ABSTRACT
Administration of single dose (175 mg/kg body wt) of amiodarone dissolved in water through gavage for 3 weeks damaged the lung and changed the content of lung lavage. Activities of bronchoalveolar lavage (BAL) angiotensin converting enzyme (ACE) and lactate dehydrogenase (LDH) increased significantly. Also, the protein and lactate content of the lavage fluid increased significantly over the control. The drug also produced marked changes in morphology of the lung of experimental animals.
Subject(s)
Amiodarone/toxicity , Animals , Bronchoalveolar Lavage Fluid/chemistry , L-Lactate Dehydrogenase/biosynthesis , Lactates/biosynthesis , Lactic Acid , Lung/drug effects , Male , Rats , Rats, WistarABSTRACT
The rates of pyruvate utilization and lactate formation were measured in lymphocytes obtained from mesentéric lymph nodes were incubated at-C for 1 h. The conversion of pyruvate to lactate accounted for 92.5% of the total pyruvate utilized by lymphocytes from yung rats and was 34.2% and 44.5% for adult and aged rats, respectively. Wen lymphocytes were stimulated with concanavalin. A there was an increase of 65.5% and 37.1% in the rates of pyruvate utilization by lymphocytes from young and adult rats, respectively. No changes in pyruvate utilization were observed in response to concanavalin A in lymphocytes from aged rats