ABSTRACT
PURPOSE: To evaluate swine vocal fold healing in a period of 30 days after topical mitomycin-C application. METHODS: Twelve swine underwent exeresis of mucosal flaps from the free edge of the anterior third of both vocal folds with a cold instrument (laryngeal scissors). The animals were divided into two groups: EG (Experimental Group), consisting of 6 animals undergoing topical MMC application (4 mg/dL) on the operated area for 4 minutes; CG (Control Group), 6 animals undergoing topical saline solution application on the operated area for 4 minutes. After 30 days, the animals were sacrificed and the larynges were collected and examined for the presence of synechiae as well as a histological immunohistochemical assessment of immature and mature collagen deposition, number of blood vessels and myofibroblasts. RESULTS: Mature collagen deposition in the EG was 452.12 μm² and 1332.31μm² in the CG; immature collagen deposition was 1511.73μm² in the EG and 1020.61μm² in the CG. The number of myofibroblasts was 1.556 in the EG and 3.583 in the CG. The number of blood vessels was 2.565 in EG and 6.917 in the CG. There were no synechiae in the two studied groups. CONCLUSIONS: There was an increase in immature collagen deposition in the experimental group when compared with the control group. There was a decrease in mature collagen deposition in the experimental group when compared with the control group. There was a decrease in the number of myofibroblasts in the experimental group when compared with the control group. A decrease in blood vessels was observed in the experimental group when compared with the control group. There was no synechia formation in either studied group.
OBJETIVO: Avaliar a cicatrização de pregas vocais de suínos utilizando mitomicina-C tópica, em 30 dias. MÉTODOS: Doze suínos foram submetidos à exérese de mucosa do bordo livre do terço anterior de ambas as pregas vocais com instrumental frio (tesoura curva) e divididos em dois grupos: Grupo E (GE), composto por seis animais com utilização tópica de mitomicina-C(4 mg/dL) na área cruenta por quatro minutos e Grupo Controle (GC), composto por seis animais com utilização tópica de soro fisiológico na área cruenta por quatro minutos. Após 30 dias os animais foram sacrificados e coletadas amostras das pregas vocais para avaliação de sinéquias e análise histológica com quantificação da deposição de colágeno maduro e imaturo, quantificação do número de vasos sanguíneos e miofibroblastos, por método imunoistoquímico. RESULTADOS: A deposição do colágeno maduro no GE foi de 452,12 μm² e 1332,31μm² no GC. A do colágeno imaturo foi de 1511,73μm² no GE e de 1020,61μm² no GC. O número de miofibroblastos foi de 1,556 no GE e de 3,583 no GC. O número de vasos sanguíneos foi de 2,565 no GE e de 6,917 no GC. Não houve formação de sinéquias nos grupo s estudados. CONCLUSÕES: No grupo experimento, quando comparado ao controle, houve significativamente aumento da deposição do colágeno imaturo e diminuição da deposição do colágeno maduro, do número de miofibroblastos e de vasos sanguíneos. Não houve formação de sinéquias em ambos os grupos.
Subject(s)
Animals , Female , Male , Laryngeal Mucosa/drug effects , Mitomycin/pharmacology , Vocal Cords/blood supply , Vocal Cords/surgery , Wound Healing/drug effects , Collagen/biosynthesis , Collagen/classification , Fibrin/biosynthesis , Laryngeal Mucosa/pathology , Models, Animal , Neovascularization, Physiologic , Random Allocation , Swine , Tissue AdhesionsABSTRACT
La colágena es una de las proteínas más abundantes del organismo. Junto con los otros componentes de la matriz extracelular (glucoproteínas no colagenosas, proteoglicanos, lamininas, fibronectinas, tromboespondinas, enectina y tenascina) promueve la adhesión celular, activa vías de señales intracelulares, y regula las actividades de varios factores de crecimiento y de otas proteínas. Durante los últimos 20 años se han identificado por lo menos 19 tipos de colágenas genéticamente diferentes, codificadas por más de 30 genes. En esta primera parte revisamos algunos aspectos novedosos sobre colágenas fibrilares, las formadoras de láminas, las que forman estructuras tipo abalorio, y las multiplexinas. Cuando fue posible correlacionamos las anormalidades de estas proteínas colagenosas con la enfermedad resultante
Subject(s)
Collagen/biosynthesis , Collagen/classification , Collagen/genetics , Collagen/chemistry , Collagen/ultrastructure , Connective Tissue Diseases/genetics , Connective Tissue Diseases/physiopathology , Collagen Diseases/genetics , Epidermolysis Bullosa Dystrophica/genetics , Osteogenesis Imperfecta , Ehlers-Danlos Syndrome/geneticsABSTRACT
Sera obtained from 5 cases of burn scars (patients group 1) and cases of burn scars with squamous cell carcinoma (patients group 2) of the skin are analysed for anticollagen antibodies. Anticollagen type VI was demonstrable in the serum of one out of 5 cases in group 1 and 4 out of 6 cases in group 2. Collagen type VI was demonstrable in the scar without carcinoma of one patient whose serum contained anticollagen type VI. It is suggested that collagen type VI in the scar and their immunobiologic reaction may be related to the regulation of tumor development in scar tissue of the skin.