ABSTRACT
OBJETIVOS: CD5 é um marcador normalmente expresso nas células T e de forma aberrante nas células B da leucemia linfocítica crônica (LLC) e no linfoma de células do manto (LCM). Outras doenças linfoproliferativas crônicas como a hairy cell leukemia (HCL) e leukemia prolinfocítica de células B (LPL-B), são geralmente CD5 negativas ou expressam fracamente este antígeno. Neste trabalho investigou-se o padrão de expressão do CD5 em 42 pacientes com doenças linfoproliferativas crônicas de células B (DLC-B). METODOS: Investigamos a expressão de CD5 em células leucêmicas de 42 pacientes com DLC-B através da citometria de fluxo. Dados demográficos, tais como idade e sexo, bem como dados clínicos e laboratoriais também foram analisados. RESULTADOS: A imunofenotipagem mostrou que 35 casos foram LLC, 3 LPL-B, 3 HCL e um caso de LMC. O CD5 mostrou-se fortemente expresso em todos os casos de LLC e LMC. Baixa expressão desse antígeno foi observada em um caso de LPL-B, mostrando-se negativamente expresso em todos os casos de HCL. CONCLUSÃO: Nossos resultados demonstram que o padrão de expressão do CD5 pode auxiliar na distinção entre LLC da HCL e LPL-B, sendo no entanto similares na HCL e LCM.
Subject(s)
Aged , Aged, 80 and over , Female , Humans , Male , Middle Aged , /blood , Flow Cytometry/methods , Immunophenotyping , Leukemia, Lymphocytic, Chronic, B-Cell/diagnosis , Lymphoma, B-Cell/diagnosis , Lymphoproliferative Disorders/diagnosis , Diagnosis, Differential , Lymphocyte Count , Leukemia, Hairy Cell/blood , Leukemia, Hairy Cell/diagnosis , Leukemia, Lymphocytic, Chronic, B-Cell/blood , Lymphoma, B-Cell/blood , Lymphoma, Mantle-Cell/blood , Lymphoma, Mantle-Cell/diagnosis , Lymphoproliferative Disorders/blood , Biomarkers, Tumor/bloodSubject(s)
Humans , Flow Cytometry , Leukemia, Hairy Cell , Analysis of Variance , Antibodies, Monoclonal , Antineoplastic Agents/therapeutic use , Case-Control Studies , Chi-Square Distribution , Cladribine/therapeutic use , Follow-Up Studies , Immunohistochemistry , Interferon-alpha/therapeutic use , Leukemia, Hairy Cell/blood , Leukemia, Hairy Cell/drug therapy , Neoplasm, Residual/diagnosis , Neoplasm, Residual/pathology , Recurrence , Remission Induction , Sensitivity and SpecificityABSTRACT
Nine consecutive patients with HCL seen over a period of five years were reviewed. Male: Female ratio was 8:1. Median age at diagnosis was 49 years. Weakness and fatigue (66%) were the commonest presenting symptoms and splenomegaly (66%) was the commonest physical findings. Varying degrees of pancytopenia was the consistent feature in majority of cases. Diagnosis was made on the basis of bone marrow biopsy and characteristic EM picture. Forty-four percent of cases developed serious infection during their clinical course. Gram negative bacilli and fungi were the most frequently isolated organisms. Major sites of infections were pneumonia and septicemia. Splenectomy was carried out in four cases. Rapid recovery of haematological parameters without any significant complication was observed in all these cases. Two patients were treated with alfa-interferon. In both the cases recovery of haematological parameters was slow compared to those under going splenectomy. One patient treated with alfa-interferon died due to infection related complications while the other went into remission.