Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 20 de 150
Filter
1.
Medicentro (Villa Clara) ; 27(2)jun. 2023.
Article in Spanish | LILACS | ID: biblio-1440532

ABSTRACT

La necrosis retinal aguda es una afección grave que amenaza la visión. Es frecuente en adultos, tanto inmunocompetentes como inmunocomprometidos. Se presentan dos pacientes, uno de 38 años, con antecedentes de salud anterior que acude a consulta con síntomas y signos de necrosis retinal aguda en el ojo izquierdo, la que fue diagnosticada luego; y otro de 48 años de edad con antecedentes de infección por herpes zóster, tres meses antes de los síntomas oculares, que concluyó con igual diagnóstico. No existió evolución satisfactoria, a pesar del tratamiento adecuado, lo que demostró que independientemente de datos estadísticos y estudios realizados que demuestran lo infrecuente de esta enfermedad, se diagnosticaron dos casos en el periodo de un año, dato que nos exhorta al estudio y práctica de alternativas diagnósticas y terapéuticas para minimizar las consecuencias devastadoras de esta afección.


Acute retinal necrosis is a serious vision-threatening condition. It is common in both immunocompetent and immunocompromised adults. We present two male patients; one aged 38 years, with a previous health history who comes to consultation with symptoms and signs of acute retinal necrosis in his left eye, which was later diagnosed; and another one aged 48 years with a history of herpes zoster infection three months before the ocular symptoms, which concluded with the same diagnosis. Regardless of the statistical data and research carried out on this rare disease, there was no satisfactory evolution despite adequate treatment. Two cases were diagnosed in a period of one year, data that urges us to study and practice diagnostic and therapeutic alternatives to minimize the devastating consequences of this condition.


Subject(s)
Retinal Necrosis Syndrome, Acute , Herpesvirus 2, Human , Herpesvirus 1, Human , Vitreoretinopathy, Proliferative
2.
Med. lab ; 27(2): 97-109, 2023. Tabs, Grafs
Article in Spanish | LILACS | ID: biblio-1435401

ABSTRACT

Introducción. Las infecciones de transmisión sexual (ITS) son y seguirán siendo un serio problema de salud pública en todo el mundo según los datos de la OMS, con el agravante que la mayoría de los casos son asintomáticos y, además, no existe otro reservorio distinto al humano. El diagnóstico se puede realizar con pruebas tradicionales y moleculares, estas últimas incluyen la reacción en cadena de la polimerasa (PCR), de las cuales existen varios tipos, entre ellas, la PCR múltiple que tiene la capacidad de detectar ITS polimicrobianas a partir de una sola muestra. El objetivo de este estudio fue establecer cuáles fueron las infecciones de transmisión sexual más frecuentes en diferentes grupos de pacientes, así como determinar la utilidad del uso de la técnica de PCR múltiple en el diagnóstico de las ITS. Metodología. Se trata de un estudio observacional de corte transversal realizado entre los años 2021 y 2022 con pacientes que acudieron al servicio de diagnóstico del Laboratorio Clínico VID por sospecha de ITS. Las muestras recolectadas fueron evaluadas utilizando una prueba comercial basada en la técnica de PCR múltiple e hibridación. Las muestras procesadas fueron: orina e hisopados de endocérvix, uretra, recto, faringe y úlceras. Resultados. Se estudiaron 1.027 pacientes, de estos, 228 (22,2 %) fueron positivos para diferentes agentes de trasmisión sexual, distribuidos así: 50 (21,9 %) mujeres, 129 (56,6 %) hombres heterosexuales y 49 (21,5 %) hombres que tenían sexo con hombres (HSH). La edad promedio de las mujeres fue 30 años, y la de ambos grupos de hombres fue 36 años. Los microorganismos más frecuentemente identificados en mujeres fueron: C. trachomatis (A-K) en 28,6 %, seguido de virus herpes simplex tipo 2 (VHS-2) en 26,8 % y N. gonorrhoeae en 17,9 %. En hombres heterosexuales fueron C. trachomatis (A-K) en 37,5 %, N. gonorrhoeae en 21,5 % y VHS-2 en 18,7 %. En HSH fueron C. trachomatis (L1-L3) en 32,7 %, seguido de N. gonorrhoeae en 27,6 %, y de C. trachomatis (A-K) y VHS-2, ambos en 13,8 %. En 11 hombres heterosexuales, 8 HSH y en 6 mujeres, se identificó infección polimicrobiana. Conclusiones. C. trachomatis (A-K) fue el microorganismo más prevalente causante de ITS, seguido de N. gonorrhoeae en ambos grupos de hombres, y de VHS-2 en las mujeres, muy similar a lo reportado a nivel mundial. La prueba de PCR múltiple permite la detección de infecciones polimicrobianas comúnmente asociadas a ITS y el diagnóstico es preciso y confiable, incluso en pacientes asintomáticos


Sexually transmitted infections (STIs) are and will continue to be a serious public health problem throughout the world according to WHO data, with the aggravating factor that most cases are asymptomatic and, furthermore, there is no other reservoir other than humans. The diagnosis can be made with traditional and molecular tests, the latter include the polymerase chain reaction (PCR), of which there are several types, among them, multiplex PCR that has the capacity to detect polymicrobial STIs from a single sample. The objective of this study was to establish which were the most frequent sexually transmitted infections in different groups of patients, as well as to determine the usefulness of the multiplex PCR technique in the diagnosis of STIs. Methodology. This is an observational, cross-sectional study carried out between 2021 and 2022 with patients who attended the VID Clinical Laboratory for suspected STIs. The collected samples were evaluated using a commercial test based on the multiplex PCR technique and hybridization. The samples processed were: urine and swabs from endocervix, urethra, rectum, pharynx, and ulcers. Results. The study included 1,027 patients, of these, 228 (22.2%) were positive for different sexually transmitted agents, distributed as follows: 50 (21.9%) women, 129 (56.6%) heterosexual men and 49 (21.5%) men who had sex with men (MSM). The average age of the women was 30 years, and that of both groups of men was 36 years. The microorganisms most frequently identified in women were: C. trachomatis (A-K) in 28.6%, followed by herpes simplex virus type 2 (HSV-2) in 26.8% and N. gonorrhoeae in 17.9%. In heterosexual men they were C. trachomatis (A-K) in 37.5%, N. gonorrhoeae in 21.5% and HSV-2 in 18.7%. In MSM they were C. trachomatis (L1-L3) in 32.7%, followed by N. gonorrhoeae in 27.6%, and C. trachomatis (A-K) and HSV-2, both in 13.8%. Polymicrobial infection was identified in 11 heterosexual men, 8 MSM, and 6 women. Conclusions. C. trachomatis (A-K) was the most prevalent STI-causing microorganism, followed by N. gonorrhoeae in both groups of men, and HSV-2 in women, very similar to that reported worldwide. The multiplex PCR test allows the detection of polymicrobial infections commonly associated with STIs and the diagnosis is accurate and reliable, even in asymptomatic patients


Subject(s)
Humans , Polymerase Chain Reaction , Sexually Transmitted Diseases , Chlamydia trachomatis , Herpesvirus 2, Human , Molecular Diagnostic Techniques , Neisseria gonorrhoeae
3.
Rev. chil. infectol ; 39(2): 214-217, abr. 2022. ilus
Article in Spanish | LILACS | ID: biblio-1388344

ABSTRACT

Resumen Las cervicitis es una condición frecuente causada principalmente por agentes de transmisión sexual. Su presentación clínica varía desde cuadros asintomáticos hasta procesos inflamatorios extensos, que incluso asemejan un tumor maligno. Presentamos el caso de una adolescente que presentó úlceras genitales, síntomas generales y cérvix necrótico con aspecto tumoral. Los estudios de laboratorio confirmaron una co-infección por virus herpes simplex 2 (HSV-2) y Mycoplasma genitalium. El estudio histológico descartó una neo- plasia. Evolucionó favorablemente al tratamiento antimicrobiano, con recuperación progresiva del aspecto del cérvix. La cervicitis en raras ocasiones se presenta con compromiso necrótico. La co-infección por HSV-2 y M. genitalium, en este caso, pudo ser el determinante del daño cervical y la necrosis. Una evaluación acuciosa y estudio con exámenes diagnósticos de alta sensibilidad y especificidad permitieron hacer un diagnóstico y tratamiento adecuado.


Abstract Cervicitis is a frequent condition caused mainly by sexually trans- mitted agents. The clinical spectrum varies from absence of symptoms to extensive inflammatory processes that may simulate a malignant neoplasm. We present a clinical case of an adolescent with genital ulcers and systemic disease. Speculoscopy revealed a tumoral-looking cervix. Laboratory studies confirm infection with herpes simplex virus 2 (HSV-2) and Mycoplasma genitalium, together with a histological study that ruled out neoplasia. It progresses favorably to antimicrobial treatment, with recovery of the appearance of the cervix. Cervicitis rarely presents with necrotic involvement. Co-infection with HSV-2 and M. genitalium infection may have been the determinant of cervical damage and the necrotic appearance. A thorough evaluation and study with highly sensitive and specific diagnostic tests allowed an adequate diagnosis and treatment.


Subject(s)
Humans , Female , Adolescent , Uterine Cervicitis/complications , Uterine Cervicitis/diagnosis , Uterine Cervicitis/drug therapy , Mycoplasma genitalium , Coinfection , Mycoplasma Infections/complications , Mycoplasma Infections/diagnosis , Mycoplasma Infections/drug therapy , Herpesvirus 2, Human
4.
Rev. Soc. Bras. Med. Trop ; 54: e01272021, 2021. tab
Article in English | LILACS | ID: biblio-1347093

ABSTRACT

Abstract INTRODUCTION: Herpesviruses, enteroviruses, and arboviruses are important because of their clinical relevance and ability to cause meningitis, encephalitis, meningoencephalitis, and other diseases. The clinical virology associated with diagnostic technologies can reduce the morbidity and mortality of such neurological manifestations. Here we aimed to identify the genomes of agents that cause neurological syndromes in cerebrospinal fluid (CSF) samples from patients with suspected nervous system infections admitted to the University Hospital of the University of Campinas, São Paulo, Brazil, in 2017-2018. METHODS: CSF samples collected from adult patients with neurological syndrome symptoms and negative CSF culture results were analyzed using polymerase chain reaction (PCR), reverse transcriptase-PCR, and real-time PCR, and their results were compared with their clinical symptoms. One CSF sample was obtained from each patient. RESULTS: Viral genomes were detected in 148/420 (35.2%) CSF samples: one of 148 (0.2%) was positive for herpes simplex virus-1; two (0.5%) for herpes simplex virus-2; eight (1.9%) for varicella-zoster virus; four (1%) for Epstein-Barr virus; one (0.2%) for cytomegalovirus; 32 (7.6%) for human herpesvirus-6; 30 (7.1%) for non-polio enterovirus; 67 (16.0%) for dengue virus, three (0.7%) for yellow fever virus, and 21 (5%) for Zika virus. CONCLUSIONS: The viral genomes were found in 35.2% of all analyzed samples, showing the high prevalence of viruses in the nervous system and the importance of using a nucleic acid amplification test to detect viral agents in CSF samples.


Subject(s)
Humans , Adult , Arboviruses , Enterovirus/genetics , Epstein-Barr Virus Infections , Zika Virus , Zika Virus Infection , Syndrome , Brazil/epidemiology , DNA, Viral , Herpesvirus 2, Human/genetics , Herpesvirus 4, Human , Herpesvirus 3, Human/genetics , Hospitals, University
5.
Medisan ; 24(1)ene.-feb. 2020. tab
Article in Spanish | LILACS, CUMED | ID: biblio-1091162

ABSTRACT

Introducción: Las enfermedades oftalmológicas originadas por virus ocupan un lugar importante en el quehacer diario de la especialidad. Objetivo: Caracterizar pacientes con infecciones oculares por herpesvirus según variables clínicas y epidemiológicas de interés. Métodos: Se realizó un estudio descriptivo y transversal de 29 pacientes adultos con infecciones por herpesvirus, atendidos en la consulta oftalmológica del Hospital General Docente Dr. Juan Bruno Zayas Alfonso de Santiago de Cuba, desde septiembre de 2012 hasta igual mes de 2013. Resultados: En la casuística predominaron los pacientes de piel blanca (55,1 %), el sexo femenino (69,0 %) y el grupo etario de 57 años y más (48,3 %). El ojo rojo y la inyección cilioconjuntival constituyeron los síntomas y signos más comunes (75,9 %). Las lesiones más frecuentes estuvieron localizadas en los párpados, la piel y la córnea (31,0 %). La afectación de la agudeza visual por debajo de 0,3 estuvo relacionada con la ocurrencia de afectación estromal y la localización de las lesiones en el área pupilar. Ningún paciente presentó inflamación del segmento posterior. Conclusiones: No se encontraron argumentos referidos a la relación existente entre la localización de las lesiones y la afectación de la agudeza visual, lo cual impidió realizar comparaciones.


Introduction: Eye diseases originated due to virus occupy an important place in daily routine of the specialty. Objective: To characterize patients with eye infections due to herpesvirus according to clinical and epidemiological variables of interest. Methods: A descriptive and cross-sectional study of 29 adult patients with infections due to herpesvirus was carried out, they were assisted in the ophthalmologic service of Dr. Juan Bruno Zayas Alfonso Teaching General Hospital in Santiago de Cuba, from September, 2012 to the same month in 2013. Results: In the case material white skin patients (55.1 %), female sex (69.0 %) and 57 years and over age group (48.3 %) prevailed. Red eye and ciliumconjuntival injection constituted the most common symptoms and signs (75.9 %). The most frequent lesions were located in the eyelids, the skin and cornea (31.0 %). The affectation of the visual acuteness below 0.3 was related to the occurrence of stromal affectation and the localization of lesions in the pupilar area. No patient presented inflammation of the later segment. Conclusions: Arguments about the existent relationship between the localization of lesions and the affectation of the visual acuteness were not found, which prevented to carry out comparisons.


Subject(s)
Eye Infections, Viral , Eye Infections, Viral/epidemiology , Herpesvirus 2, Human , Herpesvirus 1, Human , Eye Diseases
6.
Medicina (B.Aires) ; 79(6): 513-515, dic. 2019. tab
Article in Spanish | LILACS | ID: biblio-1056762

ABSTRACT

La meningitis linfocítica recurrente o meningitis de Mollaret es una entidad asociada a un gran número de etiologías infecciosas, autoinmunes, toxicológicas y neoplásicas. En la actualidad el virus herpes simple tipo 2 (HSV-2) es el agente más frecuentemente aislado. Afecta frecuentemente a mujeres de mediana edad y tiende a autolimitarse sin secuelas dentro de la primera semana de inicio de síntomas. El diagnóstico se basa en la detección de ácidos nucleicos virales en el líquido cefalorraquídeo. Al momento no se ha demostrado beneficio en el uso de tratamiento antiviral en la prevención de recurrencias.


Recurrent lymphocytic meningitis or Mollaret´s meningitis is a rare condition caused by a number of infectious, autoimmune, toxic and neoplastic diseases. Herpes simplex type 2 is the most commonly isolated agent. It usually compromises middle aged women, with a self-limited clinical presentation that resolves within a week leaving no sequelae. Its diagnosis is mainly based on nucleic acid detection on cerebrospinal fluid. Antiviral prophylaxis has not shown conclusive to avoid recurrences.


Subject(s)
Humans , Female , Middle Aged , Herpesvirus 2, Human/isolation & purification , Herpes Simplex/complications , Meningitis, Viral/virology , Antiviral Agents , Recurrence , Acyclovir/therapeutic use , Polymerase Chain Reaction , Meningitis, Viral/drug therapy
7.
Rev. bras. neurol ; 55(3): 9-12, jul.-set. 2019. ilus
Article in Portuguese | LILACS | ID: biblio-1022875

ABSTRACT

A meningite recorrente linfocítica benigna ou meningite de Mollaret, inicialmente descrita pelo neurologista francês Pierre Mollaret em 1944, é uma condição relativamente rara, benigna mas incapacitante durante os seus períodos de agudização. Trata-se de quadro inflamatório meníngeo recorrente devido a reativação de infecção pelo herpes simples vírus, particularmente o herpesvirus do tipo 2 (HSV-2). Pode ser reconhecida a partir do seu quadro clínico de meningismo agudo, perfil liquórico linfocítico e identificação do genoma viral por PCR no líquor. Aciclovir e seus derivados podem ser utilizado no seu tratamento ou na sua profilaxia. Sua identificação é importante no sentido de se excluir outras causas de quadros meníngeos recorrentes.


Benign recurrent lymphocytic meningitis or Mollaret's meningitis (MM) was frst described by the French neurologist Pierre Mollaret in 1944. MM is a relatively rare, benign but disabling condition. MM is a recurrent meningeal inflammatory illness due to reactivation of herpes simplex virus infection, particularly herpesvirus type 2 (HSV-2). It can be recognized from its clinical picture of acute meningism, lymphocytic CSF profle and by the identifcation of the viral genome in the CSF by PCR. Acyclovir and its derivatives may be used for its treatment or prophylaxis. The identifcation of MM is important in order to exclude other causes of recurrent meningeal conditions.


Subject(s)
Humans , Female , Adult , Middle Aged , Herpes Simplex/diagnosis , Herpes Simplex/etiology , Meningitis, Aseptic/diagnosis , Meningitis, Aseptic/drug therapy , Acyclovir/therapeutic use , Herpesvirus 2, Human/pathogenicity , Diagnosis, Differential , Neurology/history
8.
Journal of Central South University(Medical Sciences) ; (12): 1143-1150, 2019.
Article in Chinese | WPRIM | ID: wpr-813038

ABSTRACT

To explore the antiviral activity of nano-realgar against herpes simplex virus Type II (HSV-2) in vitro.
 Methods: Acyclovir (ACV) as a positive control, the cytotoxicity of nano-realgar at different concentrations (including 200.00, 150.00, 100.00, 50.00, 25.00, 12.50, 6.25, 3.13, 1.54, 0.78, 0.39 and 0 mg/L) on normal Vero cells were determined by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) assay. HSV-2 virus titer was determined by plaque assay, and the Vero cells model of HSV-2 infection was established. Subsequently, the antiviral effects of nano-realgar at different concentrations (including 20.00, 10.00, 5.00, 2.50, 1.25, 0.63, 0.31, 0.15, 0.08, 0.04 and 0 mg/L) on infected cells model were evaluated by the observation of cytopathic effect (CPE) and MTT method under the 3 modes including pre-treatment, treatment and direct inactivation.
 Results: The 50% cytotoxic concentration (CC50) of nano-realgar on Vero cells was 37.15 mg/L. The titer of HSV-2 was 7.30 log PFUs/mL. In the 3 modes, the half-maximal effective concentration (EC50) of nano-realgar on HSV-2 infected Vero cells were 0.13, 1.80 and 0.52 mg/L, and the corresponding therapeutic index (TI) were 285.77, 20.64, 71.44, respectively. The TI value of nano-realgar on pre-treatment mode was higher than that of nano-realgar on treatment and direct inactivation modes.
 Conclusion: Nano-realgar can play a good anti-HSV-2 activity in the 3 modes (pre-treatment, treatment and direct inactivation), and the anti-HSV-2 efficacy of nano-realgar on pre-treatment mode is better than that of nano-realagr on other 2 modes.


Subject(s)
Animals , Antiviral Agents , Arsenicals , Chlorocebus aethiops , Herpesvirus 1, Human , Herpesvirus 2, Human , Sulfides , Vero Cells
9.
Neonatal Medicine ; : 63-66, 2019.
Article in English | WPRIM | ID: wpr-741662

ABSTRACT

Herpes simplex virus (HSV) is a common pathogen, that causes a broad spectrum of diseases, ranging from minor skin infections to severe encephalitis and widespread infections. Acute retinal necrosis (ARN), one of the most serious manifestations of HSV infection, is defined as a rapidly progressing necrotizing retinopathy that presents discrete areas of circumferential retinal necrosis, along with signs of uveitis, vitreitis, and retinal vasculitis. We encountered a case of a female infant, born at 33 weeks of gestation with a body weight at birth of 2,080 g, who had ARN and encephalomalacia due to HSV infection. ARN associated with HSV infection should be suspected when nonspecific retinal exudates are observed in neonates, especially preterm infants.


Subject(s)
Female , Humans , Infant , Infant, Newborn , Pregnancy , Body Weight , Encephalitis , Encephalomalacia , Exudates and Transudates , Herpes Simplex , Herpesvirus 2, Human , Infant, Premature , Necrosis , Parturition , Retinal Necrosis Syndrome, Acute , Retinal Vasculitis , Retinaldehyde , Simplexvirus , Skin , Uveitis
11.
IBJ-Iranian Biomedical Journal. 2018; 22 (2): 123-128
in English | IMEMR | ID: emr-192459

ABSTRACT

Background: Herpes simplex virus type 2 [HSV-2] is a common infection in human immunodeficiency virus [HIV] patients and may accelerate HIV progression by rising HIV viral load and decreasing CD4 count. However, the available data regarding the influence of HSV-2 seropositivity on HIV progression in HIV individuals are inconclusive. Therefore, we aimed to determine HSV-2 seroprevalence in naive HIV patients and normal controls and also investigate the relation of HIV viral load and CD4 count with HSV-2 seropositivity. Subsequently, we investigated the association of HSV-2 serostatus with changing in CD4 count and HIV viral load in our subjects, after one year follow-up


Methods: In this study, 116 naive HIV patients and 85 healthy controls from Tehran, Iran were enrolled. HSV-2 IgG antibody was detected by ELISA. CD4 count was determined by flowcytometry, and serum HIV RNA copy numbers were determined using real-time PCR


Results: The prevalence of HSV-2 IgG was 18.1% in naive HIV patients and 0% in the control group [P = 0.000]. HSV-2 seroconversion was observed in 2.43% of HIV patients after one year. There was no significant difference regarding HSV-2 serostatus with CD4 count and HIV RNA viral load in our study cohort at baseline and after one year


Conclusion: Our results revealed that the prevalence and incidence of HSV-2 infection are low in our HIV cases, and it is negligible in the control group. However, it seems that HIV/HSV2 co-infection has no role on HIV infection acceleration


Subject(s)
Humans , Male , Female , Adult , HIV Infections , Herpesvirus 2, Human , Herpes Genitalis , Disease Progression , Viral Load , CD4 Lymphocyte Count
12.
Annals of Laboratory Medicine ; : 440-445, 2018.
Article in English | WPRIM | ID: wpr-717056

ABSTRACT

BACKGROUND: Human herpes simplex virus type 1 (HSV-1) and type 2 (HSV-2) are responsible for a plethora of human diseases, of which cutaneous and mucocutaneous infections are the most prevalent. In its most severe form, HSV infection can cause meningitis/encephalitis. We compared the Luminex ARIES HSV 1&2 assay (Luminex Corp., Austin, TX, USA), an automated sample-to-result molecular solution, to two non-automated HSV DNA assays. METHODS: A total of 116 artificial controls were used to determine the analytical performance of the ARIES assay. Controls were prepared by spiking universal transport medium (UTM) and cerebrospinal fluid (CSF) samples from patients who tested negative for HSV by an in-house HSV-1 and -2 DNA assay with reference materials (SeraCare Life Sciences, MA, USA; ZeptoMetrix Corp., MA, USA). Another 117 clinical samples were then used to compare the clinical performance of the ARIES assay with those of an in-house assay and the FTD Neuro 9 assay (Fast Track Diagnostics, Junglinster, Luxembourg). RESULTS: The analytical sensitivity (95% limit of detection) of the ARIES assay was 318 copies/mL (UTM samples) and 935 copies/mL (CSF samples) for HSV-1 strain 96 and 253 copies/mL (UTM samples) and 821 copies/mL (CSF samples) for HSV-2 strain 09. No cross-reactivity was observed in samples spiked with 14 non-HSV microorganisms. Compared with the reference result (agreement between the in-house and FTD Neuro 9 results), the ARIES assay had overall concordance rates of 98.2% (111/113) and 100% (113/113) for HSV-1 and HSV-2, respectively. CONCLUSIONS: The ARIES assay appears to be an excellent alternative for rapid detection and differentiation of HSV in skin and genital infections, meningitis, and encephalitis.


Subject(s)
Humans , Biological Science Disciplines , Cerebrospinal Fluid , DNA , Encephalitis , Herpes Simplex , Herpesvirus 1, Human , Herpesvirus 2, Human , Meningitis , Real-Time Polymerase Chain Reaction , Simplexvirus , Skin
13.
Journal of Cancer Prevention ; : 82-86, 2018.
Article in English | WPRIM | ID: wpr-740100

ABSTRACT

BACKGROUND: Human papillomavirus (HPV) genotypes that infect the genital tract play a main etiologic role in cervical cancer progression. Other environmental factors, such as sexually transmitted diseases and the host genetic pattern, contribute to infection persistence of the uterus and cervical epithelium in sustaining their malignancy. The Janus kinase 2 is a non-receptor tyrosine kinase in cell signaling process of tumor genesis. In the present study, JAK2 V167F mutation was distinguished in women with sexually transmitted infections, such as Herpes simplex virus 2, Chlamydia trachomatis and Mycoplasma genitalium and cervical cancer. METHODS: This case-control survey was performed on 195 liquid based cytology of women specimens. Fifty, 98, and 47 samples were from women with known cervical cancer, HPV positive and HPV negative, respectively. Single nucleotide polymorphism analysis, sexually transmitted infections detection and HPV genotyping were carried out using approved PCR- RFLP, in-house multiplex TaqMan Real Time PCR and the reverse dot blot hybridization assay. RESULTS: HPVs 6, 16, 18, 11, 31, and 51 were the most common genotypes. The prevalence rate of multiple HPV genotypes was 46.0% to 10.1%. Analysis of JAK2 V617F (1849 G > T) showed that prevalence of mutation was GG (65.1%), GA (34.9%), and TT (0%), respectively. There were no statistically significant differences between this mutation and variables of population survey (P ≥ 0.05). CONCLUSIONS: The molecular epidemiology study on the genetic polymorphisms, i.e., JAK2 V617F and other single nucleotide polymorphisms as a diagnostic tool is necessary for cancer screening and prophylactic programs.


Subject(s)
Female , Humans , Case-Control Studies , Chlamydia trachomatis , Early Detection of Cancer , Epithelium , Genotype , Herpesvirus 2, Human , Iran , Janus Kinase 2 , Molecular Epidemiology , Mycoplasma genitalium , Polymorphism, Genetic , Polymorphism, Restriction Fragment Length , Polymorphism, Single Nucleotide , Prevalence , Protein-Tyrosine Kinases , Real-Time Polymerase Chain Reaction , Sexually Transmitted Diseases , Uterine Cervical Neoplasms , Uterus
14.
LMJ-Lebanese Medical Journal. 2018; 66 (1): 57-60
in English | IMEMR | ID: emr-170977

ABSTRACT

Acute transverse myelitis, an acute inflammation of the spinal cord, can be caused by HSV infection. We report a case of an 85-year-old man who presented with lower extremity weakness and flare-up of a vesicular rash on the sacral area. The patient was diagnosed with acute transverse myelitis based on MRI and CSF findings. CSF analysis showed a lymphocytic pleocytosis and positive HSV-2 PCR. The patient was started on intravenous acyclovir, with full clinical and radiologic improvement despite persistence of HSV-2 PCR positivity in the CSF for four months. A review of the literature of HSV myelitis is included. We conclude that testing of HSV PCR on repeated CSF samples might not accurately reflect viral activity in the setting of clinical improvement


Subject(s)
Humans , Male , Aged, 80 and over , Herpesvirus 2, Human , Virus Replication , Polymerase Chain Reaction
15.
Mem. Inst. Oswaldo Cruz ; 113(12): e180328, 2018. tab, graf
Article in English | LILACS | ID: biblio-1040588

ABSTRACT

BACKGROUND Human herpesvirus 2 (HHV-2) have DNA genome with a limited genetic variability and have been classified into two clades. OBJECTIVES To identify and characterise six HHV-2 isolates derived from Brazilian women. METHODS HHV-2 isolates were performed polymerase chain reaction (PCR) and sequencing of 2250 pb of the glycoprotein B (gB) coding regions. FINDINGS Four HHV-2 isolates were classified into clade B, while the remaining two, derived from HIV-1 co-infected women, showed a notable genetic divergence (> 1%). MAIN CONCLUSION The results reveal novel HHV-2 variants. The impact of these novel variants on HHV-2 pathogenesis and HIV/HHV-2 coinfection need to be investigated.


Subject(s)
Humans , Female , Herpes Genitalis/virology , HIV Infections/virology , HIV-1 , Herpesvirus 2, Human/genetics , Genes, Viral/genetics , Phylogeny , Herpes Genitalis/complications , HIV Infections/complications , Polymerase Chain Reaction , Bertholletia , Coinfection/virology
16.
Arq. bras. oftalmol ; 80(2): 84-87, Mar.-Apr. 2017. tab
Article in English | LILACS | ID: biblio-838786

ABSTRACT

ABSTRACT Objective: Bacterial keratitis occurs worldwide, and despite recent developments, it remains a potentially blinding condition. This study assesses the presence of herpes simplex virus (HSV-1 and -2) and varicella zoster virus (VZV) by quantitative real-time polymerase chain reaction (qPCR) in corneal scrapings from patients with bacterial keratitis. Methods: A total of 65 patients with clinical diagnoses of infectious corneal ulcers prospectively underwent clinical eye examinations. Corneal scrapings were investigated by Gram staining, Giemsa staining, culture, and qPCR (the study group). Risk factors and epidemiological data were recorded. The control group comprising 25 eyes with typical herpes dendritic keratitis was also analyzed by qPCR. Results: From the study group (n=65), nine patients (13.8%) had negative smears, cultures, and qPCR findings. Fifty-six (86.2%) patients had positive cultures: 51 for bacteria, 4 for fungi, and 1 for amoebae. Of the patients who had positive bacterial cultures, qPCR identified 10 patients who were also positive for virus: one for VZV and nine for HSV-1. Of the 25 patients in the control group, 21 tested positive for HSV-1 by qPCR analysis. Conclusions: Herpes may be present in patients with bacterial corneal ulcers, and qPCR may be useful in its detection.


RESUMO Objetivo: Ceratites bacterianas ocorrem mundialmente e apesar dos novos desenvolvimentos permanece como uma condição que pode levar à cegueira. Avaliar a presença de herpes simples (-1 e -2) e vírus varicella zoster (VZV) por reação em cadeia quantitativa de polimerase em tempo real (qPCR) em raspados corneanos de pacientes com ceratite bacteriana. Métodos: Sessenta e cinco pacientes com ceratite infecciosa foram submetidos a raspados corneanos estudados para gram, Giemsa, cultura e qPCR (grupo de estudo). Foram avaliados fatores de risco e epidemiológicos. O grupo controle foi composto por 25 casos de úlcera dendrítica típica por herpes analisados por qPCR. Resultados: Do grupo de estudo (n=65), nove pacientes (13,8%) apresentaram cultura, qPCR e raspado negativos. Cinquenta e seis (86,2%) pacientes apresentaram cultura positiva, 51 para bacteria, 4 para fungo e 1 para ameba. A qPCR identificou 10 pacientes do grupo de cultura positiva para bactéria que também foram positivos para vírus, um VZV e 9 para HSV-1. Dos 25 pacientes que compunham o grupo controle, 21 apresentaram qPCR positivo para HSV-1. Conclusão: Herpes pode estar presente em pacientes com úlceras de córnea bacterianas e a qPCR pode ser útil na sua detecção.


Subject(s)
Humans , Male , Female , Child , Adolescent , Adult , Middle Aged , Aged , Aged, 80 and over , Young Adult , Keratitis, Dendritic/microbiology , Herpesvirus 2, Human/isolation & purification , Herpesvirus 1, Human/isolation & purification , Herpesvirus 3, Human/isolation & purification , Cornea/virology , Real-Time Polymerase Chain Reaction/methods , Keratitis/microbiology , DNA Probes , Eye Infections, Bacterial/microbiology , Keratitis, Dendritic/diagnosis , Keratitis, Dendritic/virology , Prospective Studies , Keratitis/diagnosis , Keratitis/virology
17.
Mem. Inst. Oswaldo Cruz ; 112(3): 220-223, Mar. 2017. tab, graf
Article in English | LILACS | ID: biblio-841773

ABSTRACT

The use of quantitative real time polymerase chain reaction (qPCR) for herpesvirus detection has improved the sensitivity and specificity of diagnosis, as it is able to detect shedding episodes in the absence of clinical lesions and diagnose clinical specimens that have low viral loads. With an aim to improve the detection and quantification of herpesvirus by qPCR, synthetic standard curves for human herpesvirus 1 and 2 (HHV-1 and HHV-2) targeting regions gD and gG, respectively, were designed and evaluated. The results show that synthetic curves can replace DNA standard curves in diagnostic herpes qPCR.


Subject(s)
Humans , Herpesvirus 2, Human/genetics , Herpesvirus 1, Human/genetics , Herpes Simplex/virology , DNA, Viral/genetics , Sensitivity and Specificity , Viral Load , Real-Time Polymerase Chain Reaction , Herpes Simplex/diagnosis
18.
An. bras. dermatol ; 91(2): 216-218, Mar.-Apr. 2016. graf
Article in English | LILACS | ID: lil-781357

ABSTRACT

Abstract Neonatal herpes is a serious condition. Newborns can be contaminated in utero via transplacental hematogenic transmission, upon delivery (the most frequent route), or during the postnatal period (indirect transmission). Optimal management requires prompt and accurate recognition, particularly in newborns, in order to prevent complications. Acyclovir is the treatment of choice, but its implementation is often delayed while awaiting test results, such as PCR and serology. Cytology for diagnostic purposes is rarely used in dermatology, despite the quick and reliable results. We report a case of neonatal herpes caused by type 2 herpes simplex virus diagnosed by cytology.


Subject(s)
Humans , Male , Infant, Newborn , Pregnancy Complications, Infectious/pathology , Herpesvirus 2, Human , Herpes Simplex/pathology , Antiviral Agents/therapeutic use , Pregnancy Complications, Infectious/drug therapy , Skin/pathology , Acyclovir/therapeutic use , Cytological Techniques , Herpes Simplex/drug therapy
19.
Journal of the Korean Neurological Association ; : 112-115, 2016.
Article in Korean | WPRIM | ID: wpr-197552

ABSTRACT

BACKGROUND: Herpes simplex virus type 2 (HSV-2) is the second most common cause of viral meningitis and the most common cause of recurrent meningitis. Although the incidence of HSV-2 meningitis is high, its clinical characteristics are not well known. The purpose of this study was to review the clinical characteristics and prognosis of HSV-2 meningitis. METHODS: We analyzed patients who were admitted to the Department of Neurology at Severance Hospital with a final diagnosis of HSV-2 meningitis, as confirmed by applying the polymerase chain reaction to the cerebrospinal fluid (CSF) of patients. RESULTS: The study involved 998 patients with aseptic meningitis and 60 patients diagnosed with HSV-2 meningitis. The mean age at meningitis presentation was 32.5 years (range 18-54 years), and 72% of the patients were female. Common clinical symptoms were headache (100%), nausea and/or vomiting (83%), meningismus (57%), and fever (55%). Six patients had a history of genital herpes infection, and 11 had a past history of recurrent meningitis. The CSF study was notable for elevated protein (111.0±53.5 mg/dL, mean±standard deviation) and white cell count (332.0±211.3 cells/µL). The CSF/serum glucose ratio was 0.52±0.90. Various treatments were applied, including conservative care, antiviral agents, empirical antibiotics, and combined treatments. All patients recovered without serious neurologic sequelae. CONCLUSIONS: HSV-2 meningitis is relatively common, as are recurrent episodes. The clinical characteristics of HSV-2 meningitis are similar to those of other types of aseptic meningitis. HSV-2 meningitis is treated using antiviral therapy, and the prognosis is favorable even with conservative treatment.


Subject(s)
Female , Humans , Anti-Bacterial Agents , Antiviral Agents , Cell Count , Cerebrospinal Fluid , Diagnosis , Fever , Glucose , Headache , Herpes Genitalis , Herpes Simplex , Herpesvirus 2, Human , Incidence , Meningism , Meningitis , Meningitis, Aseptic , Meningitis, Viral , Nausea , Neurology , Polymerase Chain Reaction , Prognosis , Retrospective Studies , Simplexvirus , Vomiting
20.
Journal of the Korean Neurological Association ; : 346-349, 2016.
Article in Korean | WPRIM | ID: wpr-179066

ABSTRACT

We describe two patients with acute myeloradiculitis associated with herpes simplex virus type 2 (HSV-2). They were previously healthy and immunocompetent and had no history of herpes infection or rash. Myeloradiculitis manifested as an acute flaccid paralysis that primarily involved the conus medullaris and cauda equina. laccid paralysis can be caused by HSV-2 myeloradiculitis, and so early antiviral treatment should be considered.


Subject(s)
Humans , Cauda Equina , Exanthema , Herpes Simplex , Herpesvirus 2, Human , Myelitis , Paralysis , Radiculopathy , Simplexvirus , Spinal Cord
SELECTION OF CITATIONS
SEARCH DETAIL