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Rev. bras. pesqui. méd. biol ; Braz. j. med. biol. res;46(9): 765-770, 19/set. 2013. tab, graf
Article in English | LILACS | ID: lil-686568

ABSTRACT

Oxidative low-density lipoprotein (Ox-LDL) is a key risk factor for the development of atherosclerosis, and it can stimulate the expression of a variety of inflammatory signals. As a new and highly sensitive inflammation index, OX40L may be a key to understanding the mechanisms that regulate interactions between cells within the vessel wall and inflammatory mediators during the development of atherosclerosis. To investigate whether Ox-LDL regulates OX40L expression through an oxidized LDL-1 receptor (LOX-1)-mediated mechanism, we investigated the effect of different concentrations of Ox-LDL (50, 100, 150 µg/mL) on endothelial cell proliferation and apoptosis. Stimulation with Ox-LDL increased OX40L protein 1.44-fold and mRNA 4.0-fold in endothelial cells, and these effects were inhibited by blocking LOX-1. These results indicate that LOX-1 plays an important role in the chronic inflammatory process in blood vessel walls. Inhibiting LOX-1 may reduce blood vessel inflammation and provide a therapeutic option to limit atherosclerosis progression.


Subject(s)
Humans , Apoptosis/drug effects , Cell Proliferation/drug effects , Human Umbilical Vein Endothelial Cells/drug effects , Lipoproteins, LDL/pharmacology , /metabolism , Scavenger Receptors, Class E/metabolism , Atherosclerosis/etiology , Atherosclerosis/prevention & control , Cell Cycle , Cells, Cultured , Human Umbilical Vein Endothelial Cells/cytology , Human Umbilical Vein Endothelial Cells/metabolism , Immunoblotting , Lipoproteins, LDL/metabolism , Lipoproteins, LDL/physiology , /genetics , Real-Time Polymerase Chain Reaction , Signal Transduction , Vasculitis/physiopathology , Vasculitis/prevention & control
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