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Braz. j. biol ; 83: e253009, 2023. tab, graf
Article in English | LILACS, VETINDEX | ID: biblio-1339373


Abstract Today, global focus of research is to explore the solution of energy crisis and environmental pollution. Like other agricultural countries, bulk quantities of watermelon peels (WMP) are disposed-off in environment as waste in Pakistan and appropriate management of this waste is the need of hour to save environment from pollution. The work emphasizes the role of ethanologenic yeasts to utilize significant sugars present in WMP for low-cost bioethanol fermentation. Dilute hydrochloric acid hydrolysis of WMP was carried out on optimized conditions employing RSM (response surface methodology) following central composite design (CCD). This experimental design is based on optimization of ethanologenesis involving some key independent parameters such as WMP hydrolysate and synthetic media ratio (X1), incubation temperature (X2) and incubation temperature (X3) for maximal ethanol yield exploiting standard (Saccharomyces cerevisiae K7) as well as experimental (Metchnikowia cibodasensisY34) yeasts. The results revealed that maximal ethanol yields obtained from S. cerevisiae K7 was 0.36±0.02 g/g of reducing sugars whereas M. cibodasensisY34, yielded 0.40±0.01 g ethanol/g of reducing sugars. The yeast isolate M. cibodasensisY34 appeared as promising ethanologen and embodies prospective potential for fermentative valorization of WMP-to-bioethanol.

Resumo Hoje, o foco global da pesquisa é explorar a solução da crise energética e da poluição ambiental. Como em outros países agrícolas, grandes quantidades de cascas de melancia (WMP) são descartadas como resíduos no meio ambiente no Paquistão, mas a gestão adequada desses resíduos é a mais recente solução para salvar o meio ambiente da poluição. O trabalho enfatiza o papel das leveduras etanologênicas para utilizar açúcares significativos presentes no WMP para fermentação de bioetanol de baixo custo. A hidrólise de ácido clorídrico diluído de WMP foi realizada em condições otimizadas empregando RSM (metodologia de superfície de resposta) e seguindo o projeto de composto central (CCD). Este projeto experimental é baseado na otimização da etanologenesis envolvendo alguns parâmetros independentes importantes, como hidrolisado de WMP e razão de meio sintético (X1), temperatura de incubação (X2) e temperatura de incubação (X3) para rendimento máximo de etanol explorando o padrão (Saccharomyces cerevisiae K7) também como leveduras experimentais (Metchnikowia cibodasensis Y34). Os resultados revelaram que os rendimentos máximos de etanol obtidos a partir de S. cerevisiae K7 foi de 0,36 ± 0,02 g / g de açúcares redutores, enquanto M. cibodasensis Y34 rendeu 0,40 ± 0,01 g de etanol / g de açúcares redutores. O isolado de levedura M. cibodasensis Y34 apareceu como um etanologeno promissor e incorpora um potencial prospectivo para a valorização fermentativa de WMP em bioetanol.

Cucurbitaceae , Ethanol , Saccharomyces cerevisiae , Water , Biotransformation , Prospective Studies , Fermentation
Article in Chinese | WPRIM | ID: wpr-928205


Liposomes with precisely controlled composition are usually used as membrane model systems to investigate the fundamental interactions of membrane components under well-defined conditions. Hydration method is the most common method for liposome formation which is found to be influenced by composition of the medium. In this paper, the effects of small alcohol (ethanol) on the hydration of lipid molecules and the formation of liposomes were investigated, as well as its coexistence with sodium chloride. It was found that ethanol showed the opposite effect to that of sodium chloride on the hydration of lipid molecules and the formation of liposomes. The presence of ethanol promoted the formation of liposomes within a certain range of ethanol content, but that of sodium chloride suppressed the liposome formation. By investigating the fluorescence intensity and continuity of the swelled membranes as a function of contents of ethanol and sodium chloride, it was found that sodium chloride and ethanol showed the additive effect on the hydration of lipid molecules when they coexisted in the medium. The results may provide some reference for the efficient preparation of liposomes.

Ethanol/pharmacology , Lipids , Liposomes
Article in Chinese | WPRIM | ID: wpr-928184


This study employed Box-Behnken design combined with flux attenuation to explore the nanofiltration conditions for separation of alcohol precipitation liquid during the preparation of Reduning Injection and discussed the applicability of nanofiltration in the separation of the liquid with high-concentration ethanol. The effects of nanofiltration molecular weight cut-off(MWCO) and pH on the rejection of chlorogenic acid, 3,5-dicaffeoylquinic acid, and 4,5-dicaffeoylquinic acid were consistent with the principles of pore size sieving and charge effect, respectively. The rejection of the three phenolic acids was reduced by concentration polarization effect caused by trans-membrane pressure(TMP). The swelling of membrane surface decreased the pore size and membrane flux for effective separation. Chlorogenic acid and 4,5-dicaffeoylquinic acid were more sensitive to pH and ethanol concentration than 3,5-dicaffeoylquinic acid. A certain correlation existed between the compound structure and the separation factors of nanofiltration, and the separation rules were associated with the comprehensive effect of charge effect, pore size sieving, concentration polarization, steric hindrance and so on.

Chlorogenic Acid , Drugs, Chinese Herbal/chemistry , Ethanol , Injections
Article in Chinese | WPRIM | ID: wpr-927996


In this study, the toxicological/pharmacological research method of "quantity-weight-evidence" network was first proposed and practiced to supplement the existing methodology of network toxicology. We transformed the traditional qualitative network into a quantitative network in this study by attributing weights to toxic component content and target frequency, which improved the reliability of data and provided a research idea for the systematic safety evaluation and toxicological research of Chinese medicinal herbs. Firstly, 50% ethanol extract of Dysosma versipellis(DV) was administrated to rats via gavage and the potential hepatotoxic components were identified by serum pharmacochemistry. Then, the component targets were obtained from SwissTargetPrediction, PharmMapper and other online databases, and the target weights were given according to the relative content of components and target fishing frequency. Meanwhile, the targets of hepatotoxicity were predicted from online databases such as Comparative Toxicology Database(CTD) and GeneCards. Subsequently, protein-protein interaction analysis and KEGG pathway enrichment were performed with the STRING database. Finally, the quantitative network of "toxic components-weighted targets-pathways" was constructed. Eleven potential toxic compounds were predicted, including podophyllotoxin, podophyllotoxone, deoxypodophyllotoxin, and 6-methoxypodophyllotoxin. A total of 106 hepatotoxic targets and 65 weighted targets(e.g., Cdk2, Egfr, and Cyp2 c9) were identified. The results of Kyoto Encyclopedia of Genes and Genomes(KEGG) pathway enrichment showed that these targets could act on PI3 K-AKT, MAPK, and Ras signaling pathways to play a role in inflammatory response and oxidative stress. However, traditional network toxicology showed that 51 targets such as AKT1, Alb, and Stat3 may lead to hepatotoxicity by mediating inflammation and cell proliferation. In conclusion, we proposed "quantity-weight-evidence" network toxicology in this study and used it to study the mechanism of DV-induced hepatotoxicity in rats. This study confirms the feasibility of this new methodology in toxicological evaluation and further improves the systematic evaluation of the safety of Chinese medicinal herbs.

Animals , Chemical and Drug Induced Liver Injury/etiology , Drugs, Chinese Herbal/toxicity , Ethanol , Medicine, Chinese Traditional , Molecular Docking Simulation , Rats , Reproducibility of Results
Rev. Esc. Enferm. USP ; 56: e20210402, 2022. graf
Article in English, Portuguese | LILACS, BDENF | ID: biblio-1365407


ABSTRACT Objective: To describe and discuss the participation of adolescents from a quilombola community in the transformation of the comic "Possible Story" ("Uma História Possível"), from the Comic on alcohol, into an educational game. Method: Implementation of the creative and sensitive method of art-based research, with adolescents from a quilombola community in the state of Espírito Santo, for the development of a board game. Results: The democratic and interactive space favored the problematization of images and narratives about alcohol consumption mediated in the comic book. The group selected scenes, reordered the story, devised questions and riddles, formulated true and false assertions in a 17-card composition of the board game. The potential of the game as a content mediating tool to promote learning, reinforcement, and fixation of scientific content was evaluated. Conclusion: The active and dynamic participation of adolescents took place from conception to evaluation of the board game, encouraging them to reflect on a community context of cultural permissiveness of alcohol use.

RESUMEN Objetivo: Describir y discutir la participación de adolescentes de una comunidad quilombola en la transformación de cómics de "Una Historia Posible" (Uma História Possível), del Almanaque sobre alcohol en juego educativo. Método: Implementación del método creativo y sensible de investigación basada en arte, con adolescentes de una comunidad quilombola de Espírito Santo, Brasil, en el desarrollo de un juego de mesa. Resultados: El espacio democrático e interactivo favoreció la problematización de imágenes y narrativas sobre el consumo de alcohol mediado en los cómics. El grupo seleccionó escenas, reordenó la historia, elaboró preguntas y charadas, formuló aserciones de mito o verdad en una composición de 17 fichas del juego de mesa. Se evaluó el potencial del juego como herramienta mediadora de contenido para promover el aprendizaje, el repaso y la memorización de contenidos científicos. Conclusión: La participación activa y dinámica de adolescentes ocurrió desde la concepción hasta la evaluación del juego de mesa, estimulándolos a la reflexión sobre un contexto comunitario de permisividad cultural de uso del alcohol.

RESUMO Objetivo: Descrever e discutir a participação de adolescentes de uma comunidade quilombola na transformação de quadrinhos de "Uma História Possível", do Almanaque sobre o álcool, em jogo educativo. Método: Implementação do método criativo e sensível de pesquisa baseada em arte, com adolescentes de uma comunidade quilombola do Espírito Santo, no desenvolvimento de um jogo de tabuleiro. Resultados: O espaço democrático e interativo favoreceu a problematização de imagens e narrativas sobre o consumo de álcool mediadas na história em quadrinhos. O grupo selecionou cenas, reordenou a história, elaborou perguntas e charadas, formulou asserções de verdadeiro e falso numa S composição de 17 cartas do jogo de tabuleiro. Avaliou-se o potencial do jogo como ferramenta mediadora de conteúdo para promover a aprendizagem, o reforço e fixação de conteúdos científicos. Conclusão: A participação ativa e dinâmica de adolescentes deu-se desde a concepção até a avaliação do jogo de tabuleiro, estimulando-os à reflexão sobre um contexto comunitário de permissividade cultural de uso do álcool.

Blacks , Ethanol , Educational and Promotional Materials , Pediatric Nursing , Adolescent
Acta toxicol. argent ; 29(2): 1-10, dic. 2021. graf
Article in Spanish | LILACS | ID: biblio-1364280


Resumen El consumo crónico de alcohol es un problema de salud mundial que afecta particularmente a la población femenina. Sin embargo, los efectos de la ingesta semicrónica en cantidades moderadas a bajas en el ovario y el oocito son poco conocidos. En un modelo murino, se administró etanol al 10% en agua de bebida (hembras tratadas) o agua (hembras control) por 15 días, y luego de la superovulación o no (ovulación espontánea), se analizó el ciclo estral y la calidad ovárico-gamética. En las hembras tratadas, la frecuencia y duración del diestro aumentó, y las frecuencias de folículos y cuerpos lúteos disminuyeron vs hembras controles, valores que se restauraron luego de la superovulación. Sin embargo, en las hembras tratadas, la tasa de proliferación celular folicular y el desbalance de la expresión ovárica de VEGF (factor de crecimiento endotelial) persistieron luego de la superovulación. El número de ovocitos ovulados con metafase II anormal, fragmentados y activados partenogenéticamente fue mayor en las hembras tratadas respecto las controles. En conclusión, el consumo semicrónico moderado de alcohol produce anestro, ciclo estral irregular, foliculogénesis deficiente y anomalías núcleo-citoplasmáticas en los oocitos ovulados. Estas alteraciones podrían constituirse en un factor etiológico de pérdida gestacional temprana y desarrollo embrionario anormal luego del consumo de alcohol.

Abstract Chronic alcohol consumption is a global health problem that particularly affects the female population. However, the ef-fects of semi-chronic ethanol intake in low-moderate amounts on the ovary and oocyte are poorly understood. In a mouse model, 10% ethanol was administered in drinking water (treated females) or water (control females) for 15 days, and after superovulation or not (spontaneous ovulation), the estrous cycle and ovarian-gametic quality were analyzed. In treated females, the frequency and duration of the diestrus increased, and the frequencies of follicles and corpus luteum decreased vs control females, values that restored after superovulation. However, in treated females, the follicular cell proliferation rate and the imbalance in ovarian expression of VEGF (endothelial growth factor) persisted after superovulation. The number of ovulated oocytes with abnormal metaphase II, fragmented and parthenogenetically activated was higher in treated females than in control ones. In conclusion, moderate semi-chronic alcohol consumption produces anestrum, irregular estrous cycle, poor folliculogenesis, and nuclear-cytoplasmic abnormalities in ovulated oocytes. These alterations could constitute an etiological factor of early gestational loss and abnormal embryonic development after alcohol consumption.

Humans , Animals , Female , Mice , Oocytes/drug effects , Alcohol Drinking/adverse effects , Ethanol/adverse effects , Ovarian Follicle/drug effects , Ovary/cytology , Ovary/drug effects , Oviducts/cytology , Oviducts/drug effects , Ovulation/drug effects , Models, Animal , Estrous Cycle/drug effects , Cell Proliferation , Germ Cells/cytology , Germ Cells/drug effects , Ovarian Follicle/cytology
Braz. j. oral sci ; 20: e211194, jan.-dez. 2021. ilus
Article in English | LILACS, BBO | ID: biblio-1253930


Aim: The aim of this study was to evaluate the effect of ethanol-conditioned dentin on endodontic sealer penetration into dentinal tubules by confocal laser scanning microscopy (CLSM). Methods: Forty human maxillary anterior teeth were instrumented and divided into four groups (n = 10) according to the drying methods: 1) wet: vacuum only, 2) paper points: vacuum + absorbent paper points, (3) 70% ethanol: 70% ethanol (1 min) + vacuum + absorbent paper points, and (4) 100% ethanol: 100% ethanol (1 min) + vacuum + absorbent paper points. All root canals were filled with resin-based endodontic sealer. Four sections from each third (cervical, middle, and apical) were examined by CLSM. Root canal wall perimeter infiltrated by sealer, maximum depth of sealer penetration, percentage of penetrated area, and fluorescence intensity of rhodamine B were measured. Statistical analysis was performed by analysis of variance and Tukey's tests (α = 0.05). Results: No statistical difference was found when percentage of root canal wall coverage infiltrated by sealer were compared. The groups in which ethanol solutions were used presented greater depth of sealer penetration, higher percentage of penetrated area, and higher fluorescence intensity of rhodamine B (p< 0.05) when compared with the wet and paper point groups. Overall, 100% ethanol produced better results than 70% ethanol, except for rhodamine B intensity (cervical third). In addition, the absorbent paper points drying method behaved better than did vacuum only group, except for rhodamine B intensity (apical third). Conclusion: Ethanol-conditioned dentin improved the penetration of resin-based sealer into dentinal tubules, especially at the concentration of 100%

Humans , Wettability , Microscopy, Confocal , Resin Cements , Dentin , Ethanol , Endodontics
Bol. latinoam. Caribe plantas med. aromát ; 20(6): 660-671, nov. 2021. tab, ilus
Article in English | LILACS | ID: biblio-1369981


Search for safe antioxidants and novel nutraceuticals urged to evaluate the antioxidant, anti-acetylcholine esterase and anti-lipoxygenase activity of various leaf extracts of Conocarpus lancifolius. Extraction was optimized from freeze dried plant extracts quenched with liquid nitrogen using water, ethanol, methanol, hexane, ethyl acetate and chloroform. Maximum extract yield, total phenolic contents and total flavonoid contents were obtained in case of ethanolic extraction. The highest 2,2-diphenyl-1-picrylhydrazylradical scavenging in terms of IC50 value of 55.26 µg/mL was observed for ethanolic leaf extract. The acetylcholine esterase and lipoxygenase inhibitory activities (IC50) were also observed for ethanolic extract. These findings for ethanolic extract were statistically significant when compared with other extracts (ρ<0.05). The haemolytic % values indicated that all extracts were associated with very low or negligible toxicity. The epicatechin, isorhamnetin, rutin, scopoleptin, skimmianine, quercetin-3-O-α-rhamnoside, quercetin-3-O-ß-glucoside, cornoside, creatinine, choline, pyruvic acid, α-hydroxybutyric acid, phyllanthin and hypophyllanthin were identified as major functional metabolites in ethanolic leaf extract of C. lancifoliusby 1H-NMR. The identified metabolites were probably responsible for the pharmacological properties of C.lancifolius. The findings may be utilized as pharmacological leads for drug development and food fortification.

Se insta a la búsqueda de antioxidantes seguros y nuevos nutracéuticos para evaluar la actividad antioxidante, anti-acetilcolina esterasa y anti-lipoxigenasa de varios extractos de hojas de Conocarpus lancifolius. La extracción se optimizó a partir de extractos de plantas liofilizados enfriados con nitrógeno líquido usando agua, etanol, metanol, hexano, acetato de etilo y cloroformo. En el caso de extracción etanólica se obtuvo el rendimiento máximo de extracto, el contenido de fenoles totales y el contenido de flavonoides totales. La mayor eliminación de radicales 2,2-difenil-1-picrilhidrazilo en términos de valor de CI50 de 55,26 µg/mL se observó para el extracto de hoja etanólico. También se observaron las actividades inhibidoras de la acetilcolina esterasa y lipoxigenasa (CI50) para el extracto etanólico. Estos hallazgos para el extracto etanólico fueron estadísticamente significativos en comparación con otros extractos (ρ<0.05). Los valores del % hemolítico indicaron que todos los extractos estaban asociados con una toxicidad muy baja o insignificante. Se identificaron la epicatequina, isorhamnetina, rutina, escopoleptina, skimmianina, quercetina-3-O-α-ramnosido, quercetina-3-O-ß-glucósido, cornosido, creatinina, colina, ácido pirúvico, ácido α-hidroxibutírico, filantrina e hipofillantina. como metabolitos funcionales principales en el extracto etanólico de hojas de C. lancifoliuspor 1H-NMR. Los metabolitos identificados probablemente fueron responsables de las propiedades farmacológicas de C. lancifolius. Los hallazgos pueden utilizarse como pistas farmacológicas para el desarrollo de fármacos y la fortificación de alimentos.

Plant Extracts/pharmacology , Combretaceae/chemistry , Antioxidants/pharmacology , Phenols/analysis , Flavonoids/analysis , In Vitro Techniques , Plant Extracts/chemistry , Cholinesterase Inhibitors/pharmacology , Cholinesterase Inhibitors/chemistry , Free Radical Scavengers , Lipoxygenase Inhibitors/pharmacology , Lipoxygenase Inhibitors/chemistry , Ethanol , Antioxidants/chemistry
Arch. endocrinol. metab. (Online) ; 65(3): 322-327, May-June 2021. tab, graf
Article in English | LILACS | ID: biblio-1285159


ABSTRACT Objective: Percutaneous ethanol injection (PEI) is an alternative to surgery for the treatment of thyroid nodules (TNs). However, size reductions of treated (TTNs) and untreated TN (UTNs) have not been compared. Volumetric reductions in TTNs with PEI were evaluated by comparing TTNs and UTNs in the same patient, and independent variables predicting good post-PEI outcomes were analyzed. Materials and methods: Overall, 282 patients with multinodular goiters were selected. Two nodules located in different lobes were compared for common disease behaviors. Overall, 150 nodules were selected from 75 patients (6 M: 69 F) with a mean age of 50.1 ± 17.4 years. This prospective nonrandomized intervention study prioritized treating TNs of greater volume or single hyperfunctioning TNs. A single observer experienced in PEI and an ultrasound specialist performed the interventions. Results and discussion: TTNs (mean volume: 14.8 ± 16.2 mL) were reduced by 72.6 ± 27.3% of their initial volume, while UTNs increased by a mean of 365.7 ± 1.403.8% (p < 0.00001). The patients underwent a mean of 4.0 ± 3.1 outpatient PEI sessions without relevant complications. Logistic regression analysis showed that the magnitude of the PEI induced reduction was associated with the number of treatment sessions (p = 0.03, CI [1.1-38.2]) and not with ultrasonographic characteristics of the nodules. Each PEI session increased the rate of TN reduction by a factor of 6.7. Conclusions: PEI is a well-tolerated outpatient procedure that effectively reduces the volume of TNs and is noticeably superior to conservative treatment for all ultrasonographic classifications.

Humans , Adult , Aged , Thyroid Nodule/drug therapy , Thyroid Nodule/diagnostic imaging , Prospective Studies , Ultrasonography , Treatment Outcome , Ethanol , Middle Aged
Rev. inf. cient ; 100(3): e3455, 2021. tab
Article in Spanish | LILACS, CUMED | ID: biblio-1289633


RESUMEN Introducción: La cirrosis hepática es una de las enfermedades más frecuentes en nuestro país, y la presencia de infecciones y su descompensación constituyen motivos de ingreso hospitalario en estos pacientes. Objetivo: Identificar las enfermedades asociadas al cuidado sanitario más frecuentes en pacientes cirróticos ingresados. Método: Se realizó un estudio de cohorte en 90 pacientes con diagnóstico de cirrosis hepática, ingresados en el Hospital General Provincial "Carlos Manuel de Céspedes" de provincia Granma, en el período comprendido desde enero de 2017 hasta septiembre de 2020. La población estuvo conformada por la totalidad de pacientes mayores de 18 años y de ambos sexos. Los datos se recogieron de las historias clínicas hospitalarias. En el análisis estadístico se empleó el estadígrafo Chi-cuadrado obtenido a partir de tablas de contingencia, y para medir la fuerza de la misma los riesgos relativos (RR), se utilizó un nivel de significación (valor p) menor de 0,05. Resultados: La mayoría de los pacientes fueron del sexo masculino, descompensados con Child-Pugh (B), el alcohol como la etiología más frecuente de la cirrosis, a los que se les realizaron cuidados sanitarios como la colocación de sonda vesical, abordaje venoso central y periférico, así como intubación endotraqueal. Las principales infecciones asociadas al cuidado sanitario observadas en estos pacientes fueron la flebitis, bacteriemia, la infección del tracto urinario y la neumonía. Conclusiones: Las infecciones asociadas al cuidado sanitario según orden de frecuencia fueron la flebitis, la bacteriemia, la pielonefritis, neumonía y cistitis.

ABSTRACT Introduction: Liver cirrhosis has been confirmed as one of the most common diseases in Cuba. Infection and decompensated cirrhosis constitute the cause of hospitalization. Objective: To identify the most frequent health care-associated diseases in hospitalized cirrhotic patients. Method: A cohort study involving 90 hospitalized patients with cirrhosis was conducted at the Hospital General Provincial "Carlos Manuel de Céspedes" in Granma, from January 2017 through September 2020. The total patients underwent study were over 18 years old and both sex. Data were collected from patients´ hospitalization history. Chi-square test was utilized for statistical analysis and to measure its power (the relative risk), a P-value less than 0.05 was used. Results: Most patients were male, decompensated with Child-Pugh class B. All hospitalized patients who underwent health care such as bladder catheter placement, central and peripheral venous approach, as well as endotracheal intubation, alcohol was considered the major etiological factor cause of cirrhosis. The main healthcare-associated infections observed in these patients were phlebitis, bacteremia, urinary tract infection and pneumonia. Conclusions: The healthcare-associated infections in order of frequency were phlebitis, bacteremia, pyelonephritis, pneumonia and cystitis.

RESUMO Introdução: A cirrose hepática é uma das doenças mais frequentes em nosso país, e a presença de infecções e sua descompensação constituem motivos de internação nesses pacientes. Objetivo: Identificar as doenças associadas aos cuidados de saúde mais frequentes em pacientes cirróticos hospitalizados. Método: Foi realizado um estudo de coorte em 90 pacientes com diagnóstico de cirrose hepática, internados no Hospital Geral Provincial "Carlos Manuel de Céspedes" da província de Granma, no período de janeiro de 2017 a setembro de 2020. A população foi constituída por para todos os pacientes com mais de 18 anos de idade e de ambos os sexos. Os dados foram coletados em prontuários hospitalares. Na análise estatística, foi utilizada a estatística Qui-quadrado obtida em tabelas de contingência e, para medir a força dos riscos relativos (RR), foi utilizado um nível de significância (p-valor) menor que 0,05. Resultados: A maioria dos pacientes era do sexo masculino, descompensados com Child-Pugh (B), sendo o álcool a etiologia mais frequente da cirrose, que realizaram cuidados de saúde como colocação de cateter vesical, abordagem venosa central e periférica, além de intubação endotraqueal. As principais infecções associadas aos cuidados de saúde observadas nestes pacientes foram flebite, bacteremia, infecção do trato urinário e pneumonia. Conclusões: As infecções associadas aos cuidados de saúde em ordem de frequência foram flebite, bacteremia, pielonefrite, pneumonia e cistite.

Humans , Male , Female , Middle Aged , Heart Disease Risk Factors , Infections , Liver Cirrhosis/complications , Phlebitis , Pneumonia , Pyelonephritis , Prospective Studies , Bacteremia , Cystitis , Ethanol
Rev. colomb. gastroenterol ; 36(supl.1): 98-101, abr. 2021. graf
Article in Spanish | LILACS | ID: biblio-1251555


Resumen Se presenta el caso de un paciente con antecedente de consumo crónico de alcohol, que consultó por dolor abdominal inespecífico, fiebre intermitente no cuantificada y pérdida de peso, con posterior aumento del perímetro abdominal. Se encontró ascitis y hallazgos en imágenes que sugerían cirrosis. El estudio del líquido ascítico fue no hipertensivo con predominio de linfocitos y niveles de adenosina-desaminasa (ADA) elevados. La ecografía y tomografía de abdomen mostraron el engrosamiento del peritoneo y la biopsia peritoneal por laparoscopia fue compatible con enfermedad granulomatosa, con reacción en cadena de la polimerasa (PCR) positiva para Mycobacterium tuberculosis en un paciente sin otras causas de inmunosupresión. Este caso muestra la necesidad de mantener una alta sospecha clínica de TB en patologías abdominales con clínica inespecífica, aun en pacientes sin inmunocompromiso claro.

Abstract This is the case of a patient with a history of chronic alcohol consumption, who consulted for nonspecific abdominal pain, intermittent fever, and weight loss, with subsequent increase in the abdominal perimeter. Ascites and imaging findings suggestive of cirrhosis were found. The study of ascitic fluid was non-hypertensive with a predominance of lymphocytes and elevated adenosine deaminase (ADA) levels. Ultrasound and abdominal tomography showed peritoneal thickening. Laparoscopic peritoneal biopsy was compatible with granulomatous disease, with positive PCR for Mycobacterium tuberculosis in a patient with no other causes of immunosuppression. This report shows the importance of keeping a high index of suspicion for TB in patients with abdominal pathology, even in those without evident inmunocompromise.

Humans , Male , Middle Aged , Peritoneum , Ascites , Tuberculosis , Alcohol Drinking , Ethanol
Rev. colomb. gastroenterol ; 36(supl.1): 19-25, abr. 2021. graf
Article in Spanish | LILACS | ID: biblio-1251541


Resumen La pancreatitis del surco (PS) o "groove pancreatitis", es una forma de pancreatitis crónica infrecuente; nombrada así por su localización en el surco pancreatoduodenal. Suele predominar en varones con antecedente de ingesta de alcohol. Es de gran importancia realizar diagnóstico diferencial con cáncer de páncreas, dada su ubicación. Los avances en los métodos diagnósticos, como la ultrasonografía endoscópica, han permitido un abordaje más certero, sin embargo, no se cuenta con guías de manejo y las aproximaciones terapéuticas siguen siendo basadas en patologías similares.

Abstract Groove pancreatitis (GP) is a rare form of chronic pancreatitis located in the pancreaticoduodenal groove, hence its name. It is predominant in males with a history of alcohol intake. Making a differential diagnosis between this condition and pancreatic cancer is highly relevant given its location. Advances in diagnostic methods, such as endoscopic ultrasonography, have allowed a more accurate approach. However, no management guidelines are available and therapeutic approaches are still based on similar pathologies.

Humans , Male , Adult , Pancreatitis, Chronic , Similar , Diagnosis, Differential , Ethanol
Med. U.P.B ; 40(1): 77-81, 03/03/2021. Ilus
Article in Spanish | LILACS, COLNAL | ID: biblio-1177502


Los diferentes reportes de consumo de sustancias evidencian cómo el consumo de alcohol afecta diferentes órganos y sistemas; según el tiempo de presentación hay riesgos agudos y crónicos. Dentro de las complicaciones agudas gastrointestinales asociadas al consumo de alcohol está el síndrome de Boerhaave consistente en una ruptura esofágica espontánea. Es importante identificar este síndrome porque se relaciona con alta mortalidad debido a la amplia gama de signos y síntomas que produce, como vómito, disnea, taquipnea, taquicardia y dolor esternal, que pueden generar confusión con otras enfermedades como el tromboembolismo pulmonar. El objetivo de este reporte es pre-sentar el primer caso clínico en Colombia de un paciente con síndrome de Boerhaave como complicación del consumo de alcohol, ya que es importante que el personal de salud reconozca los factores de riesgo que lo desencadenan.

Numerous reports of substance use show how alcohol consumption affects different organs and systems; related risks can be acute and chronic, depending on the time of presentation. Among the acute gastrointestinal complications associated with alcohol consumption is Boerhaave syndrome, which consists of a spontaneous esophageal rupture. It is important to identify this pathology because it is associated with high mortality due to the wide range of signs and symptoms that it produces such as vomiting, dyspnea, tachypnea, tachycardia, and sternal pain, which can lead to confusion with other diseases like pulmonary thromboembolism and may therefore delay proper and timely diagnostic. The objective of this report is to present the first clinical case reported in Colombia of a patient who suffered from Boerhaave syndrome secondary to chronic alcohol consumption and to sensitize the health personnel about the importance of recognizing alcohol consumption as a risk factor for this complication.

Os diferentes relatos de uso de substâncias mostram como o consumo de álcool afeta diferentes órgãos e sistemas; dependendo da época de apresentação, existem riscos agudos e crônicos. Entre as complicações gastrointestinais agudas associadas ao con-sumo de álcool está a síndrome de Boerhaave, que consiste em uma ruptura esofágica espontânea. É importante identificar essa síndrome, pois está associada a alta mortalidade devido à ampla gama de sinais e sintomas que produz, como vômitos, dispneia, taquipneia, taquicardia e dor esternal, que podem levar à confusão com outras doenças, como tromboembolismo pulmonar. O objetivo deste relatório é apresentar o primeiro caso clínico na Colômbia de um paciente com síndrome de Boerhaave como uma complicação do consumo de álcool, pois é importante que o pessoal de saúde reconheça os fatores de risco que a desencadeiam.

Humans , Alcohol Drinking , Pain , Pulmonary Embolism , Rupture , Tachycardia , Vomiting , Confusion , Ethanol
Article in English | WPRIM | ID: wpr-922259


: To investigate the protective effect of (FD) against ethanol-induced gastric ulcer and its mechanism. : Human gastric epithelial GES-1 cells were divided into normal control group, model control group, FD 95% alcohol extract group, FD 50% alcohol extract group and FD decoction extract group. Gastric ulcer was induced by treatment with 1% ethanol in GES-1 cells. The cell proliferation was detected with MTT method in each group. Sixty SD rats were randomly divided into normal control group, model control group, ranitidine group and low-dose, medium-dose, high-dose FD 95% alcohol extract groups (150, 300, 600 mg/kg). The corresponding drugs were administrated by gavage for The gastric ulcer model was induced by intragastric administration of anhydrous ethanol. The gastric ulcer area and ulcer inhibition rate of rats were measured in each group; the degree of gastricmucosal damage was observed by scanning electron microscopy; the levels of tumor necrosis factor (TNF)-α, interleukin (IL)-6, IL-1β in serum and the content of malondialdehyde (MDA), superoxide dismutase (SOD), glutathione (GSH), catalase (CAT) in gastric tissues were detected by ELISA method. : 95% alcohol extract of FD had the strongest protective effect on proliferation of GES-1 cells. In animal experiments, compared with the normal control group, a large area of ulcers appeared on the gastric mucosa in the model control group, while the ulcer areas of the FD groups and ranitidine group were significantly smaller than that of the model control group (all <0.05). Compared with the model control group, FD groups and ranitidine group significantly reduced the levels of TNF-α, IL-1β, IL-6 in serum and the MDA content in the gastric tissues, and increased the activity of SOD, CAT and GSH in gastric tissues (all <0.05). : The 95% alcohol extract of FD can reduce the levels of TNF-α, IL-1β and IL-6 in serum and the content of MDA in gastric tissues, and increase the activity of SOD, CAT and GSH in gastric tissues to achieve the protective effect against gastric ulcer.

Animals , Ethanol/toxicity , Gastric Mucosa , Malondialdehyde , Rats , Rats, Sprague-Dawley , Stomach Ulcer/prevention & control , Superoxide Dismutase
Article in English | WPRIM | ID: wpr-922105


OBJECTIVE@#To explore the antitumor effects of ethanol extract from Ventilago leiocarpa Benth (EEVLB) on sarcoma 180 (S180) tumor-bearing mice and the potential mechanism.@*METHODS@#Sixty mice were randomly assigned to 6 groups according to a random number table: normal group, model group, 5-fluorouracil (5-FU) group (0.02 g·kg@*RESULTS@#EEVLB with different concentrations achieved inhibition of tumor growth in vivo, wherein the high-dose group showed the most significant reduction in tumor weight and increased apoptosis of tumor cells (P<0.05). In addition, both net weight gain and spleen index of mice showed uptrend in EEVLB treatment groups (P<0.05). Besides, serum levels of IL-2 and IL-6, percentages of CD3@*CONCLUSIONS@#EEVLB exhibits promising antitumor activity in vivo. This effect might be due to activation of apoptotic signaling pathway, increase of cytokine levels and enhancement of immune function in tumor-bearing mice.

Animals , Cell Line, Tumor , Ethanol , Mice , Plant Extracts/therapeutic use , Rhamnaceae , Sarcoma 180/drug therapy
Article in Chinese | WPRIM | ID: wpr-921753


The present study explored the potential mechanism of Jingfang Granules in relieving alcohol and protecting liver by network pharmacology and molecular docking and verified the effects and related pathways by animal experiments. The active components of Jingfang Granules were retrieved from Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP). Targets of drugs and diseases were obtained from PubChem, Swiss Target Prediction and CTD. The common targets were uploaded to STRING to plot the protein-protein interaction(PPI) network. The core targets were screened out and the target organs were identified by Bio GPS and Metascape, followed by Gene Ontology(GO) analysis and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analysis of common targets. The acute drunk mouse model was established and the effects of Jingfang Granules on serum ethanol level and the expression of proteins related to the phosphatidylinositol 3-kinase(PI3 K)/protein kinase B(Akt) signaling pathway in the liver tissue of mice were observed. A total of 187 active components of Jingfang Granules were obtained, including 47 common targets with alcoholic liver injury. GO enrichment analysis and KEGG pathway analysis showed that Jingfang Granules might play the role of relieving alcohol and protecting liver through the PI3 K-Akt signaling pathway. The drug-component-target and component-target-pathway networks revealed that the important active components of Jingfang Granules in relieving alcohol and protecting liver included quercetin, 5-O-methylvisamminol, glyasperin M, glyasperin B and hederagenin. Molecular docking showed that the active components had a good affinity with AKT1, EGFR, ESR1 and PTGS2. Experimental results showed that Jingfang Granules(15 and 10. 5 g·kg-1) could significantly reduce the content of serum ethanol in mice and up-regulate the protein expression ratios of p-PI3 K/PI3 K and p-Akt/Akt in the liver tissue. Jingfang Granules could relieve alcohol and protect liver through multi-component and multitarget, and the mechanism may be related to the activation of the PI3 K-Akt signaling pathway.

Animals , Computational Biology , Drugs, Chinese Herbal/pharmacology , Ethanol , Liver , Medicine, Chinese Traditional , Mice , Molecular Docking Simulation , Network Pharmacology , Technology
Article in Chinese | WPRIM | ID: wpr-921677


The present study investigated the chemical constituents of Caesalpinia decapetala in the Fabaceae family. The chemical constituents were isolated and purified by chromatographies with silica gel, RP-C_(18), Sephadex LH-20, and preparative HPLC, and their structures were determined based on the spectroscopic data and physicochemical properties, as well as relevant references. Three pairs of new dibenzoxocin derivatives were isolated from 70% ethanol extract of C. decapetala and identified as protosappanoside A(1 a), isoprotosappanoside A(1 b), protosappanoside B(2 a), isoprotosappanoside B(2 b), protosappanoside C(3 a), and isoprotosappanoside C(3 b), respectively.

Caesalpinia , Chromatography, High Pressure Liquid , Ethanol , Molecular Structure , Plant Extracts
Article in Chinese | WPRIM | ID: wpr-888187


This paper aimed to explore the anti-inflammatory effect of ethanol extract from Saposhnikoviae Radix in a lipopolysaccharide(LPS)-induced inflammation mouse model and its regulation of TLR4/NF-κB signaling pathway. The ethanol extract from Saposhnikoviae Radix was separated and purified on the macroporous adsorption resin and its main chemical components were identified by UPLC-QE/MS. The identification results showed that the top ten components of ethanol extract from Saposhnikoviae Radix were mainly chromones and coumarins. A mouse model of inflammation induced by intraperitoneal injection of LPS was used to investigate the anti-inflammatory effects of ethanol extract from Saposhnikoviae Radix after intragastric administration for seven successive days. Mice in all groups except for the control group were treated with intraperitoneal injection of LPS(0.015 g·kg~(-1)) one hour after the last administration, and twelve hours later, the blood was sampled and separated and the broncoalveolar lavage fluid(BALF) was collected. The levels of nitric oxide(NO), tumor necrosis factor-α(TNF-α), interleukin-6(IL-6), and interleukin-1β(IL-1β) in mouse serum and BALF were detected by ELISA. The harvested lung tissue was stained with hematoxylin-eosin(HE) for observing the pathological changes, followed by the detection of protein expression levels of related molecules in TLR4/NF-κB signaling pathway by Western blotting. The results showed that the ethanol extract from Saposhnikoviae Radix significantly ameliorated the pathological conditions in lung tissue of model mice, reversed the increase in NO, TNF-α, IL-6, and IL-1β levels of mouse serum and BALF, down-regulated the protein expression levels of Toll-like receptor 4(TLR4), myeloid differentiation factor(MyD88), and phosphorylated nuclear transcription factor κB-p65/nuclear transcription factor κB-p65(P-NF-κB p65/NF-κB p65), and up-regulated the NF-κB inhibitory protein α(IκBα). The ethanol extract from Saposhnikoviae Radix exhibited a good anti-inflammatory effect in the LPS-induced acute inflammation muse model, which might be related to the inhibition of the activation of TLR4/NF-κB inflammatory signaling pathway. Chromones and coumarins have been proved to be the active components for its anti-inflammatory effects.

Animals , Anti-Inflammatory Agents , Ethanol , Inflammation/drug therapy , Lipopolysaccharides/toxicity , Mice , NF-kappa B/genetics , Plant Extracts
Article in Chinese | WPRIM | ID: wpr-888115


As a common disease worldwide, alcoholic liver injury is caused by long-term or excessive intake of alcohol and triggers cell death due to alcohol metabolism and reactive oxygen species(ROS)-mediated cytotoxicity. Wangshi Baochi(WSBC) Pills have been widely adopted in clinical practice for evacuating stasis, resolving turbidity, clearing heat, tranquilizing mind, invigorating sto-mach, promoting digestion, purging fire and removing toxin. This study aimed to investigate the efficacy of WSBC Pills in dispelling the effect of alcohol and protecting against acute alcoholic liver/stomach injury in mice, and preliminarily investigate its possible mole-cular mechanism. The results found that the preventive treatment with WSBC Pills contributed to elevating the activity of alcohol dehydrogenase(ADH) and its expression in liver and shortening the time required for sobering up of mice with acute alcoholic liver injury. The staining of liver pathological sections as well as the detection of serum aspartate aminotransferase(AST) and alanine aminotransferase(ALT) and liver ROS levels revealed that WSBC Pills protected the liver by reducing serum AST and ALT. It suppressed oxidative stress-induced liver injury by lowering liver ROS and elevating superoxide dismutase(SOD), and the liver-protecting effect was superior to that of silibinin. Western blot assay confirmed that WSBC Pills inhibited the oxidative stress by up-regulating SOD1 and NAD(P)H: quinone oxidoreductase 1(NQO-1). In addition, WSBC Pills lowered the ROS level to protect against the acute alcoholic stomach injury in mice. The findings have suggested that WSBC Pills alleviated the acute alcoholic liver/stomach injury in mice by increasing ADH and resisting oxidative stress.

Animals , Chemical and Drug Induced Liver Injury , Ethanol , Liver/metabolism , Liver Diseases, Alcoholic , Mice , Oxidative Stress , Stomach
Article in Chinese | WPRIM | ID: wpr-879096


Prunella vulgaris(PV) is an edible and traditional medicinal herb which has a wide range application in fighting inflammation and oxidative stress, and protecting liver. Now it has been used to treat various types of liver diseases and has significant clinical efficacy. This study aims to investigate the effects of PV on ethanol-induced oxidative stress injury in rats and its metabolic mechanism. The rats were divided into control group, model group, PV group, and VC group. The liver protection of PV was identified by measuring pharmacological indexes such as antioxidant and anti-inflammatory activity. The metabolic mechanism of long-term ethanol exposure and the metabolic regulation mechanism of PV treatment were studied by LS-MS metabonomics. The pharmacological investigation indicated that ethanol could significantly decrease the contents of SOD, GSH-Px, CAT and other antioxidant enzymes in liver and increase the content of MDA. At the same time, PV could significantly reduce the contents of inflammatory factors(TNF-α, IL-6 and IL-1β) and liver function markers(ALT, AST, ALP) in serum. What's more, long-term ethanol exposure could significantly cause liver injury, while PV could protect liver. Metabolomics based on multiple statistical analyses showed that long-term ethanol exposure could cause significant metabolic disorder, and fatty acids, phospholipids, carnitines and sterols were the main biomarkers. Meanwhile, pathway analysis and enrichment analysis showed that the β oxidation of branched fatty acids was the main influencing pathway. Also, PV could improve metabolic disorder of liver injury induced by ethanol, and amino acids, fatty acids, and phospholi-pids were the main biomarkers in PV treatment. Metabolic pathway analysis showed that PV mainly regulated metabolic disorder of ethanol-induced liver injury through phenylalanine, tyrosine and tryptophan biosynthetic pathways. This study could provide a new perspective on the hepatoprotective effect of natural medicines, such as PV.

Animals , Antioxidants/metabolism , Ethanol/toxicity , Liver/metabolism , Metabolomics , Oxidative Stress , Prunella , Rats