ABSTRACT
Mucosal mast cell activity was quantified by measuring histamine forming capacity [HFC] of the gastric mucosa and histamine content in the intestinal tissues of mice infected with T. spiralis. The results were correlated with the kinetics of worm expulsion. It was found that T. spiralis resulted in significant elevation of HFC by the day 6 post infection [p.i.] which reached a maximal value at day 9, a time when approximately 50% of the established worm burden had been expelled. Histamine content of the intestinal tissues followed the same pattern. No intestinal worms were present by day 28 of infection and there was a gradual reduction in HFC and histamine content which had returned almost to control values by that time. Significant inverse correlation between individual worm burdens and HFC was detected
Subject(s)
Animals, Laboratory , Trichinellosis/physiopathology , Mice , Histamine/metabolism , Mast Cells , Stomach/chemistry , Intestines/chemistryABSTRACT
Se presenta un caso de triquinosis endometrial en una paciente proveniente de la zona de Cd. Delicias, Chih., área endémica de esta parasistosis. Se trató de una mujer de 38 años de edad, obesa, diabética, hipertensa, con antecedente de consumo frecuente de carne de cerdo y su derivados, que cursó con sangrado uterino anormal y síntomas de enfermedad inflamatoria pélvica crónica. Se le practicó histerectomía en el espécimen quirúrgico se identificaron larvas de Trichinella spiralis enquistadas en la zona basal del endometrio, acompañadas de microcalcificaciones e infiltrado linfocitario. Aparentemente esta localización no ha sido descrita previamente
Subject(s)
Adult , Humans , Female , Endometritis/diagnosis , Endometritis/parasitology , Trichinella spiralis/pathogenicity , Trichinellosis/diagnosis , Trichinellosis/physiopathologyABSTRACT
An outbreak of trichinosis occurred in Purranque county, X Region, Chile, between october and november of 1992, which involved 36 persons. The incubation period, determinated by the clinical picture and laboratory assays, fluctuated between 10 and 12 days, myalgias (88,9 for percent) and palpebral oedema (86,1 por percent) were the most important symptoms, followed by fever (44,4 for percent) and headache (33,3 for percent). Eosinophils count ranged from two to 42 for percent the first week, and this value raised to 55 for percent the second week of the outbreak. Anti-trichinella spiralis antibodies were determinated by some serological tests such as: precipitin test (PT), bentonite flocculation test (BFT) and indirect hemagglutination test (IHAT) in sera IHAT (21,4 for percent) and BFT (3,6 for percent). After 15 days, the three tests were perfomed in sera from 12 patients. At that time, the positivity was elevated in all of them: PT (100,0 for percent), BFT (66,7 for percent) and IHAT (91,7 for percent). It is believed that the outbreak had its origin in infected pork meat that was consumed raw or insufficiently cooked without a previous veterinary inspection
Subject(s)
Humans , Male , Female , Child, Preschool , Adolescent , Adult , Middle Aged , Chile/epidemiology , Disease Outbreaks/statistics & numerical data , Trichinellosis/epidemiology , Eosinophils , Health Surveys , Precipitin Tests , Serologic Tests/statistics & numerical data , Signs and Symptoms , Flocculation Tests , Hemagglutination Tests , Immunologic Tests/methods , Trichinella spiralis/isolation & purification , Trichinellosis/diagnosis , Trichinellosis/physiopathologyABSTRACT
In this study, the role of Toxocara canis and Trichinella spiralis infection in changing enzymatic activity that included in tryptophan metabolism via kynurenine pathway, and their effects on the activity of the lysosomal enzyme alpha-naphthyl acetate esterases, was considered. Toxocara canis and Trichinella spiralis produce a significant inhibitory effect on the kynurenine hydrolase. They produce a significant increase in the activity of the free lysosomal alpha-naphthyl acetate esterases in liver. Trichinella spiralis produces a significant inhibitory effect on the kynurenine transaminase. Larvae of the Toxocara canis parasite can remain alive releasing their metabolites in the surrounding tissues for years. As a result of this fact, the inhibitory effect of the Toxocara canis infection on the kynurenine hydrolase led to the suggestion that the accumulated kynurenine may be metabolized to 3-hydroxy kynurenine which is considered as a potent carcinogen. Nematode Trichinella spiralis inhibits kynurenine transaminase and kynurenine hydrolase and may withdraw the tryptophan and metabolize it to serotonin as well as nematode Ascaridia galli which is capable to synthesize 5- hydroxytryptamine from tryptophan via 5-hydroxy tryptophan. The increase in the activity of alpha-naphthyl acetate esterases after infection with both Trichinella spiralis and Toxocara canis larvae may be due to the increased fragility and rupture of lysosomes of the liver cells leading to the leakage of this lysosomal enzymes. Also, the rise in the enzyme activity indicates tissue damage which was attributed mostly to immunological nature. Detection of alpha- naphthylacetate esterases activity in urine and blood of patients infected with these tissue nematodes for long period should not be ignored, also detection and determination of the potent carcinogen 3- hydroxy kynurenine in urine of those patients is of urgent need