Effects of aerobic exercise on myocardial injury, NF-B expression, glucolipid metabolism and inflammatory factors in rats with coronary heart disease

Abstract Objective To investigate the influence of aerobic exercise on myocardial injury, NF-B expression, glucolipid metabolism and inflammatory factors in rats with Coronary Heart Disease (CHD) and explore the possible causative role. Methods 45 Sprague Dawley® rats were randomized into model, control and experimental groups. A high-fat diet was adopted for generating a rat CHD model, and the experimental group was given a 4-week aerobic exercise intervention. ECG was utilized to evaluate the cardiac function of the rats; HE staining to evaluate the damage of myocardial tissue; TUNEL staining to evaluate cardiomyocyte apoptosis level; ELISA to assay the contents of inflammatory factors and glucolipid metabolism in cardiomyocytes; qPCR to assay IB- and NF-B mRNA expression; Western-blot to assay the apoptosis-related proteins and NF-B signaling pathway-related proteins expressions in myocardial tissue. Results In contrast to the model group, aerobic exercise strongly improved the rat's cardiac function and glucolipid metabolism (p < 0.01), enhanced IL-10 content, Bcl-2/Bax level as well as IB- protein and mRNA expression (p < 0.01), and reduced myocardial injury and cardiomyocyte apoptosis, the contents of IL-6, IL-1 and TNF-, Caspase 3 level, NF-B mRNA and protein expression and p-p38 and p-STAT3 expressions (p < 0.01). Conclusion Aerobic exercise can not only effectively reduce myocardial injury, the release of inflammatory factors and NF-B expression in CHD rats, but also improve cardiac function and glucolipid metabolism. Its mechanism is likely to be related to the inhibition of the NF-B signaling pathway.

Anti-inflammatory effects of salidroside on lps-induced bv2 microglia cells via pi3k/akt signaling pathway / 中国药理学通报

 Aim To investigate the anti-inflammatory effect of salidroside on lipopolysaccharide (LPS)-induced BV2 microglia and its mechanism. Methods The model of LPS-induced injury in BV2 microglia was established. The expression of cytokines IL-6, IL-1β and TNF-α mRNA was detected by qPCR, and the expression of Akt, p-Akt, nuclear protein NF-κB p50 protein was dectected by Western blot with different concentrations of salidroside treatment. The model was sequentially treated with PI3K inhibitor LY294002 for 30 min, and after the treatment of salidroside, the expressions of Akt, p-Akt, nuclear protein NF-κB p50, IL-6, IL-1β, TNF-α and other indicators were dectected by Western blot. Results Compared with model, salidroside reduced the expression of IL-6, IL-1β and TNF-α mRNA in BV2 microglia, improved the expression of p-Akt protein, and reduced the nuclear protein NF-κB p50. After the treatment of LY294002, salidroside had no obvious effect on the expression of p-Akt, nuclear protein NF-κB p50, IL-6, IL-1β. Conclusions Salidroside can inhibit LPS-induced BV2 microglia inflammatory reaction by activating PI3K/Akt signaling pathway, promoting Akt phosphorylation, inhibiting NF-κB p50 nuclear transcription, and inhibiting cytokines.

Regulation of il-1 and TNF-α on metabolic enzyme cyp2e1 in immunological liver injury in rats / 中国药理学通报

Aim To investigate the regulation and mechanism of inflammatory cytokines interleukin 1β (IL-1β) and tumor necrosis factor α (TNF-α) on the drug metabolism enzyme cytochrome P450 2E1 (CYP2E1) during immune-mediated liver injury. Methods By injection of Bacillus Calmette-Guerin(BCG, 125 mg·kg-1) prepared rat model of immunological liver damage. To detection of CYP2E1 probe drug chlorzoxazone in blood concentration changes of rats over time reflects its metabolic activity by high-performance liquid chromatography (HPLC) assay. The changes of IL-1β and TNF-α in liver tissue of rats were detected by ELISA. The expression of CYP2E1 protein was determined by Western blot analysis. Results After 14 days of BCG injection of rats, a large number of mononuclear cells and lymphocytes infiltrated around the liver parenchyma and the portal area, resulting in a large number of granuloma masses with different sizes and diffuse distribution, IL-1β and TNF-α high expression (P < 0.01), CYP2E1 metabolic activity and protein expression were significantly decreased (P < 0.05). The protein content of CYP2E1 was significantly negatively correlated with IL-1β (r=0.222, P=0.027). Activation of PDTC-mediated nuclear factor-kappa B (NF-κB) inhibited the high expression of IL-1β and TNF-α in liver tissue and slowed down the down-regulation of CYP2E1 metabolism and protein expression. Conclusions NF-κB may be involved in the regulation of CYP2E1 by regulating the inflammatory cytokines IL-1β and TNF-α.

A surface antigen of Orientia tsutsugamushi activates human monocyte-derived dendritic cells via nuclear factor-kB & p38 mitogen-activated protein kinase pathways

Background & objectives: Scrub typhus is a chigger-borne disease caused by Orientia tsutsugamushi. The immunological reactions to O. tsutsugamushi infection are not completely understood. In this study, we investigated the response of dendritic cells (DCs) to a major 56-kDa scrub typhus antigen Sta56. Methods: Monocyte-derived human DCs were incubated with different concentrations of recombinant Sta56 and analyzed for maturation based on phagocytic capacity, the ability to induce T-cell proliferation, expression of surface markers, cytokine secretion and activation of toll-like receptor (TLR)-dependent signalling pathways. Results: Treatment of DCs with Sta56 induced cell surface expression of CD80, CD83, CD86 and MHC Class II increased the production of interleukin-12 (IL-12) p40, IL-12 p70 and IL-10 and decreased DC phagocytic capacity. Furthermore, Sta56 increased the ability of DCs to activate T-cell proliferation and interferon-? secretion. TLR4-specific antibodies neutralized Sta56-elicited effects on DC maturation, suggesting direct interaction between Sta56 and TLR4. Moreover, Sta56 activated nuclear factor (NF)-?B and p38 mitogen-activated protein kinase (MAPK) signalling as evidenced by decrease in Sta56-induced cytokine production and surface marker expression by specific inhibitors helenalin and SB203580, respectively, and increase in I?B? and p38 phosphorylation and NF-?B-DNA binding. Interpretation & conclusions: Our results showed that the surface antigen of O. tsutsugamushi activated DCs through interaction with TLR4 and activation of MAPK and NF-?B signalling, suggesting Sta56 as a potential candidate molecule for the development of vaccine against scrub typhus.

Nephroprotective Effects of Vernonia amygdalina in Alloxan-induced Diabetes in Rats

Background: Diabetic nephropathy is a leading cause of end-stage renal failure worldwide. Purpose: The methanol leaf extract of Vernonia amygdalina (MLVA) was thus investigated for its nephroprotective effects in diabetes.Materials and Methods: Diabetes was induced in male Wistar rats by a single intraperitoneal (I.P) injection of a freshly prepared solution of Alloxan monohydrate (100 mg/kg). Forty-eight hours after alloxan administration, rats with fasting blood glucose levels of 200 mg/dl and above were used for the study. Animals were grouped into five (A-E) of nine animals each. Group A was non-diabetic non treated control; Group B animals were the diabetic untreated control rats while groups C, D and E animals were diabetic and treated with glibenclamide, MLVA 200 mg/kg and MVLA 400 mg/kg respectively. Biochemical changes were evaluated by measuring the serum markers of kidney damage (creatinine and blood urea nitrogen). Markers of oxidative stress and antioxidant activities were measured in renal tissues. Histopathological and immunohistochemical changes were also evaluated.Results: Four-week administration of MLVA produced significant (p<0.05) decrease in serum creatinine, urea, and oxidative markers but it caused a significant increase in enzymic and nonenzymic antioxidant as well as downregulation of Nuclear Factor kappa-light-chain-enhancer of activated B cells (NF-?B) and upregulation of B-cell lymphoma 2 (Bcl-2).Conclusion: MLVA ameliorates diabetic nephropathy through its antioxidant, anti-inflammatory and antiapoptotic effects

Influence of the dopaminergic system, CREB, and transcription factor-B on cocaine neurotoxicity

Cocaine is a widely used drug and its abuse is associated with physical, psychiatric and social problems. Abnormalities in newborns have been demonstrated to be due to the toxic effects of cocaine during fetal development. The mechanism by which cocaine causes neurological damage is complex and involves interactions of the drug with several neurotransmitter systems, such as the increase of extracellular levels of dopamine and free radicals, and modulation of transcription factors. The aim of this review was to evaluate the importance of the dopaminergic system and the participation of inflammatory signaling in cocaine neurotoxicity. Our study showed that cocaine activates the transcription factors NF-κB and CREB, which regulate genes involved in cellular death. GBR 12909 (an inhibitor of dopamine reuptake), lidocaine (a local anesthetic), and dopamine did not activate NF-κB in the same way as cocaine. However, the attenuation of NF-κB activity after the pretreatment of the cells with SCH 23390, a D1 receptor antagonist, suggests that the activation of NF-κB by cocaine is, at least partially, due to activation of D1 receptors. NF-κB seems to have a protective role in these cells because its inhibition increased cellular death caused by cocaine. The increase in BDNF (brain-derived neurotrophic factor) mRNA can also be related to the protective role of both CREB and NF-κB transcription factors. An understanding of the mechanisms by which cocaine induces cell death in the brain will contribute to the development of new therapies for drug abusers, which can help to slow down the progress of degenerative processes.

Efeito da suplementação com L-glutamina livre e na forma de dipeptídeo sobre eixo glutamina-glutationa, sistema imune, sistema inflamatório e vias de sinalização proteica em camundongos submetidos à endotoxemia / Effects of dietary supplementation with L-glutamine in the free and dipeptide forms on glutamine-glutathione axis, immune system, inflammatory system and protein signaling pathwaysin mice submitted to endotoxemia

A sepse é a principal causa de morte em unidades de terapia intensiva (UTIs) no mundo. A reduzida disponibilidade do aminoácido mais abundante do organismo, a glutamina contribui para o complicado estado catabólico da sepse. No presente estudo investigamos os efeitos da suplementação oral com L-glutamina e L-alanina (GLN+ALA), ambos na norma livre e como dipeptídeo, L-alanil-L-glutamina (DIP), sobre o eixo glutamina-glutationa (GSH), sistema imune, inflamação, proteínas de choque térmico (HSPs) e expressão de genes envolvidos com vias de sinalização proteica em animais endotoxêmicos. Camundongos C57/B6 foram submetidos à endotoxemia (Escherichia coli LPS, 5 mg.kg-1, grupo LPS) e suplementados por 48 horas com L-glutamina (1 g.kg-1) e L-alanina (0,61 g.kg-1, grupo GLN+ALA-LPS) ou 1,49 g.kg-1 de DIP (grupo DIP-LPS). A endotoxemia promoveu depleção da concentração de glutamina no plasma (71%), músculo esquelético (50%) e fígado (49%), quando comparado ao grupo CTRL, sendo restauradas nos grupos DIP-LPS e GLN+ALA-LPS (P<0,05), fato que atenuou a redução da GSH e o estado redox (taxa GSSG/GSH) em eritrócitos circulantes, musculo e fígado (P<0,05). A suplementação em animais endotoxêmicos resultou em uma upregulation dos genes GSR, GPX1 e GCLC no músculo e fígado. A concentração das citocinas plasmáticasTNF-α, IL-6, IL-1ß e IL-10 foi atenuada pelas suplementações, bem como a expressão de mRNAs envolvidos com a resposta inflamatória, ativadas pela via do NF-κB(P<0,05). Concomitantemente, verificou-se aumento da capacidade proliferativa de linfócitos T e B circulantes nos grupos GLN+ALA-LPS e DIP-LPS. A expressão de mRNAs e a concentração de HSPs no tecido muscular foi restabelecida pelas suplementações, contudo, a expressão mRNAs relacionados às vias de síntese e degradação proteica foi somente estimulada no tecido hepático(P<0,05). Os resultados do presente estudo demonstram que a suplementação por via oral com GLN+ALA ou DIP podem ser utilizados clinicamente como métodos nutricionais em reverter o quadro de depressão da disponibilidade de glutamina corporal da sepse induzida por LPS, tendo impacto no eixo glutamina-glutationa, sistema imune e inflamatório

Sepsis is the leading cause of death inintensive care units (ICUs) in the world.The availability ofthe most abundant amino acid in the body, glutamine, is reduced in this situation, fact that contribute to the complicated catabolic state of sepsis. In the present study, we investigated the effects of oral supplementation with L-glutamine and L-alanine (GLN+ALA), both in their free form and as a dipeptide, L-alanyl-L-glutamine (DIP) on glutamine-glutathione axis (GSH), immune and inflammatory system, heat shock proteins (HSPs) expression and gene expressions involved in protein signaling pathways during endotoxemia. C57/B6 mice were subjected to endotoxemia (Escherichia coli LPS, 5 mg.kg-1, LPS group) and supplemented for 48 hours with L-glutamine (1 g.kg-1) plus L-alanine(0.61 g.kg-1, GLN+ALA-LPS group) or 1.49 g.kg-1of DIP (DIP-LPS group). Endotoxemia promoted depletion glutamine concentration in plasma (71%), skeletal muscle (50%) and liver (49%), when compared to the CTRL group, and was restored in the DIP-LPS e GLN+ALA-LPS (P<0.05), fact that attenuate the reduction of GSH and the redox state (GSSG/GSH rate) in circulating erythrocytes, liver and muscle (P<0.05). Supplementations in endotoxemic mice resulted in upregulation of GSR, GCLC and GPX1 genes in muscle and liver. Plasma concentration of TNF-α, IL-6, IL-1ß and IL-10 were attenuated by supplementation as well as the expression of mRNAs involved in the inflammatory response, activated by NFκ-B pathway (P <0.05). At the same time, high proliferative capacity of circulating T and B lymphocytes GLN+ALA-LPS e DIP-LPS were observed. HSPs (protein and mRNAs) and in muscle were restored by the supplements, however, the mRNAs expression related to the synthesis and degradation of protein pathways was only stimulated in the liver (P <0.05). Our results demonstrate that oral supplementation with GLN+ALA or DIP can be used as clinically nutritional methods to reverse the depression of body glutamine availability during sepsis induced by LPS, impacting on the glutamine-glutathione axis, immune and inflammatory system

Effect of NF-kB antisense oligonucleotides on tumorigenesis and angiogenesis of gastric cancer cells in nude mice / 肿瘤

Objective: To investigate the effects of transfection of antisense oligodeoxynucleotide (ASODN) of nuclear transcription factor κB (NF-κB) on tumorigenesis and angiogenesis of human gastric cancer cell line SGC7901 in nude mice. Methods: ASODN targeted P65 promoter region of NF-κB was designed, synthesized, and transfected into gastric cancer cell line SGC-7901. Fluorescent staining was used to observe transfection efficacy. The growth-inhibiting rate of cells was measured by MTT assay in vitro. The subcutaneous xenograft model of human gastric cancer cells was established in nude mice and the tumor growth curve was observed. The microvessel density (MVD) was detected by HE staining, and the apoptotic index (AI) was quantitatively determined by terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate fluorescence nick end labeling (TUNEL). Results: The cell proliferation was significantly inhibited in P65 ASODN-transfected group in vitro (P < 0.05). In vivo tumor formation test showed that the tumor volume in nude mice in ASODN group was obviously smaller than that of other groups (P < 0.05) and the AI was significantly higher than that of other groups (P < 0.001). The MVD in ASODN group was markedly lower than that of other groups (P < 0.01). Conclusion: Transfection of P65 ASODN significantly inhibited the proliferation of SGC-7901 cells in vitro. The mechanism may be related to induction of cell apoptosis and inhibition of angiogenesis caused by P65 ASODN.

Papel inmunológico de la progesterona en el mantenimiento del embarazo / Immunological role of progesterone in the maintenance of pregnancy

Progesterone is an essential hormone for pregnancy maintenance. This hormone acts by binding to its intracellular receptor or by rapid non-genomic actions to regulate a wide variety of biological functions in the feto-placental unit. Progesterone regulates blastocyst implantation and placental development by inducing immunosupression through type Th2 cytokines secretion. This review summarizes current research about the role of progesterone as critical regulator of expression and secretion of cytokines by T-cell and other placental cells.

La progesterona es una hormona esteroide muy versátil y esencial para el mantenimiento del embarazo. El principal mecanismo de acción de la progesterona es el clásico, vía receptor intracelular, regulando diversas funciones, aspectos celulares y vías moleculares implicadas en el proceso de la implantación. Asimismo existen mecanismos adicionales que no dependen de la interacción del complejo hormona receptor con la maquinaria transcripcional y que son capaces de regular rápidamente cascadas de señalización que determinarán la respuesta de la célula. En particular se ha demostrado que la progesterona ejerce efectos inmunosupresores durante la gestación al favorecer la secreción de citocinas de tipo Th2 por los linfocitos T, evento importante para regular el sistema inmunológico materno y evitar el rechazo de la placenta. El objetivo de esta revisión se centra en analizar la influencia de la progesterona en la interfase materno-fetal sobre la expresión y secreción de citocinas por las células T y no T como es el caso del trofoblasto.

The effects of taurine on the expression of Caspase-3 and NF-?B in dorsal root ganglion diabetic rats / 重庆医科大学学报

16.7mmol/L)48hours after injection.The successful diabetic rats models were randomly divided into diabetic mellitus(DM) group and taurine(TA) group.DM and TA groups were subdivided into DM1,DM2 and TA1,TA2 groups according to the duration of diabetes.The expression of Caspase-3 and NF-?B mRNA were studied by reverse-transcriptase polymerase chain reaction(RT-PCR) method.Results:NO obvious expression of Caspase-3 and NF-?B mRNA were found in the nomal groups compared with DM group after the 4th and 8th week.Under the intervention of taurine,the expression of Caspase-3 and NF-?B mRNA were inhibited compared with those in model.group(P

Expression and clinicopathological significance of NF? B p65 and PTEN in laryngeal squamous cell carcinoma / 重庆医科大学学报

Objective:To investigate the expression of NF?B p65 and PTEN in laryngeal squamous cell carcinoma(LSCC) and their clinical significance.Methods:Immunohistochemical staining was used to examine the expression of NF?B p65 and PTEN in 52 cases of LSCC and 30 cases of adjacent normal tissues.Results:The expression positive rates of NF?B p65 in LSCC were obviously higher than those in adjacent normal tissues(P0.05).The Spearman analysis indicated that the expression level of NF?B p65 was negatively correlated with that of PTEN significantly(rs=-0.580,P

Role of Nuclear Factor-kappa B Activation in Inflammatory Effect Induced by PM2.5 from Beijing Urban Area in A549 Cells / 环境与健康杂志

Objective To study the adverse effects of PM2.5 from Beijing urban areas on human lung adenocarcinoma cells(A549) and the expression of NF-?B in the cells. Methods A549 cells were treated with PM2.5 at 25,50,100 and 200 ?g/ml for 24 h. Cytotoxicity of PM2.5 was measured by MTT assay. The activity of NF-?B was measured by ELISA assay. Western blot method was used to detect the expression of NF-?B and NO levels was determined by using the nitrate reductase method. Results PM2.5 could induce A549 cell proliferation at low doses,but inhibit cell proliferation at high doses. The activity of NF-?B increased in the cell nucleus,but decreased in the cytoplasm after exposure to PM2.5 with a significant dose-dependent manner(P

Effect of Sodium Selenite Intervention on the Activity of NF-?B in Renal Tissue of Diabetic Model Rats / 中国药房

OBJECTIVE:To explore the effect of sodium selenite intervention on the activity of NF-?B in renal tissue of rats with different stages of diabetes mellitus(DM).METHODS:SD rats were randomly divided into these groups after the diabetics model were established successfully:DM2 group(DM rats at 2 months),DM4 group(DM rats at 4 months),DM+Se2 group(Se-treated DM rats at 2 months),DM+Se4 group(Se-treated DM rats at 4 months),and set up CON2 group(normal control group at 2 months)and CON4 group(normal control group at 4 months)stimultaneously.All these groups were treated by corresponding methods after feeding different diets for 2 or 4 months,UAER was computed;Scr,BUN,Ucr and the activity of NF-?B in renal tissue were detected.RESULTS:UAER level and activity of NF-?B in DM+Se2 group were all higher than in CON2 group(P

The Effect of Kakkonein on the Expression of NF-?B after Hepatic Ischemia Reperfusion Injury / 医学研究杂志

Objective To investigate the effect of kakkonein on the expression of NF-?B after hepatic ischemia reperfusion injury in rats.Methods Male Sprague-Dawley rats were randomly divided into three groups:pseudo-operation normal control group,hepatic ischemia reperfusion injury model group,kakkonein group.After hepatic ischemia and reperfusion,we analyzed the expression of NF-?B by the methods of Western blot and RT-PCR.Results After hepatic ischemia reperfusion,the NF-?B level increased obviously comparing with the normal group.Comparing to the hepatic ischemia reperfusion injury model group,the NF-?B level decreased after treated with kakkonein.Conclusion The apigenin has a prominent protective effect on hepatic ischemia reperfusion injury in rats through regulating the expression of NF-?B.

Research of the Role about NF-?B in Pathogenesis of Endometriosis / 医学研究杂志

Objective To obtain the role about NF-?B in pathogenesis of endometriosis.Methods We used LPS and LPS+PDTC to intervene normal,eutopic and ectopic endometriotic cells.After collecting cells,we detected the density of NF-?B with WestBlot.Results(1)Comparing with the eutopic and ectopic endometriotic cells,protein NF-?B was the lest expression in the endometrial cells.The difference had uncommonly statistical significance(P

The Influence of LPS and PDTC on IL -1? Concentration in Endometriai Cell Culture Supernatant / 医学研究杂志

Objective To learn the influence of density of LPS and LPS + PDTC on the concentration of IL -1? of endometrial cells. Method We used LPS and LPS + PDTC to intervene normal and endometriosis patients, and obtain cultivate supernatant,then detected the concentration density of IL - 1?with ELISA. Result The of IL - 1? had no statistical signifiance in normal endometrial cells, but there was notable change in eutopic and ectopic endometrial cells. Conclusion Activating and refrainning NF -?B through LPS and PDTC, then decteting the change of the ancentration of IL - 1?, understanding the relation between NF -?B and endometriosis are all important in pathogenesis.

Activity of NF-?B in patients after cardiopulmonary resuscitation and its response to Ulinastain / 中华急诊医学杂志

Objective To investigate the incidence of systemic inflammatory response syndrome(SIRS) after cardiopulmonary resuscitation(CPR) and to observe the effect of Ulinastain in inhibition of inflammatory mediator.Methods Forty patients surviving more than 48 hours after CPR were divided into Ulinastain and control groups randomly. Activity of nuclear factor kappa B(NF-?B), IL-6,TNF-? of the patients was detected .All patients were evaluated by SIRS diagnosis standard and their general organ function was examined. All data were compared between two groups.Results Activities of NF-?B, IL-6,TNF-? of patients after CPR was significantly higher than that of normal people (P

Effects and mechanisms of low-dose hydrocortisone on brain dysfunction in LPS- induced sepsis in rats / 中华急诊医学杂志

Objective To investigate the effects of low-dose hydrocortisone on brain dysfunction in LPS-induced sepsis rats and the role of nuclear factor kappa B (NF-?B) signal transcription pathway in the pathogenesis. Methods Fifty-four rats were randomly divided into 3 groups: control group (A group, n=6), model group (B group, n=24), low-dose HC treatment group(C group, n=24). The septic rat model was established by intra-peritoneal injection of LPS (1 mg/kg), the intervention was by caudal vein injection of low-dose HC (6 mg/kg), each of B and C group was subdivided into 2,8,16,24 hours after LPS injection (n=6).At serial time points,the animals in each group were sacrificed , brain tissue samples were harvested to determine NF-?B,I?B expression in hippocampus by immunohistochemistry. Also, the changes of behaviors were observed and the histopathological changes were investigated by Nissl stain.Results In model group (B group): lethargy, anorexia, tachypnea, piloerection, loss of body weight were all more obvious than control groups.Nissl stain showed significant histopathological changes; NF-?B expression was up regulated compared with control group(P

Effects of Electroacupuncture on the Neuronal Apoptosis and the Expression of NF-?B Protein in Cerebral Ischemia Area of Cerebral Ischemia-reperfusion Rats / 中国中医药信息杂志

Objective To observe the effect of electroacupuncture (EA) on the expression of NF-?B in cerebral ischemia area of cerebral ischemia-reperfusion rats and the neuron apoptosis in cortex by EA on shuigou and neiguan (left) point, and study the protective effect and its mechanism of EA on the cerebral ischemia. Method Cerebral ischemia reperfusion model was established by occlusion of the middle cerebral artery for 30 min and then removal, then EA shuigou and neiguan left points of experimental rats. Neuronal apoptosis in cortex was observed by the terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling (TUNEL), NF-?B protein in the ischemia cortex was detected by immunohistochemistry. Result The neuronal apoptosis and the expression of NF-?B protein in the ischemia cortex was deteded of ischemia side of EA treatment groups were lower comparing with model group. Conclusion EA can lighten the neurons injury after cerebral ischemia-reperfusion by decreasing the expression of NF-?B protein and the neuronal apoptosis in the ischemia cortex.

Experimental Study on the Anti-inflammation Effect of 3,4-oxo-isopropylidene-shikimic Acid / 中国中医药信息杂志

Objective To study the effect of 3,4-oxo-isopropylidene-shikimic acid (ISA) on transcription factor STAT1, STAT3 and NF-?B in human cervix cancer Hela cell. Methods The cell proliferation was assessed by MTT assay. The luciferase activity of transcription factor STAT1, STAT3 and NF-?B was determined by the Dual-Luciferase Reporter Assay System. Results ISA can not inhibited the growth of Hela cell. The luciferase activity of transcription factor NF-?B in Hela cells treated with ISA was inhibited, while the luciferase activities of transcription factor STAT1 and STAT3 were not inhibited. Conclusion ISA can inhibit inflammation, which may be related with suppression of NF-?B transcriptional activity.