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1.
Braz. j. biol ; 83: e251075, 2023. tab, graf
Article in English | LILACS, VETINDEX | ID: biblio-1339401

ABSTRACT

Abstract Plant-derived products can assist in the healing process of dermal wounds. It has been demonstrated that Hancornia speciosa latex present angiogenic, osteogenic, anti-inflammatory, and antioxidant activities. Then, it could contribute to the wound healing process. However, natural products in contact with skin may cause dermatitis. The objective of this work was to evaluate the allergic and irritant potential of H. speciosa serum fraction latex using in vitro assays. The obtained results showed that the H. speciosa serum fraction latex has a slightly irritant potential and is not cytotoxic neither allergenic for human cells. Moreover, we identified a remarkable low amount of proteins in this material in comparison to Hevea brasiliensis latex. This result could explain the non-allergenic potential of H. speciosa serum fraction latex because proteins present in latex are the main responsible for allergy. This biomaterial could be used as a non-allergenic source for development of new medicines.


Resumo Produtos derivados de plantas podem auxiliar no processo de cicatrização de feridas cutâneas. Foi demonstrado que o látex de Hancornia speciosa apresenta atividades angiogênicas, osteogênicas, antiinflamatórias e antioxidantes. Então, este biomaterial pode contribuir para o processo de cicatrização de feridas. No entanto, produtos naturais em contato com a pele podem causar dermatites. O objetivo deste trabalho foi avaliar o potencial alérgico e irritante do látex da fração soro de H. speciosa por meio de ensaios in vitro. Os resultados obtidos mostraram que o látex da fração do soro de H. speciosa possui um potencial pouco irritante e não é citotóxico nem alergênico para células humanas. Além disso, foi identificado uma notável baixa quantidade de proteínas neste material em comparação ao látex de Hevea brasiliensis. Esse resultado poderia explicar o potencial não alergênico do látex da fração soro de H. speciosa, pois as proteínas presentes no látex são as principais responsáveis ​​pela alergia. Este biomaterial pode ser utilizado como fonte não alergênica para desenvolvimento de novos medicamentos.


Subject(s)
Humans , Apocynaceae , Hevea , Wound Healing , Biocompatible Materials , Allergens , Latex
2.
São Paulo; s.n; 20220601. 117 p.
Thesis in Portuguese | LILACS, BBO | ID: biblio-1370713

ABSTRACT

Com a perda de um dente após uma exodontia, tem-se início o processo de remodelação óssea de uma cicatrização espontânea que levará a perdas dimensionais desse alvéolo, principalmente em volume, comprometendo o formato original do rebordo. Técnicas de preservação do rebordo alveolar têm sido descritas há muito tempo, porém, sem um consenso sobre qual procedimento escolher e tampouco qual substituto usar. O objetivo desse estudo foi comparar algumas técnicas já citadas na literatura, utilizando substitutos ósseos e membranas para avaliar as alterações dimensionais horizontais. Foram tratados 25 pacientes (n=5) com necessidade de exodontia de dentes da maxila, com exceção dos molares. Os pacientes foram dispostos aleatoriamente em 5 grupos: GI membrana de politetrafluoretileno denso (Cytoplast®); GII enxerto ósseo xenógeno com uma membrana de colágeno (Bio Oss Collagen® associado a Bio Gide®); GIII membrana de polipropileno (Bone Heal®) 7 dias; GIV membrana de polipropileno (Bone Heal® 21 dias e GV Controle (cicatrização espontânea). O desfecho primário foi a alteração dimensional horizontal do rebordo alveolar encontrada entre as imagens tomográficas de início (T0) antes da exodontia e final (T1) após 6 meses da intervenção, na espessura abaixo da crista óssea em 1 mm (H1), 3 mm (H3) e 5 mm (H5), além da redução em volume do tecido mole avaliadas por meio de modelos de gesso escaneados e sobrepostos com um software de precisão (GOM Inspect®), medidos em 6 pontos em mimímetros a partir da margem gengival (0, 1, 2, 3, 4 e 5). As médias em mm das reduções em espessura óssea foram: em H1 (GI = 1,30, GII = 1,89, GIII = 1,30, GIV = 1,20, GV = 1,97); em H3 (GI = 1,71, GII = 1,98, GIII = 1,16, GIV = 0,34, GV = 1,75) e em H5 (GI = 1,11, GII = 0,74, GIII = 0,86, GIV = 0,26, GV = 1,36) chegando a uma conclusão estatisticamente significante entre os grupos de que GIV apresenta menores desvios que GII. Já as médias em mm das reduções horizontais dos tecidos ósseos foram: Ponto 0 (3,21; 2,26; 1,71; 1,49; 2,57 mm), ponto 1 (2,24; 2,39; 1,58; 1,59; 2,42 mm). Ponto 2 (1,77; 2,20; 1,21; 1,37;2,26 mm), ponto 3 (1,61; 1,90; 1,13; 1,13; 2,03 mm), ponto 4 (1,49; 1,68; 1,21; 0,94; 1,86 mm) e ponto 5 (1,28; 1,69; 1,06; 1,27; 1,70mm) respectivamente para os grupos GI, GII, GII, GIV e GV. Concluindo que houve diferenças entre os grupos estatisticamente significantes entre GIII com menores reduções que GII e GV, GIV com menores reduções que GV. Demonstrando que a técnica de preservação do rebordo alveolar pode ser utilizada como uma alternativa na limitação das perdas horizontais em tecido mole e ósseos.


Subject(s)
Biocompatible Materials
3.
Rev. Odontol. Araçatuba (Online) ; 43(1): 18-23, jan.-abr. 2022. ilus
Article in Portuguese | LILACS, BBO | ID: biblio-1361569

ABSTRACT

O presente artigo tem como objetivo relatar um caso clínico de selamento de perfuração com uma técnica de inserção modificada do MTA. Após a anestesia, foi realizado o isolamento absoluto do dente e remoção do material presente na câmara, com o auxílio do inserto ultrassônico Smart X 1. Realizada a limpeza da perfuração utilizando hipoclorito de sódio e modelagem do canal com o instrumento rotatório, a obturação foi concebida com cone de guta percha e cimento AHPlus. Com broca Gates Glidden #3 foi removida a guta percha até o nível da perfuração. Em seguida, 5 mg de MTA branco foi manipulado, de acordo com recomendações do fabricante usando água destilada na proporção 1:1 e inserido na canaleta de uma régua endodôntica. Com o auxílio do instrumento de RHEM o material foi removido da canaleta e inserido na perfuração, concluindo o vedamento da mesma. Conclui-se neste caso clínico que o selamento de perfuração com MTA obteve sucesso clínico e radiográfico, utilizando a técnica de inserção com MTA modificada(AU)


This article aims to report a clinical case of sealing drilling rig with a modified MTA insertion technique. After anesthesia, the absolute isolation of the tooth and removing the material present in the Chamber, with the aid of ultrasonic Insert Smart X 1. Held drilling cleaning using sodium hypochlorite and modeling the canall with the Rotary instrument, the filling was designed with cone of gutta percha and cement AHPlus. With Gates Glidden bur #3 removed the gutta percha drilling level. Then 5 mg of white MTA was handled, according to manufacturer 's recommendations using distilled water in the ratio 1:1 and inserted in a canal endodontic ruler. With the aid of RHEM instrument the material was removed from the canal and inserted in drilling, completing the enclosure. It is concluded in this case that the sealing of drilling with clinical and radiographic success MTA, using the technique of does not avoid inserting rotating instrument. The obturation was performed with gutta percha cone and AHPlus cement. Removed gutta percha with Gates Glidden drill to drill level. Then, 5 mg of white MTA was handled, according to manufacturer 's recommendations using distilled water in the ratio 1: 1. Soon after, it was inserted in the caneleta of an endodontic ruler and with the aid of the instrument of RHEM the material was inserted in the indicated place. It is concluded in this clinical case that the perforation sealing with MTA obtained clinical and radiographic success, using the insertion technique with modified MTA(AU)


Subject(s)
Humans , Male , Middle Aged , Root Canal Filling Materials , Root Canal Obturation , Root Canal Therapy , Sodium Hypochlorite , Biocompatible Materials , Root Canal Preparation , Gutta-Percha
4.
J. venom. anim. toxins incl. trop. dis ; 28: e20210056, 2022. tab, graf, ilus
Article in English | LILACS, VETINDEX | ID: biblio-1360567

ABSTRACT

The association of scaffolds to repair extensive bone defects can contribute to their evolution and morphophysiological recomposition. The incorporation of particulate biomaterials into three-dimensional fibrin bioproducts together with photobiomodulation therapy (PBM) has potential and can improve regenerative medicine procedures. The objective of this experiment was to evaluate the effects of PBM therapy on critical size defects filled with xenogenic bone substitute associated with fibrin biopolymer. Methods: A critical defect of 8 mm was performed in 36 Wistar male adult rats that were divided into four groups. Groups BC and BC-PBM were defined as controls with defects filled by a clot (without or with PBM, respectively) and groups XS and XS-PBM that comprised those filled with biocomplex Bio-OssTM in association with fibrin biopolymer. PBM was applied immediately after the surgery and three times a week every other day, with the parameters: wavelength of 830 nm, energy density 6.2 J/cm2, output power 30 mW, beam area of 0.116 cm2, irradiance 0.258,62 W/cm2, energy/point 0.72 J, total energy 2.88 J. Fourteen and 42 days after the surgery, animals were euthanatized and subjected to microtomography, qualitative and quantitative histological analysis. Results: The BC-PBM and XS-PBM groups had a similar evolution in the tissue repair process, with a higher density of the volume of new formed bone in relation to the groups without PBM (p = 0.04086; p = 0.07093, respectively). Intense vascular proliferation and bone deposition around the biomaterial particles were observed in the animals of the groups in which biocomplex was applied (XS and XS-PBM). Conclusion: PBM therapy allowed an improvement in the formation of new bone, with a more organized deposition of collagen fibers in the defect area. Biocomplex favored the insertion and permanence of the particulate material in bone defects, creating a favorable microenvironment for accelerate repair process.(AU)


Subject(s)
Biocompatible Materials , Biopolymers , Collagen , Low-Level Light Therapy
5.
Article in English | WPRIM | ID: wpr-929039

ABSTRACT

Carbon nanotube (CNT) composite materials are very attractive for use in neural tissue engineering and biosensor coatings. CNT scaffolds are excellent mimics of extracellular matrix due to their hydrophilicity, viscosity, and biocompatibility. CNTs can also impart conductivity to other insulating materials, improve mechanical stability, guide neuronal cell behavior, and trigger axon regeneration. The performance of chitosan (CS)/polyethylene glycol (PEG) composite scaffolds could be optimized by introducing multi-walled CNTs (MWCNTs). CS/PEG/CNT composite scaffolds with CNT content of 1%, 3%, and 5% (1%=0.01 g/mL) were prepared by freeze-drying. Their physical and chemical properties and biocompatibility were evaluated. Scanning electron microscopy (SEM) showed that the composite scaffolds had a highly connected porous structure. Transmission electron microscope (TEM) and Raman spectroscopy proved that the CNTs were well dispersed in the CS/PEG matrix and combined with the CS/PEG nanofiber bundles. MWCNTs enhanced the elastic modulus of the scaffold. The porosity of the scaffolds ranged from 83% to 96%. They reached a stable water swelling state within 24 h, and swelling decreased with increasing MWCNT concentration. The electrical conductivity and cell adhesion rate of the scaffolds increased with increasing MWCNT content. Immunofluorescence showed that rat pheochromocytoma (PC12) cells grown in the scaffolds had characteristics similar to nerve cells. We measured changes in the expression of nerve cell markers by quantitative real-time polymerase chain reaction (qRT-PCR), and found that PC12 cells cultured in the scaffolds expressed growth-associated protein 43 (GAP43), nerve growth factor receptor (NGFR), and class III β‍-tubulin (TUBB3) proteins. Preliminary research showed that the prepared CS/PEG/CNT scaffold has good biocompatibility and can be further applied to neural tissue engineering research.


Subject(s)
Animals , Axons , Biocompatible Materials/chemistry , Chitosan/chemistry , Nanotubes, Carbon/chemistry , Nerve Regeneration , Polyethylene Glycols , Porosity , Rats , Tissue Engineering/methods , Tissue Scaffolds/chemistry
6.
Rev. odontol. UNESP (Online) ; 51: e20220005, 2022. ilus
Article in English | LILACS, BBO | ID: biblio-1377171

ABSTRACT

Introduction: Some experimental models have been used to evaluate the use of biomaterials in bone regeneration. Among them are the critical size defects (CSD) created in rat calvaria. An experimental model has been described in the literature, in which "L" markings are performed on the margins of the bone defects in order to assist in the precise identification of these defects during laboratory processing and analysis of the results. In the proposed model, the "L" markings are filled with amalgam. Objective: The purpose of the present study was to evaluate the amalgam replacement of an experimental bony defect model in rat calvaria by heated or unheated glass ionomer. Material and method: 24 rats were used. A 5 mm CSD was created at each animal calvaria. Two "L" shaped markings were made 2 mm from the margins of the bone defect, filled with amalgam (Group AM), heated glass ionomer cement (Group GIh) or not (Group GI). The animals were euthanized 15 days postoperatively. The areas of the surgical defect and the L-shaped marking were histomorphometrically analyzed and the data were analyzed statistically (p <0.05). Result: There were no significant clinical, histological or methodological differences among the experimental groups. Conclusion: It can be concluded that GI can replace AM in the proposed experimental model and GI heating did not promote additional benefits.


Introdução: Alguns modelos experimentais têm sido usados para avaliar o uso de biomateriais na regeneração óssea. Entre eles estão os defeitos de tamanho crítico (DTC) criados em calvárias de ratos. Um modelo experimental foi descrito na literatura onde marcações em L são realizadas nas margens do defeito ósseo para auxiliar na identificação precisa desses defeitos durante o processamento laboratorial e análise dos resultados. No modelo experimental proposto, as marcações em "L" são preenchidas com amálgama. Objetivo: Avaliar a substituição do amálgama por ionômero de vidro aquecido ou não em um modelo experimental para identificação de defeito ósseo criado em calvária de ratos. Material e método: Foram utilizados 24 ratos. Um DTC de 5 mm de diâmetro foi criado na calvária de cada animal. Duas marcações em "L" foram realizadas a 2 mm das margens do defeito ósseo, preenchidas com amálgama (Grupo AM), ionômero de vidro aquecido (Grupo CIVaq) ou não (Grupo CIV). Os animais foram eutanasiados aos 15 dias pós-operatórios. A área do defeito cirúrgico e das marcações em "L" foram histomorfometricamente avaliadas e os dados estatisticamente analisados (p<0,05). Resultado: Não houve diferença estatisticamente significativa entre os grupos experimentais para as análises metodológicas, clínicas ou histomorfométrica realizadas. Conclusão: Dentro dos limites deste estudo, pode-se concluir que CIV pode substituir o AM no modelo experimental proposto e o aquecimento do CIV não promoveu benefícios adicionais.


Subject(s)
Rats , Skull , Biocompatible Materials , Bone Regeneration , Dental Amalgam , Glass Ionomer Cements , Mathematical Computing , Analysis of Variance
7.
Braz. dent. sci ; 25(2): 1-8, 2022. ilus, tab
Article in English | LILACS, BBO | ID: biblio-1368244

ABSTRACT

Increasing prevalence of erosive tooth wear (ETW) is notable mainly due to current knowledge and its early diagnosis. Once teeth are functionally and/or esthetically affected, dental restorations may become necessary. Materials capable of interacting with the eroded dental tissue allied to their resistance to subsequent continuous erosive challenges are desirable. Giomer technology based on S-PRG (surface pre-reactive glass) filler has been launching to provide benefits for the dental treatment due to its innovative multi-ionic release system, which involves fluoride. This case report describes the employment of preventive and restorative materials based on this technology for a patient under frequent erosive challenges and complaining about dental sensitivity. Patient reported immediate reduction on this sensitivity and the restorations has been followed up for 2 years, presenting satisfactory performance. S-PRG-based systems seem to be promissory for preventive and therapeutic management of ETW used simultaneously with the patient compliance. (AU)


O aumento da prevalência do desgaste dentário erosivo (DDE) é notável especialmente devido ao conhecimento atual e seu diagnóstico precoce. Uma vez que os dentes são afetados funcional e/ou esteticamente, restaurações dentárias se tornam necessárias. Materiais focados na habilidade de interagir com o tecido dentário erodido associados com sua resistência aos desafios erosivos contínuos subsequentes são desejáveis. A tecnologia Giomer baseada em partículas S-PRG (superfície de vidro pré-reativo) foi lançada no mercado para oferecer benefícios para o tratamento odontológico devido ao seu sistema inovador de liberação multi-iônica, que envolve o flúor. Esse caso clínico descreve o uso de materiais preventivo e restaurador baseados nessa tecnologia em um paciente em desafio erosivo e com queixa de sensibilidade. O paciente relatou redução imediata da sensibilidade e as restaurações foram acompanhadas por 2 anos apresentando desempenho satisfatório. Sistemas baseados em S-PRG se mostram promissores para manejo preventivo e terapêutico do DDE empregados simultaneamente à colaboração do paciente.(AU)


Subject(s)
Humans , Male , Adult , Tooth Erosion , Biocompatible Materials , Stimuli Responsive Polymers , Inlays
8.
Rev. Odontol. Araçatuba (Impr.) ; 42(3): 25-31, set.-dez. 2021. ilus
Article in Portuguese | LILACS, BBO | ID: biblio-1284114

ABSTRACT

Os pilares personalizados são usados em reabilitações protéticas sobre implantes quando há exigências de angulação, perfil de emergência e margem gengival, em que os pilares convencionais não conseguem reabilitar de forma correta os conceitos de forma, função e estética. Através de um relato de caso clínico, onde houve a reabilitação dos elementos 21 e 22, com o uso de implante e próteses, usando o pilar personalizado em zircônia sobre o implante do 22, confeccionado com a tecnologia CAD/CAM. Esse sistema de pilares personalizados oferecem o desenho mais próximo do ideal e o ajuste mais perfeito em relação ao implante e ao tecido gengival, além de considerar as vantagens e desvantagen em relação aos pilares convencionais pré-fabricados nas questões de tempo de tratamento, custos e resultado estético e biológico. Concluimos que a reabilitação com pilares personalizados CAD/CAM confere uma excelente alternativa reabilitadora, devolvendo função e estética aos pacientes, desde que haja conhecimento adequado por parte dos profissionais envolvidos(AU)


Custom abutments are used in prosthetic rehabilitation on implants when angulation, emergence profile and gingival margin are used, where abutments are not recovered in a correct way to rehabilitate the concepts of shape, functions and aesthetics. Through a clinical case, where the elements 21 and 22 were rehabilitated, with the use of implants and practices, using the personalized abutments in zirconia, made with CAD / CAM technology. This customized system and abutments offers the closest ideal design and the most perfect fit in relation to the implant and gingival tissue, in addition to considering advantages and disadvantages in relation to the prefabricated applied abutments in terms of treatment time, costs and results aesthetic and biological. Conclude that a rehabilitation with customized CAD / CAM abutments provides an excellent rehabilitation alternative, returning function and aesthetics to patients, as long as there is adequate knowledge for part of the professionals involved(AU)


Subject(s)
Humans , Female , Aged , Dental Implants , Computer-Aided Design , Biocompatible Materials , Dental Prosthesis, Implant-Supported , Esthetics, Dental
9.
Rev. ADM ; 78(5): 291-296, sept.-oct. 2021. ilus
Article in Spanish | LILACS | ID: biblio-1348330

ABSTRACT

La anquilosis de la articulación temporomandibular en niños continúa siendo una patología presente a pesar de los avances médicos y sociales. El tratamiento de esta patología en niños tiene como objetivos restablecer la apertura bucal y mejorar la estética facial cuando se presentan hipoplasias o micrognatias asociadas. El uso de materiales aloplásticos para el tratamiento de la anquilosis temporomandibular en niños es evitar la reanquilosis y disminuir riesgos, molestias y costos que ocasionan la toma y aplicación de injertos, siendo utilizados con buenos resultados en niños en otras especialidades como la Traumatología y Ortopedia. Estos procedimientos pueden llevarse a cabo de manera segura y predecible. En este artículo se reportan dos casos de anquilosis temporomandibular en niños, tratados con materiales aloplásticos, llevados a cabo en la Unidad Médica de Alta Especialidad No. 71 del Instituto Mexicano del Seguro Social, Torreón, Coahuila, México, con un seguimiento de 11 y 16 años de postoperatorio, demostrando que se trata de una buena opción de tratamiento sin presentar alteraciones al crecimiento y desarrollo de los pacientes (AU)


Temporomandibular ankilosis in children is pathology still present despite the medical and social advances. The treatment of this pathology in children aims to restore mouth opening and improve facial aesthetics when hypoplasia or micrognatia are present. The use of alloplastic materials to treat temporomandibular ankilosis in children is to prevent the re ankilosis and reduce discomfort, risks, and cost causing by the take and application of graft, alloplastic materials being used with good results in children in other specialties such as Traumatology and Orthopedics. These procedures can be made safely and predictably. This article describes two cases of temporomandibular ankilosis in children, treated with alloplastic materials, carried out in the Medical Unit of High Specialty, number 71, of the Mexican Institute Social Security, Torreon, Coahuila, Mexico, with follow up of cases 11 and 16 years of postoperative, prove that is a good option of treatment, without presenting any alterations in growth and development of patients (AU)


Subject(s)
Humans , Male , Child , Biocompatible Materials , Temporomandibular Joint Disorders/therapy , Ankylosis/therapy , Maxillofacial Prosthesis , Titanium , Follow-Up Studies , Chromium Alloys , Genioplasty , Mandibular Condyle/injuries
10.
Rev. Odontol. Araçatuba (Impr.) ; 42(2): 52-55, maio-ago. 2021. ilus
Article in Portuguese | LILACS, BBO | ID: biblio-1283891

ABSTRACT

Pacientes com históricos de defeitos ósseos provocados por infecções, malformação congênita, neoplasias, deformação por iatrogenia, radioterapia e trauma buscam contornos faciais mais harmônicos através da reabilitação cirúrgica. Para facilitar a reconstrução maxilofacial dois grupos de materiais podem ser utilizados, os enxertos ósseos e os materiais aloplásticos. O objetivo é relatar um caso incomum de infecção e exposição de material utilizado para enxertia a base de polimetilmetacrilato, bem como a sua posterior reabordagem cirúrgica. Embora o Polimetilmetacrilato aparente ser seguro, ele exibe complicações diversas em função da imunologia do hospedeiro, que poderá reagir de diferentes formas. Desse modo, faz-se necessário ressaltar a importância da prevenção e proservação de cada caso de forma individualizada(AU)


Patients with a history of bone defects caused by infections, congenital malformation, neoplasms, iatrogenic deformation, radiotherapy and trauma seek more harmonious facial contours through surgical rehabilitation. To facilitate maxillofacial reconstruction, two groups of materials can be used, bone grafts and alloplastic materials. The objective is to report an unusual case of infection and exposure of material used for grafting with polymethylmethacrylate, as well as its subsequent surgical approach. Although Polymethylmethacrylate appears to be safe, it exhibits different complications depending on the host's immunology, which may react in different ways. Thus, it is necessary to emphasize the importance of preventing and preserving each case individually(AU)


Subject(s)
Humans , Female , Middle Aged , Bone Transplantation , Polymethyl Methacrylate , Maxillofacial Injuries , Orbit , Surgery, Oral , Zygoma , Biocompatible Materials , Iatrogenic Disease , Maxilla
11.
Rev. ADM ; 78(4): 195-204, jul.-ago. 2021. ilus, tab
Article in Spanish | LILACS | ID: biblio-1292500

ABSTRACT

Desde los sesentas, con la invención del vidrio bioactivo, los tratamientos de remineralización se han popularizado entre los cirujanos dentistas y su utilización es cada vez mayor; la remineralización, en conjunto con las adecuadas medidas de higiene preventiva, representa uno de los mejores abordajes mínimamente invasivos y a un costo comparativamente bajo. Este estudio documental tiene por objetivo establecer una mejor comprensión del uso clínico de los biomateriales que inducen la remineralización de la superficie del esmalte dental y dentina. Se realizó una exploración utilizando motores de búsqueda (bases de datos en PubMed, Medigraphic, y Science Direct). El proceso de localización de los estudios relevantes se efectuó introduciendo palabras clave como: silicatos de calcio, fosfopéptidos de caseína-fosfato de calcio amorfo, remineralización, esmalte y dentina, incluyéndose en el procedimiento artículos de antigüedad no superior a siete años, en español e inglés, publicados en revistas científicas aprobadas por pares.Actualmente, no es posible remineralizar del todo la estructura dentaria, por lo cual, en un futuro cercano, los esfuerzos de la odontología de remineralización deben apuntar al desarrollo de agentes biomiméticos inteligentes que restauren al cien por ciento la estructura dental perdida (AU)


Since the sixties, with the invention of bioactive glass, remineralization treatments have become popular among dental surgeons. Their usage is increasing; remineralization, in conjunction with appropriate preventive hygiene measures, represents one of the best minimally invasive treatments at a relatively low cost. This documentary study aims to establish a better understanding of the clinical use of biomaterials that induce remineralization of the surface of teeth enamel and dentin. A search was conducted using search engines (PubMed and Medigraphic databases, and Science Direct). The search process for the relevant studies was carried out by introducing keywords such as calcium silicates, phosphopeptides of amorphous calcium casein-phosphate, remineralization, enamel and dentin, including in the search articles no older than seven years in Spanish and English published in scientific reviewed journals. Currently, it is not possible to completely remineralize the dentary structure so, in the near future, remineralization dentistry efforts should aim to develop (AU)


Subject(s)
Humans , Tooth Remineralization/instrumentation , Biocompatible Materials , Dental Enamel/drug effects , Dentin/drug effects , Phosphopeptides/therapeutic use , Caseins , Calcarea Silicata/therapeutic use
12.
Rev. cuba. invest. bioméd ; 40(2): e570, 2021. graf
Article in Spanish | LILACS, CUMED | ID: biblio-1347461

ABSTRACT

Introducción: El panorama demográfico en el mundo está cambiando. La población mayor de 60 años es el segmento que está creciendo más rápidamente y en el que las enfermedades del tejido óseo se presentan con más frecuencia, lo que aumenta la demanda de materiales y tecnologías apropiadas para restaurar estos tejidos. Objetivo: Analizar la información que se ha generado sobre el desarrollo de biomateriales compuestos para la reparación ósea, con énfasis en la identificación de las tecnologías emergentes basadas en el uso del campo electromagnético, sus aplicaciones y potencialidades. Métodos: Se consultaron trabajos científicos publicados en libros, revistas, patentes y tesis. El 80 por ciento de la documentación seleccionada pertenece al periodo 2010-2019. Análisis e integración de la información: Los métodos identificados fueron clasificados en cinco grupos: electrodeposición química, ya sea por electrólisis, electroforesis o síntesis electroforética in situ; electroporación; electrohilado; control magnético distal y bioestimulación electromagnética de células y tejidos, directamente o por la introducción de dispositivos que convierten la energía electromagnética en energía mecánica. Conclusiones: Estos métodos permiten la conformación de matrices celulares y acelulares compuestas y, además, dispositivos bioestimuladores con control de los parámetros de construcción y acción, de tal manera, que se logran procesos con mayor grado de reproducibilidad y a la medida de los requerimientos específicos para cada paciente(AU)


Introduction: The global demographic panorama is changing. The population aged over 60 years is the fastest growing segment, as well as the one where bone tissue diseases are most common, increasing the demand of appropriate materials and technologies to restore those tissues. Objective: To analyze the information so far generated about the development of composite biomaterials for bone repair, with an emphasis on the identification of emerging technologies based on the use of the electromagnetic field, its applications and potential. Methods: An analysis was performed of scientific papers published in books, journals, patents and theses. Of the documentation selected, 80 percent was from the period 2010-2019. Data analysis and integration: The methods identified were classified into five groups: chemical electrodeposition, be it by in situ electrophoretic synthesis, electrolysis or electrophoresis; electroporation; electrospinning; distal magnetic control and electromagnetic biostimulation of cells and tissues, either directly or incorporating devices which convert electromagnetic energy into mechanical energy. Conclusions: These methods permit the conformation of composite cellular and acellular matrices as well as biostimulator devices controlling construction and action parameters in such a way that the processes obtained display greater reproducibility and are more in keeping with the specific requirements of each patient(AU)


Subject(s)
Humans , Biocompatible Materials/analysis , Electric Stimulation/methods , Electromagnetic Fields
13.
Arq. Ciênc. Vet. Zool. UNIPAR (Online) ; 24(1, cont.): e2403, jan-jun. 2021. tab, graf
Article in Portuguese | LILACS, VETINDEX | ID: biblio-1252764

ABSTRACT

O Whatman FTA-Card® é um papel-filtro quimicamente tratado, destinado à coleta, transporte, armazenamento de amostras para posterior extração de ácidos nucléicos. A tecnologia FTA-Card® é utilizada para manter estável DNA e RNA em temperatura ambiente, podendo ser utilizados para fixação de uma ampla variedade de material orgânico ou tecidos. Foram realizados testes para certificar sua eficiência na conservação do material a ser analisado com o intuito de eliminar a cadeia fria de conservação, agilizando o processo e diminuindo os custos da execução de exames moleculares associados ao diagnóstico de patologias. Foram testadas oito amostras de felinos na forma de sangue total e soro, para a extração utilizou-se o kit Magazorb RNA Total mini-prep kit (Promega®, EUA), para o diagnóstico foi utilizada a técnica de PCR em tempo real para amplificar o gene CI2 de mamíferos, a fim de visualizar a eficácia na conservação de ácidos nucleicos. A utilização desse método torna possível que o material biológico seja enviado por serviços de transporte postais, reduzindo os custos e viabilizando diagnósticos provenientes de áreas mais remotas.(AU)


Whatman FTA-Card® is a chemically treated filter paper intended for the collection, transport, and storage of samples for later extraction of nucleic acids. FTA-Card® technology is used to keep DNA and RNA stable at room temperature and can be used to fix a wide variety of organic material or tissues. Tests were carried out to certify its efficiency in the conservation of the material to be analyzed in order to eliminate the cold conservation chain, speeding up the process and decreasing the costs of performing molecular tests associated with the diagnosis of pathologies. By using this method, biological material can be sent by postal transport services, reducing costs and making diagnoses from more remote areas feasible. Samples of feline specimens were tested in the form of whole blood and serum, using the Magazorb RNA Total mini-prep kit (Promega®, USA) for the extraction. Diagnosis was performed using real-time PCR technique to amplify the mammalian CI2 gene in order to visualize the effectiveness in conserving nucleic acids.(AU)


Whatman FTA-Card® es un papel de filtro tratado químicamente, destinado a la recogida, transporte, almacenamiento de muestras para su posterior extracción de ácidos nucleicos. La tecnología FTA-Card® se usa para mantener el ADN y el ARN estables a la temperatura ambiente y se puede usar para la fijación de una amplia variedad de materiales o tejidos orgánicos. Se realizaron pruebas para certificar su eficiencia en la conservación del material a analizar con el fin de eliminar la cadena de frío de conservación, agilizando el proceso y reduciendo los costos de realización de pruebas moleculares asociadas al diagnóstico de patologías. Se analizaron ocho muestras felinas en forma de sangre total y suero, para la extracción se utilizó el mini-prep kit Magazorb RNA Total (Promega®, USA), para el diagnóstico se utilizó la técnica de PCR en tiempo real para amplificar el CI2 de mamífero gen, con el fin de visualizar la efectividad en la conservación de ácidos nucleicos. El uso de ese método permite el envío de material biológico por los servicios de transporte postal, lo que reduce los costes y permite realizar diagnósticos desde zonas más remotas.(AU)


Subject(s)
Biocompatible Materials , DNA , Real-Time Polymerase Chain Reaction
14.
Medisan ; 25(2)mar.-abr. 2021.
Article in Spanish | LILACS, CUMED | ID: biblio-1250353

ABSTRACT

La endodoncia regenerativa comprende un conjunto de procedimientos biológicos que se efectúan en los dientes permanentes con ápice no formado y necrosis pulpar, cuyo fin es sustituir los tejidos dañados, incluidas la dentina y la estructura radicular, así como las células del complejo pulpodentinario. En este artículo se describen las bases celulares y moleculares de esta terapia, sustentada en una compleja interacción entre las células madre dentales de la papila apical, los factores de crecimiento y los biomateriales con el microambiente donde se va a restablecer. Asimismo, se argumenta cómo las condiciones ambientales en las que se lleva a cabo el proceso terapéutico influyen en la regeneración, con una función esencial en la regulación de la diferenciación de los tejidos.


The regenerative endodontics comprises a group of biological procedures that are made in the permanent teeth with non-formed apex and pulpar necrosis whose end is to substitute the damaged tissues, including the dentine and radicular structure, as well as the cells of the dentin pulp complex. The cellular and molecular basis of this therapy are described in this work, sustained in a complex interaction between the dental stem cells of the apical papilla, the growth factors and biomaterials with the microenvironment where it will be recover. Likewise, it was argued how the environmental conditions in which the therapeutic process is carried out influence in the regeneration, with an essential function in the regulation of the differentiation of tissues.


Subject(s)
Stem Cells , Dentition, Permanent , Regenerative Endodontics/methods , Biocompatible Materials
15.
Braz. j. biol ; 81(1): 53-61, Feb. 2021. tab, graf
Article in English | LILACS | ID: biblio-1153329

ABSTRACT

Abstract The aim of this study was to analyze the biological behavior and osteogenic potential of magnesium (Mg) substituted hydroxyapatite (HA) microspheres, implanted in a critical bone defect, considering that this ion is of great clinical interest, since it is closely associated with homeostasis and bone mineralization. For the purpose of this study, 30 rats were used to compose three experimental groups: GI - bone defect filled with HA microspheres; GII - bone defect filled with HA microspheres replaced with Mg; GIII - empty bone defect; evaluated at biological points of 15 and 45 days. The histological results, at 15 days, showed, in all the groups, a discrete chronic inflammatory infiltrate; biomaterials intact and surrounded by connective tissue; and bone neoformation restricted to the borders. At 45 days, in the GI and GII groups, an inflammatory response of discrete granulomatous chronic type was observed, and in the GIII there was a scarce presence of mononuclear inflammatory cells; in GI and GII, the microspheres were seen to be either intact or fragmented, surrounded by fibrous connective tissue rich in blood vessels; and discrete bone neoformation near the edges and surrounding some microspheres. In GIII, the mineralization was limited to the borders and the remaining area was filled by fibrous connective tissue. It was concluded that the biomaterials were biocompatible and osteoconductive, and the percentage of Mg used as replacement ion in the HA did not favor a greater bone neoformation in relation to the HA without the metal.


Resumo O objetivo deste estudo foi analisar o comportamento biológico de microesferas de hidroxiapatita (HA) substituída com magnésio (Mg) durante o reparo de defeito ósseo crítico, tendo em vista que este íon é de grande interesse clínico, pois está intimamente associado à homeostasia e à mineralização óssea. Para tanto, utilizou-se 30 ratos para compor três grupos experimentais: GI - defeito ósseo preenchido com microesferas de HA; GII - defeito ósseo preenchido com microesferas de HA substituída com Mg; GIII (controle) - defeito ósseo vazio; avaliados nos pontos biológicos de 15 e 45 dias. Os resultados histológicos evidenciaram, aos 15 dias, discreto infiltrado inflamatório crônico e neoformação óssea restrita às bordas, em todos os grupos. Nos grupos GI e GII, os biomateriais mantiveram-se íntegros e circundados por tecido conjuntivo frouxo. Aos 45 dias, notou-se resposta inflamatória do tipo crônica granulomatosa discreta nos grupos GI e GII, e no GIII presença escassa de células inflamatórias mononucleares. As microesferas implantadas no GI e GII mantiveram-se, em sua maioria, íntegras e envolvidas por tecido conjuntivo fibroso. Notou-se discreta neoformação óssea próxima às bordas e circunjacente a algumas microesferas. No GIII, a mineralização limitou-se às bordas e a área remanescente foi preenchida por tecido conjuntivo fibroso. Conclui-se que os biomateriais foram biocompatíveis, bioativos, osteocondutores e apresentaram biodegradação lenta, indicando seu grande potencial para em aplicações clínica como biomaterial de preenchimento.


Subject(s)
Animals , Rats , Durapatite , Magnesium , Osteogenesis , Biocompatible Materials , Bone Regeneration
16.
Rev. bras. ciênc. vet ; 28(1): 23-29, jan./mar. 2021. il.
Article in Portuguese | LILACS, VETINDEX | ID: biblio-1368354

ABSTRACT

Na procura por material alternativo no tratamento de feridas cutâneas, buscou-se com este estudo avaliar o comportamento da túnica vaginal canina conservada em glicerina a 98% como curativo biológico no tratamento de feridas cutâneas provocadas experimentalmente em ratos. Foram utilizados 16 ratos Wistar, nos quais foram induzidas experimentalmente duas lesões cutâneas na região dorsal, sendo uma cranial e a outra caudal. Uma ferida serviu de controle, a qual não recebeu tratamento, a outra recebeu a túnica vaginal recobrindo toda extensão da lesão. A seleção de qual das feridas, cranial ou caudal, iria receber a membrana foi feita de forma aleatória. Os animais foram distribuídos em quatro grupos de igual número para análises clínicas, macroscópicas e histológicas decorridos três, sete, 14 e 21 dias de pós-operatório. Na avaliação clínica e macroscópica pode-se observar que o processo de reparação teve evolução semelhante tanto no grupo controle quanto no grupo membrana. Na análise histopatológica foi constatada grande quantidade de infiltrado inflamatório nos períodos iniciais, no entanto, decorridos 21 dias, a epiderme já se encontrava reparada, e em todos os momentos foi observada maior presença de tecido conjuntivo no grupo membrana, sugerindo maior precocidade na cicatrização. Deste modo, concluiu-se que a túnica vaginal canina pode ser utilizada como nova alternativa no tratamento de feridas cutâneas.


In the search for alternative material in the treatment of cutaneous wounds, this study aimed to evaluate the behavior of canine vaginal tunic conserved in glycerin 98% as a biological dressing in the treatment of cutaneous wounds provoked experimentally in rats. Sixteen animals were used in which two cutaneous lesions were caused in the dorsal region, one cranial and the other caudal. One wound served as a control which did not receive treatment, the other received the vaginal tunic covering all extension of the lesion. The selection of which of the wounds, cranial or caudal, would receive the membrane was made at random. The animals were divided into four groups of equal numbers for clinical, macroscopic, and histological analyzes after three, seven, 14 and 21 postoperative days. In the clinical and macroscopic evaluation, it can be observed that the healing process had similar evolution in both the control group and the membrane group. In the histopathological analysis, a large amount of inflammatory infiltrate was observed in the initial periods; however, after 21 days the epidermis was already healed, and at all times a greater presence of connective tissue was observed in the membrane group, suggesting a greater precocity in healing. Thus, it was concluded that the canine vaginal tunic can be used as a new alternative in the treatment of cutaneous wounds.


Subject(s)
Animals , Rats , Wound Healing/physiology , Biological Dressings/veterinary , Rats, Wistar/injuries , Wounds and Injuries/therapy , Biocompatible Materials/therapeutic use
17.
Braz. J. Vet. Res. Anim. Sci. (Online) ; 58: e179885, 2021. ilus, graf
Article in English | LILACS, VETINDEX | ID: biblio-1347989

ABSTRACT

Hydroxyapatite, chitosan, and carbon nanotube composite biomaterial were developed to improve bone healing. Previous studies suggested that a combination of biomaterials and mesenchymal stem cells (MSCs) can potentially help promote bone regeneration. In the present study, we first developed hydroxyapatite, chitosan, and carbon nanotube composite biomaterial. Then, the effect of different concentrations of the extract on the viability of Vero cells (ATCC CCL-81) and MSCs obtained from sheep bone marrow using methylthiazol tetrazolium (MTT) and propidium iodide (PI) assays were evaluated. The biomaterial group demonstrated an absence of cytotoxicity, similar to the control group. Samples with 50% and 10% biomaterial extract concentrations showed higher cell viability compared to samples from the control group (MTT assay). These results suggest that the presence of this composite biomaterial can be used with MSCs. This study also concluded that hydroxyapatite, chitosan, and carbon nanotube composite biomaterial were not cytotoxic. Therefore, these could be used for performing in vivo tests.(AU)


O compósito à base de hidroxiapatita, quitosana e nanotubo de carbono foi desenvolvido com o intuito de auxiliar na consolidação óssea. Estudos anteriores sugerem que a combinação de substitutos ósseos e células-tronco mesenquimais (CTM) podem auxiliar a potencializar e promover a regeneração óssea. No presente estudo, o biomaterial foi desenvolvido e a viabilidade e a citotoxicidade de células Vero (ATCC CCL-81) e CTM obtidas de medula óssea provenientes de ovinos utilizando ensaios metil-tiazol-tetrazólio, MTT e iodeto de propídeo (PI) foram avaliadas em diferentes concentrações de extrato desse compósito. O compósito demonstrou ausência de citotoxicidade com comportamento semelhante ao grupo controle. Amostras com 50% e 10% de concentração de extrato do compósito mostraram resultados maiores comparados ao grupo controle (ensaio MTT). Esses resultados também sugerem que a presença do biomaterial pode ser utilizada em associação a CTM. Assim, esse estudo conclui que o compósito apresentado de hidroxiapatita, quitosana e nanotubo de cabono não foi considerado citotóxico e pode ser utilizado em teste in vivo.(AU)


Subject(s)
Animals , Biocompatible Materials , Durapatite , Chitosan , Cytotoxicity, Immunologic , Nanotubes, Carbon , Mesenchymal Stem Cells
18.
Article in English | WPRIM | ID: wpr-888699

ABSTRACT

Tissue engineering approaches have emerged recently to circumvent many limitations associated with current clinical practices. This elegant approach utilizes a natural/synthetic biomaterial with optimized physiomechanical properties to serve as a vehicle for delivery of exogenous stem cells and bioactive factors or induce local recruitment of endogenous cells for in situ tissue regeneration. Inspired by the natural microenvironment, biomaterials could act as a biomimetic three-dimensional (3D) structure to help the cells establish their natural interactions. Such a strategy should not only employ a biocompatible biomaterial to induce new tissue formation but also benefit from an easily accessible and abundant source of stem cells with potent tissue regenerative potential. The human teeth and oral cavity harbor various populations of mesenchymal stem cells (MSCs) with self-renewing and multilineage differentiation capabilities. In the current review article, we seek to highlight recent progress and future opportunities in dental MSC-mediated therapeutic strategies for tissue regeneration using two possible approaches, cell transplantation and cell homing. Altogether, this paper develops a general picture of current innovative strategies to employ dental-derived MSCs combined with biomaterials and bioactive factors for regenerating the lost or defective tissues and offers information regarding the available scientific data and possible applications.


Subject(s)
Biocompatible Materials , Cell Differentiation , Humans , Mesenchymal Stem Cell Transplantation , Mesenchymal Stem Cells , Tissue Engineering
19.
Acta cir. bras ; 36(7): e360704, 2021. tab, graf
Article in English | LILACS, VETINDEX | ID: biblio-1339002

ABSTRACT

ABSTRACT Purpose To analyze and compare the reactions at the interface between the composite, composed of fragmented heterologous mineralized bone matrix (MOMHF) and polymethylmethacrylate (PMMA), and the rabbit's tibias, through macroscopic evaluation and scanning electron microscopy (SEM) in different periods. Methods In this study, 12 New Zealand adult rabbits were used (E1: n = 3, E2: n = 3, E3: n = 3 and E4: n = 3). They had the right tibial defects filled with composite and were evaluated immediately after surgery and at 30, 60, 90, and 120 days. Results The composites were incorporated and integrated into the recipient beds in 100% of the cases, defined by the MOMHF osseointegration and the PMMA fibrointegration, with no sign of infection, migration, or rejection. Conclusions The behavior of the composites in the recipient beds demonstrates that these biomaterials have the potential to be used in bone defect repairs, offering, thus, better quality of life to the orthopedic patient.


Subject(s)
Bone Matrix , Polymethyl Methacrylate , Quality of Life , Rabbits , Tibia/surgery , Biocompatible Materials , Osseointegration
20.
Acta cir. bras ; 36(6): e360605, 2021. tab, graf
Article in English | LILACS, VETINDEX | ID: biblio-1284912

ABSTRACT

ABSTRACT Purpose To evaluate and compare two types of different scaffolds in critical bone defects in rats. Methods Seventy male Wistar rats (280 ± 20 grams) divided into three groups: control group (CG), untreated animals; biomaterial group 1 (BG1), animals that received the scaffold implanted hydroxyapatite (HA)/poly(lactic-co-glycolic) acid (PLGA); and biomaterial group 2 (BG2), animals that received the scaffolds HA/PLGA/Bleed. The critical bone defect was induced in the medial region of the skull calotte with the aid of an 8-mm-diameter trephine drill. The biomaterial was implanted in the form of 1.5 mm thick scaffolds, and samples were collected after 15, 30 and 60 days. Non-parametric Mann-Whitney test was used, with the significance level of 5% (p ≤ 0.05). Results Histology revealed morphological and structural differences of the neoformed tissue between the experimental groups. Collagen-1 (Col-1) findings are consistent with the histological ones, in which BG2 presented the highest amount of fibers in its tissue matrix in all evaluated periods. In contrast, the results of receptor activator of nuclear factor kappa-Β ligand (Rank-L) immunoexpression were higher in BG2 in the periods of 30 and 60 days, indicating an increase of the degradation of the biomaterial and the remodeling activity of the bone. Conclusions The properties of the HA/PLGA/Bleed scaffold were superior when compared to the scaffold composed only by HA/PLGA.


Subject(s)
Animals , Male , Rats , Biocompatible Materials , Tissue Scaffolds , Osteogenesis , Bone Regeneration , Rats, Wistar , Polylactic Acid-Polyglycolic Acid Copolymer
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