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1.
Rev. Círc. Argent. Odontol ; 80(231): 6-13, jul. 2022. ilus, tab, graf
Article in Spanish | LILACS | ID: biblio-1391619

ABSTRACT

Este trabajo tuvo como objetivo conocer la fiabilidad de la impresora 3D (i3D) aditiva por Matriz de Proceso Digital de Luz (MDLP) Hellbot modelo Apolo®, a través de verificar la congruencia dimensional entre las mallas de modelos impresos (MMi) y su correspondiente archivo digital de origen (MMo), obtenido del software de planificación ortodontica Orchestrate 3D® (O3D). Para determinar su uso en odontología y sus posibilidades clínicas, fue comparada entre cinco i3D de manufactura aditiva, dos DLP, dos por estereolitografía (SLA) y una por Depósito de Material Fundido (FDM). La elección de las cinco i3D se fundamentó en su valor de mercado, intentando abarcar la mayor diversidad argentina disponible. Veinte modelos fueron impresos con cada i3D y escaneados con Escáner Intraoral (IOS) Carestream modelo 3600® (Cs3600). Las 120 MMi fueron importadas dentro del programa de ingeniería inversa Geomagic® Control X® (Cx) para su análisis 3D, consistiendo en la superposición de MMo con cada una de las MMi. Luego, una evaluación cualitativa de la desviación entre la MMi y MMo fue realizada. Un análisis estadístico cuidadoso fue realizado obteniendo como resultado comparaciones en 3d y 2d. Las coincidencias metrológicas en la superposición tridimensional permitieron un análisis exhaustivo y fácilmente reconocible a través de mapas colorimétricos. En el análisis bidimensional se plantearon planos referenciados dentariamente desde la MMo, para hacer coincidir las mediciones desde el mismo punto de partida dentaria. Los resultados fueron satisfactorios y muy alentadores. Las probabilidades de obtener rangos de variabilidad equivalentes a +/- 50µm fueron de un 40,35 % y de +/- 100µm un 71,04 %. Por lo tanto, te- niendo en cuenta las exigencias de congruencia dimensional clínicas de precisión y exactitud a las cuales es sometida nuestra profesión odontológica, se evitan problemas clínicos arrastrados por los errores dimensionales en la manufactura (Cam) (AU)


The objective of this study was to determine the reliability of the Hellbot Apollo® model additive 3D printer (i3D) by Matrix Digital Light Processing (MDLP) by verifying the dimensional congruence between the printed model meshes (MMi) and their corresponding digital source file (MMo), obtained from the Orchestrate 3D® (O3D) orthodontic planning software. A comparison was made between five i3D of additive manufacturing, two DLP, two by stereolithography (SLA), and one by Fused Material Deposition (FDM), to determine its use in dentistry and its clinical possibilities. The choice of the five i3D was based on their market value, trying to cover most of the Argentinean diversity available. Twenty models were printed with each i3D and scanned with Carestream Intraoral Scanner (IOS) model 3600® (Cs3600). The 120 MMi were imported into the reverse engineering program Geomagic® Control X® (Cx) for 3D analysis, consisting of overlaying MMo with each MMi. Then, a qualitative evaluation of the deviation between MMi and MMo. Also, a careful statistical analysis was performed, resulting in 3d and 2d comparisons. Metrological coincidences in three-dimensional overlay allowed a comprehensive and easily recognizable analysis through colorimetric maps. In the two-dimensional analysis, dentally referenced planes were proposed from the MMo, to match the measurements from the same dental starting point. The results were satisfactory and very encouraging. The probabilities of obtaining ranges of variability equivalent to +/- 50µm were 40.35 % and +/- 100µm 71.04 %. Therefore, considering the demands of clinical dimensional congruence, precision, and accuracy to which our dental profession it is subjected, clinical problems caused by dimensional errors in manufacturing (Cam) are avoided (AU)


Subject(s)
Models, Dental , Printing, Three-Dimensional , Stereolithography , Orthodontics/methods , In Vitro Techniques , Algorithms , Software , Image Interpretation, Computer-Assisted/methods , Data Interpretation, Statistical , Evaluation Studies as Topic
2.
An. Facultad Med. (Univ. Repúb. Urug., En línea) ; 9(1): e203, jun. 2022. graf, tab
Article in Spanish | LILACS, BNUY, UY-BNMED | ID: biblio-1383558

ABSTRACT

La duración de la carrera de medicina en la Universidad de la República, Uruguay, se redujo un año al modificarse el plan de estudios. Estudiantes que cursaron el novel y el antiguo plan rindieron sincrónicamente un concurso previo a la práctica preprofesional en 2015, graduándose simultáneamente. Este trabajo indagó sobre las potenciales derivaciones de cursar uno u otro plan, como forma de obtener insumos para la evaluación del plan de estudios actual y de los programas de becas de apoyo estudiantil, a fin de proyectar estrategias de mejora. Se realizó un estudio retrospectivo cuantitativo analizando el desempeño, la retención y el rezago estudiantil en relación con el plan cursado, atributos sociodemográficos y el usufructo de becas. Los graduados con rezago tuvieron peor desempeño al final de la carrera que los graduados en tiempo, independientemente del plan. El rezago se asoció al perfil sociodemográfico. El 23.7% de los graduados usufructuó alguna beca durante su carrera. Los becados presentaron niveles educativos parentales inferiores y procedieron en mayor proporción del interior del país que el total de graduados. Se concluye que: a) la desvinculación y el rezago académico tienen lugar en ambos planes; b) cursar con rezago se asocia a un peor desempeño en etapas finales de la carrera, vinculándose con el perfil sociodemográfico y no con cursar un plan de estudios u otro y c) las políticas institucionales de becas remedian parcialmente este hecho favoreciendo la retención y graduación.


At the Universidad de la República, Uruguay, the medical career duration was reduced as a consequence of a curriculum renovation. Students who attended the novice and the prior curriculum synchronously took a pre-practice contest in 2015, graduating simultaneously. This work investigated the potential derivations of taking the previous or the new curriculum and to obtain inputs for the evaluation of the current curriculum and student support scholarship programs in order to project improvement strategies. A quantitative retrospective study was carried out analyzing student performance, retention and delayed graduation in relation to the curriculum taken, sociodemographic attributes and the use of scholarships. Regardless of the curriculum, those with a delayed graduation showed lower results than those who graduated in time. Delayed graduation was associated with the sociodemographic profile. A 23.7% of the graduates used a scholarship at some point in their career. The scholarship recipients presented lower parental educational levels and came in a greater proportion from the interior of the country than the total number of graduates. It was concluded that: a) dropout and academic delay occur at both curriculums; b) obtaining a degree with a delay is associated with worse performance in the final career stages, being linked to the sociodemographic profile and not to taking one study plan or another and c) institutional scholarship policies partially solve baseline inequities, favoring retention and graduation.


A duração do curso de medicina da Universidade da República, Uruguai, foi reduzida em um ano como consequência da modificação do plano de estudos. Os alunos que pegaram o novato e o plano antigo de forma síncrona realizaram um concurso anterior a prática pré-profissional em 2015, graduando-se simultaneamente. Este trabalho investigou as possíveis derivações da adoção de um ou outro plano, como forma de obter informaçoes para a avaliação do atual plano de estudos e programas de bolsas de apoio ao estudante, a fim de projetar estratégias de melhoria. Realizou-se um estudo quantitativo retrospectivo analisando desempenho, retenção e defasagem dos alunos em relação ao plano realizado, atributos sociodemográficos e utilização de bolsas. Os graduados com defasagem alcançaram resultados significativamente mais baixos do que os graduados no prazo, independentemente do plano. A defasagem foi associada ao perfil sociodemográfico. Vinte e três por cento dos egressos utilizaram bolsa durante a curso. Os bolsistas apresentavam pais com menor nível educativo e vinham em maior proporção do interior do país do que o total de diplomados. Conclui-se que: a) o desistência e a defasagem acadêmica ocorrem em ambos os planos; b) a defasagem está associado a um pior desempenho nas fases finais da curso, estando vinculado ao perfil sociodemográfico e não à realização de um ou outro plano de estudos e ao perfil sociodemográfico e não de um ou outro plano de estudos e c) as políticas institucionais de bolsas sanam parcialmente este fato, favorecendo a retenção e a graduação.


Subject(s)
Humans , Male , Female , Adolescent , Adult , Young Adult , Students, Medical/statistics & numerical data , Evaluation of Medical School Curriculum , Academic Performance/statistics & numerical data , Socioeconomic Factors , Retrospective Studies , Data Interpretation, Statistical , Evaluation Study , Educational Status , Fellowships and Scholarships/statistics & numerical data
3.
Prensa méd. argent ; 108(3): 165-189, 20220000. tab, fig, graf
Article in Spanish | LILACS, BINACIS | ID: biblio-1373279

ABSTRACT

Introducción. Los adenomas gigantes de hipófisis (AGHs) son aquellos tumores con un diámetro máximo ≥4 cm en cualquier dirección, representando del 5% al 14% del total de los adenomas que se tratan quirúrgicamente. Su manejo terapéutico es desafiante ya que, debido a su extensión hacia otras estructuras cerebrales,está asociado a un riesgo mayor de complicaciones quirúrgicas, con una menor tasa de resección total. El objetivo de este trabajo fue evaluar el impacto de la cirugía endoscópica transesfenoidal en AGHs, analizando las variables asociadas a resultados visuales, endocrinológicos y según el grado de resección. Pacientes y Métodos. Se evaluó en 44 pacientes con AGHs(diámetro ≥4 cm y/o volumen ≥10 ml) la presentación visual, endocrinológica e imágenes. Se analizaron estadísticamente resultados visuales, endocrinológicos, grado de resección y complicaciones quirúrgicas. Resultados. Edad promedio de 48.8 años, 24 mujeres y 20 hombres. Presentación: déficit del campo visual (93.1%), hipopituitarismo (61.3%), cefalea (54.5%). Diámetro, volumen y extensión supraselar promedios: 4.8 cm, 20.3 ml, 1.8 cm, respectivamente. Campo visual: mejoría: 83.3%, sin cambios: 9.5%, mayormente en síntomas bilaterales (p<0.0001). Desmejoríavisual: 0%. En resección total: mayor posibilidad de mejoría visual (p=0.040). Buenos resultados endocrinológicos: 85.7%. Tasa de resección total: 52.3%. Resección subtotal: más frecuente con invasión del seno cavernoso (p=0.014). Sin diferencias en el grado de resección según diámetro, volumen, extensión supraselar, forma ni aspecto. Hipopituitarismo: 4.2%. Diabetes insípida: 9.5%, asociada a mayor diámetro (p=0.038) o extensión supraselar (p=0.010) y aspecto sólido (p=0.023). Fístula de LCR: 7.1%. Conclusión. La resección total puede lograrse en la mitad de los casos, siendo la limitante principal el grado de invasión del seno cavernoso y no el aspecto morfológico del AGH per se. Aun así, los resultados visuales y endocrinológicos son muy buenos. En resecciones incompletas se logra el control de la enfermedad mediante tratamientos complementarios


Introduction. Giant pituitary adenomas (sGAs) are those tumors with a maximum diame- ter ≥4 cm in any direction, representing 5% to 14% of all adenomas that are treated surgi- cally. Its therapeutic management is challenging since, due to its extension to other brain structures, it is associated with a higher risk of surgical complications, with a lower rate of total resection. Te objective of this work was to evaluate the impact of transsphenoidal endoscopic surgery on AGHs, analyzing the variables associated with visual and endocri- nological results and according to the degree of resection. Patients and Methods. Visual, endocrinological and imaging presentation were evaluated in 44 patients with sHAA (dia- meter ≥4 cm and/or volume ≥10 ml). Visual and endocrinological results, degree of resection and surgical complications were statistically analyzed. Results. Average age of 48.8 years, 24 women and 20 men. Presentation: visual field deficit (93.1%), hypopituitarism (61.3%), headache (54.5%). Average diameter, volume and suprasellar extension: 4.8 cm, 20.3 ml, 1.8 cm, respectively. Visual field: improvement: 83.3%, no changes: 9.5%, mostly in bilate- ral symptoms (p<0.0001). Visual impairment: 0%. In total resection: greater possibility of visual improvement (p=0.040). Good endocrinological results: 85.7%. Total resection rate: 52.3%. Subtotal resection: more frequent with invasion of the cavernous sinus (p=0.014). No differences in the degree of resection according to diameter, volume, suprasellar exten- sion, shape or appearance. Hypopituitarism: 4.2%. Diabetes insipidus: 9.5%, associated with greater diameter (p=0.038) or suprasellar extension (p=0.010) and solid appearance (p=0.023). CSF fistula: 7.1%. Conclusion. Total resection can be achieved in half of the cases, the main limitation being the degree of invasion of the cavernous sinus and not the morphological appearance of the HGA per se. Even so, the visual and endocrinological results are very good. In incomplete resections, disease control is achieved through com- plementary treatments


Subject(s)
Humans , Pituitary Gland/pathology , Postoperative Complications , Adenoma/pathology , Chi-Square Distribution , Data Interpretation, Statistical , Endoscopy/methods , Surgical Clearance , Margins of Excision , Hypophysectomy/methods
4.
Rev. Ciênc. Plur ; 8(2): e23767, mar. 2022. tab, graf
Article in Portuguese | LILACS, BBO | ID: biblio-1367887

ABSTRACT

Introdução: Ambientes urbanos como áreas verdes, praças, parques e escolas estão se tornando cada vez mais propícios ao aparecimento de animais peçonhentos, dentre eles as lagartas urticantes. Acidentes com algumas lagartas urticantes podem ser graves, podendo leva ao óbito, como no caso do gênero Lonomia.Objetivo:descrever o perfil epidemiológico dos acidentes envolvendo lagartas urticantes no município de Chapecó, Santa Catarina e identificar espécies vegetais hospedeiras destas lagartas.Metodologia:Foram coletados os dados epidemiológicos dos acidentes com lagartas urticantes registrados no período entre 2016 e 2017. Os dados foram obtidos junto ao Sistema de Informação de Agravos de Notificação.Foram analisadas as variáveis sexo, idade, parte do corpo acometida, os meses que houve as notificações, a ocupação dos indivíduos que tiveram contato com a lagarta, local em que residem, escolaridade e a espécie da lagarta envolvida. Ainda, foram identificados os espécimes coletados pelo setor de Vigilância em Saúde Ambiental, bem como foram identificadas as plantas hospedeira sem que foram encontrados.Resultados:Foram registrados 377 acidentes envolvendo majoritariamente o sexo feminino e a faixa etária entre 20 a 49 anos.As partes do corpo mais acometidas foram o pé (17,0%), mão (12,9%) e cabeça (11,4%). Foram identificadas seis espécies de plantas associadas à ocorrência de lagartas urticantes Conclusões:O estudo contribui com informações epidemiológicas sobre os acidentes causados por lepidópteros em um município de grande porte. Ressalta-se, a importância de ampliar as pesquisas em relação às plantas hospedeiras e a divulgação dos resultados visando a prevenção de acidentes (AU).


Introduction:Urban environments such as green areas, squares, parks and schools are becoming more and more favorableto the appearance of venomous animals, including urticatingcaterpillars. Accidents with some stinging caterpillars can be serious and can lead to death, as in the case of the genus Lonomia. Objective:to describe the epidemiological profile of accidents involving stinging caterpillars in the municipality of Chapecó, Santa Catarina and to identify host plant species of these caterpillars. Methodology:Epidemiological data on stinging caterpillar accidents recorded in the period between 2016 and 2017were collected. The data were obtained from the Sistema de Informação de Agravos de Notificação. The variables sex, age, body part affected, the months of the notifications, the occupation of the individuals who had contact with the caterpillar, the place where they live, education and the species of the caterpillar involved were analyzed. Still, the specimens collected by the Environmental Health Surveillance sector were identified, as well as the host plants in which they were found. Results:There were 377 accidents involving mostly females and the age group between 20 and 49 years. The parts of the body most affected were the foot (17.0%), hand (12.9%) and head (11.4%). Six species of plants were identified associated with the occurrence of stinging caterpillars.Conclusions:The study contributes with epidemiological information on accidents caused by Lepidoptera in a large municipality. The importance of expanding research in relation to host plants and the dissemination of results aimed at preventing accidents are emphasized (AU).


Introducción: Los entornos urbanos como áreas verdes, plazas, parques y escuelas son cada vez más propicios para la aparición de animales venenosos, incluidas las orugas urticantes. Los accidentes con algunas orugas pueden ser graves y provocar la muerte, como en el caso del género Lonomia.Objetivo: describir el perfil epidemiológico de los accidentes por orugasurticantes en el municipio de Chapecó, Santa Catarina e identificar especies de plantas hospedadoras de estas orugas.Metodología: Se recolectaron datos epidemiológicos sobre accidentes de orugas urticantes registrados en el período entre 2016 y 2017. Los datos se obtuvieron del Sistema de Informação de Agravos de Notificação(SINAN). Se analizaron las variables sexo, edad, parte del cuerpo afectada, los meses delas notificaciones, la ocupación de los individuos que tuvieron contacto con lasorugas, el lugar donde viven, educación y la especie de oruga involucrada. Aún así, se identificaron los especímenes recolectados por el sector de Vigilancia de Salud Ambiental, así como las plantas hospedantes en las que se encontraron.Resultados: Se produjeron 377 accidentes que afectaron en su mayoría a mujeres y al grupo de edad entre 20 y 49 años. Las partes del cuerpo más afectadas fueron el pie (17,0%), mano (12,9%) y cabeza (11,4%).Se identificaron seis especies de plantas asociadas con la ocurrencia de orugas urticantes.Conclusiones: El estudio presentainformación epidemiológica sobre accidentes causados por lepidópteros en un municipio de gran tamaño. Se destacala importancia de ampliar la investigación en relación con las plantas hospedantes y la difusión de resultados orientados a la prevención delosaccidentes (AU).


Subject(s)
Humans , Male , Female , Adult , Middle Aged , Health Profile , Public Health Surveillance/methods , Accident Prevention/instrumentation , Lepidoptera , Animals, Poisonous , Brazil/epidemiology , Data Interpretation, Statistical
5.
Rev. Ciênc. Plur ; 8(2): e25243, mar. 2022. tab
Article in Portuguese | LILACS, BBO | ID: biblio-1368175

ABSTRACT

Introdução:As doenças do aparelho respiratório se configuram como o segundo principal motivo de internações hospitalares no Brasil entre 2013 e 2017. Objetivo:Analisar a morbimortalidade de doenças do aparelho respiratório da população brasileira, segundo faixa etária,no período compreendido entre os anos de 2015 a 2019. Metodologia:Trata-se de um estudo do tipo ecológico, retrospectivo, realizado sobre o território brasileiro. Os dados foram coletados a partir do Departamento de Informática do Sistema Único de Saúde, nas seções de Morbidade Hospitalar, de Mortalidade e População Residente. Foram analisados os dados entre 2015 a 2019 e de todas as faixas etárias. Resultados:Ao investigar a mortalidade por doenças do sistema respiratório entre os anos de 2015 e 2019, as cinco causas mais frequentes foram: influenza e pneumonia; doenças crônicas das vias aéreas inferiores; outras doenças do aparelho respiratório; outras doenças respiratórias que afetam principalmente interstício; doenças pulmonares devidas a agentes externos, nessa ordem. Enquanto as cinco causas de morbidades mais frequentes foram: pneumonia; outras doenças do aparelho respiratório; bronquite, enfisema e outras doenças pulmonares obstrutivas crônicas; asma; bronquite aguda e bronquiolite aguda. Conclusões:Verificou-se que a pneumonia, influenza, doenças respiratórias do trato inferior e outras doenças crônicas respiratórias foram as mais prevalentes entre a população respectivamente. Dentre o público mais acometido, foi possível constatar que o público infantil e a população idosa foram os mais atingidos tanto na mortalidade quanto na morbidade (AU).


Introduction:Introduction: Respiratory diseases are the second main reason for hospital admissions in Brazil between 2013 and 2017. Objective:To analyze the morbidity and mortality of respiratory diseases of the Brazilian population, according to age group, in the period from 2015 to 2019. Methodology:This is an ecological, retrospective study conducted on the Brazilian territory. Data were collected from the Informatics Department of the Unified Health System, in the Sections of Hospital Morbidity, Mortality and Resident Population. Data were analyzed between 2015 and 2019 and all age groups. Results:When investigating mortality from respiratory system diseases between 2015 and 2019, the five most frequent causes were: influenza and pneumonia; chronic diseases of the lower airways; other diseases of the respiratory system; other respiratory diseases that mainly affect interstitium; diseases due to external agents, in that order. Conclusions:It was found that pneumonia, influenza, respiratory diseases of the lower tract and other chronic respiratory diseases were the most prevalent among the population, respectively. Among the most affected public, it was possible to observe that the child's public and the elderly population were the most affected in both mortality and morbidity (AU).


Introducción: Las enfermedades respiratorias son la segunda razón principal de los ingresos hospitalarios en Brasil entre 2013 y 2017. Objetivo: Analizar la morbilidad y mortalidad de las enfermedades respiratorias de la población brasileña, según el grupo de edad, en el período comprendido entre 2015 y 2019.Metodología: Se trata de un estudio ecológico y retrospectivo realizado en territorio brasileño. Los datos fueron recogidos del Departamento de Informática del Sistema Unificado de Salud, en las Secciones de Morbilidad Hospitalaria, Mortalidad y Población Residente. Los datos se analizaron entre 2015 y 2019 y todos los grupos de edad. Resultados: Al investigar la mortalidad por enfermedades del sistema respiratorio entre 2015 y 2019, las cinco causas más frecuentes fueron: gripe y neumonía; enfermedades crónicas de las vías respiratorias inferiores; otras enfermedades del sistema respiratorio; otras enfermedades respiratorias que afectan principalmente al intersticio; enfermedades debidas a agentes externos, en ese orden.Mientras que las cinco causas más frecuentes de morbilidad fueron: neumonía; otras enfermedades del sistema respiratorio; bronquitis, enfisema y otras enfermedades pulmonares obstructivas crónicas; asma; bronquiolitis aguda y bronquiolitis aguda. While the five most frequent causes of morbidities were: pneumonia; other diseases of the respiratory system; bronchitis, emphysema and other chronic obstructive pulmonary diseases; asthma; acute bronchitis and acute bronchiolitis. Conclusiones: Se encontróque la neumonía, la influenza, las enfermedades respiratorias de las vías inferiores y otras enfermedades respiratorias crónicas eran las más prevalentes entre la población, respectivamente. Entre el público más afectado, se pudo observar que el público del niño y la población de edad avanzada eran los más afectados tanto en la mortalidad como en la morbilidad (AU).


Subject(s)
Humans , Male , Female , Respiratory System/anatomy & histology , Respiratory Tract Diseases/pathology , Brazil/epidemiology , Indicators of Morbidity and Mortality , Retrospective Studies , Data Interpretation, Statistical , Ecological Studies , Age Groups
6.
Rev. ADM ; 79(1): 12-19, ene.-feb. 2022. ilus, graf
Article in Spanish | LILACS | ID: biblio-1361307

ABSTRACT

El objetivo del presente estudio fue medir la curvatura vestíbulo-lingual de los conductos de las raíces mesiales de primeros molares inferiores en personas adultas del estado de Chihuahua, por medio de la técnica de tomografía computarizada de haz cónico (CBCT, por sus siglas en inglés) y usando el software de análisis 3D EndoTM (Dentsply/Sirona USA). Se llevó a cabo la medición por dos observadoras, empleando dos métodos diferentes, Schneider y 3D EndoTM (Dentsply/Sirona USA). Los resultados obtenidos tuvieron valores mayores a los 100 grados con la técnica 3D EndoTM y a los 20 grados con la técnica de Schneider. No se detectó una diferencia estadística significativa al comparar los diferentes conductos entre sí (AU)


The objective of the present study was to measure the vestibule-lingual curvature of the ducts of the mesial roots of lower first molars, of adults from the state of Chihuahua. Using the cone beam computed tomography (CBCT) technique and using the 3D analysis software EndoTM (Dentsply/Sirona USA). The measurement was carried out by two observers using two different methods, Schneider and 3D EndoTM (Dentsply/Sirona USA). The results obtained had values greater than 100 degrees with the 3D EndoTM technique and at 20 degrees with the Schneider technique. No statistically significant difference was detected when comparing the different ducts with each other (AU)


Subject(s)
Humans , Dental Pulp Cavity , Cone-Beam Computed Tomography , Molar , Software , Data Interpretation, Statistical , Imaging, Three-Dimensional , Mexico
7.
Rev. ADM ; 79(1): 20-27, ene.-feb. 2022. ilus, tab
Article in Spanish | LILACS | ID: biblio-1361506

ABSTRACT

Los sistemas cementantes han mejorado notablemente, los objetivos que persiguen los nuevos cementos es que la adhesión sea duradera y conseguir siempre que sea posible una interface cerrada con un sellado perfecto. Se han podido desarrollar nuevas técnicas y nuevos materiales de cementación que han ido perfeccionando la unión del material restaurador al diente. En el presente estudio se compara la fuerza de adhesión a dentina de cementos de autograbado y cementos de grabado total para comprobar los efectos positivos en el grabado de la dentina. Para el estudio se utilizaron dos cementos a base de resina (RelyX U200 Clicker 3M y RelyX Ultimate 3M). Se encapsularon 40 molares en acrílico en dos grupos de 20 muestras para la aplicación de dos sistemas cementantes de autograbado (grupo 1) y de grabado total (grupo 2), respectivamente, se desgastaron hasta descubrir la dentina; siguiendo las especificaciones del fabricante se colocó el cemento en cada grupo, y después se sometieron a pruebas de cizalla en una máquina de ensayo universal Instron. La medida expresada en megapascales (MPa) fue: grupo 1 = 7.5569 y grupo 2 = 12.6444. En este caso fueron analizados dos grupos, tomándose la primera significancia bilateral. Se realiza la prueba en t de Student, con 95% de intervalo de confianza en la diferencia, demostrando así que el cemento RelyX Ultimate 3M tiene mayor fuerza de adhesión que el cemento RelyX U200 Clicker 3M. Nuestra investigación fue factible y llegamos a nuestro propósito, en el cual comprobamos la mayor adhesión de cementos de grabado total, los cuales son sometidos a un previo tratamiento del diente (AU)


Subject(s)
Humans , Adolescent , Adult , Acid Etching, Dental , Cementation , Dentin-Bonding Agents , Resin Cements , Shear Strength , Tensile Strength , Data Interpretation, Statistical , Dentin/drug effects
8.
Rev. ADM ; 79(1): 38-47, ene.-feb. 2022. tab
Article in Spanish | LILACS | ID: biblio-1361906

ABSTRACT

Las urgencias odontológicas son, quizá, las razones principales de atención en el consultorio, muchas veces el significado de dolor se encuentra acompañado por inflamación; el uso de antiinflamatorios no esteroideos (AINES) es común en el ejercicio de la odontología por la excelente respuesta analgésica y antiinflamatoria que tiene, por lo que es importante conocer la fisiopatología de la inflamación y el dolor y cómo actúan los AINES, ya que algunos de estos fármacos tienen respuestas adversas y sitios de acción importantes. Los factores de riesgo por inflamación y dolor nos obligan a conocer la variedad de fármacos que no entran en la clasificación de AINES y que tenemos a disposición, hay más opciones para la elección ante la presencia de inflamación por un factor en particular, cada uno de éstos tienen indicaciones y contraindicaciones que conoceremos, lo cual nos ampliará el conocimiento para dar una prescripción ante la presencia de eventos inflamatorios. Se realizó un estudio detallado de artículos bibliográficos de cada tema, los fármacos más usados en odontología son los AINES, hay poco uso y conocimiento de antiinflamatorios que podemos usar en urgencias, el porcentaje de uso de los AINES derivados del ácido propiónico es alto por la excelente respuesta en pacientes y otras veces por el desconocimiento de más opciones (AU)


Dental emergencies are perhaps the main reasons for care in the office, many times the meaning of pain is accompanied by inflammation, the use of non-steroidal anti-inflammatory drugs is common in the practice of dentistry due to the excellent analgesic and anti-inflammatory response it has, important is knowing the pathophysiology of inflammation and pain, how NSAIDs act, some of these drugs have adverse responses and important sites of action, risk factors for inflammation and pain require us to know the variety of drugs that do not enter the classification of NSAIDs and we have at our disposal, there are more options for choosing in the presence of inflammation due to a particular factor, each of these have indications and contraindications that we will know, it expands our knowledge to give a prescription in the presence of inflammatory events. A detailed study of bibliographic articles on each topic was carried out, the drugs most used in dentistry are NSAIDs, there is little use and knowledge of anti-inflammatories that we can use in the emergency room, the percentage of use of NSAIDs derived from propionic acid is high, due to the excellent response in patients and others due to lack of knowledge of more options (AU)


Subject(s)
Humans , Male , Female , Toothache , Pharmaceutical Preparations , Anti-Inflammatory Agents, Non-Steroidal , Inflammation , Pain/pathology , Pain, Postoperative , Propionates , Prostaglandins/physiology , Data Interpretation, Statistical , Drug Interactions , Cyclooxygenase 1/pharmacology , Cyclooxygenase 2 , Cyclooxygenase 2 Inhibitors , Narcotics
9.
Acta sci., Health sci ; 44: e57616, Jan. 14, 2022.
Article in English | LILACS | ID: biblio-1366131

ABSTRACT

This study evaluated the effect of contamination of composite resins (CRs) handled by undergraduate students during restorative procedures, varying the time (baseline, 30 days and 60 days) and experimental condition (before and after handling, contamination with saliva [positive control] and photoactivation). Eight CR tubes were randomly distributed at the dental clinic and the samples were organized into four groups: CR fragments collected before (GB) and after (GA) the restorative procedure; CR fragments contaminated with saliva (GS) and photoactivated (GP) both collected after the procedure. These 4 groups were evaluated in 3 different times: baseline (after sealing), 30 days and 60 days of use of the CR. Samples that had positive turbidity in Brain HeartInfusion (BHI) broth were sown in BHI and Sabouraud Dextrose (SB) agars for subsequent counting of Colony Forming Units (CFU mL-1). The results showed that the handling was responsible for increasing contamination (p < 0.05) at the baseline (GB [n = 0] and GA [n = 3]), as well as after 30 (GB [n = 1] and GA [n = 6]) and 60 (GB [n = 1] and GA [n = 5]) days of use. Photoactivation was responsible for the reduction for microorganisms in T0 and T60. Additionally, the time use and conservation did not influencethe contamination of CRs. Handling was responsible for the increase of contamination of CR, the photoactivation seems to reduce the number of viable microorganisms and the time of use seems not to potentiate the effect of tube contamination.


Subject(s)
Pollution Indicators , Composite Resins/analysis , Good Manipulation Practices , Light-Curing of Dental Adhesives/instrumentation , Students, Dental , Data Interpretation, Statistical , Environmental Restoration and Remediation , Food Preservatives/analysis , Microbiology/instrumentation
10.
Article in English | LILACS, BBO | ID: biblio-1365228

ABSTRACT

ABSTRACT Objective To evaluate knowledge and attitudes towards biosafety recommendations during the COVID-19 pandemic at a Brazilian dental school. Material and Methods A cross-sectional study was performed in 2020 with the clinical staff of a Brazilian dental school. The whole clinical staff was sent pre-tested self-administered online questionnaires about knowledge and attitudes towards the recommendations for biosafety in dental settings in the context of the COVID-19 pandemic. Descriptive statistical analyses were carried out for proportion calculation. Results Disposable head covering caps, isolation gowns, and gloves were the most frequently reported personal protective equipment (PPE). The rates ranged from 52.9% to 88.5% for N95 respirators, from 68.6% to 92.6% for face shields, from 47.4% to 67.5% for conventional eye protection shields, and 45.1% to 77.4% for eye protection with solid side shields. Chlorhexidine gluconate was the most frequent mouthwash indicated before clinical dental care. The percentage of agreement to provide clinical care to patients with suspected COVID-19 varied from 23.5% to 50.0%. The percentage of respondents who agreed that bioaerosol-generating procedures should be avoided was higher than 74.5%. Less than 50% knew the correct sequence for doffing of PPE. Conclusion This study revealed important gaps in knowledge and attitudes towards prevention and control measures against infection in dental environments in the context of COVID-19, indicating the need for improvements.


Subject(s)
Humans , Brazil , Health Knowledge, Attitudes, Practice , Containment of Biohazards/instrumentation , Education, Dental , Personal Protective Equipment , COVID-19 , Schools, Dental , Epidemiologic Studies , Cross-Sectional Studies/methods , Surveys and Questionnaires , Data Interpretation, Statistical , Infection Control
11.
Pesqui. bras. odontopediatria clín. integr ; 22: e210046, 2022. tab, graf
Article in English | LILACS, BBO | ID: biblio-1365231

ABSTRACT

ABSTRACT Objective To describe and evaluate the xylitol products' applicability and its effects in the health area worldwide utilizing a bibliometric analysis from randomized controlled trials (RCT) with humans. Material and Methods Electronic searches were carried out in Medline/PubMed, Scopus, Cochrane Library, Web of Science, and VHL databases. The main data extracted were: year, area of applicability, type of treatment, country, journal, xylitol posology and concentration, presentation form, outcomes, and effects. Results From 1476 studies, 257 were included. These studies were published between 1973-2021. The majority was carried out in dentistry (73.9%) and under preventive treatment (67.4%). These studies were developed in the USA (15.4%) and published in Caries Research (6.6%). The posology and concentration ranged between 0.004-67 g/day and 0.002-100%, respectively. The xylitol is usually used in the chewing gum form (44.0%), and for antimicrobial activity evaluation (38.5%). A positive effect was observed in 204 studies (79.3%) and was associated with xylitol concentration ≥ 15(p=0.007). Side effects were reported in 8.2and were associated with posology ≥ 5 g/day (p=0.03). Conclusion Most studies with xylitol were conducted to prevent diseases in the dentistry field. The chewing gum form and antimicrobial activity evaluation were more frequent. Most xylitol products have a positive effect, and few studies report side effects.


Subject(s)
Therapeutics , Xylitol/therapeutic use , Bibliometrics , Randomized Controlled Trials as Topic , Chewing Gum , Anti-Infective Agents , Brazil , Chi-Square Distribution , Oral Health/education , Data Interpretation, Statistical
12.
Pesqui. bras. odontopediatria clín. integr ; 22: e200192, 2022. tab, graf
Article in English | LILACS, BBO | ID: biblio-1365232

ABSTRACT

Abstract Objective: To evaluate the effectiveness of Antimicrobial Photodynamic Therapy (aPDT), associated with scaling and root planing in the non-surgical periodontal treatment of individuals with Down Syndrome. Material and Methods: A controlled, randomized, split-mouth study was conducted. A total of 8 participants diagnosed with Down Syndrome aged 17-38 years of both sexes with clinical periodontitis were included in the study. Participants were treated at least three times: at the baseline, Plaque Index (PI), Bleeding on Probing (BOP), and Probing Pocket Depth (PPD) were obtained. After one week, conventional scaling and root planing were performed, and two quadrants were randomly selected for aPDT application. The reassessment was obtained one month after the aPDT application. The significance level was set at 5%. Analyses were performed considering a 95% confidence interval. Results: In the intergroup evaluation, no statistically significant differences were observed (p>0.05). In the intragroup evaluation, no statistically significant variations were observed in relation to the PI (p>0.05) and PPD (p>0.05); however, a statistically significant reduction in the BOP was observed between the test group (p=0.013) and control group (p=0.015). Conclusion: The use of aPDT as adjuvant therapy did not promote additional benefits in decreasing PI and PPD after 1 month of treatment. However, a significant reduction in the BOP was observed in the intragroup evaluation.


Subject(s)
Humans , Male , Female , Adolescent , Adult , Periodontal Diseases/pathology , Periodontitis , Photochemotherapy/instrumentation , Periodontal Index , Down Syndrome/pathology , Brazil/epidemiology , Dental Plaque Index , Data Interpretation, Statistical , Randomized Controlled Trial , Treatment Outcome , Caregivers , Statistics, Nonparametric , Lasers
13.
Pesqui. bras. odontopediatria clín. integr ; 22: e210106, 2022. tab, graf
Article in English | LILACS, BBO | ID: biblio-1365239

ABSTRACT

ABSTRACT Objective To evaluate the physical conditions and presence of residues of toothbrushes used by mothers and their babies and mothers' knowledge about toothbrush care. Material and Methods This was a cross-sectional study comprising a convenience sample represented by 60 mother-baby pairs. The mothers answered a questionnaire to evaluate their knowledge of toothbrush care. A calibrated dentist performed a visual inspection of the toothbrushes. Statistical analysis was performed using the chi-square and Fisher's exact tests, with a significance level of 5%. Results It was found that 82% of the mothers had never received instructions regarding the care of toothbrushes after use (p=0.024). Most of them believed that their toothbrushes (70%) and their children's toothbrushes (88%) were in good condition to use (p=0.043). However, most mother's toothbrushes presented an unacceptable deformity of the bristles (65%) and the presence of residues (60%). In addition, babies' toothbrushes also presented unacceptable deformities of the bristles (52%) and residues (55%). There was an association between the lack of instructions received by the mother and the presence of deformity and residues on the mother's toothbrush bristles (p=0.037 and p=0.003, respectively). Conclusion Most mothers had never received instructions regarding toothbrush care, which is reflected in the condition of their and their baby's toothbrushes, which presented unacceptable physical conditions concerning deformation and presence of residues.


Subject(s)
Humans , Female , Infant , Child, Preschool , Adult , Oral Hygiene , Toothbrushing/methods , Health Knowledge, Attitudes, Practice , Dental Devices, Home Care , Home Nursing , Brazil/epidemiology , Chi-Square Distribution , Cross-Sectional Studies/methods , Surveys and Questionnaires , Data Interpretation, Statistical , Dentists , Observational Study , Infant , Mothers
14.
Article in English | LILACS, BBO | ID: biblio-1365238

ABSTRACT

Abstract Objective: To verify the association between impacts produced by tooth loss and sociodemographic variables in the oral health-related quality of life (OHRQoL) in the elderly. Material and Methods: This is an analytical cross-sectional study carried out in the municipality of Vitória/ES, Brazil, which sample was composed of 402 older adults. The Oral Health Impact Profile (OHIP-14) was used to measure the individuals' OHRQoL. For data analysis, descriptive and bivariate analyses were performed using the Fisher's exact test and the Mantel-Haenzsel test to assess the effects of combined dimensions. The significance level adopted was 5%. To assess the strength of association between independent variables and dimensions, Odds-Ratio (OR) with 95% confidence interval (CI) was calculated. Results: The prevalence of negative impact on OHRQoL in the elderly was 32.6%. The greatest impact perception was found in individuals with up to 10 years of schooling (p=0.021 and OR=1.602), with need for removable partial dentures (p=0.000 and OR=2.873) and those who did not accept the loss of dental elements (p=0.000 and OR=3.064). Conclusion: Older female adults with socioeconomic class C/D-E, with up to 10 years of schooling, who lost 11 or more teeth, who declared the need for removable partial dentures, suffered greater impacts caused by tooth loss on OHRQoL.


Subject(s)
Humans , Male , Female , Middle Aged , Aged , Aged, 80 and over , Quality of Life/psychology , Aged , Oral Health/education , Dental Care for Aged , Tooth Loss/pathology , Sickness Impact Profile , Social Class , Brazil/epidemiology , Demography , Cross-Sectional Studies/methods , Surveys and Questionnaires , Data Interpretation, Statistical , Mouth, Edentulous , Denture, Partial, Removable
15.
Article in English | LILACS, BBO | ID: biblio-1365233

ABSTRACT

ABSTRACT Objective To determine the relationship between dental anxiety and self-reported periodontal status. Material and Methods The study was conducted among 263 patients at the Family Medicine Clinic of the Lagos State University Teaching Hospital (LASUTH), Ikeja, Lagos, Nigeria. Self-assessed gingival and periodontal disease was measured using the validated periodontal disease self-report surveillance questionnaire. The Modified Dental Anxiety Scale (MDAS) was used for evaluating dental anxiety. Student's t-test was used to test for association between categorical variables. P-value < 0.05 was considered to be statistically significant. Results Seventy-seven (29.3%) participants had high dental anxiety, while 49 (18.6%) had very high dental anxiety. The prevalence of periodontal disease was higher among those with very high dental anxiety (77.6%), while female respondents had a significantly higher proportion of self-reported periodontitis (34.1%). There was a significantly higher prevalence of very high dental anxiety among respondents who had never visited the dentist (23.2%). Similarly, females (19.4%), middle-class respondents (30.8%), and those with a primary level of school education (23.5%) had a higher prevalence of high dental anxiety, even though the association was not significant. Conclusion The prevalence of self-reported periodontal disease among the respondents with very high dental anxiety was higher than in those with high dental anxiety and those without dental anxiety, but the difference was not significant.


Subject(s)
Humans , Male , Female , Adult , Periodontal Diseases/prevention & control , Oral Health/education , Dental Anxiety , Self Report , Nigeria/epidemiology , Periodontitis , Prevalence , Multivariate Analysis , Surveys and Questionnaires , Regression Analysis , Data Interpretation, Statistical , Dentists
16.
Ethiop. j. health dev. (Online) ; 36(2): 1-10, 2022-06-07. Figures, Tables
Article in English | AIM | ID: biblio-1380424

ABSTRACT

Environmental concerns are increasing in and around us due to improper discharge of personal protective gear or equipment (PPEs) during the current pandemic with SARS-CoV-2.The residents of Salalah, under the Dhofar governorate of Oman,were hastening to take every possible measure to safeguard their health against the COVID-19 pandemic. In this scenario, improper discard of facemasks in the environment entails a significant problem forpublic health and aquatic environments. Objective:This study aimsto assess how the SARS-CoV-2 virus disrupted the household waste management chainin the Sultanate of Oman. In addition, descriptivesurvey has also identified people's perception about the existing household waste management system. Methods:Total 200 respondents were personally selected under the purposive sampling category. Data were analyzed using SPSS version 26. The mean, standard deviation, and distribution shapewere calculated based on the retrieved data. The variables and frequencies were tabulated for categorical variables. Results show negative impacts on the environment, wildlife, and public health. It was also observed that there was a significant difference when grouped according to residence location since the obtainedalso observed a significant difference when grouped according to residence location since thep-value of 0.007 was less than 0.05 alpha level. This means that the responses differ significantly. It shows from the test conducted that participantsfrom the village experienced and observed a negative impact on the discarded face masks comparedto those in the city.Conclusion and recommendation: This study illustrates the real impact of the COVID-19 facemasks on the environment, wildlife, and public health. In addition, the new management of the user's facemasks for eliminating or reducing the risks to human health and the environmenthas been suggested. [Ethiop. J. Health Dev. 2022; 36(2):000-000]


Subject(s)
Environmental Health , Data Interpretation, Statistical , Microplastics , COVID-19 , Health Care Surveys , N95 Respirators , SARS-CoV-2
17.
Pesqui. bras. odontopediatria clín. integr ; 22: e210015, 2022. tab, graf
Article in English | LILACS, BBO | ID: biblio-1365235

ABSTRACT

ABSTRACT Objective To determine the level of knowledge of dentistry students regarding the COVID-19 outbreak and to evaluate whether the risk factors of the outbreak cause depression. Material and Methods This study was conducted with 516 dentistry students (3rd, 4th and 5th grades) who started clinical practice in their education. The first part of the questionnaires applied to the students includes questions about demographic information, while the second part contains questions to determine their knowledge levels on the COVID-19 pandemic and protection against it. In the third section, Beck Depression Inventory (BDI) was used to examine depression symptoms. The data were analyzed by frequency analyses and Chi-squared tests via the IBM SPSS 22.0 software. Results Of the 516 students, 150 (29%) participants were in the 3rd, 212 (41%) were in the 4th and 154 (30%) were in the 5th grade. 355 (69%) students stated that their knowledge level about COVID-19 was high. 30.9% were conscious of the importance of using masks. 29.6% were aware of the importance of using gloves, 30.3% of not shaking hands. According to BDI scores, 37.1% of the participants seemed to experience moderate to severe and very severe depression. Conclusion It was observed that the students didn't have a sufficient level of knowledge about the factors affecting the spread of the pandemic. New stress factors such as COVID-19 may increase the incidence of depression.


Subject(s)
Humans , Male , Female , Students, Dental , Health Knowledge, Attitudes, Practice , Risk Factors , Infection Control , Education, Dental , COVID-19 , Turkey/epidemiology , Chi-Square Distribution , Cross-Sectional Studies/methods , Surveys and Questionnaires , Data Interpretation, Statistical , Statistics, Nonparametric , Depression
18.
Pesqui. bras. odontopediatria clín. integr ; 22: e200087, 2022. tab, graf
Article in English | LILACS, BBO | ID: biblio-1365236

ABSTRACT

Abstract Objective: To evaluate the direct and oral impact-mediated association between reduced dentitions and the self-perceived need for complete dentures (CD) in dentate adults. Material and Methods: Data from the Brazilian Oral Health Survey (2010) were analyzed. The outcome was self-perceived need for CD. Functional dentition (FDClassV) was defined by the presence of the following criteria: level I - ≥1 tooth in each arch, level II - 10 teeth in each arch, level III - 12 anterior teeth, level IV - ≥ 3 posterior occluding pairs (POPs) of premolars and level V - ≥1 bilateral POPs of molars. Oral impacts were assessed with Oral Impacts on Daily Performances scale. Results: FDClassV was associated with a less self-perceived need for CD both directly and mediated by oral impacts. Dentitions without level V were associated with the outcome mediated by oral impacts. Between individuals with 10 teeth in each arch, self-perceived need for CD was similar for those who had or not anterior teeth and POPs. Individuals with <10 teeth in each arch and level III did not have a higher frequency of self-perceived need for CD compared to those with level II. Conclusion: Oral impacts mediated the association between reduced dentitions and self-perceived need for CD. Individuals with tooth loss may report need for CD, even when they have dental configurations compatible with functionality.


Subject(s)
Humans , Adult , Middle Aged , Quality of Life , Self Concept , Brazil/epidemiology , Patient Satisfaction , Dental Prosthesis/instrumentation , Dental Arch , Chi-Square Distribution , DMF Index , Oral Health , Cross-Sectional Studies , Surveys and Questionnaires , Data Interpretation, Statistical , Information Storage and Retrieval
19.
Rev. baiana enferm ; 36: e43082, 2022. tab, graf
Article in Portuguese | LILACS, BDENF | ID: biblio-1376468

ABSTRACT

Objetivo: analisar a caracterização dos casos de tuberculose notificados na região Centro-Oeste, no período de 2010 a 2019. Método: estudo ecológico, longitudinal, que utilizou, para análise estatística, o programa Microsoft Excel 2010 (cálculo das frequências) e o teste Chi quadrado (X2) (verificar se as frequências diferiam). Resultados: observou-se maior prevalência da tuberculose em pacientes do sexo masculino (70,8%), pertencentes à faixa etária entre 25-34 anos (23,6%), da raça/cor parda (53%), moradores da zona urbana (83%) e que não tiveram sua escolaridade declarada (28,6%). Ao considerar aspectos epidemiológicos, destacaram-se casos novos (82,6%), na forma pulmonar (85,6%), com soropositividade negativa ao HIV (60,4%) e que não tiveram declarado o hábito de tabagismo (51,1%). Conclusão: os casos de tuberculose notificados na região Centro-Oeste assemelham-se aos encontrados na literatura, reforçando que a tuberculose é uma doença infecciosa atual e sua erradicação ainda exige muito trabalho e políticas públicas efetivas.


Objetivo: analizar la caracterización de los casos de tuberculosis reportados en la región Medio Oeste, en el período de 2010 a 2019. Método: estudio ecológico, longitudinal, que utilizó, para el análisis estadístico, el programa Microsoft Excel 2010 (cálculo de frecuencias) y la prueba de Chi cuadrado (X2) (comprobar si las frecuencias diferían). Resultados: se observó una mayor prevalencia de tuberculosis en pacientes varones (70,8%), con edades comprendidas entre 25-34 años (23,6%), mestizos/color (53%), residentes urbanos (83%) y a quienes no se les declaró la escolaridad (28,6%). Al considerar los aspectos epidemiológicos, se destacaron los nuevos casos (82,6%), en la forma pulmonar (85,6%), con seropositividad VIH negativa (60,4%) y que no habían declarado fumar (51,1%). Conclusión: los casos de tuberculosis reportados en la región del Medio Oeste son similares a los encontrados en la literatura, reforzando que la tuberculosis es una enfermedad infecciosa actual y su erradicación aún requiere mucho trabajo y políticas públicas efectivas.


Objective: to analyze the characterization of tuberculosis cases reported in the Midwest region, in the period from 2010 to 2019. Method: ecological, longitudinal study, which used, for statistical analysis, the Microsoft Excel 2010 program (calculation of frequencies) and the Chi square test (X2) (check if the frequencies differed). Results: a higher prevalence of tuberculosis was observed in male patients (70.8%), aged between 25-34 years (23.6%), mixed race/color (53%), urban residents (83%) and who did not have their schooling declared (28.6%). When considering epidemiological aspects, new cases (82.6%), in the pulmonary form (85.6%), with HIV-negative seropositivity (60.4%) and who had not declared smoking (51.1%) were highlighted. Conclusion: the cases of tuberculosis reported in the Midwest region are similar to those found in the literature, reinforcing that tuberculosis is a current infectious disease and its eradication still requires a lot of work and effective public policies.


Subject(s)
Humans , Male , Female , Tuberculosis, Pulmonary/epidemiology , Disease Notification/statistics & numerical data , Health Information Systems , Data Interpretation, Statistical
20.
São Paulo; s.n; s.n; 2022. 86 p. tab, graf.
Thesis in Portuguese | LILACS | ID: biblio-1378701

ABSTRACT

Responsável por milhões de óbitos anuais e um grande custo para a saúde pública, o câncer é a segunda maior causa de mortes no mundo. Dentre seus diversos tipos, o câncer de pulmão, além da alta incidência, é um dos mais letais. A exposição a substâncias tóxicas provenientes da combustão de matéria orgânica, assim como o consumo de cigarro, são os principais responsáveis pela alta incidência de câncer de pulmão. Dentre estas substâncias, está o benzo[α]pireno (B[α]P), um carcinógeno completo, ou seja, capaz de iniciar e promover o processo de carcinogênese. Resultados anteriores obtidos pelo grupo demonstraram que células BEAS-2B expostas a 1 µM de B[α]P apresentaram alterações das concentrações de metabólitos intracelulares, indução de estresse redox e hipermetilação do DNA. A exposição a 1 µM de nicotinamida ribosídeo (NR), um dos precursores de NAD+, foi capaz de proteger as células BEAS-2B contra a transformação induzida por B[α]P, além de impedir totalmente que células não expostas a B[α]P formassem colônias em soft-agar. A utilização da proteômica neste trabalho permitiu verificar a abundância das proteínas nos quatro diferentes grupos de exposição: Controle, B[α]P, B[α]P + NR e NR. Após 120 h de exposição as células foram coletadas, as proteínas extraídas e preparadas para análise. Foram descobertas 3024 proteínas posteriormente analisadas com o objetivo de elucidar vias possivelmente envolvidas na proteção contra o processo de transfomação maligna. Os grupos NR e Controle demonstram ser mais parecidos em relação ao seu conteúdo, enquanto os grupos B[α]P e B[α]P + NR foram mais semelhantes entre si. A análise de proteínas exclusivas revelou menos processos relacionados ao reparo de DNA no grupo tratado apenas com B[α]P quando comparado com B[α]P + NR. A análise estatística do total de proteínas utilizando o teste ANOVA (p < 0,05, N = 5) revelou 564 proteínas diferencialmente expressas entre os grupos. A clusterização nos permitiu observar a diferença na abundância de proteínas entre os quatro tratamentos. As proteínas estão envolvidas em funções como a regulação do metabolismo, resposta a estresse, transdução de sinal, regulação de expressão gênica e morte celular. Um dos clusters (cluster 1), contendo 59 proteínas, revelou poucos processos na análise de enriquecimento, mas as proteínas contidas nele apresentam funções como controle da divisão celular, apoptose e proteção ao estresse redox. Nele podemos observar que, no geral, o tratamento com B[α]P aumentou a abundância de algumas proteínas, o que foi revertido no grupo B[α]P + NR. O tratamento apenas com NR diminuiu a abundância das proteínas contidas nesse cluster. Outro cluster (cluster 4) apresentou 51 proteínas de abundância diminuída durante a exposição ao B[α]P, o que se reverteu no grupo B[α]P + NR. As proteínas desse cluster estão envolvidas em etapas importantes da via glicolítica, de crescimento, adesão, migração e invasão celular. Apesar de ser descrito que a exposição a NR pode aumentar a eficiência do reparo de DNA, os resultados apresentados nesse trabalho indicam que o efeito protetor pode estar relacionado com a modulação do ciclo celular ou alterações na adesão celular


Responsible for millions of annual deaths and a great health expense, cancer is the second leading cause of death in the world. Among its many types, lung cancer, besides its high incidence, is also one of the most lethal. Exposure to toxic substances resulting from the combustion of organic matter, as well as cigarette consumption, are the mainly responsible for the high incidence of lung cancer. One of these substances is benzo[α]pyrene (B[α]P), a complete carcinogen, able to initiate and promote the carcinogenesis process. Results obtained previously demonstrated that BEAS-2B cells exposed to 1 µM BaP presented alterations in the levels of intracellular metabolites, induction of oxidative stress, and hypermethylation of DNA. The exposure to 1 µM nicotinamide riboside (NR), one of the precursors of NAD+, was able to protect BEAS-2B cells against the transformation induced by B[α]P, moreover, it also totally prevented the colonies formation on soft agar in cells not exposed to B[α]P. The use of proteomics allowed us to verify the abundance of proteins in the four different exposure groups: Control, B[α]P, B[α]P + NR e NR. After 120h of exposure, the cells were collected followed by the extraction of the proteins. A total of 3024 proteins were identified and analyzed aiming to elucidate possible pathways involved in the protective effect against the malignant transformation induced by B[α]P. The NR and Control groups showed to be more similar, while B[α]P and B[α]P + NR were more similar. The analysis of exclusive proteins revealed fewer processes related to DNA repair in B[α]P when compared with B[α]P + NR. The statistical analysis of the total proteins using the ANOVA test (p <0.5, N = 5) revealed 564 proteins differentially expressed between the groups. The heatmap showed the difference in protein abundance between the four treatments. Proteins are involved in functionssuch asthe regulation of metabolism, stress response, signal transduction, regulation of gene expression, and cell death. One of the clusters (cluster 1), containing 59 proteins, revealed a few processes in the enrichment analysis, but the proteins contained in it have functions such as control of cell division, apoptosis, and protection from redox stress. It is possible to observe, in general, treatment with B[α]P increased the abundance of some proteins, which was partially reversed in group B[α]P + NR. On the other hand, the NR treatment decreased the abundance of proteins contained in this cluster. Another cluster (cluster 4) showed 51 proteins of decreased abundance during exposure to B [α] P, which was partially reversed in group B[α]P + NR. The proteins in this cluster are involved in important stages of the glycolytic pathway, also in growth, adhesion, migration, and cell invasion. Although it has been described that exposure to NR can increase the efficiency of DNA repair, the results presented in this work indicate that the protective effect may be related to the modulation of the cell cycle or cell adehsion modifications


Subject(s)
Proteomics/classification , Tobacco Products/classification , Carcinogenesis , Neoplasms , Cells/classification , Analysis of Variance , Data Interpretation, Statistical , Cell Death , Niacinamide/agonists , Oxidative Stress , Lung Neoplasms/pathology
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