Studies on Besnoitiosis bennetti in miniature donkeys

Besnoitia tissue cysts associated with the skin lesions recovered from the naturally-infected miniature donkeys [Equus asinus] during clinical examination were studied by the light and electron microscopy, as well as histochemically to elucidate the specific morphologic features of the cyst causing this disease. The cyst was differentiated phenotypically from those of other Besnoitia spp. The interpretation of results showed that morphometric attributes of the tissue cysts and the associated pathological changes in these donkeys were due to B. bennetti infection. The findings were confirmed by the phylogenetic analysis based on DNA sequences of the first internal transcribed spacer of nuclear rDNA. The cluster analysis showed that B. bennetti was distinct from all other Besnoitia spp. and positioned B. bennetti with parasites described from Besnoitia besnoiti of cattle and B. tarandi of reindeer. The genetic attributes complemented the morphological criteria and verified the accurate delimitation of the Besnoitia cysts isolated from these donkeys

Infectivity of trichomonas vaginalis pseudocysts inoculated intra-vaginally in mice

In addition to the trophozoite, pseudocyst is another morphological form which is recently identified among genitourinary trichomonads. Although, this pseudocyst is competent to divide, its role in Trichomonas life cycle has not yet been confirmed. In this study the ability of intra-vaginally inoculated T. vaginalis pseudocysts to induce trichomoniasis in infected mice was evaluated in comparison to the trophozoites. Pseudocysts formation was induced by using thermal-freezing cycle method. The infectivity of the pseudocysts was proved by the presence of T. vaginalis parasite in mice's vaginal washes inoculated in vitro. SEM proved that the pseudocysts withstood on vaginal tissue for 72 hours post infection without any morphological changes. Although the histopathological studies using H and E, PAS and cathepsin D stain proved that there were no differences could be found between trophozoites and pseudocysts in onset of infection, but the pseudocyst had higher infectivity and invasive effects than the trophozoite. So, T. vaginalis pseudocyst is an active form that can induce trichomoniasis

Tratamiento quirúrgico laparoscópico del quiste hepático no parasitario / Surgical laparoscopic treatment of non parasitic hepatic cyst

Se presenta la experiencia de siete casos de quiste hepático no parasitario, atendidos en un periodo de siete años, comprendido de 1992 a 1998 en el Hospital Español de Veracruz. Se describen las manifestaciones clínicas, de las cuales la más importantes fue el dolor por compresión sobre los órganos adyacentes, la sensación de tumoración abdominal, y a la exploración, hepatomegalia de grado variable. El diagnóstico se realizó por ultrasonido y se confirmó por tomografía axial computada. El tamaño de los quistes fue en promedio de 14 cm, y se detectaron en 28.57 por ciento de los casos otros quistes adicionales pequeños. El tratamiento quirúrgico consistió en resección de la pared libre del quiste y electrocoagulación del interior de la cápsula residual, mediante técnica laparoscópica. No se presentaron incidentes transoperatorios, ni existió morbilidad o mortalidad relacionada al procedimiento en sí, además en todos los casos la evolución fue satisfactoria. Autores extranjeros, han reportado el tratamiento de esta entidad con técnica laparoscópica y en México, aunque se ha publicado experiencia de algunos autores con técnica abierta, no existe ninguno con cirugía de mínima invasión

Quiste suprarrenal: reporte de un caso y revisión de la literatura / Suprarenal cysts: a case report and revise of the literature

Los quistes suprarrenales son entidades poco frecuentes reportandose para 1991, solo trescientos casos en el mundo y ninguno en latinoamérica. Informamos el caso de una paciente femenina de 18 años de edad quien consulta por dolor en hipocondrio derecho realizandose ultrasonido y tomografía que reporta quiste gigante suprarrenal derecho, que curso con cifras elevadas de cortisol sin evidencias clínicas de actividad, con supresión a 1mg. de dexametasona. Se realizó resección quirúrgica del mismo, sin complicaciones en el acto quirúrgico, con evolución satisfactoria. La pieza de anatomía patológica confirmó el diagnóstico. Se revisa la literatura, se expone el manejo y seguimiento de quistes suprarrenales

Ultrastructure of the cyst wall of S Lindemanni with pathological correlations

Electron microscopy of skeletal muscle biopsy from a case of human sarcocystosis revealed a new cyst type of S. lindemanni. The sarcocyst appeared large having a thick cyst wall with evident septa extending into the cyst and, characteristically, broad branched cauliflower like protrusions extending into the pericystic zone. This cyst type appeared to be highly pathogenic. In addition to the complete myofibrillar lysis of the infected muscle fibre there were delamination of the neighbouring myofibres, disruption of the sarcomeric orientation with diarrangement and bending of the Z bands and loss of the T and L-myofibrillar pattern in the pericystic zone. Features of myositis such as the presence of abundant lysosomal structures, myofibrillar disarray and glycogen formations were detected. These cytopathogenic signs were obviously attributed to the structural criterion of the cyst wall. The findings not only invalidate the concept that pathological conditions associated with human sarcocytosis are accidental, but also stress: the value of electron microscopy in inducing relevant typing of sarcocysts on basis of their morphologically expressed pathogenic properties