM and IEDB websites. Significant genes and pathways associated with the pathogenesis of esophageal cancer were identified using enrichment analysis, PPI network, and survival analysis. Several software and platforms including Prism 8, R language, Cytoscape, DAVID, STRING, and GEPIA platform were used in the search and/or figure creation.Results Genes MUC13 and EPCAM were over-expressed with several antigen epitopes in esophageal squamous cell carcinoma (ESCC) tissue. Enrichment analysis revealed that the process of keratinization was focused and a series of genes were related with the development of esophageal cancer. Four genes including ALDH3A1, C2, SLC6A1,and ZBTB7C were screened with significant P value of survival curve.Conclusions Genes MUC13 and EPCAM may be promising antigen targets or biomarkers for esophageal cancer. Keratinization may greatly impact the pathogenesis of esophageal cancer. Genes ALDH3A1, C2, SLC6A1,and ZBTB7C may play important roles in the development of esophageal cancer.
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Humans , Esophageal Neoplasms/metabolism , Esophageal Squamous Cell Carcinoma/metabolism , Epithelial Cell Adhesion Molecule/metabolism , Gene Expression Profiling/methods , Gene Regulatory Networks , Gene Expression , Gene Expression Regulation, Neoplastic , Intracellular Signaling Peptides and ProteinsABSTRACT
In the Cox proportional hazards regression model, which is the most commonly used model in survival analysis, the effects of independent variables on survival may not be constant over time and proportionality cannot be achieved, especially when long-term follow-up is required. When this occurs, it would be better to use alternative methods that are more powerful for the evaluation of various effective independent variables, such as milestone survival analysis, restricted mean survival time analysis (RMST), area under the survival curve (AUSC) method, parametric accelerated failure time (AFT), machine learning, nomograms, and offset variable in logistic regression. The aim
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Objective To investigate the changes of urinary microalbumin(mALB)and type B urinary na-triuretic peptide(BNP)in the patients with diabetic nephropathy(DN)complicating heart failure and their in-fluence on recent prognosis.Methods A total of 73 patients with diabetes mellitus(DN)complicating heart failure from January 2014 to December 2016 were selected as the observation group and 73 persons undergoing healthy physical examination were selected as the control group.The urinary mALB and BNP levels in the two groups and the patients with different heart function grades were analyzed.The receiver operating characteris-tic(ROC)curve and survival curve were drawn for analyzing their diagnostic value and influence on progno-sis.Results The levels of urinary mALB and BNP levels in the observation group were significantly higher than those in the control group,the difference was statistically significant(P< 0.05).In the observation group,the poorer the heart function,the more obvious the urinary mALB and BNP abnormality,the difference was statistically significant(P<0.05).When the critical value of urinary mALB for diagnosing DN complica-ting heart failure was 18.56 ng/L,the area under the curve(AUC),95% CI,sensitivity and specificity were 0.983,0.964 -0.999,0.986 and 0.900 respectively,the difference was statistically significant(P< 0.05);
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Objective To study the prognosis of gastroesophageal reflux disease ( GERD) in children, and explore the factors which impacts on the prognosis of GERD. Methods One hundred and thirteen children with GERD were enrolled on the basis of positive result of 24-hour pH-monitoring between January 2007 and November 2011. The number of patients who were followed up was 87,and the parents of children were contacted with the telephone. The prognosis was evaluated by comparing the degree of patients′symptom relief,and the cumulative symptom relief rate was calculated by Kaplan-meier product limit method. The univariate Log-rank test and the COX proportional hazardmodel multivariate analysis were applied to detect the factors impacting on the prognosis,including age,gender,the regularity of treatment,reflux index,and Boix-Ochoa standard score,with esophageal hiatal hernia or without,receiving surgical treatment or not,the diet and lifestyle improved or not,receiving anti-acid treatment or not,as well as with allergies his-tory or without. Results At last,76 out of 87 children had symptom relieved. Survival curve showed the cumulative symptom relief rate at different time points,the median cumulative symptom relief rate reached 6 months,the final relief rate was close to 90. 0%,and the continuous treatment time was 44 months. The study showed that 14. 9% (13/87 ca-ses) of children′s growth and development were affected and the life and learning in 16. 1% (14/87 cases) of children were impacted. Age (P=0. 012,Wald=6. 376) and the regularity of treatment (P=0. 000,Wald=13. 059) were the risk factors in the prognosis of GERD. Conclusions Age and the treatment regularity were the factors in the prognosis. The children aged more than 1-year old have poor prognosis compared with those less than 1-year old,and the irregular treatment is the risk factor in the prognosis.
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Objective To compare the Chinese'92 and 2008 staging systems of nasopharyngeal carcinoma (NPC) based on the long term survival of the patients. Methods Clinical data of 498 NPC patients treated with definitive IMRT were retrospectively analyzed. The distributions of patients in the two staging systems were compared. The long term outcomes according to T, N and overall stages in each system were evaluated. Kappa value and Pearson coefficient were used to evaluate the agreement and correlation of the two systems. Results The distributions of both T and N stage between'92 and 2008 stage systems were different. In both staging systems, the local recurrence-free survival (LRFS) curves of T_1, T_2 andT_3 were close up (even overlaped), though they were apart from T_4. The distant metastasis-free survival (DMFS) curves overlaped of N_1 and N_2 in the'92 staging system, while separated of N_1, N_2 and N_3 in the 2008 staging system. Significant difference of DMFS was not found between N, and N_2 in'92 staging system, while did exist among N_0, N_1, N_2 and N_3 stages in 2008 staging system. In the both staging systems, the disease-specific survival (DSS) of stage Ⅰ did not significantly differ from that of stage Ⅱ or Ⅲ. The statistical analysis showed the conformality of DSS curves in the two system was 89% (Kappa =0. 833 ,P <0.01), with agood relative rate (r=0. 919,P<0. 01). Conclusions The difference between'92 and 2008 staging system is mainly in N stage. The 2008 N stage seems more reasonable compared with'92 N stage, which is able to better forecast the DMFS. There are some agreements and correlations between the two staging systems.
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PURPOSE: To investigate the clinical course of recess-resection (R & R) on the contralateral eye for recurrent intermittent exotropia after previous unilateral R & R surgery. METHODS: A retrospective analysis was conducted on patients who had unilateral R & R surgery and patients who had a second R & R surgery on the contralateral eye for recurrent intermittent exotropia with at least 12 months of postoperative follow-up. RESULTS: Our study included 490 patients with unilateral R & R and 119 patients with contralateral R & R. Recurrence was defined as an ocular misalignment of greater than 11PD during follow-up. Cumulative probabilities of surgical success rates were 42.7% and 66.4%, respectively (p=0.000). CONCLUSIONS: Exotropia reappeared in recurrent intermittent exotropia after contralateral R & R. However, when considering a long-term prognosis, the cumulative probabilities of surgical success were significantly higher in the second surgery.
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Humans , Exotropia , Eye , Follow-Up Studies , Prognosis , Recurrence , Retrospective StudiesABSTRACT
sing guidewire through temporary catheter is an alternative strategy, because of avoiding this avoids vein re-puncturation and doesn't increase catheter related complication.
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Objective To explore the radiosensitizatian of paclitaxel in human lung adenocareinoma cells. Methods A human lung adenocarcinoma cell line A973 was used in this study. The cytotoxicity of paclitaxel was investigated by using clonngenic assay to define the IC10,IC50 and IC90. The cells received either radiation(with different doses) alone or paclitaxel administrated before and after irradiation. Cell survival fractions were determined by clonogenic assay. Single hit multi-target model was used to determine survival curve parameters. Flow cytometry was performed to analyze the cell cycle distribution. Results The IC10, IC50 and IC90 of paclitaxel in A973 cells were 0.5,2.6 and 8.7 nmol/L,respectively. According to Do,Dq and SF2 value,the sensitivity enhancement ratio(SER) of IC10 was 0.97,1.01 and 2.00 when paclitaxel was added before irradiation, and 0.97,1.02 and 1.02 when after irradiation ; The SER of IC50 was 1.06,129.00 and 2.61 when paclitaxel was added before irradiation, and 0.94,220. O0 and 2.14 when after irradiation ;The SER of IC90 were 1.00,220. 00 and 2.09 when paclitaxel was added before irradiation,and 0.98,220.00 and 2.09 when after irradiation. The IC10 of paclitaxei failed to increase G2+M arrest of A973 cells.The maximal G2+M accumulation was reached at 2 h and 18 h after IC50 and IC90 of paclitaxel treatment,respectively. Conclusions Paclitaxel is able to enhance the radiation sensitivity of A973 cells. Sequence of treatment is not associated with radiosensitivity. Moderate and high dose of paclitaxel combined with low-dose radiation can produce the best effect of radiosensitiation.
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BACKGROUND: IgA nephropathy is recognized as a disease affecting primarily young men under 30 years of age but it is relatively uncommon over 50 years of age. Findings on clinical and histological presentation and outcome of over 50 years of age have rarely published in Korea. METHODS: Between Febrary 1994 and July 2003, one hundred and thirty nine IgAN patients were recruited over 8 years. Nineteen patients over age 50 were compared to one hundred and twenty patients under age 50 clinical, histological findings, 5-Yr renal survival rate. Mean post-biopsy follow-up month was 23.8 +/- 23.5 months. RESULTS: Both group of patients were similar to baseline for gross hematuria, Male and female ratio. But older patients had a higher incidence of daily for 24 hour urine protein (p=0.010), systolic and diastolic blood pressure (p=0.010, p<0.01), serum C3 (p=0.001) and serum C4 (p=0.003). Albumin (p=0.011), creatinine clearance (p<0.01) were significantly lower in the older patients at the time of renal biopsy. Histologic grade IV was more common in the older patients (p=0.001). Moderate to severe mesangial proliferation (p=0.001) and crescent formation (p=0.043), arteriolosclerosis (p=0.006) were more common in older patients. Mesangial small deposition of IgA (p=0.007) and glomerular peripheral deposition of IgG, IgA, C1q (p=0.024, p=0.014, p=0.009) were more common in older patients than in under 50 years of age. Mesangial small electron dense deposits were more common in older patients than in younger patients (p=0.031). CRF (Ccr < 60 mL/min) was confirmed 55.6% over age 50 and 27.4% under age 50 (p=0.017). Renal replacement therapy was done 15.8% over age 50 and 4.3% under age 50 (p=0.048). 5-Year renal survival curves showed that kidney survival rate was 44.4% over age 50 and 72.6% under age 50 (p=0.0248). CONCLUSION: Poor prognostic factors were more common over age 50 than under age 50. CRF and renal replacement therapy were more common over age 50. Analysis of renal survival curves shows that the probability of developing ESRF increase after age 50. But, prolonged prospective follow-up is necessary to confirm this trend.
Subject(s)
Female , Humans , Male , Arteriolosclerosis , Biopsy , Blood Pressure , Creatinine , Follow-Up Studies , Glomerulonephritis, IGA , Hematuria , Immunoglobulin A , Immunoglobulin G , Incidence , Kidney , Korea , Prognosis , Renal Replacement Therapy , Survival RateABSTRACT
PURPOSE: To determine the clinical course of recurrent exotropia after a secondary operation for exotropia. METHODS: The surgical results in 58 patients who had undergone reoperation for recurrent exotropia (reoperation group) were retrospectively investigated and compared with those of 100 patients who had undergone primary strabismus surgery only (primary operation group) using survival analysis. RESULTS: In the reoperation group, recurrence occurred in 19 of the 58 patients (33%). Survival analysis revealed that the recurrence rates in the reoperation group were significantly lower than those in the primary operation group at the same follow-up period after the corresponding strabismus surgery (p=0.018). The distant esodeviation at the postoperative 1st week after reoperation was the only significant factor associated with the recurrence after reoperation (p=0.01). CONCLUSIONS: Exotropia did recur after a secondary operation, although the recurrence rate was lower than that after a primary operation only.
Subject(s)
Child , Female , Humans , Male , Comparative Study , Exotropia/physiopathology , Postoperative Period , Recurrence , Reoperation , Retrospective Studies , Survival AnalysisABSTRACT
BACKGROUND: Paraquat is highly toxic herbicide used in agriculture, and mortality of paraquat poisoning is very high. The predictions of severity of poisoning assessed with the amount of ingestion, results of urine dithionite test and laboratory findings are not accurate. The aims of this study are to investigate whether Hart's probability of survival curves are useful for predicting outcomes of paraquat-poisoned patients and which factors influence the outcomes of the patients. METHODS: We grouped 175 patients with paraquat poisoning into seven groups using curves of probability of survival suggested by Hart, et al. Group A was patients with plasma paraquat concentration below 90% probability of survival curve, group B patients with paraquat levels between 90% and 70% probability of survival curves, group C patients with paraquat levels between 70 and 50% probability of survival curves, group D the ones with paraquat levels between 50% and 30% probability of survival curves, group E the ones with paraquat levels between 30 and 20% probability of survival curves, group F paraquat levels between 20 and 10% probability of survival curves, and group G patients with paraquat levels above 10% probability of survival curve. And we analyzed the survival and mortality rates of each groups. RESULTS: The mortality rates of groups A, B, C, D, E, F and G were 7.4%, 26.7%, 37.5%, 55.6%, 63.6 %, 83.3% and 96.8% respectively (p<0.0005). The most important risk factor for death of patients was the paraquat concentration grouping with odds ratios (95% CI) of 4.4 (0.7-27.5, group B), 7.2 (0.9-54.9, group C), 15.0 (52.1-105.6, group D), 21.0 (3.2-139.7, group E), 60.0 (7.4-487.1, group F), and 359.9 (56.9- 2277.9, group G). CONCIUSION: In assessing prognosis of patients with paraquat poisoning, grouping of the patients using initial plasma paraquat concentrations is the most important, and the policy of treatment can be decided according the results.
Subject(s)
Humans , Agriculture , Dithionite , Eating , Logistic Models , Mortality , Odds Ratio , Paraquat , Plasma , Poisoning , Prognosis , Risk FactorsABSTRACT
BACKGROUND: Paraquat is highly toxic herbicide used in agriculture, and mortality of paraquat poisoning is very high. The predictions of severity of poisoning assessed with the amount of ingestion, results of urine dithionite test and laboratory findings are not accurate. The aims of this study are to investigate whether Hart's probability of survival curves are useful for predicting outcomes of paraquat-poisoned patients and which factors influence the outcomes of the patients. METHODS: We grouped 175 patients with paraquat poisoning into seven groups using curves of probability of survival suggested by Hart, et al. Group A was patients with plasma paraquat concentration below 90% probability of survival curve, group B patients with paraquat levels between 90% and 70% probability of survival curves, group C patients with paraquat levels between 70 and 50% probability of survival curves, group D the ones with paraquat levels between 50% and 30% probability of survival curves, group E the ones with paraquat levels between 30 and 20% probability of survival curves, group F paraquat levels between 20 and 10% probability of survival curves, and group G patients with paraquat levels above 10% probability of survival curve. And we analyzed the survival and mortality rates of each groups. RESULTS: The mortality rates of groups A, B, C, D, E, F and G were 7.4%, 26.7%, 37.5%, 55.6%, 63.6 %, 83.3% and 96.8% respectively (p<0.0005). The most important risk factor for death of patients was the paraquat concentration grouping with odds ratios (95% CI) of 4.4 (0.7-27.5, group B), 7.2 (0.9-54.9, group C), 15.0 (52.1-105.6, group D), 21.0 (3.2-139.7, group E), 60.0 (7.4-487.1, group F), and 359.9 (56.9- 2277.9, group G). CONCIUSION: In assessing prognosis of patients with paraquat poisoning, grouping of the patients using initial plasma paraquat concentrations is the most important, and the policy of treatment can be decided according the results.
Subject(s)
Humans , Agriculture , Dithionite , Eating , Logistic Models , Mortality , Odds Ratio , Paraquat , Plasma , Poisoning , Prognosis , Risk FactorsABSTRACT
Most chemical carcinogens are metabolized and activated in vivo by phase I enzymes including the microsomal cytochromes P450 and epoxide hydroxylases. The carcinogens and their metabolites are detoxified by phase II enzymes that in clude various transferases such as glutathion-S-transferases (GST). Increasing numbers of studies have demonstrated the association of the polymorphisms inGSTM1 (a member of GST) andCYP1A1 genes with the susceptibility to lung cancer. Subsequently, the polymorphisms appear to be important biomarkers that provide information for assessment of exposure and total burden of environmental carcinogens. Therefore, the investigation of the polymorphisms in these genes will provide information not only for the prediction of individual cancer risk but also for the prevention of cancer. In this review, we will summarize the polymorphisms in theGSTM1 andCYP1A1 genes and their relation to lung cancer susceptibility.
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A cigarrinha-das-pastagens Deois flavopicta StÕl é a principal praga de pastagens cultivadas nos Cerrados do Brasil Central. O inseto apresenta distribuição sazonal com três gerações anuais discretas durante a estação chuvosa (setembro-abril) e população sincronizada de ovos diapáusicos durante a estação seca (maio-agosto). Experimentos em pastagens cultivadas mostraram que predadores não-específicos foram capazes de alterar significativamente as taxas de mortalidade de ovos diapáusicos e ninfas de D. flavopicta. Predação foi um fator de mortalidade independente da densidade, reduzindo os ovos diapáusicos em aproximadamente 60 por cento e ninfas de 20 por cento a 47 por cento. A taxa de mortalidade das ninfas resultante da predação não variou significativamente entre a primeira e a segunda geração anual. No entanto, a mortalidade de ninfas na terceira geração foi significativamente mais baixa, fato relacionado ao maior grau de agregação das ninfas em refúgios espaciais. Observações diretas de interações predador-presa nas pastagens sugerem que, potencialmente, as formigas representam o grupo que mais contribui para a mortalidade observada, embora esse impacto deva ainda ser verificado experimentalmente. Taxas de mortalidade mais altas em ovos e ninfas de D. flavopicta expostos aos predadores indicam que a predação pode ser um fator importante na determinação da população de adultos. Desta forma, práticas de manejo das pastagens que perturbam a comunidade de predadores podem resultar em densidades mais altas das cigarrinhas.
The spittlebug, Deois flavopicta StÕl, is the main pest in cultivated pastures of the "Cerrados" (savanna) in the central region of Brazil. The insect has three discrete generations during the rainy season (September-April) and a synchronized population of diapausing eggs during the dry season (May-August). Experiments in cultivated pastures showed that nonspecific predators were able to affect significantly the mortality rates of diapausing eggs and nymphs of D. flavopicta. Predation was a density independent mortality factor that reduced diapausing eggs by approximately 60 percent and nymphs by 20 percent to 47 percent. The nymph mortality rate due to predation did not differ during the first and the second generations of the year. For the third generation however, the mortality rate was lower, related to a greater degree of nymphal aggregation in spatial refuges. Our direct observations indicate that among the types of predators in pastures, ants potentially make the greatest contribution to mortality, although their impact needs experimental verification. High rates of mortality of eggs and nymphs of D. flavopicta exposed to predators indicate that predation can be an important factor determining the size of adult populations. Therefore, management practices that disturb the predator community may increase the population densities of the spittlebug.
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Most chemical carcinogens are metabolized and activated in vivo by phase I enzymes including the microsomal cytochromes P450 and epoxide hydroxylases. The carcinogens and their metabolites are detoxified by phase II enzymes that include various transferases such as glutathion-S-transferases (GST). Increasing numbers of studies have demonstrated the association of the polymorphisms in GSTM1 (a member of GST) and CYP1A1 genes with the susceptibility to lung cancer. Subsequently, the polymorphisms appear to be important biomarkers that provide information for assessment of exposure and total burden of environmental carcinogens. Therefore, the investigation of the polymorphisms in these genes will provide information not only for the prediction of individual cancer risk but also for the prevention of cancer. In this review, we will summarize the polymorphisms in the GSTM1 and CYP1A1 genes and their relation to lung cancer susceptibility.
Subject(s)
Cytochrome P-450 CYP1A1ABSTRACT
BACKGROUND: The future health of our increasing senior population depends upon the interrelationship between the onset time of the first major disease, infirmity or disability and the time of death. Reduction of morbidity requires compressing the average period between two points and reducing the average level of morbidity during this period. In this article, authors demonstrated the change of mortality curve and estimated the mortality curve upto 2010. And we intend to use this result as basic data in the future for preventive health service, health promotion, and health policy for senior people. METHODS: In this study, the authors demonstrated the survival curve from 1970 to 1995. And we intended to estimate death rate according to age and year upto 2010 and construct a new forecasting model. By using this model, we can calculate the data upto 2010 and suggest mortality curve. RESULTS: According to the results, the relative and absolute rectangularization and the convergency of survival curves were observed, and all the Keyfitz's H, NH, SD and CV decreased while the life expectancy in creased in the period of 1970 to 2010. So we conclude that the hypothesis of mortality compression suggested by Fries explains the changing pattern of aged population in Korea very well. CONCLUSION: According to Fries theory, this study shows compression of mortality is exist in Korea But when we observe the tail of mortality curve, there are no evidence that life expectancy reaches to limitation in Korea and we expect life expectancy of Korean will continuously increase.
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Forecasting , Health Policy , Health Promotion , Korea , Life Expectancy , Mortality , Preventive Health ServicesABSTRACT
BACKGROUND: In this article, authors determine whether rectangularization of survival curve occurred in Korea in the period 1966-1995. Rectangularization is defined as a trend toward a more rectangular shape of the survival curve due to increased survival and concentration of deaths around the mean age of death. Authors distinguish between absolute and relative rectangularization, depending on whether an increase in life expectancy is accompanied by concentration of deaths into a smaller age interval or into a smaller proportion of total life expectancy. METHODS: In this study, the authors intended to demonstrate the survival curve according to the year 1966 and 1970 to 1995. For these purpose, the authors used the annual mortality data published by the National Statistical Office from 1966 to 1995 in Korea. Authors used measures of variability based on Keyfitz' H and the standard deviation, both life table-based. Our results show that absolute and relative rectangularization of the entire survival curve occured except mortality data of 1966 which has little reliability over the complete period. RESULTS: In this study, authors observed relative and absolute rectangularization and the survival curves of 1970 to 1995 shows the convergence between mid-60s and mid-80s. All the Keyfitz' H, NH, SD and CV decreased while the life expectancy increased in the period of 1970 to 1995. This result is similar to the mortality curve suggested by Fries according to his compression of morbidity hypothesis. CONCLUSIONS: According to Keyfitz theory, rectangularization happens to survival curve in Korea. The implications of the recent rectangularization at older ages for achieving compression of morbidity have to be exactly understood. So, the authors suggest that health policy and research must be directed to the preventive health service and health promotion to reduce morbidity, postpone infirmity and to warrant the quality of life for senior people.
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Health Policy , Health Promotion , Korea , Life Expectancy , Mortality , Preventive Health Services , Quality of LifeABSTRACT
As the population of senior people has been increased rapidly during the past 30 years in Koera, the health policy and related research in this field are strongly demanded to manage various problems which can be derived from the gerontic phenomenon. We estimated the death rates up to 2010 using the annual mortality data published by the National Statistical Office from 1970 to 1995 by time series analysis and calculated the survival curves, life expectancy by life table method with modified Graville's formular and proposed several measures which can be used in describing the theory of retangularization of survival curves and compression of mortality hypothesis. According to the results, the relative and absolute rectangularization and the convergency of survival curves were observed, and all the Keyfitz's H, NH, SD, and CV decreased while the life expectancy increased in the period of 1970 to 2010. So we conclude that the hypothesis of mortality compression suggested by Fries explains the changing pattern of aged population in Korea very well.
Subject(s)
Health Policy , Korea , Life Expectancy , Life Tables , MortalityABSTRACT
Since discovery of X-rays, radiotherapy has evolved into one of the most scientific branches of medicine and has established its role as the primary line or the secondary line of attack, after surgery,. in the treatment of malignant cancers. Nowadays its importance is illustrated by the fact that as many as 70 per cent of all pastients with cancer will receive radiation therapy at sometime during their disease process. Biologic effects-of X-rays began to be apparant soon after the discovery by Roentgen in 1895. In clinical radiotherapy, the biologic endpoint of most importance is loss of cellular reproductive ability or clonogenicity. One of the commonest ;nays to assess cell survival is to use an in vitro plating assay. We analyzed radiation effect on colony formation of HaLa. S3(SC) cell line and obtained results are as follows The plating efficiency is 0.464. The shape of cell survival curve is similar to multi-target plus single hit component model. Estimated values of Do, Dq, and extrapolation number are 150 cGy, 80 cGy and 1.7 respectively. We reported these experimental data with review of literature.