ABSTRACT
This study represents a comprehensive evaluation of normative values for lymphocyte immunophenotype subsets using flow cytometry techniques in a Japanese population. Lymphocyte reference ranges were determined for percentage and absolute count of T, B, and NK cells in healthy adult Japanese using an extensive two-color immunophenotyping panel and consistently applied quality control methodology. Reference values were also determined for activation markers on CD3+ lymphocytes CD3+/CD25+, CD3+/CD38+ and CD3+/HLA-DR+. Differences in age and gender were observed for specific lymphocyte subsets. Comparison of the Japanese study with a Thai multi-center study that used similar methodology also demonstrated ethnic differences in lymphocyte reference ranges. The results in this study strongly suggest that reference values derived from studies in one population may not be applied to another population even when similar protocols for reagents, instruments and procedures are used although such studies do appear useful for epidemiological comparisons.
Subject(s)
ADP-ribosyl Cyclase/blood , Adult , Age Factors , Antigens, CD/blood , ADP-ribosyl Cyclase 1 , Antigens, Differentiation, B-Lymphocyte/blood , Antigens, Differentiation, T-Lymphocyte/blood , B-Lymphocytes/metabolism , Biomarkers/blood , Female , Flow Cytometry , HLA-DR Antigens/blood , Humans , Immunophenotyping , Japan , Killer Cells, Natural/metabolism , Lymphocyte Activation , Lymphocyte Count , Male , Membrane Glycoproteins , Middle Aged , Receptors, Interleukin-2/blood , Reference Values , Sex Factors , T-Lymphocytes/metabolismABSTRACT
A longitudinal study of lymphocyte subsets during infancy was evaluated by using the flow cytometric immunophenotyping method. Two hundred and thirteen blood samples were obtained from 92 healthy, full-term infants of the following ages: 1-7 days old (n = 43), 3 months old (n = 55), 6 months old (n = 57) and 11 months old (n = 58). The absolute numbers of CD3+ and CD3+/CD4+ T lymphocytes increased from birth to 3 months of age, and remained stable thereafter. The absolute number of CD3+/CD8+ T lymphocytes increased from birth to 11 months of age. The absolute number of CD19+ B lymphocytes and NK cells increased rapidly (3 months) after birth and continued to increase throughout the study period. However, the changes in the relative counts of lymphocyte subsets did not always correspond with the changes in their absolute numbers. These results demonstrate the age-related changes in lymphocyte subpopulations and provide reference ranges for lymphocyte subsets during infancy.
Subject(s)
Age Factors , Antigens, Differentiation, B-Lymphocyte/blood , Antigens, Differentiation, T-Lymphocyte/blood , Female , Follow-Up Studies , Gestational Age , Humans , Infant , Infant Welfare , Infant, Newborn , Killer Cells, Natural/immunology , Longitudinal Studies , Lymphocyte Count , Lymphocyte Subsets/immunology , Male , Reference Values , Sex Factors , T-Lymphocytes/immunology , TaiwanABSTRACT
Of late, there has been an increase in the number of acute leukemias coexpressing markers believed to be restricted to a single lineage. Eight patients with ANLL whose blast coexpressed the T cell associated CD7 antibody were identified among 462 consecutive ANLL cases. Seven had FAB defined AML according to morphocytochemical criteria, whereas one patient was classified as MO on the basis of ultrastructural studies. The incidence of CD7 positivity was particularly significant in the less differentiated sub-types MO and M1 compared to other FAB sub-groups. Detailed long term studies will be required to realize their biological and clinical significance.