ABSTRACT
Aim: To observe the protective effects of total saponins of tribulus (TST) on cerebral ischemia reperfusion injury in rats. Methods: Rat cerebral ischemia-reperfusion injury was induced by middle cerebral artery occlusion . The neurological outcome was evaluated. CK, LDH and NO activity in serum, SOD and MDA levels of cerebral tissue were measured. Results: The levels of CK, LDH, NO, SOD and MDA in TST(30 mg·kg-1, 10 mg·kg -1) groups were significantly lower than those in model group. The level of cellular and intercellular edema was reduced markly in the pathomorphology examination. The neurological deficits was decreased by TST. Conclusion: TST has protective effects on cerebral ischemia reperfusion injury in rats.
ABSTRACT
Objective To observe the effects of total saponins of tribulus of various consistency on the differentiation of neural hippocampus stem cells. Methods The hippocampus stem cells from newborn rat were obtained by serum-free medium and single-cell cloning technique. Hippocampus stem cells were induced by total saponins of tribulus of various consistency. Nestin, BrdU, neurofilament (NF-200), glial fibrillary acidic protein (GFAP) expression were detected using immunocytochemical stains. The numbers of NF and GFAP positive cells were counted. The percentages of positive cells were obtained. Results The dissociated cells from the organ of hippocampus developed into much of indiffrentiated otopheres of nestin positive cells. The hippocampus stem cell differentiated into neurons and astrocytes with the total saponins of tribulus. The percentage of NF positive neuron in the 20 mg/kg consistency of total saponins of tribulus groups was significantly higher than that in the control group and other consistency of total saponins of tribulus groups. Conclusion Total saponins of tribulus may enhance neural hippocampus stem cells differentiate into NF positive neuron.
ABSTRACT
Aim To observe the protective effects of total saponi ns of tribulus (TST) on cerebral ischemia reperfusion injury in rats. Meth ods Rat cerebral ischemia-reperfusion injury was induced by middle cer ebral artery occlusion .The neurological outcome was evaluated. CK, LDH and NO a ctivity in serum,SOD and MDA levels of cerebral tissue were measured. Re sults The levels of CK,LDH,NO,SOD and MDA in TST(30 mg?kg -1,1 0 mg?kg -1) groups were significantly lower than those in model group. The level of cellular and intercellular edema was reduced markly in the pathomorpho logy examination. The neurological deficits was decreased by TST.Conclus ion TST has protective effects on cerebral ischemia reperfusion injur y in rats.