ABSTRACT
ABSTRACT Objective: To compare the enzyme activity of different presentations of papain solution to validate in-house preparations. Methods: Two papain solutions were prepared, and the third presentation was a commercial solution. Tests were carried out with samples of red cells typed as weak RhD. Results: In-house prepared papain solutions showed similar enzyme reactivity, and statistically no differences compared to the enzyme activity of the commercial solution. Conclusion: Evaluating the cost-benefit ratio, the in-house prepared papain solutions present more economic advantages, and can be incorporated into immunohematological routines as a way to cope with periods of financial crisis and cost-containment policies.
RESUMO Objetivo: Comparar a atividade enzimática de diferentes apresentações de solução de papaína para validação de preparados in-house. Métodos: Foram preparadas duas soluções de papaína, e a terceira apresentação tratou-se de uma solução comercial. Os testes comparativos das reações enzimáticas foram realizados com amostras de hemácias tipadas como RhD fraco. Resultados: As soluções de papaína preparadas in-house apresentaram reatividade enzimática semelhante e estatisticamente sem diferenças em comparação com a atividade enzimática da solução comercial. Conclusão: Avaliando-se a relação entre custo e benefício, as soluções de papaína preparadas in-house são economicamente vantajosas, podendo ser incorporadas às rotinas imuno-hematológicas como forma de enfrentamento em períodos de crise financeira e em políticas de retenção de gastos.
Subject(s)
Humans , Peptide Hydrolases/chemistry , Solutions/standards , Papain/chemistry , Erythrocytes/enzymology , Hematologic Tests/standards , Peptide Hydrolases/economics , Rh-Hr Blood-Group System/economics , Rh-Hr Blood-Group System/chemistry , Solutions/economics , Time Factors , Agglutination Tests/methods , Papain/economics , Reproducibility of Results , Hematologic Tests/economicsABSTRACT
Chemomechanical caries removal (CMCR) is a noninvasive technique eliminating infected dentine via a chemical agent. Th e objective of CMCR is to eliminate the outer layer or infected dentin, leaving the aff ected layer or partly demineralized dentin, which can be remineralized and repaired. As this process based on minimally invasive dentistry, it not only removes infected tissues, also preserves healthy dental structure, avoiding pulp irritation and patient discomfort. Th is is a method of caries removal based on dissolution. Instead of drilling, this method uses a chemical agent assisted by an a traumatic mechanical force to remove soft carious structure. Th e chemomechanical method for caries removal is most outstanding among other alternative methods. Th is paper reviews one of the chemomechanical removal agent, Papacarie.
Subject(s)
Dental Caries/drug therapy , Dental Caries/therapy , Dental Cavity Preparation/methods , Dental Pulp Capping/methods , Dentin/drug effects , Dentin/microbiology , Dentin/therapy , Humans , Materials Testing , Papain/administration & dosage , Papain/analogs & derivatives , Papain/chemistry , Papain/therapeutic useSubject(s)
Cysteine Endopeptidases/chemistry , Enzyme Precursors/chemistry , Plant Proteins/chemistry , Protein Structure, Secondary , Amino Acid Chloromethyl Ketones/chemistry , Amino Acid Sequence , Cathepsin B/chemistry , Computer Simulation , Crystallography, X-Ray , Cysteine Endopeptidases/genetics , Cysteine Proteinase Inhibitors , Enzyme Activation , Enzyme Precursors/genetics , Fruit , Hydrogen Bonding , Leucine/analogs & derivatives , Leucine/chemistry , Leupeptins/chemistry , Models, Molecular , Molecular Sequence Data , Mutation , Oligopeptides/chemistry , Papain/chemistry , Plant Proteins/genetics , Protein Processing, Post-Translational , Sequence Homology, Amino AcidABSTRACT
This study investigated the influence of a papain-based gel(Papacárie) for chemo-mechanical caries removal on bond strength todentin. Human molars were assigned to the following groups: Group 1:sound teeth were flattened to expose dentin; Group 2: after flattening ofsurfaces, the papain-based gel was applied on the sound dentin; Group3: overlying enamel from carious teeth was removed and mechanical excavationof dentin was conducted; Group 4: chemo-mechanical excavationof carious dentin was conducted using the papain-based gel. ThePrime&Bond NT or Clearfil SE Bond adhesive systems were used forrestorative procedures. A microtensile bond strength test was performed,and the modes of failure were determined under SEM. The data weresubmitted to two-way ANOVA and Tukeys test (p ≤ 0.05). No significantdifferences were observed between the sound dentin groups. Forboth excavation methods, Clearfil presented a significantly higher bondstrength than Prime&Bond NT. Also, for Clearfil, the mechanically excavatedsamples disclosed a significantly higher bond strength than thechemo-mechanically ones. For Prime&Bond NT, no significant differenceswere detected between the excavation methods. Predominance ofmixed failures for the sound substrate and of adhesive failures for thecarious dentin one was detected. The bond strength to carious dentinof the self-etching system was negatively affected by chemo-mechanicalexcavation using the papain-based gel.
Subject(s)
Humans , Dental Bonding/methods , Dental Caries/therapy , Dental Cavity Preparation/methods , Dentin-Bonding Agents/chemistry , Dentin/drug effects , Papain/therapeutic use , Adhesiveness , Analysis of Variance , Dental Restoration, Permanent/methods , Dentin-Bonding Agents/pharmacology , Dentin/chemistry , Gels , Materials Testing , Papain/chemistry , Surface Properties , Tensile StrengthABSTRACT
We describe the changes in peptide composition by SDS-PAGE analysis of latex from Carioca papaya collected at various times after incision of the unripe fruit. The data show that during latex coagulation several peptides are processed in an orderly fashion.