ABSTRACT
AIMS: To additionally purify and characterise the anti-RNA virus-directed protein termed p14. MATERIALS AND METHODS: Antiviral assays of p14 against RNA and DNA viruses were carried out and its antigenic similarities with chicken interferon (CIFN) were studied. HPLC-Reverse Phase of p14 was performed to further purify p14. RESULTS: p14 showed antiviral activity against RNA viruses only and not against DNA viruses. It was antigenically distinct from CIFN. Purification of p14 yielded three proteins with antiviral activity, which had different physico-chemical properties than those described for interferons. CONCLUSIONS: The data presented on the antiviral, immunological and physico-chemical properties, establish the unique nature of p14 vis-á-vis those of interferons.
Subject(s)
Animals , Cells, Cultured , Chick Embryo , Chromatography, High Pressure Liquid , DNA Viruses/drug effects , Helminth Proteins/isolation & purification , Muscle Proteins/isolation & purification , RNA Viruses/drug effects , Sensitivity and SpecificityABSTRACT
Studies were conducted to determine the antiviral activities of synthetic [Troamantadine hydrochloride] and a plant extracts [a commonly used oil extract of Nigella satsiva. and Ukrain] on Herpes simplex virus type-l [DNA] and Polio virus type-l [RNA] virus and this required the determination of the toxicity level of these substances on cell culture and detection of the antiviral activity on tissue culture [Vero cells]. Data indicated that Ukrain is effective on Polio virus because it has significant value as antiviral agent after adsorption on vero cells, but Nigella has a virucidal effect on polio virus. At the same time, Nigella and Troamantadine have a virucidal effect on Herpes virus, but the Troamantadine hydrochloride gave a significant effect as antiviral agent after adsorption of Herpes virus [extra cellular and intra cellular]