Processing Technology Research of Fermentation and Purification of SUMO Protease UlP1 / 中国生物工程杂志

Nowadays,small peptides are always expressed in the form of fusion protein.The expression product contains many superfluous amino acids which can affect the biological functions of small peptides even expressed by GST fusion protein expression system.SUMO protease can cut SUMO fusion protein expressed by fusion expression system without any amino acid residues left on target protein thus become a hot topic in this field.Recombinant His-UlP1/pET3c/BL21(DE3)engineering strain was constructed by genetic engineering technology and the expression conditions were optimized in shake flaks.The process of high density fermentation was explored and different purification conditions were detected by chromatography.The results showed that SUMO protease could be expressed well after inducing the engineering strain by IPTG of 1.0mmol/L at 30℃ for 6 hours.The expression level of the strain in fermentation pots could reach 24.3% analyzed by SDS-PAGE.The purity of SUMO protease was more than 98% after further purification by cation exchange chromatography.The yield was 355mg SUMO protease per liter fermentation liquid.Western blot analysis demonstrated that there were immune reactions between IlP1 and 6?His antibodies,so it has established a good foundation for large-scale industralazation in the future.

A Successful Case of Open Stent-Grafting for an Impending Ruptured Acute Type B Aortic Dissection / 日本心臓血管外科学会雑誌

A 70-year-old man was admitted suffering from chest and back pain. He was assessed by enhanced computed tomography (eCT) and a thrombosed acute DeBakey type IIIb aortic dissection with an ulcer like projection (ULP) was diagnosed and treated medically. Five days later, he complained suddenly of dyspnea and was diagnosed by eCT as having a pulmonary thromboembolism. Anticoagulant therapy was started reluctantly. The patient's symptoms improved, however, 16 days later he complained of severe chest and back pain. Enhanced CT showed enlargement of the ULP, which was diagnosed as an impending aortic rupture. Open stent-grafting was selected as a less-invasive treatment method. A stent-graft was introduced into the descending aorta via the transected aortic arch and the entry of the ULP was closed. Postoperative course was smooth and uneventful. We consider that open stent-grafting via the aortic arch is an alternative method for repair of acute type B aortic dissection with an ULP in the descending aorta, in cases where direct closure of the intimal tear is difficult.

A Case of Surgical Treatment of Stanford Type A Closing Aortic Dissection with Variable Morphological Changes / 日本心臓血管外科学会雑誌

A 62-year-old woman presented with acute chest pain. An enchanced CT scan showed type A closing aortic dissection. An ulcer-like projection (ULP) was observed in the abdominal aorta above the superior mesenteric artery on aortography. At 3 months after onset, recurrent chest pain appeared. An enchanced CT scan showed a false lumen in the ascending aorta and a new ULP and localized false lumen were opacified in the distal ascending aorta on aortography. The graft replacement of the ascending aorta was performed using open distal anastomosis under circulatory arrest and retrograde cerebral perfusion. Two intimal tears were found in the aortic root and distal ascending aorta. The patient recovered without complications. Postoperative CT scan and aortography revealed no residual false lumen.