ABSTRACT
We made fusion protein of fastatin and FIII 9-10, termed tetra-cell adhesion molecule (T-CAM) that can interact simultaneously with alphavbeta3 and alpha5beta1 integrins, both playing important roles in tumor angiogenesis. T-CAM can serve as a cell adhesion substrate mediating adhesion and migration of endothelial cells in alphavbeta3 and alpha5beta1 integrin-dependent manner. T-CAM showed pronounced anti-angiogenic activities such as inhibition of endothelial cell tube formation, endothelial cell proliferation, and induction of endothelial cell apoptosis. T-CAM also inhibited angiogenesis and tumor growth in mouse xenograft model. The anti-angiogenic and anti-tumoral activity of molecule like fastatin could be improved by fusing it with integrin-recognizing cell adhesion domain from other distinct proteins. The strategy of combining two distinct anti-angiogenic molecules or cell adhesion domains could facilitate designing improved anticancer agent of therapeutic value.
Subject(s)
Animals , Humans , Male , Mice , Angiogenesis Inhibitors/chemistry , Antineoplastic Agents/chemistry , Base Sequence , Benzocaine/chemistry , Cell Line, Tumor , Cell Movement , Cell Proliferation , Cells, Cultured , Chloramphenicol/chemistry , DNA Primers , Drug Combinations , Factor VIII/chemistry , Integrin alpha5beta1/physiology , Integrin alphaVbeta3/physiology , Mice, Inbred BALB C , Nitrofurazone/chemistry , Recombinant Fusion Proteins/chemistryABSTRACT
Se desarrolló un método por espectrofotometría UV, que incluyó la extracción previa del producto de degradación de la benzocaína presente en un nuevo ungüento rectal, destinado al tratamiento del brote hemorroidal agudo. El método analítico se validó para el estudio de estabilidad química de este principio activo. Según los criterios estadísticos vigentes, el método resultó lineal, exacto, preciso, sensible y específico para el objetivo con el cual fue diseñado