ABSTRACT
Objective: to verify selenium effectiveness in maintenance of prostate tissue architecture. Method: experimental study using 20 adult 90-day-old male rats divided into the following groups: TG, 05 animals that received injectable testosterone; TSG, 05 animals that received injectable testosterone and weekly doses of selenium by gavage; CG1, 05 intact animals; CG2, 05 animals that received saline injection and saline by gavage. Results: characteristic architecture was found in tissue samples from animals of CG with cubic/prismatic secretory epithelium surrounded by fibro-muscular stroma. Animals of TG showed an increase in prostatic epithelium height, increase in the number of blood vessels in stroma and presence of proliferative lesions. Proliferative lesions were also found in tissue samples from animals of TSG, besides having improve in epithelial height, as seen in TG. Conclusion: it is concluded that selenium at this concentration has no effectiveness in modulating morphology of prostatic tissue of adult rats.(AU)
Objetivo: verificar la eficacia del selenio en mantener la morfología tisular. Método: estudio experimental, utilizando 20 ratones machos adultos (5 animales/grupo), divididos en: TG, recibieron testosterona inyectable; TSG, recibieron testosterona inyectable y dosis semanales de selenio por gavado; CG1, animales intactos; CG2, recibieron solución salina inyectable y por gavado. Resultados: una arquitectura característica fue encontrada en las muestras tisulares de los animales del CG, con epitelio cúbico/prismático envuelto por estroma fibro-muscular. En los animales del TG la próstata presentó un epitelio con células más altas, un aparente aumento en el número de vasos sanguíneos estromais, además de la presencia de lesiones proliferativas. También se encontraron lesiones proliferativas en las muestras tisulares de los animales del TSG, además de presentar mayor altura del epitelio, como vistas en TG. Conclusión: se concluye así que el Selenio, en esta concentración, parece no ser eficaz en la protección contra las modificaciones promovidas por la administración de T exógena en ratas adultas.(AU)
Objetivo: verificar a eficácia do selênio na manutenção da morfologia tecidual prostática. Métodos: estudo experimental, utilizando 20 ratos machos adultos, divididos em: TG, 05 animais que receberam testosterona injetável; TSG, 05 animais que receberam testosterona injetável e doses semanais de selênio por gavagem; CG1, 05 animais intactos; CG2, com 05 animais que receberam solução salina injetável e por gavagem. Resultados: uma arquitetura característica foi encontrada nas amostras teciduais dos animais do CG, com epitélio cúbico/prismático envolvido por estroma fibro-muscular. Nos animais do TG a próstata apresentou um epitélio com células mais altas, um aparente aumento no número de vasos sanguíneos no estroma, além da presença de lesões proliferativas. Também foram encontradas lesões proliferativas nas amostras teciduais dos animais do TSG, além de apresentarem maior altura do epitélio, como vistas no TG. Conclusão: conclui-se assim, que o Selênio, nesta concentração, parece não ser eficaz na proteção contra as modificações promovidas pela administração de T exógena em ratos adultos.(AU)
Subject(s)
Animals , Rats , Prostate/anatomy & histology , Testosterone/administration & dosage , Selenium Compounds/administration & dosage , Clinical Trial , Rats, WistarABSTRACT
The field of nanotechnology is rapidly expanding .The development quantum dots quantum dot [QDs], show great promise for treatment and diagnosis of cancer and targeted drug delivery little data on the toxicity of QDs, especially for in vivo applications, are available. As a result, concerns exist over their toxicity for in vivo applications. Then, cytotoxic effects of cadmium selenide [CdSe] quantum dots on organs development before maturity were studied in this study. One month old male Mice treated by injection of CdSe at the doses of 10, 20 and 40 mg/kg. Structural and optical properties of quantum dots were studied by XRD, UV-Vis absorption spectrum and Scanning Tunneling Microscopy and the number of cells in seminiferous tubes of various groups were analyzed using SPSS 16 program [one way ANOVA test]. Histological studies of testis tissue showed high toxicity of cdse in the dose of 40 mg/kg which followed by decrease in lamina propria thickness, destruction in interstitial tissue, deformation of seminiferoustubes, and reduction in number cells. Also histological study of lung tissue showed in 20 and 40 mg/kg doses destruction in interstitial and epithelium tissues. On the whole, this study showed high toxicity of cdse on development of testis and lung tissues, even in low doses considering lack of literature review in this field, this study can be an introduction to researches about toxicity effect of quantum dots on development of organs
Subject(s)
Animals, Laboratory , Animal Structures/growth & development , Selenium Compounds/toxicity , Cadmium Compounds/toxicity , MiceABSTRACT
No abstract available.
Subject(s)
Rodentia , Selenium , Selenium Compounds , Transplantation, HeterologousABSTRACT
The gamma-cyclodextrin-folate (gamma-CD/FA) inclusion-coated CdSe/ZnS quantum dots (QDs) with folate-receptor (FR) targeted were synthesized by simple and convenient sonochemical method. The products were studied using Fourier transform infrared (FTIR), proton nuclear magnetic resonance (1H NMR), utraviolet-visible spectrometry (UV-vis), fluorescence spectrum and transmission electron micrographs (TEM). The results showed that the gamma-CD/FA-coated CdSe/ZnS QDs not only have good monodispersity and smaller size, but also have good optical performance, such as higher quantum yield (QY) and a long fluorescence lifetime. The cytotoxicity experiments showed that the gamma-CD/FA-coated CdSe/ZnS QDs have lower cytotoxicity and could more effectively enter cancer cells with FR over-expression. The QDs with 4-5 nm in diameter were relatively easy to enter the cell and to be removed through kidneys, so it is more suitable for biomedical applications for bioprobes and bioimaging.
Subject(s)
Humans , Cadmium Compounds , Chemistry , Metabolism , Toxicity , Cell Survival , Folate Receptor 1 , Chemistry , Folic Acid , Chemistry , HeLa Cells , Hep G2 Cells , Magnetic Resonance Spectroscopy , Microscopy, Electron, Transmission , Molecular Imaging , Methods , Quantum Dots , Chemistry , Metabolism , Toxicity , Selenium Compounds , Chemistry , Metabolism , Toxicity , Spectrophotometry, Ultraviolet , Spectroscopy, Fourier Transform Infrared , Sulfides , Chemistry , Metabolism , Toxicity , Zinc Compounds , Chemistry , Metabolism , Toxicity , gamma-Cyclodextrins , ChemistryABSTRACT
<p><b>OBJECTIVE</b>To determined hydrogen selenide in workplace air with atomic fluorescence.</p><p><b>METHOD</b>Hydrogen selenide were sampled with 0.1 mol/L sodium hydroxide solution in multi-hole absorbing tubes. The sampled absorbing solution were digested with (9+1) nitric acid/perchloric acid. The selenide in sample were reduced by potassium borohydride in 5.0% hydrochloride solution and determined with atomic fluorescence.</p><p><b>RESULTS</b>There was a good linearity (r=0.9999) over the concentration of 0-150 microg/L, The precision of low, middle and high concentration were 3.1%, 7.4% and 6.7%, respectively. The sample collection rate can reach 99%.</p><p><b>CONCLUSION</b>The method was accurate and sensitive to detect hydrogen selenide in workplace air.</p>
Subject(s)
Air Pollutants, Occupational , Selenium Compounds , Spectrometry, Fluorescence , Spectrophotometry, Atomic , WorkplaceABSTRACT
<p><b>INTRODUCTION</b>Inflammatory tinea capitis is an uncommon condition in Singapore. In this case report we present a patient whom we managed for this condition.</p><p><b>CLINICAL PICTURE</b>A 4-year-old girl presented to us with multiple pustules over the occipital scalp for 6 weeks, associated with painful cervical lymphadenopathy. Her condition did not respond to topical and oral antibiotics.</p><p><b>TREATMENT</b>The patient was diagnosed with kerion (inflammatory tinea capitis) and fungal culture of plucked hairs from the kerion grew Microsporum species of dermatophyte. She was treated with a course of oral griseofulvin and topical selenium sulfide shampoo. She was advised to bring her pet cats to the veterinarian for screening, as well as not to share combs with her other siblings.</p><p><b>OUTCOME</b>Her condition improved with the antifungal therapy, and there was no residual alopecia.</p><p><b>CONCLUSION</b>Physicians should consider tinea capitis when they encounter a patient with scalp folliculitis or scarring alopecia in the appropriate clinical context.</p>
Subject(s)
Child, Preschool , Female , Humans , Administration, Oral , Administration, Topical , Antifungal Agents , Griseofulvin , Microsporum , Selenium Compounds , Tinea Capitis , Drug Therapy , MicrobiologyABSTRACT
Some selenium compounds offer important health benefits when administered at supranutritional doses, such as improvement of the immune system and of male fertility, and the prevention of some types of cancer. The traditional selenium indexes do not account for the metabolic status of this element among replete individuals. As a consequence, there is a need for new indexes that distinguish between repletion statuses of selenium. The aim of this work was to indentify some plasmatic proteins that respond to supranutritional doses of selenium, which could be proposed as new protein markers of selenium intake. The effect on rats of dietary supplementation with either selenomethylselenocysteine (SMSeC) or sodium-selenate on some blood plasma proteins was investigated. Two experimental groups consisting of six rats each were fed a basic diet supplemented with either SMSeC or sodium-selenate at 1.9 mg-Se / g-diet for ten weeks. The control group was fed a diet that contained the recommended selenium dose (0.15 mg-Se / g-diet). The changes in the abundance of a group of plasmatic proteins were quantified and analysed statistically. Haptoglobin, apolipoprotein E and transthyretin increased their abundance after diet supplementation with either form of selenium. HNF6 was responsive only to SMSeC, whereas fibrinogen responded only to sodium-selenate. We postulate that the protein patterns observed in this work could be proposed as new molecular biology-based markers of selenium intake.
Subject(s)
Animals , Male , Rats , Blood Proteins/drug effects , Cysteine/analogs & derivatives , Dietary Supplements , Organoselenium Compounds/administration & dosage , Selenium Compounds/administration & dosage , Selenium/blood , Blood Proteins/analysis , Cysteine/administration & dosage , Electrophoresis, Gel, Two-Dimensional , Mass Spectrometry , Rats, WistarABSTRACT
We designed a novel microcantilever immuosensor based on magnetic microbead, applying different-sized CdSe QDs as fluorescent probes and polystyrene magnetic microbead. The novel microcantilever immuosensor used fluorescent probes embedded polystyrene microbeads and specific antibodies on the surface of the polystyrene microbead. In addition, we studied the mechanism of the on-chip magnetic separation, the structure of micro-electromagnet and the microbead magnetization by the micro-magnetic field, the snake-shaped planar micro-electromagnet for the novel microcantilever immuosensor.
Subject(s)
Antibodies , Biosensing Techniques , Methods , Cadmium Compounds , Chemistry , Equipment Design , Immunoassay , Methods , Magnetics , Micro-Electrical-Mechanical Systems , Microspheres , Nanotechnology , Polystyrenes , Chemistry , Quantum Dots , Selenium Compounds , ChemistryABSTRACT
La papilomatosis reticulada y confluente (Gougerot-Carteaud) es una entidad infrecuente caracterizada por la presencia de pápulas pardas queratósicas que coalescen formando placas de aspecto reticulado en la periferia. La afectación familiar es extremadamente rara. Se presenta un caso de PRC en 2 hermanos
Subject(s)
Humans , Male , Female , Adolescent , Adult , Papilloma , Selenium CompoundsABSTRACT
A simple and convenient procedure for stereoselective synthesis of (Z)-allyl selenides has been developed by a one-pot reaction of diselenides with Baylis-Hillman adducts in the presence of samarium metal-trimethylsilyl chloride under mild conditions. Presumably, the diselenides are cleaved by Sm/TMSCl system to form selenide anions, which then undergo S(N)2' substitution of Baylis-Hillman adducts to produce the (Z)-allyl selenides.
Subject(s)
Magnetic Resonance Spectroscopy , Molecular Structure , Organoselenium Compounds , Chemistry , Selenium Compounds , Chemistry , StereoisomerismABSTRACT
Epidemiological, clinical and experimental evidence collectively suggests that Se in different inorganic and organic forms provides a potential cancer chemopreventive agent, active against several types of cancer. It can exert preventive activity in all the three stages of cancer: initiation, promotion and progression. Literature reports revealed that organoselenocyanates have more potential as chemopreventive agents than inorganic forms due to their lower toxicity. In our previous report we showed chemopreventive efficacy of diphenylmethyl selenocyanate during the initiation and pre- plus post-initiation phases of skin and colon carcinogenesis process. The present study was undertaken to explore the anti-tumour promoting activity of diphenylmethyl selenocyanate in a 7,12-dimethylbenz (a) anthracene (DMBA)-croton oil two-stage skin carcinogenesis model. The results obtained showed significant (p<0.01) reduction of the incidence and number of skin papillomas, precancerous skin lesions, along with significant (p<0.01) elevation of phase II detoxifying enzymes (GST, Catalase and SOD) and inhibition of lipid peroxidation in liver and skin. Thus, the present data strongly suggest that diphenylmethyl selenocyanate also has the potential to act as anti-tumour promoter agent in a two-stage skin carcinogenesis mouse model, pointing to possible general efficacy.
Subject(s)
9,10-Dimethyl-1,2-benzanthracene , Analysis of Variance , Animals , Anticarcinogenic Agents/pharmacology , Croton Oil , Cyanates/pharmacology , Female , Lipid Peroxidation , Mice , Papilloma/chemically induced , Selenium Compounds/pharmacology , Skin Neoplasms/chemically inducedABSTRACT
La papilomatosis reticulada y confluente (Gougerot-Carteaud) es una entidad poco frecuente que se carateriza por la presencia de papulas pardas queratósicas que coalescen formando placas de aspecto reticulado en la periferia, cuyas localizaciones mas frecuentes son nuca, axilas, tronco anterior y zona intermamaria. Presentamos un caso que respondió favorablemente al tratamiento con sulfuro de selenio. Se realiza una revisión del tema
Subject(s)
Humans , Adult , Female , Papilloma , Selenium , Selenium CompoundsABSTRACT
Selenium, an essential micronutrient, plays important roles against different diseases, including several types of cancer. In the present study, antioxidative and chemopreventive properties of a synthetic organoselenium compound, diphenylmethyl selenocyanate, were evaluated with a 7,12-dimethylbenz (a) anthracene - croton oil induced two-stage mouse skin carcinogenesis model. The compound was administered orally to carcinogen-treated mice at two different non-toxic doses, 2mg/kg. b.w. and 3mg/kg. b.w. Significant inhibition in the incidence of papilloma formation (53-80%) as well as in the cumulative numbers of papillomas per papilloma bearing mouse were observed in the treated groups as compared to the carcinogen control group. The compound was also found to upregulate significantly different phase II detoxifying enzymes such as glutathione-S-transferase (p<0.01) and superoxide dismutase (p<0.01) in skin cytosol when measured after 15 days and also after 12 weeks of the first 7,12-dimethylbenz (a) anthracene treatment. Lipid peroxidation measured with reference to thiobarbituric acid reactive substances in skin microsomes was significantly inhibited (p<0.05) in a dose dependent manner by diphenylmethyl selenocyanate. Considerable inhibition of the level of nitric oxide production in peritoneal macrophages was observed after 12 weeks (p<0.05). Thus the compound appears to exert chemopreventive activity in terms of papilloma formation, which may be through modulation of cutaneous lipid peroxidation, the phase II detoxifying enzyme system and nitric oxide production.
Subject(s)
Analysis of Variance , Animals , Carcinogenicity Tests , Croton Oil , Cyanates/pharmacology , Disease Models, Animal , Dose-Response Relationship, Drug , Drug Administration Schedule , Female , Glutathione Reductase/metabolism , Glutathione Transferase/metabolism , Lipid Peroxidation/physiology , Mice , Mice, Inbred A , Nitric Oxide/biosynthesis , Oxidative Stress , Papilloma/pathology , Probability , Random Allocation , Selenium Compounds/pharmacology , Sensitivity and Specificity , Skin Neoplasms/pathology , Superoxide Dismutase/metabolismABSTRACT
The yeast fusant ZFF-28, which is high in biomass production and rich in selenium, was constructed after mutagenesis and protoplasts fusion between yeast strains. The total selenium content of ZFF-28 is 1.8 and 1.0 times higher than that of the parental strains Saccharomyces cerevisiae ZY-67 and Saccharomyces kluyveri SZY-198 respectively. Using single factor tests and a L16(4(3) x 2(1)) orthogonal design, the cultivation conditions was optimized as: 50mL culture in 250mL shake flasks in molasses containing 6% sugar and 60microg/mL Se at 28 degree C for 25h at 220 r/min, with the initial pH adjusted to 6.0 - 6.5. Under the optimized conditions, the biomass (dry weight) reached 8.2g/L and the Se content of the cells reached 2050microg/g, with organic and inorganic Se contents being 91% and 9% respectively.
Subject(s)
Biomass , Hydrogen-Ion Concentration , Saccharomyces , Genetics , Metabolism , Saccharomyces cerevisiae , Genetics , Metabolism , Selenium , Metabolism , Selenium Compounds , MetabolismABSTRACT
Pot culture studies were conducted to examine the effect of selenite (SeO3(2-)) and selenate (SeO4(2-)) on the uptake and translocation of root absorbed selenium in maize Zea mays plants grown in sand and soil culture. Increasing selenium supplementation (0.5-6.00 microg/ml), increased the selenium retention in roots, but there was little transfer of selenium from shoot to grains. The study indicates that selenite species (less mobile) also accumulates in maize plants when supplied in solution form. Selenium does not cause any adverse effect on the maize plants (dry matter yield vs concentration, no significant correlation, p>0.05).
Subject(s)
Biological Availability , Plant Roots/physiology , Selenium/chemistry , Selenium Compounds/pharmacokinetics , Sodium Selenite/pharmacokinetics , Soil , Tissue Distribution , Zea mays/physiologyABSTRACT
A possible method of controlling the parasitic disease schistosomiasis is to kill the intermediate host using the toxic selenium dioxide on the snails Biomphalaria alexandrina. Selenium dioxide [SeO[2]] has therefore been given to the snails in a soluble form in water Se-H[2]O] or in food attractant pellets [Se-P] in order to study the mode of action of selenium on the snails. In this study, transaminases [ALT and AST], lactate dehydrogenase [LDH], adenosine deaminase, AMP deaminase, alkaline phosphatase [ALP] and 5'-nucleotidase [5'-Nu] activities in snail homogenates have been measured., The application of Se in form of food attractant pellets showed a much greater effect on snails ALP and 5'-Nu activities than its applications directly in water. However LDH activity was affected when snails were incubated in water containing SeO[2] more than feeding the animals food attractant pellets containing Se. In general the highest effect of Se on the snails was found in the activity of 5-nucleotidase. The present studies show that selenium supplemented to the snails in food attractant pellets could be a possible method for controlling the snails. However the toxicity of the food pellets needs to be improved
Subject(s)
Schistosomiasis/parasitology , Selenium , Selenium Compounds , Snails/parasitologyABSTRACT
Several compounds of 1,2,3-selena and/or thioadizoles with evident biological activity were previously reported by the authors, certainly as antibacterial and antifungal agents [1-6] e.g. 1,2,3-thiadiozolo and/or selenadiazolo-4-yl-pheynyl-hydrazophyrazolones [10] were tested in vitro against a variety of Gram positive strains of bacteria. Certain compounds show strong activity against Bacillus sub., staphylococcus areus and Mycobacterium phlei. Thus, pyridine [7], furan [8] and thiophene [9] containing 1,2,3-selena and thiadiazoles motivated their synthesis and testing as biologically active compounds. In this work, compounds [1-3] were easily condensed with semicarbazide to give the corresponding semicarbazones [4-6] respectively. On the other hand, the purified semicarbazones [4-6] were then oxidized either with selenium dioxide [11] in glacial acetic acid to give 1,2,-3-selenadiazole Derivatives[9,11] or with thionyl chloride[12] to afford, 1,2,3-thiadiazole derivatives[10,12]
Subject(s)
Selenium Compounds , Semicarbazides , SemicarbazonesABSTRACT
Selenium dioxide was administered (ip) to albino male rats (200 +/- 5 g) in increasing doses (2, 6 and 10 micrograms/rat, daily) for 90 days. Histologic and histometric study showed that there was dose dependent injury to testes. No cellular deformation was observed following 2 micrograms-dose; whereas 6 micrograms-dose caused significant damage of gametogenic cells. At a dose of 10 micrograms, there was a significant testicular degeneration along with testicular atrophy.