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Indian J Biochem Biophys ; 1992 Jun; 29(3): 251-4
Article in English | IMSEAR | ID: sea-26335

ABSTRACT

A technique for determining total proton release from purple membrane suspension under steady illumination has been described. Illuminated purple membrane is found to quench the fluorescence life-time of umbelliferone indicating the release of protons in the medium. Besides the "stoichiometric" release of protons from bacteriorhodopsin, there seems to be release of protons from sources other than protonated retinylidene Schiff base moiety also.


Subject(s)
Bacteriorhodopsins/metabolism , Halobacterium salinarum/metabolism , Kinetics , Light , Protons , Spectrometry, Fluorescence/methods , Umbelliferones
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