ABSTRACT
In view of the need to enhance the functions of Influenza Laboratories in the Region, an assessment of the existing capacity and gaps have been conducted in the Eastern Mediterranean Region recently. Currently, 16 National Influenza Centres [NICs] in 15 Member States of the Region and Influenza Laboratories in seven countries, namely -Djibouti, Libya, Palestine, Saudi Arabia, Somalia, UAE and Yemen have been included in this assessment
Subject(s)
Humans , Orthomyxoviridae/pathogenicity , Influenza, Human/genetics , Respiratory Syncytial Viruses/growth & development , Influenza, HumanABSTRACT
Mink lung epithelial cells (Mv-1-Lu) were tested for their ability to support the growth and serial passage of respiratory syncytial virus (RSV) in vitro. Indian isolates of RSV induced distinctive cytopathic effect with typical rounding of cells followed by detachment with more than 50 per cent cells showing bright fluorescence using anti-RSV monoclonal antibodies in immunofluorescence test. Serial passage of RSV was possible in Mv-1-Lu cells without loss of sensitivity of the cells for virus growth. Titration of cell associated virus and virus released in the supernatant indicated that 60 per cent of the virus was released in the supernatant, and 40 per cent remained cell associated. Transmission electron microscopic studies of negatively stained RSV particles and ultra-thin sections of RSV infected Mv-1-Lu cells showed roughly spherical particles with club shaped projections, budding from the cytoplasmic membrane. These results indicate that Mv-1-Lu cell line is suitable for the growth and propagation of RSV.
Subject(s)
Animals , Cell Line , Cell Size , Child, Preschool , Cytopathogenic Effect, Viral , Female , Humans , India , Infant , Male , Mink , Respiratory Mucosa/cytology , Respiratory Syncytial Viruses/growth & development , Virus CultivationABSTRACT
DOT ELISA was compared with RT-PCR and tissue culture to detect RSV from nasopharyngeal aspirates. DOT ELISA had diagnostic sensitivity and specificity of 65.62% and 93.92%, respectively. The results indicate that DOT ELISA can be used for screening detection of RSV from clinical specimens and is suitable for small laboratories in the provincial areas of developing countries.
Subject(s)
Cells, Cultured , Child , Enzyme-Linked Immunosorbent Assay/methods , Humans , Infant , Polymerase Chain Reaction/methods , Respiratory Syncytial Virus Infections/diagnosis , Respiratory Syncytial Viruses/growth & development , Sensitivity and SpecificitySubject(s)
Humans , Child , Bronchiolitis/classification , Bronchiolitis/complications , Bronchiolitis/diagnosis , Bronchiolitis/drug therapy , Bronchiolitis/epidemiology , Bronchiolitis/etiology , Bronchiolitis/mortality , Bronchiolitis/physiopathology , Bronchiolitis/therapy , Respiratory Syncytial Viruses/growth & development , Respiratory Syncytial Viruses/isolation & purification , Respiratory Syncytial Viruses/pathogenicity , Respiratory Syncytial Viruses/physiologyABSTRACT
Las infecciones respiratorias agudas son una de las principales causas de morbilidad y mortalidad entre los niños. Con el desarrollo de tecnologías de diagnóstico rápido para la detección de antígenos virales es posible reconocer el agente viral de la infección respiratoria en horas. El diagnóstico etiológico de infección respiratoria viral es no sólo cada vez más importante para la selección apropiada de los pacientes que deben recibir tratamiento antiviral o con antibióticos, sino también para el control de la diseminación de las infecciones respiratorias virales en salas pediátricas. En la Clínica Amparo Infantil Santa Ana de Medellín ocurrió un brote de infección respiratoria aguda del tracto respiratorio inferior en el último trimestre de 1994 producida por virus. Los virus detectados fueron virus respiratorio sincitial 41.8 por ciento, adenovirus 33,3 por ciento, parainfluenza tipo 1, en el 8.3 por ciento e infección mixta en el 16.7 por ciento. Se describe el método diagnóstico utilizado en la detección de los antígenos virales y las características de este brote.
Subject(s)
Humans , Child , Bronchiolitis, Viral/classification , Bronchiolitis, Viral/diagnosis , Bronchiolitis, Viral/drug therapy , Bronchiolitis, Viral/epidemiology , Bronchiolitis, Viral/etiology , Bronchiolitis, Viral/nursing , Pneumonia, Viral/classification , Pneumonia, Viral/diagnosis , Pneumonia, Viral/nursing , Pneumonia, Viral/etiology , Adenoviridae Infections , Adenovirus Infections, Human/classification , Adenovirus Infections, Human/diagnosis , Adenovirus Infections, Human/nursing , Parainfluenza Virus 1, Human/classification , Parainfluenza Virus 1, Human/growth & development , Parainfluenza Virus 1, Human/isolation & purification , Respiratory Syncytial Viruses/classification , Respiratory Syncytial Viruses/growth & development , Respiratory Syncytial Viruses/isolation & purificationABSTRACT
El presente trabajo, se realizó en el laboratorio de Virología del Instituto Nacional de Enfermedades Respiratorias, México, D.F. Los objetivos fueron: encontrar las condiciones óptimas para la fijación y tinción de las placas virales producidas por los virus herpes simple tipo 1 y sincitial respiratorio: Ambos virus, fueron propagados en monocapas de células Vero y se observaron a diferentes tiempos. Para la fijación de las monocapas infectadas se probaron cinco sustancias: formaldehído, etanol, metanol, acetona y éter-metanol y se tiñeron con tres colorantes: Giemsa, Wrigth y cristal violeta. Los mejores resultados se obtuvieron al usar éter al 5 por ciento en metanol como fijador y Giemsa como colorante. El tiempo más corto en que se observó el efecto citopático fue: 18 h para el virus sincitial respiratorio y 15 h para el virus herpes simple. por lo que el uso de estas sustancias, facilitó la visualización del efecto citopático en tiempos breves