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1.
Lima; Perú. Ministerio de Salud; 20210500. 39 p. tab.
Monography in Spanish | LILACS, MINSAPERU | ID: biblio-1121794

ABSTRACT

La publicación describe los parámetros técnicos estandarizados, para la evaluación de la afectación psicológica de personas, en caso de haber sido víctimas de violencia contra las mujeres y los integrantes del grupo familiar y para contribuir a la recuperación integral y oportuna de las personas víctimas de violencia contra las mujeres e integrantes del grupo familiar que acuden a las Instituciones Prestadoras de Servicios de Salud (IPRESS).


Subject(s)
Reference Standards , Guidelines as Topic , Violence Against Women , Reference Parameters , Jurisprudence
2.
Nursing (Säo Paulo) ; 24(275): 5544-5555, abr.-2021.
Article in Portuguese | LILACS, BDENF | ID: biblio-1224355

ABSTRACT

Objetivo: Descrever as ações do enfermeiro no atendimento ao portador de feridas na atenção básica em saúde. Método: Revisão integrativa estruturada em seis etapas a partir da busca de artigos de livre acesso nas bases de dados publicados no intervalo de 10 anos (2009-2019), baseado em critérios de elegibilidade estabelecidos e no nível de evidência das publicações. Resultados: Os estudos incluídos para análise apontam a atuação do enfermeiro centrada na garantia de espaço físico em conformidade com as normas sanitárias, na educação em saúde como ação de promoção da autonomia e autocuidado e na observação dos aspectos sociais e culturais do usuário no contexto da melhoria da cicatrização das lesões. Conclusões: Entende-se como necessária a inserção deste profissional enquanto participante ativo da assistência para desenvolvimento de ações respaldadas e na possibilidade da redução do tempo de tratamento do usuário e dos custos da assistência junto ao sistema de saúde.(AU)


Objective: To describe the actions of nurses in the care of patients with wounds in primary health care. Method: Integrative review structured in six stages based on the search for freely accessible articles in the databases published over a 10-year period (2009-2019), based on established eligibility criteria and the level of evidence of the publications. Results: The studies included for analysis point out the role of nurses focused on guaranteeing physical space in accordance with health standards, in health education as an action to promote autonomy and self-care and in observing the social and cultural aspects of the user in the context of improvement of wound healing. Conclusions: It is understood as necessary to insert this professional as an active participant in the assistance for the development of supported actions and in the possibility of reducing the user's treatment time and the costs of assistance with the health system.(AU)


Objetivo: Describir las acciones del enfermero en la atención de pacientes con heridas en la atención primaria de salud. Método: Revisión integradora estructurada en seis etapas a partir de la búsqueda de artículos de libre acceso en las bases de datos publicadas durante un período de 10 años (2009-2019), en base a los criterios de elegibilidad establecidos y al nivel de evidencia de las publicaciones. Resultados: Los estudios incluidos para análisis señalan el rol del enfermero enfocado en garantizar el espacio físico de acuerdo con los estándares de salud, en la educación para la salud como una acción para promover la autonomía y el autocuidado y en la observación de los aspectos sociales y culturales del usuario en el contexto de mejora de la cicatrización de heridas. Conclusiones: Se entiende necesario insertar a este profesional como participante activo en la asistencia para el desarrollo de acciones apoyadas y en la posibilidad de reducir el tiempo de tratamiento del usuario y los costos de asistencia con el sistema de salud.(AU)


Subject(s)
Humans , Primary Health Care , Wounds and Injuries , Health Education/standards , Nursing Care , Reference Standards , Self Care , Wound Healing
3.
Arch. argent. pediatr ; 119(2): 91-98, abril 2021. tab, ilus
Article in English, Spanish | LILACS, BINACIS | ID: biblio-1151240

ABSTRACT

Objetivo. Evaluar la reproductibilidad de las pruebas de competencia motriz (CM) de transposición lateral y salto lateral, y estimar percentiles por edad y sexo para niños que viven a altitud modera del Perú.Método. Se efectuó un estudio descriptivo transversal en la provincia de Arequipa (Perú). Se evaluó el peso, la estatura y la circunferencia de cintura. Se calculó el índice de masa corporal e índice ponderal. Se evaluó la prueba de transposición lateral y salto lateral (ambas de la batería Kopfer test für Kinder).Resultados. La muestra estuvo constituida por 885 niñas y 897 niños de 6,0 a 16,9 años. El error técnico de medida intraevaluador para ambas pruebas de CM oscilaron entre 1,75 y 3,9 repeticiones en ambos sexos, mientras que el coeficiente de correlación intraclase fue de 0,77 a 0,99. Los límites de acuerdo oscilaron entre -7,3 y 6,8 repeticiones en ambas pruebas. Se estimaron percentiles 5º, 15º, 50º, 85º y 95º por el método Least-Mean-Square algorithm. Los puntos de corte adoptados para las pruebas CM fueron bajo: < p15; regular: de p15 a p85; y alto: > p85.Conclusión. Las pruebas de transposición lateral y salto lateral mostraron una elevada capacidad de reproductibilidad. Los percentiles propuestos pueden servir para valorar la CM y podrían ser incluidos y adaptados como indicadores de desempeño en la educación física.


Objective. To assess the reproducibility of two motor competence (MC) tests: moving sideways and jumping sideways, and to estimate age and sex percentiles for children living at moderate altitude in Peru.Method. This was a descriptive, cross-sectional study conducted in the province of Arequipa, Peru. Weight, height, and waist circumference were assessed. Body mass index and ponderal index were estimated. The moving sideways and jumping sideways tests were assessed (both from the Körper test für Kinder test battery).Results. The sample was made up of 885 girls and 897 boys aged 6.0-16.9 years. The intra-evaluator technical error of measurement for both MC tests ranged between 1.75 and 3.9 repetitions in both males and females, whereas the intraclass correlation coefficient was 0.77-0.99. Agreement limits ranged between -7.3 and 6.8 repetitions for both tests. The 5th, 15th, 50th, 85th, and 95th percentiles were estimated using the Least-Mean-Square algorithm. The cut-off points for MC tests were low: < p15; medium: p15-p85; and high: > p85. Conclusion. Moving sideways and jumping sideways showed a high capacity for reproducibility. The proposed percentiles may be useful to assess MC and could be included and adapted as performance indicators in physical education


Subject(s)
Humans , Male , Female , Child , Adolescent , Reproducibility of Results , Motor Skills , Peru , Physical Education and Training , Reference Standards , Epidemiology, Descriptive , Cross-Sectional Studies , Altitude
5.
Rev. méd. Urug ; 37(1): e502, mar. 2021.
Article in Spanish | LILACS, BNUY | ID: biblio-1180964

ABSTRACT

Resumen: La transfusión de sangre ha sido identificada como uno de los procedimientos médicos más sobreutilizados en pacientes hospitalizados. Una estrategia que permitiría reducir la exposición de los pacientes a la sangre es a través de la implementación de una política de transfusión de una sola unidad, en lugar de dos, para pacientes estables, sin hemorragia y con anemia sintomática. La decisión de transfundir sangre es compleja y depende de varios factores, además, los riesgos de la transfusión se deben sopesar contra los beneficios esperados. La transfusión de dos unidades de sangre a la vez ya no se considera una forma de tratamiento "estándar" para pacientes anémicos sin sangrado activo. Existe una gran oportunidad para reducir el uso excesivo de la transfusión y fomentar las transfusiones de una sola unidad.


Summary: Blood transfusion has been identified as one of the most overused medical procedures in hospitalized patients. One strategy that would reduce the exposure of patients to blood is through the implementation of a single-unit regime for stable patients without bleeding and with symptomatic anemia. The decision to transfuse blood is complex and depends on several factors, in addition, the risks of transfusion must be weighed against the expected benefits. Transfusion of two units of blood at the same time is no longer considered a "standard" form of treatment for anemic patients without active bleeding. There is a great opportunity to reduce the overuse of transfusion and encourage single-unit transfusions.


Resumo: A transfusão de sangue foi identificada como um dos procedimentos médicos usados de mais em pacientes hospitalizados. Uma estratégia que reduziria a exposição dos pacientes ao sangue é por meio da implantação de uma política transfusional de unidade única para pacientes estáveis, sem sangramento e com anemia sintomática. A decisão de transfundir sangue é complexa e depende de vários fatores, além disso, os riscos da transfusão devem ser analisados em relação aos benefícios esperados. A transfusão de duas unidades de sangue ao mesmo tempo não é mais considerada uma forma "padrão" de tratamento para pacientes anêmicos sem sangramento ativo. Há uma grande oportunidade de reduzir o uso excessivo de transfusões e incentivar as transfusões de uma única unidade.


Subject(s)
Reference Standards , Blood Transfusion
6.
Acta odontol. Colomb. (En linea) ; 11(1): 42-58, 2021. tab, ilus, ilus, ilus, ilus, ilus
Article in Spanish | LILACS, COLNAL | ID: biblio-1128590

ABSTRACT

Objetivo: desarrollar y aplicar un manual para evaluar los procesos de deglución y rendimiento masticatorio, dirigido a estudiantes y profesionales de odontología. Método: se realizó un estudio con enfoque cuali-cuantitativo, a partir de dos técnicas de recolección de información: documental para reunir información de los procedimientos y observacional para la aplicación del manual. Los métodos seleccionados fueron el rendimiento masticatorio (Albert T) y de deglución (Técnica Payne); el diseño se esbozó según la metodología para mejorar la calidad de los procesos y una guía técnica de elaboración de manuales de procedimientos en salud. Así, el manual cuenta con introducción, antecedentes históricos, alcance y objetivo, flujograma, descripción de procedimientos de evaluación de la deglución, del rendimiento masticatorio y bibliografía. Este fue aplicado en 27 pacientes de la clínica de ortodoncia, a quienes se les diagnosticó deglución atípica, y se midió el rendimiento masticatorio para conocer la mediana de tamaño de partícula (MTP) de cada individuo. Resultados: el manual se realizó basándose en dos procedimientos, uno con el diagnóstico de deglución y otro con rendimiento masticatorio. Este último fue aplicado por dos estudiantes investigadores a una muestra de 27 pacientes, cuyo resultado fue una mediana de tamaño total de partícula de MTP = 5.35 mm2. Hubo una diferencia estadísticamente significativa entre sexos (MTP en hombres: 6,0 mm2 y mujeres 5.1 mm2) siendo las mujeres quienes presentaron mejor desempeño masticatorio. Conclusión: al aplicar el manual, los estudiantes evaluaron el rendimiento masticatorio y la deglución, con lo cual lograron resultados medibles, aplicables y reproducibles.


Objective: To apply a manual to evaluate swallowing and chewing performance aimed at dental students and professionals. Method: A study was carried out with a qualitative-quantitative approach developing two information gathering techniques: documentary to gather information on procedures and observational in the application of the manual. The methods of chewing performance (Albert T) and swallowing (Payne Technique) were selected; the design was outlined according to the methodology to improve the quality of the processes and a technical guide for the elaboration of manuals of health procedures; in the development phase, the thematic units were created and the manual was prepared with: cover, back cover, authors, introduction, historical background, scope and objective, flow chart, description of swallowing evaluation procedures, chewing performance and bibliography; this was applied to 27 patients from the orthodontic clinic, who were diagnosed with atypical swallowing, and the masticatory performance was measured to determine the median particle size (MTP) of each individual evaluated. Results: The manual was made based on two procedures, one with swallowing diagnosis and the other with masticatory performance, which was applied by two student researchers to a sample of 27 patients, yielding a median total particle size of MTP = 5.35 mm2. There was a statistically significant difference between the sexes (MTP in men: 6.0 mm2 and women 5.1 mm2), with women presenting the best masticatory performance Conclusion: when applying the manual, the students evaluated the chewing performance and swallowing, achieving measurable, applicable and reproducible.


Subject(s)
Humans , Deglutition/physiology , Mastication/physiology , Reference Standards , Students, Dental , Methodology , Malocclusion , Mastication
7.
Clinics ; 76: e2740, 2021. tab, graf
Article in English | LILACS | ID: biblio-1153947

ABSTRACT

OBJECTIVES: The intrathecal route has not yet been thoroughly standardized and evaluated in an experimental model of spinal cord injury (SCI) in Wistar rats. The objective of this study was to standardize and evaluate the effect of intradural injection in this animal model. METHOD: The animals were divided into 6 groups: 1) laminectomy and intradural catheter; 2) laminectomy, intradural catheter and infusion; 3) only SCI; 4) SCI and intradural catheter; 5) SCI, intradural catheter and infusion; and 6) control (laminectomy only). Motor evaluations were performed using the Basso, Beattie and Bresnahan (BBB) scale and the horizontal ladder test; motor evoked potentials were measured for functional evaluation, and histological evaluation was performed as well. All experimental data underwent statistical analysis. RESULTS: Regarding motor evoked potentials, the groups with experimental SCI had worse results than those without, but neither dural puncture nor the injection of intrathecal solution aggravated the effects of isolated SCI. Regarding histology, adverse tissue effects were observed in animals with SCI. On average, the BBB scores had the same statistical behaviour as the horizontal ladder results, and at every evaluated timepoint, the groups without SCI presented scored significantly better than those with SCI (p<0.05). The difference in performance on motor tests between rats with and without experimental SCI persisted from the first to the last test. CONCLUSIONS: The present work standardizes the model of intradural injection in experimental SCI in rats. Intrathecal puncture and injection did not independently cause significant functional or histological changes.


Subject(s)
Animals , Rats , Spinal Cord Injuries , Reference Standards , Spinal Cord , Rats, Wistar , Evoked Potentials, Motor , Recovery of Function , Disease Models, Animal
8.
Rev. Fac. Odontol. (B.Aires) ; 36(83): 27-33, 2021. ilus, graf
Article in Spanish | LILACS | ID: biblio-1342881

ABSTRACT

El desarrollo de los dientes representa el criterio más utilizado para estimar la edad en niños y adultos jóvenes. El método de Cameriere et al., aceptado y validado internacionalmente, evalúa radiográficamente la edad dental a través de mediciones de dientes con el ápice abierto y la aplicación de ecuaciones de regresión específicas. En el presente estudio se analizaron 7 dientes mandibulares (excepto los terceros molares) en 122 radiografías panorámicas digitales mediante el programa ImageJ, de una muestra de ciudadanos argentinos de 5 a 19 años (63 masculinos y 54 femeninas) con el objetivo de evaluar la precisión del método propuesto por Cameriere et al. Se registraron los datos de sexo y edad de las ortopantomografías en forma ciega de la base de datos. Se desarrollaron modelos de regresión lineal para la estimación de la edad. Los resultados demostraron que el sistema es confiable para la estimación de la edad en la población estudiada, con un error absoluto medio de 0.014 años entre edades cronológicas y estimadas (p=0,603). Hubo una ligera tendencia a sobreestimar las edades de 5 a 10 años y a subestimar las edades de 11 a 16 años. Se considera apropiado efectuar nuevas investigaciones para obtener mayores conclusiones y contribuir a la estandarización del método Cameriere con fines forenses en Argentina (AU)


Subject(s)
Humans , Male , Female , Child, Preschool , Child , Adolescent , Adult , Age Determination by Teeth , Forensic Dentistry , Argentina/epidemiology , Reference Standards , Radiography, Panoramic , Logistic Models , Dental Service, Hospital , Age and Sex Distribution
9.
Rev. CEFAC ; 23(2): e11120, 2021. tab, graf
Article in English | LILACS | ID: biblio-1155328

ABSTRACT

ABSTRACT Purpose: to verify, with the auditory-perceptual and acoustic parameters, the occurrence of voice changes in children with no voice complaints, per sex. Methods: the registers of 36 children with no voice complaints, aged 6 to 8 years, of whom 19 were males, were assessed. The databank consisted of the recordings of sustained vowels, sentences, and spontaneous speech. The auditory-perceptual analysis was conducted with the Consensus Auditory-Perceptual Evaluation of Voice, and the acoustic analysis, with VoxMetria. The fundamental frequency, jitter, shimmer, noise, and glottal-to-noise excitation ratio were analyzed. The Mann-Whitney test was applied for numerical variables, and the McNemar test, for the categorical ones, at the p < 0.05 significance level. Results: of the 5 children with mild voice changes, 4 were males. The most frequent resonance deviation was the laryngopharyngeal one. There was no difference between the sexes in the auditory-perceptual findings. The fundamental frequency in the group with changes was lower than that in the group without changes. There was a statistical association between the changes in the auditory-perceptual assessment and the acoustic parameters. Conclusion: voice changes occurred in 13.89% of the studied sample of children with no voice complaints, without differences between the sexes in the auditory-perceptual parameters. Vocal deviations were in a mild degree in all children who had them, the most frequent ones being roughness and breathiness. The fundamental frequency and shimmer were the acoustic parameters whose values most often occurred outside the reference limits, in both genders.


RESUMO Objetivo: verificar a ocorrência de alterações vocais por seus respectivos parâmetros perceptivo-auditivos e acústicos em crianças sem queixas de voz, segundo o sexo. Métodos: foram avaliados os registros de 36 crianças sem queixas de voz, com idade entre seis e oito anos, sendo 19 do sexo masculino. O banco de dados consistiu de gravações de vogais sustentadas, frases e fala espontânea. A análise perceptivo-auditiva foi realizada por meio do Consensus Auditory-Perceptual Evaluation of Voice e a análise acústica por meio do VoxMetria. Foram analisados: frequência fundamental, jitter, shimmer, ruído e glottal to noise excitation ratio e aplicados os testes Mann-Whitney, para as variáveis numéricas, e McNemar, para as categóricas, com nível de significância de p<0,05. Resultados: das cinco crianças com alterações vocais leves, quatro eram do sexo masculino. O desvio de ressonância mais ocorrente foi o laringofaríngeo. Não houve diferença entre os sexos, nos achados perceptivo-auditivos. A frequência fundamental do grupo com alterações foi menor em relação ao grupo sem alterações. Houve associação estatística entre as alterações na avaliação perceptivo-auditiva e nos parâmetros acústicos. Conclusão: a ocorrência de alterações vocais na população de crianças estudadas sem queixa vocal foi de 13,89% e não diferiu entre os sexos nos parâmetros perceptivo-auditivos. Das crianças que apresentaram desvios vocais, todos foram em grau leve, com rugosidade e soprosidade mais ocorrentes. A frequência fundamental e o shimmer foram os parâmetros acústicos com maior ocorrência de valores fora dos limites referenciais, em ambos os sexos.


Subject(s)
Humans , Male , Female , Child , Auditory Perception/physiology , Voice Disorders/diagnosis , Reference Standards , Sex Factors , Cross-Sectional Studies , Retrospective Studies
10.
Article in Chinese | WPRIM | ID: wpr-880474

ABSTRACT

OBJECTIVE@#Thinking on the construction of the medical device type archives information system.@*METHODS@#This paper introduces the concept and significance of medical device variety archives, and puts forward the overall construction idea and system framework of medical device variety archives by analyzing its construction difficulties.@*RESULTS@#Considering the long-term nature and complexity of the construction of medical device variety archives, the system can be constructed in accordance with the three steps of system building, platform building and data management, and the overall technical architecture can be designed from the eight aspects of user layer, business application layer, application support layer, data resource layer, infrastructure layer, security, standards and operation and maintenance management.@*CONCLUSIONS@#Architecture design is the foundation of system construction, and its design rationality is very important for the success of system construction. The architecture design proposed in this study has a certain reference role for promoting the construction of medical device variety archives management system.


Subject(s)
Information Systems , Reference Standards
11.
Article in Chinese | WPRIM | ID: wpr-880452

ABSTRACT

The registration system of medical device Master Files is established to solve the problem that the outsourcing suppliers are not willing to cooperate with the device applicants in the process of providing medical device application documents. After a brief introduction of Master Files systems established by foreign regulatory agencies, this article focuses on the research of establishing a medical device Master Files registration system in China. The results show that the establishment of Chinese Master Files registration system can both improve the standardization and convenience of outsourcing activities of medical devices, and satisfy the needs of the development of medical device industry and regulatory system. At the same time, the probability of additional risk caused by the implementation of the system is low. Therefore, it is expected that the benefits of the system to promote public health outweigh the potential risks, which demonstrates that establishment of the system has important application values.


Subject(s)
China , Industry , Reference Standards
12.
Article in Chinese | WPRIM | ID: wpr-880450

ABSTRACT

OBJECTIVE@#To understand the current situation of artificial intelligence production enterprise quality management system, so as to provide reference basis for the research and standardization of Artificial Intelligence Medical Device (AIMD) product quality management.@*METHODS@#Based on YY/T 0287-2017 Medical Device Quality Management System for Regulatory Requirements, Medical Equipment Production and the Quality Control Standard for Independent Software Appendix and Xavier GMLP report, the relevant factors were screened and the questionnaire was designed by combining expert consultation and literature review. Then, a total of 32 representative AIMD enterprises were invited to fill in the questionnaire. Descriptive statistical analysis was performed on the data results using Excel 2016.@*RESULTS@#Through in-depth analysis of the four themes in product planning and design, result output, product quality control and product change, it was found that it was necessary for enterprises participating in the survey to improve the quality management system of AIMD products to different degrees.@*CONCLUSIONS@#This study is the first time to systematically investigate the status quo of quality management of AIMD enterprises. The result will be useful for the establishment and continuous improvement of product quality management system. It will also provide a reference for the research of AIMD product quality management and the establishment of the standard.


Subject(s)
Artificial Intelligence , Quality Control , Reference Standards , Software
13.
Article in Chinese | WPRIM | ID: wpr-880429

ABSTRACT

Summarize the quality status and variety quality change characteristics of the sampling products through the Summary and analysis, according to the results of the national medical device supervision and inspection in 2019. Put forward suggestions on the development of the medical device industry and supervisory measures. Thereby, further improve the level of the medical device and ensure the safety use of medical device.


Subject(s)
Equipment and Supplies , Industry , Reference Standards
14.
Article in Chinese | WPRIM | ID: wpr-879064

ABSTRACT

To analyze quality standards of cattle bile and sheep bile, and to discuss the related problems in the standards. The results showed that physical forms of the related medicinal materials of cattle bile and sheep bile were chaotic, and the technical methods adopted in the quality standards were generally backward. In addition, there were still problems that some medicinal material standards lacked necessary test items, which were especially obvious in the relevant medicinal material standards of sheep bile and brought difficulties to quality evaluation and control. We suggest that physical forms of cattle bile and sheep bile in quality standards should be determined, and inspection items should be completed. Based on mainstream analytical technology, some technical methods of these standards should be improved.


Subject(s)
Animals , Bile , Cattle , Reference Standards , Sheep , Steroids
15.
Article in Chinese | WPRIM | ID: wpr-879046

ABSTRACT

The extract rates, multicomponent content and fingerprint were determined in this study to investigate the quality diffe-rence between standard decoction of raw Paeoniae Radix Alba and fried Paeoniae Radix Alba. UPLC fingerprint was established for 17 batches of standard decoction of raw and fried Paeoniae Radix Alba, and the contents of gallic acid, catechin, albiflorin, paeoniflorin and benzoyl paeoniflorin were determined. The peak areas of standard decoction were analyzed by the independent t-test and orthogonal partial least squares discriminant analysis. There was no significant difference in extract rates between the standard decoction of raw and fried Paeoniae Radix Alba. After fried processing, the content of albiflorin increased by 0.26%, while the contents of gallic acid, catechin, paeoniflorin and benzoyl paeoniflorin decreased by 13.04%, 27.97%, 10.30% and 18.79% respectively. There were 14 common peaks in the fingerprint of standard decoction of raw Paeoniae Radix Alba, and 16 common peaks in the fried Paeoniae Radix Alba. Peak 1 and peak 3 were new ones after processing, among which the peak 3 was 5-hydroxymethylfurfural. The results showed that peak 1, peak 3, peak 11 and peak 15 were the key compounds to distinguish standard decoction of raw and fried Paeoniae Radix Alba. In conclusion, this method is stable and can be used for the study of quantity transfer and quality control in the preparation process of standard decoction, granules and other dosage forms for raw and fried Paeoniae Radix Alba, providing reference for the identification of raw and fried Paeoniae Radix Alba and related preparations.


Subject(s)
Chromatography, High Pressure Liquid , Drugs, Chinese Herbal , Paeonia , Quality Control , Reference Standards
16.
Article in Chinese | WPRIM | ID: wpr-878997

ABSTRACT

On the basis of literatures and standards relating to Tibetan medicine, the varieties, origin, standards and efficacy of Saxifragaceae plant used in Tibetan medicine were summarized. According to the findings, 75 species(including varieties) in 8 genera of Saxifragaceae plants, involving 21 varieties, are used in Tibetan medicine. Among them, 9 commonly used varieties, namely Songdi, Sedi, Yajima, Aoledansaierbao, Jiansidawu, Saiguo, Katuer, Sangdi, Maoqinghong, are recorded in Chinese Pharmacopoeia, Ministry Standards for Tibetan Medicine, Tibetan Medicine Standards and other local standards, accounting for 42.9% of the total number of varieties. Tibetan names, Tibetan translation of Chinese names, as well as original plant of Tibetan medicine varieties are quite different in relevant Tibetan medicine standards and literatures, which resulted in common phenomena of synonym and homonym. The standards of most varieties only involve characters, and microscopic, physical and chemical identification, with low quality standards. Based on the results of the analysis, this paper suggests strengthening surveys on herbal textual research, resources and current utilization of Saxifragaceae plants used in Tibetan medicine, summarizing the varieties, establishing improved quality standards, and perfor-ming a comparative study on therapeutic material basis and biological activity of different original plants, in order to promote rational use of these medicinal plant resources, and ensuring the accuracy, safety, and effectiveness of clinical medication.


Subject(s)
Asian Continental Ancestry Group , Drugs, Chinese Herbal , Humans , Medicine, Tibetan Traditional , Plants, Medicinal , Reference Standards , Saxifragaceae
17.
Article in Chinese | WPRIM | ID: wpr-878959

ABSTRACT

Amana edulis is a traditional Chinese medicinal plant with low propagation coefficient. In recent years, the increasing demands of A. edulis lead to a shortage of its wild resources. In order to analyze the expression of related functional genes in A. edulis, the selection of suitable internal reference genes is crucial to improve the accuracy of experimental results. Eight genes(ACT, TUA, CYP, GAPDH, UBQ, UBI, EF1a, UBC)were chosen as candidate reference genes based on the RNA-Seq. Real-time fluorescence quantitative technique was used to detect the expression level of candidate internal reference genes in different organs(bulb, leaf, flo-wer) and stolons at different development stages of A. edulis. Then GeNorm, NormFinder, BestKeeper softwares and RefFinder website were used for a comprehensive analysis of the expression stability of the candidate genes.The results showed that among the 8 candidate reference genes, the variation range of Ct value of UBC was the smallest, and the expression level was stable, which was suitable for an reference gene. GeNorm and NormFinder software analysis showed that UBC and UBI were the optimal reference genes. BestKeeper analysis showed that CYP and UBC expression were relatively stable. Comprehensive evaluation of RefFinder website showed that UBC and UBI were the most stable genes, and ACT displayed the lowest stability in all software evaluation, indicating UBC and UBI were suitable for reference genes. Additionally, the most stable UBC, UBI and the most unstable ACT were used as internal reference genes to detect the expression of GBSS gene in A. edulis, and expression pattern of GBSS gene was the same under the calibration of UBC and UBI. The expression data of GBSS gene confirmed that UBC and UBI genes were reliable for A. edulis qRT-PCR as internal reference genes. The results would benefit future studies on related gene expression of A. edulis.


Subject(s)
Gene Expression Profiling , Gene Expression Regulation, Plant , Genes, Plant/genetics , Real-Time Polymerase Chain Reaction , Reference Standards
18.
Article in Chinese | WPRIM | ID: wpr-878946

ABSTRACT

To verify the appropriate preparation process of extracts for the solid substance benchmark of Linggui Zhugan Decoction. The extracts were prepared by different preparation processes, namely the traditional process(process 1), the extract combined with volatile oil separated from traditional process extract liquid(process 2), the modern secondary reflux extraction process(process 3) and the process that volatile oil was extracted first, then prepared according to the traditional process, and combined with extract(process 4); based on the characteristic spectrum, index components of cinnamaldehyde, glycyrrhizin, ammonium glycyrrhizinate, cinnamic acid, and the dry extract rate of process 1, the differences and similarities of four extracts were compared. The results showed that the similarity of the characteristic spectrum of process 2, process 4 and process 1 were all greater than 0.97, while there was no significant difference for the content of 4 quality control components and dry extract rate; the similarity of the characteristic spectrum of process 3 and process 1 was 0.91, the absolute peak area of 13 out of 21 peaks and the relative peak area of 7 peaks increased significantly, and the content of 3 out of 4 quality control components and dry extract rate also significantly increased. In conclusion, the material standards of extracts by the process 2 and 4 are consistent with that of the traditional process, so the two processes are suitable.


Subject(s)
Chromatography, High Pressure Liquid , Drugs, Chinese Herbal , Glycyrrhizic Acid , Oils, Volatile , Quality Control , Reference Standards
19.
Article in Chinese | WPRIM | ID: wpr-878914

ABSTRACT

To select suitable references gene of Polygonum multiflorum for gene expression analysis in different tissues, five candidate reference genes like Actin,GAPDH,SAND,PP2A,TIP41 were selected from the transcriptome data of P. multiflorum, then the specific primers were designed. The expression stability of the five reference genes in different tissues of P. multiflorum was analyzed by Real-time quantitative PCR through avilable analysis methods such as geNorm, NormFinder, BestKeeper, Delta CT and RefFinder, to ensure the reliability of the analysis results. The results showed that there were significant differences in the expression levels and stability of candidate genes in different tissues of P. multiflorum. Ct distribution analysis of the expression levels of candidate genes showed that the expression levels of Actin and GAPDH genes were relatively high in different tissues, while the expression levels of SAND, PP2A and TIP41 were lower. The stability of each candidate gene was analyzed by different methods. The results of geNorm analysis showed that the expression of PP2A and GAPDH was the most stable, the expression stability of SAND was the worst, the stability of PP2A was the highest in both NormFinder and Delta CT, the stability of SAND was the lowest, and the stability of Actin was the most stable in BestKeeper analysis. Through the comprehensive evaluation and analysis of the stability of candidate genes by RefFinder, it is concluded that the stability of PP2A gene is the highest, followed by GAPDH, Actin, TIP41, SAND, and SAND gene is the worst. Therefore, the PP2A gene is an ideal reference gene for the analysis of gene expression in different tissues of P. multiflorum.


Subject(s)
Fallopia multiflora , Gene Expression Profiling , Gene Expression Regulation, Plant , Genes, Plant/genetics , Real-Time Polymerase Chain Reaction , Reference Standards , Reproducibility of Results
20.
Article in Chinese | WPRIM | ID: wpr-878902

ABSTRACT

Standards for seeds and seedlings of Chinese materia medica are very important to the production, quality control and management of seeds and seedlings of Chinese materia medica. In this paper, the current standards including international standards, national standards, industry standards, local standards and group standards before 2020, involving relevant standards for seeds and seedlings of Chinese materia medica, were comprehensively and systematically summarized. Relevant standards for seeds and seedlings of Chinese materia medica were analyzed based on the standard issue year, the source and types of Chinese materia medica, and whether they are included in the Pharmacopoeia of the People's Republic of China 2015. It is suggested that the standards for seed and seedling of Chinese materia medica shall be systematic, professional and feasible, so as to ensure the sould and sustainable development of the seed and seedling industry of Chinese materia medica.


Subject(s)
China , Drugs, Chinese Herbal , Humans , Materia Medica , Medicine, Chinese Traditional , Reference Standards , Seedlings , Seeds
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