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Objective To explore the clinical significance and antimicrobial resistance of group B Streptococcus(GBS)isolated from midstream urine culture,aiming to provide a basis for the diagnosis and treatment of clinical urinary tract infection(UTI).Methods Information about GBS strains isolated from midstream urine culture of in-patients and outpatient in a hospital in Nanjing from February 2020 to December 2022 were retrieved through labora-tory information system,strains with complete data were screened out.Case data,urine routine,and antimicrobial susceptibility testing results were reviewed.Results A total of 9 081 non-repetitive bacterial strains were detected from midstream urine culture,including 425 GBS strains,accounting for 4.7％,ranking sixth.Strains with incom-plete data were excluded,a total of 365 patients were included in the study.169(46.3％)were males and 196(53.7％)were females,with an average age of(55.4±15.2)years.365 patients who were detected GBS were from 17 de-partments,with the highest proportion being department of urology(n=237,64.9％).The underlying diseases of patients mainly included hypertension(n=136),diabetes(n=95),urolithiasis(n=120)and urinary tumors(n=98).211 patients underwent urological surgery,all were treated with antimicrobial agents before surgery,and 205 patients underwent indwelling urinary catheters after surgery;9 patients were detected GBS from urine during the middle and advanced stage of pregnancy.36.4％(n=133),38.9％(n=142)and 24.7％(n=90)patients had GBS colony count ≤104 CFU/mL,104-105 CFU/mL,and ≥105 CFU/mL,respectively.Patients with symptoms of UTI accounted for 24.9％(n=91),and asymptomatic bacteriuria accounted for 75.1％(n=274).The incidence of UTI symptoms in males was lower than that in females(19.5％vs 29.6％,P＜0.05).As the GBS colony count in urine culture increased,the proportion of patients with symptoms of UTI showed an upward trend(P＜0.05).On the day of urine culture,the positive rates of urine routine white blood cells,leukocyte esterase,and nitrite were 53.2％,50.1％,and 3.8％,respectively.The positive rates of urine occult blood,leukocyte esterase,white blood cells,and urine protein in patients with symptomatic UTI were all higher than those with asymptomatic bacteriuria patients(all P＜0.05).No GBS were found to be resistant to penicillin,ampicillin,vancomycin,linezolid,and tigecycline.The resistance rate to levofloxacin and moxifloxacin was about 40％,and resistance rate to tetracycline and clindamycin was over 60％.Conclusion GBS isolated from urine is more common in non-pregnant adults,and only a small percentage have symptoms of UTI.The results of urine culture and urine routine should be comprehen-sively judged based on patient's clinical symptoms and signs.GBS in urine is susceptible to multiple antimicrobial agents,and clinical medication should be adopted rationally based on antimicrobial susceptibility testing result.
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Background: Dengue fever is an important tropical disease which is endemic in around 110 countries. It infects 50-100 million people worldwide per year. In India case fatality rate is 1-5% for severe Dengue. Between 2015-2017, 790 deaths have been recorded according to NVBDCP data. Global burden of Dengue has increased at least fourfold over last three decades and now 2.5 billion people at risk of disease. This study aims at determining sterile pyuria as a manifestation in patients presenting with Dengue fever, as patients may present with similar symptoms as that of urinary tract infection, thereby preventing unnecessary use of antibiotics.Methods: It is a Cross sectional observational study conducted on 100 consecutive patients with serologically proven Dengue fever. Patients satisfying inclusion and exclusion criteria underwent relevant investigations and in patients with urine routine showing pyuria, urine culture and sensitivity was done to rule out urinary tract infection and look for sterile pyuria.Results: Among 100 patients of dengue studied, age distribution being 18years to 70years, mean age was 33.27±13.2 years of them 78 were male and 22 were female. 41% patients showed pyuria in urine. 25 % patients were culture positive most common being E. coli and 16% patients had sterile pyuria.Conclusions: Sterile pyuria is not a well-recognized entity in Dengue fever and is often missed. This study shows that sterile pyuria is quite common manifestation in dengue fever which resembles urinary tract infection and therefore does not require any empirical antibiotic treatment.
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Rhabditis axei is a free-living nematode, which can occasionally invade into humans through drinking and contacting wastewater. It is usually parasitic in the digestive and urinary systems, causing the disease of rhabditelliasis axei. This paper reports a case of R. axei infection found in the urine routine examination.
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Rhabditis axei is a free-living nematode, which can occasionally invade into humans through drinking and contacting wastewater. It is usually parasitic in the digestive and urinary systems, causing the disease of rhabditelliasis axei. This paper reports a case of R. axei infection found in the urine routine examination.
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Objective To observe the effect of the application of Diri urine test workstation(consisting of FUS-200 urine analyzer and H-800 automatic urine analyzer) and microscope in routine urine test. Methods 378 cases of urine samples of patients in Longquan municipal people's hospital were selected randomLy. Urine routine test were carried out by three methods. The main test items were white blood cells (WBC) and red blood cell (RBC), the test results were compared. Results H-800 automatic urine analyzer: the sensitivity of RBC was 94.3%, the specificity was 93.5%, the sensitivity of WBC was 85.4%, the specificity was 99.4%; FUS-200 urine analyzer: the sensitivity of RBC was 85.7%, the specificity was 98.4%, the sensitivity of WBC was 92.7%, the specificity was 99.4%. Conclusion In order to get the most accurate results, we need to make a comprehensive analysis of the three kinds of test methods in order to provide a reliable basis for clinical diagnosis and treatment.
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Objective To observe the effect of the application of Diri urine test workstation(consisting of FUS-200 urine analyzer and H-800 automatic urine analyzer) and microscope in routine urine test. Methods 378 cases of urine samples of patients in Longquan municipal people's hospital were selected randomLy. Urine routine test were carried out by three methods. The main test items were white blood cells (WBC) and red blood cell (RBC), the test results were compared. Results H-800 automatic urine analyzer: the sensitivity of RBC was 94.3%, the specificity was 93.5%, the sensitivity of WBC was 85.4%, the specificity was 99.4%; FUS-200 urine analyzer: the sensitivity of RBC was 85.7%, the specificity was 98.4%, the sensitivity of WBC was 92.7%, the specificity was 99.4%. Conclusion In order to get the most accurate results, we need to make a comprehensive analysis of the three kinds of test methods in order to provide a reliable basis for clinical diagnosis and treatment.
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Objective To analyze the factors related to the disqualification of urine routine and stool routine, and to formulate corresponding solutions. Methods In April 2014 to April 2017 our collected 600 cases of hospitalized patients, urine and stool samples for routine detection, made clear that cause unqualified samples related factors, and develop effective solution strategy. Results 600 cases in the selected routine urine, excrement and urine routine test specimens, the unqualified samples of total 110 cases, fraction defective is 18.33%, 490 qualified samples, percent of pass is 81.67%, the difference was statistically significant (P<0.05).There are a number of factors that cause the sample to fail, such as the contamination of the sample, the length of storage, the operation and the preparation. Conclusion In the process of actual diagnosis of disease, clinical result in urine, feces routine test specimen unqualified often appear, the reason is to a great extent, and the related nursing staff in urine and stool samples of patients before the lack of relevant guidance and so on, and nursing staff after the complete collection of specimens does not usually in accordance with the relevant standard of serious check, to avoid the sample inspection is unqualified, hospitals need to develop scientific inspection process, nurses in practice need to strict adherence to the process, and through intensive training, promote nursing staff awareness test, can to improve the qualification rate of sample test provide strong guarantee.
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Objective To study the relationship between urine routine and urinary sediment in urine test.Methods 80 cases of patients accepted urine test in our hospital from May 2014 to May 2016 were selected, these patients were divided into urine routine group (n=40) and urinary sediment group (n=40) two groups according to the detection methods, the sampling collected time, obtained time, Resultstime, urine protein, white blood cells, red blood cells, crystallization, tube type test results of the two groups were statistically analyzed.ResultsThe differences of sampling collected time, obtained time, results time between the two groups were not significant;the detection positive rates of urinary protein, white blood cells, red blood cells, crystallization, tube type of the urine routine group were 15%, 20%, 22.5%, 27.5%, 25%, respectively, the urine sediment group were 15%, 20%, 25%, 30%, 27.5%, respectively.The differences of detection positive rates of urinary protein, white blood cells, red blood cells, crystallization, tube type between the two groups were not significant;the detection positive coincidence rates of urinary protein, white blood cells, red blood cells, crystallization, tube type of the two groups were 15%, 17.5%, 22.5%, 25% 22.5%, respectively, the negative coincidence rates were 82.5%, 77.5%, 72.5%, 72.5%, 70%, respectively, the total detection rates were 97.5%, 95%, 95%, 97.5%, 92.5%, respectively.Conclusion Combined detection of urine routine and urinary sediment in urine test can significantly improve detection accuracy.