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Infectio ; 25(3): 145-152, jul.-set. 2021. tab, graf
Article in English | LILACS, COLNAL | ID: biblio-1250084


Abstract Objectives: To determine the prevalence of antibodies to SARS-CoV-2 and the incidence of seroconversion in the first month of follow-up among interns, residents, and medical doctors attending patients at a University Hospital in Bogota (Colombia). Design or methods: A cross-sectional and a prospective study were performed during June, July, and August 2020 to assess seroprevalence and seroconversion rates using CLIA IgG for SARS-CoV-2. LFA IgG and IgM and ELFA IgM were also determined to explore concordance with CLIA IgG. Results: At baseline, 8 (2.28% 95%CI 1.16-4.43%) participants were IgG positive for SARS-CoV-2 by CLIA. At the end of the study, 21 (5.98% 95%CI 3.94-8.97%) individuals seroconverted by CLIA IgG. In all, 29 individuals had IgG by CLIA and of these 11 (3.13% 95%CI 1.76-5.52%) were asymptomatic. No associations with risk factors for infection were identified. CLIA IgG had moderate concordance (>962 samples) with LFA IgG and ELFA IgM, but minimal with LFA IgM. Conclusions: Our report is the first in Latina America on seroprevalence and seroconversion rates in medical healthcare workers. The relatively high rate (>3%) of asymptomatic health care workers with evidence of previous SARS-CoV-2 infection underscores the need to screen this population for infection to prevent infection/disease spread.

Resumen Objetivos: Determinar la prevalencia de anticuerpos frente al SARS-CoV-2 y la incidencia de seroconversión en el primer mes de seguimiento en internos, residentes y médicos que atienden pacientes en un Hospital Universitario de Bogotá (Colombia). Diseño y métodos: Se realizó un estudio transversal y prospectivo durante junio, julio y agosto de 2020 para evaluar las tasas de seroprevalencia y seroconversión utilizando CLIA IgG para SARS-CoV-2. También se determinaron LFA IgG e IgM y ELFA IgM para explorar la concordancia con CLIA IgG. Resultados: Al inicio del estudio, 8 (2,28% IC del 95% 1,16-4,43%) participantes fueron IgG positivos para SARS-CoV-2 por CLIA. Al final del estudio, 21 (5,98% IC 95% 3,94-8,97%) individuos seroconvirtieron por CLIA IgG. En total, 29 individuos tenían IgG por CLIA y de estos 11 (3,13% 95% IC 1,76-5,52%) eran asintomáticos. No se identificaron asociaciones con factores de riesgo de infección. El CLIA IgG tuvo una concordancia moderada (> 962 muestras) con LFA IgG y ELFA IgM, pero mínima con el LFA IgM. Conclusiones: Nuestro informe es el primero en América Latina sobre tasas de seroprevalencia y seroconversión en trabajadores médicos de la salud. La tasa relativamente alta (> 3%) de trabajadores de la salud asintomáticos con evidencia de infección previa por SARS-CoV-2 resalta la necesidad de realizar pruebas de detección de infección en esta población para prevenir la propagación de la infección.

Humans , Male , Female , Adult , Middle Aged , Health Personnel , Seroconversion , SARS-CoV-2 , Seroepidemiologic Studies , Prevalence , Risk Factors , Colombia , Delivery of Health Care , COVID-19 , Occupational Groups
Arq. gastroenterol ; 58(2): 150-156, Apr.-June 2021. tab, graf
Article in English | LILACS | ID: biblio-1285316


ABSTRACT BACKGROUND: Viral hepatitis is a global phenomenon, with the disease burden varying on a daily basis. Amongst chronic infections, hepatitis B virus and hepatitis C virus (HCV) are egregiously linked to severe health-related complications, with a worldwide prevalence of 248 million and 71 million respectively. Amongst the developing world, a hand full of countries are exhibiting a gross decline in chronic viral infection prevalence, like Bangladesh. While countries such as India have a consistent prevalence, Pakistan bears one of the largest proportions of chronic viral hepatitis globally with increasing trends shown year-by-year. Various old literature texts have stated an approximate national prevalence rate around 2.6% and 5.3% of hepatitis B and C respectively. OBJECTIVE: The objective of this study was to determine the current seroprevalence rates of chronic viral hepatitis amongst the general population of rural Sindh using a screening program to determine the current disease burden. METHODS: An observational, cross-sectional survey based on a screening program was conducted in 5 districts with a combined population of over 6.5 million. The screening was carried out via the administration of various camps with the assistance of local social workers and welfare organizations. A total of 24,322 individuals met the inclusion criteria and were screened through (HBsAg/HCV) rapid test cassette (WC) Imu-Med one-step diagnostic test. RESULTS: Hepatitis B was found positive in 964 (3.96%) individuals including 421 (43.67%) males and 543 (56.32%) females, while hepatitis C was positive in 2872 (11.80%) individuals including 1474 (51.32%) males and 1398 (48.67%) females. The prevalence amongst the districts varied between 0.97% and 9.06% for hepatitis B, and 1.61% and 29.50% for hepatitis C, respectively. Umerkot was found to be the most prevalent district amongst rural Sindh, while Badin had the least number of seropositive people. The second most prevalent district of the study population was found to be Tando Allahyar followed by Mirpur Khas. The combined seroprevalence of 15.76% was calculated for hepatitis B and C together amongst the five studied districts of rural and peri-urban Sindh. CONCLUSION: The alarmingly high prevalence rates revealed in our study warrant the urgent need to generate multiple effective strategies in the region to enhance awareness amongst the general population regarding screening, prevention, and prompt treatment of the disease.

RESUMO CONTEXTO: A hepatite viral é um fenômeno global, com a intensidade da doença variando diariamente. Entre as infecções crônicas, o vírus da hepatite B e o vírus da hepatite C (VHC) estão fortemente ligados a complicações graves relacionadas à saúde, com prevalência mundial de 248 milhões e 71 milhões, respectivamente. Entre o mundo em desenvolvimento, uma quantidade de países está exibindo um declínio bruto na prevalência de infecção viral crônica, tal como Bangladesh. Embora países como a Índia tenham uma prevalência consistente, o Paquistão tem uma das maiores proporções globais de hepatite viral crônica, com tendências crescentes mostradas ano a ano. Vários textos da menos recentes têm declarado uma taxa de prevalência nacional aproximada em torno de 2,6% e 5,3% da hepatite B e C, respectivamente. OBJETIVO: O objetivo deste estudo foi determinar as atuais taxas de soroprevalência da hepatite viral crônica entre a população geral do Sindh rural utilizando um programa de triagem para determinar a carga atual da doença. MÉTODOS: Foi realizada uma pesquisa observacional e transversal baseada em um programa de triagem combinada em cinco distritos com população de mais de 6,5 milhões. A triagem foi realizada por meio da administração de diversos acampamentos com o auxílio de assistentes sociais locais e organizações de assistência social. Um total de 24.322 indivíduos atenderam aos critérios de inclusão e foram examinados através do teste rápido (HBsAg/VHC) Imu-Med em uma etapa. RESULTADOS: Hepatite B positiva foi encontrada em 964 (3,96%) indivíduos incluindo 421 (43,67%) homens e 543 (56,32%) mulheres, enquanto hepatite C foi positiva em 2.872 (11,80%) indivíduos incluindo 1.474 (51,32%) homens e 1.398 (48,67%) mulheres. A prevalência entre os distritos variou entre 0,97% e 9,06% para hepatite B, e 1,61% e 29,50% para hepatite C, respectivamente. Umerkot foi encontrado como o distrito mais prevalente entre Sindh rural, enquanto Badin tinha o menor número de pessoas soropositivas. O segundo distrito mais prevalente da população de estudos foi encontrado como Tando Allahyar, seguido por Mirpur Khas. A soroprevalência combinada de 15,76% foi calculada para hepatite B e C em conjunto entre os cinco distritos estudados do Sindh rural e periurbano. CONCLUSÃO: As taxas de prevalência alarmantemente reveladas em nosso estudo justificam a necessidade urgente de gerar múltiplas estratégias efetivas na região para aumentar a conscientização da população em geral sobre rastreamento, prevenção e tratamento rápido da doença.

Humans , Male , Female , Hepatitis C/diagnosis , Hepatitis C/epidemiology , Hepatitis B/diagnosis , Hepatitis B/epidemiology , Pakistan/epidemiology , Seroepidemiologic Studies , Prevalence , Cross-Sectional Studies
Medicina (B.Aires) ; 81(2): 135-142, June 2021. graf
Article in English | LILACS | ID: biblio-1287262


Abstract Most countries in Latin America have already reported thousands of confirmed cases and vulnerable populations are the most affected by the coronavirus disease 2019 (COVID-19) pandemic. Preventive measures such as hygiene, social distancing, and isolation, essential to stop the spread of coronavirus, are difficult to accomplish for vulnerable populations due to their living conditions. Seroepidemiological surveys are assets to measure the transmission for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Until July 1st, the incidence rate of SARS-CoV-2 infection in Barrio Padre Mugica, one of the largest slums in Buenos Aires City, was 5.9%. This study aimed to establish the prevalence of SARS-CoV-2 antibodies immunoglobulin G (IgG) immediately after the outbreak, and to identify neighbourhood, household and individual factors associated with seroconversion. The prevalence based on IgG was 53.4% (95% CI 52.8% to 54.1%). For each polymerase chain reaction (RT-qPCR) confirmed case, nine people tested IgG positive, indicating a high rate of undetected (probably asymptomatic) infections. Hence, the high rate of undiagnosed people suggests that clinical criteria and epidemiological nexus should be considered. The high seroprevalence observed in the context of an intense epidemic in a vulnerable area might serve as a reference to other countries. This study contributes to future decision making by understanding population immunity against SARS-CoV2 and its relation to living conditions and foccus that comprehensive biosocial, household-level interventions are needed.

Resumen Muchos países de América Latina han informado miles de casos confirmados y las poblaciones vulnerables son las más afectadas por la pandemia de la enfermedad por coronavirus 2019 (COVID-19). Las medidas preventivas como la higiene, el distanciamiento social y el aislamiento, fundamentales para frenar la propagación del coronavirus, son difíciles de lograr en estas poblaciones debido a sus condiciones de vida. Los estudios seroepidemiológicos son de gran utilidad para medir la transmisión del síndrome respiratorio agudo severo coronavirus 2 (SARS-CoV-2). Hasta el 1 de julio, la tasa de incidencia de la infección por SARS-CoV-2 en el Barrio Padre Mugica, uno de los barrios marginales más grandes de la ciudad de Buenos Aires, era del 5.9%. Este estudio tuvo como objetivo estimar la prevalencia de anticuerpos inmunoglobulina G (IgG) para SARS-CoV-2 inmediatamente después del brote, e identificar factores del barrio, hogar e individuales asociados con la seroconversión. La prevalencia basada en IgG fue del 53.4% (IC del 95%: 52.8% a 54.1%). Para cada caso confirmado por reacción en cadena de la polimerasa (RT-qPCR), nueve personas dieron positivo en IgG, lo que indica una alta tasa de infecciones no detectadas y probablemente asintomáticas. La alta tasa de personas no diagnosticadas sugiere que se deben considerar los criterios clínicos y el nexo epidemiológico. La alta seroprevalencia observada en el contexto de una intensa epidemia en una zona vulnerable podría servir de referencia a otros países. Este estudio contribuye a la toma de decisiones futuras al comprender la inmunidad de la población contra el SARS-CoV2 en su relación con las condiciones de vida y por su enfoque en la necesidad de intervenciones integrales a nivel del hogar.

Humans , Poverty Areas , COVID-19 , RNA, Viral , Seroepidemiologic Studies , SARS-CoV-2 , Antibodies, Viral
Rev. bras. anal. clin ; 53(2): 167-174, 20210630. ilus, tab, graf
Article in Portuguese | LILACS | ID: biblio-1352925


A soroprevalência do SARS-CoV-2 foi determinada a partir de um inquérito epidemiológico transversal realizado no Hospital Universitário Gaffrée e Guinle, na cidade do Rio de Janeiro, Brasil, nos meses de outubro a novembro de 2020, durante a pandemia de COVID-19, na população de funcionários que exercia atividade laboral naquele período. A caracterização sociodemográfica foi realizada, além da análise das associações entre as variáveis de interesse ou exposição com a infecção pelo SARS-CoV-2, para estabelecer quais sinais e sintomas foram de maior prevalência. Para o estudo foram coletadas amostras de sangue e utilizados ensaios imunocromatográficos (COVID-19 IgG/IgM ECO Test® e Medtest® Coronavírus (COVID-19) IgG/IgM), para detectar IgG e IgM anti-SARS-CoV-2. Um cálculo amostral obtendo-se 391 profissionais de saúde demonstrou que 173 (44,2%) indivíduos apresentaram ao menos um resultado positivo para infecção pelo SARS-CoV-2. Não houve diferença significativa entre as medianas das idades dos pacientes que apresentaram infecção pelo SARS-CoV-2 (mediana 40 anos, IIQ 34-53) e dos que não apresentaram infecção (mediana 39 anos, IIQ 32-49), valor de P= 0,148. Também não houve diferença entre o sexo dos profissionais com infecção e sem infecção pelo SARS-CoV-2 (OR = 1,02; IC 95% 0,59 ­ 1,78). Dos 173 profissionais que apresentaram infecção pelo SARS-CoV-2, 70 (40,5%) relataram sintomas relacionados à COVID-19, enquanto 41 (23,7%) profissionais relataram que não apresentaram nenhum sintoma e 62 (35,8%) não informaram sobre presença ou não de sintomas, sendo excluídos da análise. Os sintomas mais frequentemente apresentados pelos profissionais infectados foram: cefaleia 46,5% (59/127); perda de olfato 32,3% (41/127); perda de paladar 30,7% (39/127); tosse seca 27,6% (35/127); diarreia 24,4% (31/127); dores no corpo 27,6% (35/127); outros 13,4% (17/127); dores nas pernas 11,8% (15/127); tonteira 11,8% (15/127) e febre 9,4% (12/127). Estabelecer um protocolo de vigilância com um teste fácil e rápido para as políticas de COVID-19 entre os profissionais na linha de frente e atuantes na assistência é imperativo para o controle da transmissão de SARS-CoV-2 e definir critérios epidemiológicos que orientem a tomada de decisão quanto ao isolamento de profissionais infectados sintomáticos e assintomáticos.

The seroprevalence of SARS-CoV-2 was determined from a cross-sectional epidemiological survey carried out at the Gaffrée and Guinle University Hospital in the city of Rio de Janeiro, Brazil from October to November 2020 during the COVID-19 pandemic in the population of employees who worked in that period. Sociodemographic characterization was performed in addition to the analysis of associations between the variables of interest or exposure with SARS-CoV-2 infection to establish which signs and symptoms were more prevalent. For the study, blood samples were collected and immunochromatographic assays (COVID-19 IgG/IgM ECO Test® and Medtest® Coronavirus (COVID-19) IgG/IgM) were used to detect IgG and IgM anti-SARS-CoV-2. A sample calculation obtaining 391 health professionals showed that 173 (44.2%) individuals had at least one positive result for SARS-CoV-2 infection. There was no significant difference between the median ages of patients who had SARS-CoV-2 infection (median 40 years, IIQ 34-53) and those who did not have infection (median 39 years, IIQ 32-49), P value = 0.148. There was also no difference between the sex of professionals with and without SARS-COV-2 infection (OR = 1.02; 95% CI 0.59 ­ 1.78). Of the 173 professionals who had SARS-COV-2 infection, 70 (40.5%) reported symptoms related to COVID-19, while 41 (23.7%) professionals reported that they had no symptoms and 62 (35.8%) did not report on the presence or absence of symptoms, being excluded from the analysis. The symptoms most frequently presented by the infected professionals were: headache 46.5% (59/127); loss of smell 32.3% (41/127); loss of taste 30.7% (39/127); dry cough 27.6% (35/127); diarrhea 24.4% (31/127); body pain 27.6% (35/127); another 13.4% (17/127); leg pain 11.8% (15/127); dizziness 11.8% (15/127) and fever 9.4% (12/127). Establish a surveillance protocol with an easy and quick test for COVID-19 policies among frontline and care professionals is imperative to control the transmission of SARS-CoV-2 and define epidemiological criteria that guide the decision-making regarding the isolation of symptomatic and asymptomatic infected professionals.

Humans , Male , Female , Signs and Symptoms , Seroepidemiologic Studies , Health Surveys , Health Personnel , COVID-19 Testing , SARS-CoV-2 , COVID-19/diagnosis
Rev. patol. trop ; 50(2): 1-15, jun. 2021. ilus
Article in English | LILACS | ID: biblio-1254525


Toxoplasmosis is a zoonosis caused by Toxoplasma gondii, a protozoan that has a cosmopolitan geographic distribution and low specificity for intermediate hosts. Domestic chickens are among the most important hosts in toxoplasmosis epidemiology, since they are potential sources of infection for humans, in addition to indicating environmental contamination by protozoa. In this work, we studied the prevalence of T. gondii infection in chickens in different breeding systems from distinct mesoregions of Rio Grande do Norte and Paraiba States: broiler chickens from commercial farms and free-range chickens from small farms. Indirect fluorescent antibody test (IFAT) and ELISA techniques were used for detecting specific antibodies in blood samples from the birds. There were no seropositive samples among the broilers tested, indicating that intensive management limited the chances of infection for these animals. Among the free-range chickens, the frequency of IgG anti-T. gondii detected by IFAT and ELISA were 37.9% and 40.4% respectively. Among the seropositive samples by IFAT, 33 (27.1%) were positive at a dilution of 1:16; in 1:32, 31 (25.4%); in 1:64, 24 (19.7%); 15 (12.3%) in 1:128, and 19 presented titers greater than or equal to 1:256 (15.6%). The substantial concordance observed between IFAT and ELISA signifies these methods are effective methodologies for the diagnosis of avian toxoplasmosis. The high prevalence of specific antibodies among poultry in the studied region highlights the potential risk of T. gondii transmission to humans through consumption of infected meat.

Humans , Animals , Seroepidemiologic Studies , Chickens , Toxoplasmosis , Infections
Rev. argent. salud publica ; 13: 1-11, 5/02/2021.
Article in Spanish | LILACS, BINACIS, ARGMSAL | ID: biblio-1343720


INTRODUCCIÓN: Los estudios de seroprevalencia permiten monitorear la circulación del SARS-CoV-2 y dan información para evaluar medidas sanitarias. El objetivo fue conocer la proporción y evolución de la seropositividad en puntos de gran circulación de la Ciudad Autónoma de Buenos Aires (CABA) y las características clínico-epidemiológicas de los seropositivos, de abril a octubre de 2020. MÉTODOS: Se realizó un estudio descriptivo transversal de seis rondas de testeos rápidos serológicos y una encuesta de datos epidemiológicos. Se realizó un muestreo por conveniencia en tres estaciones ferroviarias cabeceras de CABA consideradas puntos de alto tránsito bidireccional del Área Metropolitana de Buenos Aires. RESULTADOS: Participaron 7339 personas. La proporción de seropositivos fue 0,6% (IC95%: 0,2-0,9) en la primera ronda y aumentó a 5,6% en la última (IC95%: 4,3-7). Al inicio aumentó la seropositividad en residentes de CABA y de la zona sur de la Región Metropolitana de Buenos Aires. El antecedente de haber presentado síntomas y el de contacto con personas con COVID-19 fueron las únicas variables relacionadas con el resultado de inmunoglobulina G positivo (p <0,05). El 56,1% (n = 97) de los seropositivos no tuvo síntomas. El 78,4% (n = 134) no fue diagnosticado en la etapa aguda. DISCUSIÓN: La seropositividad fue en ascenso en cada ronda, en coincidencia con la situación epidemiológica de la zona de residencia. Las características epidemiológicas como la proporción de seropositivos sin antecedentes de síntomas, reafirman la importancia de las medidas sanitarias poblacionales.

Argentina , Epidemiologic Studies , Seroepidemiologic Studies , Coronavirus Infections , Antibodies, Viral
Rev. argent. salud publica ; 13: 1-9, 5/02/2021.
Article in Spanish | LILACS, BINACIS, ARGMSAL | ID: biblio-1150304


INTRODUCCIÓN: Citomegalovirus (CMV) es agente causal de infección congénita en todo el mundo con gran impacto socioeconómico, dado que produce pérdida de audición en la niñez y retraso del neurodesarrollo. No existen aún tratamientos con eficacia comprobada para todos los grupos de pacientes, ni se ha licenciado una vacuna segura y eficaz; tampoco se ha logrado consenso respecto al screening universal. Los objetivos de este trabajo fueron estimar la prevalencia de infección congénita por CMV y seroprevalencia materna en Tucumán y comparar si existen diferencias en las variables estudiadas según un índice de carencias y tipo de institución (pública o privada). MÉTODOS: Se realizó un estudio observacional de corte transversal. Se reclutaron 201 binomios recién nacido (RN)/madre de 8 maternidades del subsector público y privado. Se obtuvieron muestras de suero de la madre y orina del RN y se completó una Ficha de Recolección de Datos. Se utilizó PCR en tiempo real para la detección de genoma viral en muestras de orina de los RN y se realizó detección de IgG anti-CMV en el suero de las madres. RESULTADOS: Se obtuvo una prevalencia estimada de infección congénita por CMV de 0,5% y una seroprevalencia materna de 96,9%. Se encontraron diferencias según índice de carencias y tipo de institución. DISCUSIÓN: La frecuencia de infección congénita fue baja, comparada con la seroprevalencia materna. Algunas de las variables estudiadas evidencian mayor vulnerabilidad en el sector público

Urine , Seroepidemiologic Studies , Epidemiology , Cytomegalovirus Infections
Epidemiol. serv. saúde ; 30(1): e2020788, 2021. tab, graf
Article in English, Portuguese | LILACS, ColecionaSUS, SES-SP | ID: biblio-1142938


Objetivo: Analisar as notificações de síndrome gripal segundo o intervalo de tempo decorrido entre início dos sintomas e realização do exame para COVID-19. Métodos: Estudo transversal, utilizando registros de casos de síndrome gripal contendo resultados de testes diagnósticos da COVID-19 nas capitais brasileiras e no Distrito Federal, no sistema e-SUS Notifica, entre 1º/março/2020 e 18/agosto/2020. Comparou-se o intervalo de tempo entre início dos sintomas e realização do exame (teste ANOVA), classificando-o segundo a adequação/oportunidade do exame. Resultados: Entre 1.942.514 notificações, o tempo médio entre início dos sintomas e execução dos testes foi de 10,2 dias (±17,1). Entre testados, predominou o sexo feminino (55,1%), idade de 20-39 anos (43,8%) e região Sudeste (43,0%). O teste ELISA IgM foi realizado em tempo adequado para 58,8%; e o teste rápido-antígeno, em tempo inadequado para 68,0%. Conclusão: Observou-se inadequação entre início dos sintomas e realização dos testes para COVID-19 nas regiões brasileiras.

Objetivo: Analizar las notificaciones de síndrome gripal según el intervalo de tiempo entre el inicio de los síntomas y el examen de COVID-19. Métodos: Estudio transversal utilizando registros de casos de síndrome gripal que contienen resultados de pruebas diagnósticas de COVID-19 en las capitales brasileñas y el Distrito Federal del sistema e-SUS Notifica, entre 1/marzo/2020 y 18/agosto/2020. El intervalo de tiempo se comparó entre el inicio de los síntomas y la realización del examen mediante la prueba ANOVA, clasificándolo según la adecuación/ oportunidad del examen. Resultados: Entre 1.942.514 notificaciones, el tiempo promedio entre el inicio de los síntomas y la ejecución del examen fue de 10,2 días (±17,1). Entre los evaluados, predominaron las mujeres (55,1%), 20-39 años (43,8%) y la región Sudeste (43,0%). El ELISA IgM se realizó en momento adecuado para 58,8% y la prueba de Antígeno Rápido en momento inadecuado para 68,0%. Conclusión: Se constata inadecuación de tiempo entre el inicio de los síntomas y las pruebas para COVID-19 en las regiones brasileñas.

Objective: To analyze notifications of flu-like syndrome according to the time interval between onset of symptoms and testing for COVID-19. Methods: This was a cross-sectional study using records of flu-like syndrome cases containing results of COVID-19 diagnostic tests in the Brazilian state capitals and Federal District, held on the e-SUS Notifica system, from March 1st, 2020 to August 18th, 2020. The time interval between symptom onset and testing was compared using the ANOVA test, classifying it according to test adequacy/timeliness. Results: Taking 1,942,514 notifications, average time between symptom onset and testing was 10.2 days (±17.1). Among those tested, females (55.1%), people aged 20-39 years (43.8%), and the Southeast region of Brazil (43.0%) predominated. 58.8% of IgM ELISA tests were performed at an adequate time while 68.0% of rapid antigen tests were not performed at an adequate time. Conclusion: Inadequacy was found between symptom onset and time taken to test for COVID-19 in the Brazilian regions.

Humans , Serologic Tests/statistics & numerical data , Seroepidemiologic Studies , Coronavirus Infections/diagnosis , Coronavirus Infections/epidemiology , Laboratory Test , Brazil/epidemiology , Enzyme-Linked Immunosorbent Assay/statistics & numerical data , Cross-Sectional Studies , Public Health Surveillance
Actual. SIDA. infectol ; 29(107): 144-149, 2021 nov. fig
Article in Spanish | LILACS | ID: biblio-1348768


Se desconoce si las personas que viven con el VIH (PVV) tienen un mayor riesgo de contraer la infección por SARS-CoV-2. Los estudios serológicos prospectivos pueden permitir análisis de seroincidencia. Este estudio prospectivo incluyó a PVV sin antecedentes de vacunación contra SARS-CoV-2 de la Ciudad Autónoma de Buenos Aires (CABA) y Gran Buenos Aires (GBA) que concurrieron entre marzo y junio de 2021 a realizar sus controles al instituto INBIRS. La tasa de seroprevalencia de anticuerpos contra la proteína espiga del SARS-CoV-2 fue del 33,3% (IC 24,9-42,5%) para la población estudiada. Esta prevalencia fue significativamente mayor a la reportada previamente para la población general de la misma región geográfica. No se observó una asociación entre el recuento de linfocitos T CD4+ con los niveles de IgG específica. En conclusión, el hallazgo de una alta seroprevalencia de anticuerpos contra SARS-CoV-2 entre las PVV en CABA y GBA puede sugerir una mayor susceptibilidad a la infección por este virus; sin embargo, puede ser también un marcador subrogante que indica la tasa de seroprevalencia en población general se encuentra subestimada

It is not known whether people living with HIV (PLHIV) are at increased risk of acquiring SARS-CoV-2 infection. Prospective serological studies can allow seroincidence analysis. This prospective study included PLHIV, without a history of vaccination against SARS-CoV-2, from the Autonomous City of Buenos Aires (CABA) and Buenos Aires surroundings (GBA), who attended INBIRS Institute between March and June 2021 to carry out their controls. The seroprevalence rate of antibodies against the SARS-CoV-2 spike protein was 33.3% (CI 24.9-42.5%) for the study population. This prevalence was significantly higher than that previously reported for the general population of the same geographic region. No association was found between CD4+ T-cell counts with levels of SARS-CoV-2 specific IgG. In conclusion, the finding of a high seroprevalence of antibodies against SARS-CoV-2 among PLHIV in CABA and GBA may suggest a greater susceptibility to infection; however, it can also be a surrogate marker that the seroprevalence rate in the general population is underestimated.

Humans , Adult , Middle Aged , Seroepidemiologic Studies , Cross-Sectional Studies/statistics & numerical data , Anti-Retroviral Agents/immunology , SARS-CoV-2/immunology , COVID-19/immunology
Braz. J. Vet. Res. Anim. Sci. (Online) ; 58: e180127, 2021. tab, mapas
Article in English | ID: biblio-1344687


The epidemiological characteristics of bovine leptospirosis in animals and herds in Mato Grosso do Sul were investigated to determine parameters such as disease frequency and the serovars reactant in beef cattle herds. A total of 4,629 beef cattle herds were examined against 33 Leptospira spp. serovars. The serum samples were submitted to the microscopic agglutination test (MAT) for the serological diagnosis of leptospirosis. The MAT results showed that 3,814 (82.39%) of the 4,629 animals evaluated were seropositive for the bacterium, with serological reactions mainly to serogroup Sejroe, serovar Wolffi (36.49%). The observed high frequency of reactive animals demonstrates the relevance of the infection. Therefore, general and specific measures should be implemented to contain and/or prevent infection of the animals in the studied region.(AU)

Foi realizado um inquérito epidemiológico da leptospirose em bovinos de rebanhos de corte do estado de Mato Grosso do Sul, de modo a determinar a frequência e as sorovariedades reagentes. Para isso, foram examinados 4.629 bovinos de corte, com uma coleção de 33 sorovariedades de Leptospira, por meio da prova de Soroaglutinação Microscópica (MAT). Dos 4.629 animais examinados, 3.814 (82,39%) foram reagentes com reações predominates para o sorogrupo Sejroe, sorovar Wolffi (36,49%). Assim, a alta frequência de animais reagentes encontrada justifica a implantação de medidas gerais e específicas para conter e/ou prevenir a infecção nos animais dessa região.(AU)

Animals , Cattle , Cattle/microbiology , Seroepidemiologic Studies , Leptospirosis/epidemiology
Rev. Soc. Bras. Med. Trop ; 54: e0853-2020, 2021. tab
Article in English | LILACS | ID: biblio-1155565


Abstract INTRODUCTION: Brazil ranks first in the number of HTLV-1/-2-infected individuals worldwide. The high morbidity and mortality of HTLV-1-associated diseases, especially following infection in infancy, requires strong action to reduce vertical transmission. METHODS: To facilitate the appraisal of the implementation of the HTLV antenatal screening program by the Brazilian Ministry of Health, we determined the costs in distinct scenarios according to HTLV seroprevalence, specificity of the screening test, and type of confirmatory test. RESULTS: HTLV antenatal screening would cost R$ 55,777,012-R$ 77,082,123/year. Screening assays with high specificity reduce the need and cost of confirmatory assays by up to 25%. CONCLUSIONS: Careful selection of the screening assay is required to optimize the program.

Humans , Female , Human T-lymphotropic virus 1 , HTLV-I Infections/diagnosis , HTLV-II Infections/diagnosis , Prenatal Diagnosis , Brazil , Seroepidemiologic Studies , Delivery of Health Care
Rev. Soc. Bras. Med. Trop ; 54: e0247-2020, 2021. tab, graf
Article in English | LILACS | ID: biblio-1155527


Abstract INTRODUCTION: This study estimated the seroprevalence and risk factors of Chagas disease (CD) in a population of the Quixeré municipality, Ceará. METHODS: We conducted serological methods to detect the Trypanosoma cruzi infection. The other variables were evaluated by a standardized questionnaire. RESULTS: The estimated prevalence of CD was 3.7%. Male sex, age >40 years, being farmers, low education level, origin from rural areas, and being born in Quixeré were significantly associated with infection. CONCLUSION: CD persists in this rural population of Northeast Brazil. Poverty, low education, and limited information regarding CD are critical issues that need to be addressed.

Humans , Male , Adult , Trypanosoma cruzi , Chagas Disease/epidemiology , Rural Population , Brazil/epidemiology , Seroepidemiologic Studies , Prevalence , Risk Factors
Rev. panam. salud pública ; 45: e66, 2021. tab, graf
Article in English | LILACS | ID: biblio-1280511


ABSTRACT Objective. To evaluate the seroprevalence of COVID-19 infection in pauci-symptomatic and asymptomatic people, the associated epidemiological factors, and IgG antibody kinetic over a 5-month period to get a better knowledge of the disease transmissibility and the rate of susceptible persons that might be infected. Methods. Seroprevalence was evaluated by a cross-sectional study based on the general population of Santa Fe, Argentina (non-probabilistic sample) carried out between July and November 2020. A subgroup of 20 seropositive individuals was followed-up to analyze IgG persistence. For the IgG anti-SARS-CoV-2 antibodies detection, the COVID-AR IgG® ELISA kit was used. Results. 3 000 individuals were included conforming asymptomatic and pauci-symptomatic groups (n=1 500 each). From the total sample, only 8.83% (n=265) presented reactivity for IgG anti-SARS-CoV-2. A significant association was observed between positive anti-SARS-CoV-2 IgG and a history of contact with a confirmed case; the transmission rate within households was approximately 30%. In the pauci-symptomatic group, among the seropositive ones, anosmia and fever presented an OR of 16.8 (95% CI 9.5-29.8) and 2.7 (95% CI 1.6-4.6), respectively (p <0.001). In asymptomatic patients, IgG levels were lower compared to pauci-symptomatic patients, tending to decline after 4 months since the symptoms onset. Conclusion. We observed a low seroprevalence, suggestive of a large population susceptible to the infection. Anosmia and fever were independent significant predictors for seropositivity. Asymptomatic patients showed lower levels of antibodies during the 5-month follow-up. IgG antibodies tended to decrease over the end of this period regardless of symptoms.

RESUMEN Objetivo. Evaluar la seroprevalencia de la infección por el virus causante de la COVID-19 en personas paucisintomáticas y asintomáticas, los factores epidemiológicos asociados y la cinética de los anticuerpos IgG durante un período de cinco meses para conocer mejor la transmisibilidad de la enfermedad y la tasa de personas susceptibles a estar infectadas. Métodos. Se evaluó la seroprevalencia mediante un estudio transversal basado en la población general de Santa Fe, Argentina (muestra no probabilística) llevado a cabo entre julio y noviembre del 2020. Se realizó un seguimiento de un subgrupo de 20 personas seropositivas para analizar la persistencia de los anticuerpos IgG. Para la detección de los anticuerpos IgG contra SARS-COV-2, se empleó el kit ELISA COVID-AR IgG®. Resultados. Hubo 3 000 participantes divididos en un grupo asintomático y un grupo paucisintomático (n=1 500 cada grupo). De la muestra total, solo 8,83% (n=265) presentó una reactividad de IgG contra el SARS-CoV-2. Se observó una asociación significativa entre anticuerpos IgG positivos contra el SARS-CoV-2 y antecedente de contacto con un caso confirmado. La tasa de transmisión en el hogar fue de 30% aproximadamente. En el grupo paucisintomático, entre las personas seropositivas, la anosmia y la fiebre presentaron un OR de 16,8 (IC 95% 9,5-29,8) y 2,7 (IC 95% 1,6-4,6), respectivamente (p <0,001). En los pacientes asintomáticos, los niveles de IgG fueron inferiores en comparación con los pacientes paucisintomáticos, con tendencia a la baja pasados cuatro meses desde la aparición de los síntomas. Conclusiones. Se observó una seroprevalencia baja, indicadora de una gran población susceptible a la infección. La anosmia y la fiebre fueron factores predictivos independientes de relevancia para la seropositividad. Los pacientes asintomáticos mostraron niveles inferiores de anticuerpos durante el seguimiento de cinco meses. Los anticuerpos IgG tendieron a disminuir hacia el final del período con independencia de los síntomas.

RESUMO Objetivo. Avaliar a soroprevalência de anticorpos contra a COVID-19 em indivíduos paucissintomáticos e assintomáticos, os fatores epidemiológicos associados e a cinética dos anticorpos da classe IgG em um período de 5 meses, visando aprimorar o conhecimento sobre a transmissibilidade da doença e a taxa de suscetíveis à infecção. Métodos. Inquérito transversal de soroprevalência realizado na população geral (amostra não probabilística) de Santa Fé, na Argentina, entre julho e novembro de 2020. Um subgrupo de 20 indivíduos soropositivos foi acompanhado para analisar a persistência de anticorpos IgG. O kit de ensaio imunoenzimático (ELISA) COVID-AR IgG® foi usado para a detecção de anticorpos IgG contra SARS-CoV-2. Resultados. A amostra compreendeu 3 000 indivíduos, divididos entre assintomáticos e paucissintomáticos (n = 1.500 por grupo). Deste total, somente 8,83% (n = 265) apresentaram reatividade, com a detecção de anticorpos IgG contra SARS-CoV-2. Observou-se uma associação significativa entre a presença de anticorpos IgG contra SARS-CoV-2 e histórico de contato com caso confirmado. A taxa de transmissão intradomiciliar foi de aproximadamente 30%. No grupo paucissintomático, entre os soropositivos, o odds ratio (OR) para anosmia foi de 16,8 (IC 95% 9,5-29,8), e para febre, 2,7 (IC 95% 1,6-4,6) (p <0,001). Os indivíduos assintomáticos apresentaram níveis de IgG mais baixos que os paucissintomáticos, com uma tendência de declínio após 4 meses do início dos sintomas. Conclusões. Observou-se uma soroprevalência baixa de anticorpos contra a COVID-19 na população estudada, o que indica um grande número de pessoas suscetíveis à infecção. Anosmia e febre foram preditores importantes independentes de soropositividade. Os assintomáticos apresentaram níveis mais baixos de anticorpos aos 5 meses de acompanhamento. Houve uma tendência de redução dos anticorpos IgG ao final deste período, independentemente da presença de sintomas.

Humans , Male , Female , Adolescent , Adult , Middle Aged , Aged , Carrier State/epidemiology , COVID-19/diagnosis , COVID-19/epidemiology , Argentina/epidemiology , Immunoglobulin G/blood , Enzyme-Linked Immunosorbent Assay , Seroepidemiologic Studies , Cross-Sectional Studies , COVID-19 Serological Testing , Anosmia/virology
Rev. cuba. med. trop ; 72(3): e540, sept.-dic. 2020. tab
Article in Spanish | LILACS, CUMED | ID: biblio-1156534


Introducción: La leptospirosis es una enfermedad infecciosa bacteriana causada por leptospiras patógenas, que afectan a una amplia variedad de mamíferos incluido el hombre. Es considerada como una de las enfermedades zoonóticas de mayor distribución a nivel mundial. Objetivo: Determinar la seroprevalencia de anticuerpos contra Leptospira spp. y factores de riesgo asociados a la leptospirosis en algunas poblaciones de cerdos de Portoviejo. Métodos: Estudio descriptivo, realizado entre octubre de 2018 a marzo de 2019. Se estudiaron cerdos criados en parroquias del cantón Portoviejo, Manabí, Ecuador. Se colectaron un total de 280 muestras de sangre (115 de cerdos en crianza tecnificada y 165 en crianza de traspatio), 140 en el periodo lluvioso (enero-marzo) y 140 en el periodo seco (octubre-diciembre), de forma tal que fueran representativos los animales para ambas épocas. El diagnóstico serológico se realizó mediante la prueba de aglutinación microscópica. Resultados: En la crianza tecnificada, la seroprevalencia fue de 16,52 por ciento (19/115) y en la crianza de traspatio, 20,61 por ciento (34/165). Se presentó con mayor frecuencia en serovares Australis e Icterohaemorrhagiae en 14,28 por ciento (40/280), respectivamente, y Bataviae en 13,21 por ciento (37/280). El riesgo asociado a la infección por leptospiras fue mayor en los cerdos del sexo femenino, en las poblaciones de cerdos que consumieron agua de ríos y en los predios donde no se realizó un control de roedores silvestres. Conclusiones: La seroprevalencia a anticuerpos contra Leptospira spp. en la población de cerdos en crianza tecnificada y traspatio fue moderada, pero constituye un peligro potencial para la población humana expuesta. En ambos sistemas de crianza, los serovares más frecuentes fueron Australis, Icterohaemorrhagiae y Bataviae(AU)

Introduction: Leptospirosis is an infectious disease caused by pathogenic Leptospira sp. bacteria. It affects a large number of mammals, including humans, and is considered to be one of the most widely distributed zoonotic diseases worldwide. Objective: Determine the seroprevalence of antibodies against Leptospira spp. and the risk factors associated to leptospirosis in some pig populations from Portoviejo. Methods: A descriptive study was conducted from October 2018 to March 2019 of pigs raised in parishes from Portoviejo Canton in Manabí, Ecuador. A total 280 blood samples were collected (115 from pigs raised technically and 165 from pigs raised in backyards), 140 in the rainy season (January-March) and 140 in the dry season (October-December), so that the samples were representative of the two seasons. Serological diagnosis was performed with the microscopic agglutination test. Results: In technical breeding, seroprevalence was 16.52 percent (19/115), whereas in backyard breeding it was 20.61 percent (34/165). It was most common in serovars Australis and Icterohaemorrhagiae, with 14.28 percent (40/280), respectively, and Bataviae with 13.21 percent (37/280). Risk associated to Leptospira infection was greater in sows, in pig populations which drank river water, and in areas where wild rodent control was not present. Conclusions: Seroprevalence of antibodies against Leptospira spp. was moderate in technical and backyard pig raising populations, but it is a potential hazard for the human population exposed. In both breeding systems, the most common serovars were Australis, Icterohaemorrhagiae and Bataviae(AU)

Animals , Swine , Seroepidemiologic Studies , Leptospirosis/diagnosis , Antibodies , Epidemiology, Descriptive , Ecuador
Rev. Hosp. Ital. B. Aires (2004) ; 40(4): 233-236, dic. 2020. ilus
Article in Spanish | LILACS | ID: biblio-1145604


Entre el 1 y el 26 de junio se llevó a cabo el estudio de investigación "Encuesta de infección por coronavirus tipo 2 del síndrome respiratorio agudo grave (SARS-CoV-2), nivel comunitario en habitantes de un barrio vulnerable urbano de la ciudad de Buenos Aires", que determinó que un 54,3% de los habitantes del barrio presentaban anticuerpos inmunoglobulina tipo G para SARS-CoV-2. El objetivo de este artículo es proporcionar un ejemplo de un muestreo probabilístico que fue utilizado para estimar la prevalencia de seropositividad en este estudio. (AU)

Between 1st and 26th of june, a research named "Survey of infection by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), community level in inhabitants of a marginal urban neighborhood of the city of Buenos Aires" was carried on. The study showed that 54.3% of the that 54.3% of the people of the neighborhood had antibodies immunoglobulin type G for SARS-CoV-2. The objective of this article is to provide an example of a probability sampling carried out in the study, to measuring the prevalence of seropositivity. (AU)

Humans , Pneumonia, Viral/epidemiology , Health Surveys/statistics & numerical data , Coronavirus Infections/epidemiology , Argentina , Pneumonia, Viral/immunology , Seroepidemiologic Studies , Simple Random Sampling , Prevalence , Coronavirus Infections/immunology , Betacoronavirus/immunology
Arch. argent. pediatr ; 118(6): 381-385, dic 2020. tab
Article in English, Spanish | LILACS, BINACIS | ID: biblio-1146048


Introducción. En pocos meses, la enfermedad por coronavirus 2019 (COVID-19) se transformó en una pandemia, alcanzando millones de casos y centenas de miles de muertos. Por la enorme capacidad de contagio, el personal de salud se encuentra más expuesto a contraerla. Sin embargo, aún hay muy poca información al respecto, particularmente, entre pediatras.Objetivo. Evaluar la seroprevalencia de anticuerpos anti SARS-CoV-2 en médicos de un hospital pediátrico, a 3 meses del primer caso en la Argentina. Explorar si algunas características demográficas, profesionales y epidemiológicas son factores de riesgo para presentar seropositividad para SARS-CoV-2.Métodos. Estudio transversal, con médicos seleccionados aleatoriamente de la nómina de un hospital pediátrico. En todos, se pesquisaron anticuerpos anti SARS-CoV-2por quimioluminiscencia (inmunoglobulinas G/M -IgG/IgM-) y se recogieron antecedentes epidemiológicos (edad, lugar de residencia, categoría profesional, contacto con enfermos), para explorar asociación entre los predictores y seropositividad.Resultados. Se incluyeron 116 médicos, con una edad promedio de 45,6 ± 13,3 años. El 62,9 % eran médicos de planta, y el resto, residentes. El 42,2 % prestaban servicios en áreas dedicadas a la asistencia directa de pacientes con COVID-19.Cuatro (el 3,5 %) refirieron contacto con enfermos sin la debida protección, y 6 (el 5,2 %), viaje previo a zonas de riesgo. Solo se identificaron anticuerpos anti-SARS-CoV-2 (IgG) en un profesional. Debido a ello, no se pudo evaluar asociación entre los potenciales predictores y la presencia de anticuerpos anti-SARS-CoV-2.Conclusión. Se encontró que solo el 0,9 % de los médicos de un hospital pediátrico presentaban anticuerpos anti-SARS-CoV-2.

Introduction. In just a few months, coronavirus disease 2019 (COVID-19) has become a pandemic, causing millions of cases and hundreds of thousands of deaths. Due to its high infectiousness, the health care staff is even more exposed. Nevertheless, there is still very limited information about it, especially among pediatricians.Objective. To assess the seroprevalence of SARS-CoV-2 antibodies among physicians from a children's hospital 3 months after the first case in Argentina. To explore if certain demographic, occupational, and epidemiological characteristics are risk factors for SARS-CoV-2 seropositivity.Methods. Cross-sectional study of physicians randomly selected from the list of workers of a children's hospital. All of them were screened for SARS-CoV-2 antibodies by chemiluminescence (immunoglobulins G/M [IgG/IgM]) and their epidemiological history was collected (age, place of residence, professional category, contact with infected people) in order to explore the association between predictors and seropositivity.Results. A total of 116 physicians were included; their average age was 45.6 ± 13.3 years. Of them, 62.9 % were staff physicians, and the rest, residents; 42.2 % worked in areas designated for direct care of COVID-19 patients. Four (3.5 %) referred contact with infected people without the appropriate protection, and 6 (5.2 %), having traveled to risk areas. SARS-CoV-2 antibodies (IgG) were only detected in one of the professionals. Because of this, it was impossible to assess the association between potential predictors and the presence of SARS-CoV-2 antibodies.Conclusion. It was observed that only 0.9 % of the physicians from a children's hospital had SARS-CoV-2 antibodies

Humans , Male , Female , Adult , Middle Aged , Aged , Physicians/statistics & numerical data , Coronavirus Infections/immunology , Betacoronavirus/immunology , Seroepidemiologic Studies , Epidemiology, Descriptive , Cross-Sectional Studies , Risk Factors , Hospitals, Pediatric , Luminescent Measurements
Arq. bras. med. vet. zootec. (Online) ; 72(5): 1698-1704, Sept.-Oct. 2020. tab
Article in English | ID: biblio-1131578


The present study was carried out to evaluate the prevalence and hematological effects of Toxoplasma gondii in sheep and goat in district Dera Ghazi Khan. Blood samples (n=204) were collected comprise goats (n=101) and sheep (n=103) alongwith age, gender and breeds of animals. Samples were collected randomly from 25 flocks of 7 different union council Viz. Vehova, Tibbi Qaisrani, Lakhani, Kohar, Tuman Qaisrani, Nutkani and Kot Qaisrani of Tehsil Taunsa Sharif at least 4 animals from each flock. All ruminants divide into three groups based on age, breed and gender. The prevalence was detected through two different kits Viz. LAT and ELISA kit. The overall prevalence suspected in goats through LAT and ELISA kit was (35.64%), (32.67%) and in sheep was (25.24%), (23.30%) respectively. The Toxoplasma gondii had a significant effect on goats in age groups and non-significant all other groups of goats and sheep. Toxoplasma gondii had a significant effect on all hematological parameters like Hemoglobin, total leukocyte cells, granulocytes, lymphocytes, platelets, and red blood cells, except monocytes. In conclusion of the current study, toxoplasmosis is prevalent among ruminants, reveals the possibility of transmission to humans on the use of host animals as protein source.(AU)

O objetivo do presente estudo foi avaliar a prevalência e efeitos hematológicos de Toxoplasma gondii em ovelhas e cabras no distrito Dera Ghazi Khan. Amostras de sangue (n=204) foram coletadas para incluir cabras (n=101) e ovelhas (n=103), além de idade, gênero e raça dos animais. Amostras foram coletadas aleatoriamente de 25 rebanhos de 7 conselhos sindicais: Vehova, Tibbi Qaisrani, Lakhani, Kohar, Tuman Qaisrani, Nutkani e Kot Qaisrani of Tehsil Taunsa Sharif com pelo menos 4 animais em cada rebanho. Todos os ruminantes foram divididos em três grupos baseados em idade, raça e gênero. A prevalência foi detectada usando dois kits, LAT e ELISA. A prevalência total suspeita em cabras através dos kits LAT e ELISA foi (35.64%), (32.67%) e em ovelhas foi (25.24%), (23.30%) respectivamente. O Toxoplasma gondii teve efeito significativo em cabras em grupos de idade e não significativo em todos os outros grupos de cabras e ovelhas. Toxoplasma gondii teve efeito significativo em todos os parâmetros hematológicos como hemoglobina, células totais de leucócitos, granulócitos, linfócitos, plaquetas e glóbulos vermelhos, exceto monócitos. O presente estudo conclui que toxoplasmose é prevalente entre ruminantes, e revela a possibilidade de transmissão para humanos com o uso de animais hospedeiros como fonte de proteína.(AU)

Animals , Toxoplasma/isolation & purification , Goats/parasitology , Toxoplasmosis, Animal/epidemiology , Pakistan , Ruminants/parasitology , Enzyme-Linked Immunosorbent Assay/veterinary , Seroepidemiologic Studies , Prevalence
Arq. bras. med. vet. zootec. (Online) ; 72(4): 1197-1205, July-Aug. 2020. tab, mapas
Article in Portuguese | ID: biblio-1131503


A leishmaniose visceral americana (LVA) é uma zoonose de transmissão vetorial na qual o cão tem papel importante na epidemiologia da doença. No Brasil, a elevada prevalência da infecção em cães está diretamente correlacionada com o aumento no risco de ocorrência de casos de LVA. O objetivo deste estudo foi investigar a fauna flebotomínica e verificar a soroprevalência da leishmaniose visceral canina (LVC) na localidade Pedra 90, no município de Cuiabá. Para o levantamento entomológico, armadilhas CDC foram utilizadas de agosto de 2014 a julho de 2015. Na avaliação sorológica dos cães, o teste imunocromatográfico DPP LVC foi utilizado para a triagem das amostras, enquanto o ensaio imunoenzimático (EIE) para o diagnóstico da LVC (Bio-Manguinhos) foi empregado como teste confirmatório. O trabalho vem acrescentar à fauna flebotomínica do município de Cuiabá as espécies Lu. andersoni, Lu. braziliensis, Lu. bourrouli e Lu. scaffi, não registradas em publicações anteriores. Além disso, entre as espécies de flebotomíneos com importância médica, Lu. cruzi, Lu. flaviscutellata e Lu. whitmani foram capturadas. No inquérito canino, a prevalência de LVC observada na localidade Pedra 90 foi de 1,14%, indicando que a região pode ser considerada como área de transmissão.(AU)

American visceral leishmaniasis (AVL) is a vector-borne zoonosis in which the dog has an important role in the epidemiology of the disease. In Brazil, a high prevalence of canine infection is directly correlated with an increased risk of occurrence of AVL. The aim of this study was to investigate the phlebotomine fauna and seroprevalence of canine visceral leishmaniasis in Pedra 90 region of Cuiabá municipality. For the entomological survey, CDC traps were used from August 2014 to July 2015. In the serological evaluation of dogs, the immunochromatographic test DPP LVC was employed for screening the samples while enzyme-linked immunosorbent assay (Bio-Manguinhos) was used as a confirmatory assay. The previously unreported phlebotomine species Lu. andersoni, Lu. braziliensis, Lu. bourrouli, and Lu. scaffi were added to the phlebotomine fauna of Cuiabá. In addition, the medically important phlebotomine species Lu. cruzi, Lu. flaviscutellata, and Lu. whitmani were identified. The canine survey revealed the prevalence of 1.14% for canine visceral leishmaniasis in the Pedra 90 region, the region being considered a transmission area.(AU)

Animals , Dogs , Phlebotomus , Leishmaniasis, Visceral/epidemiology , Brazil , Seroepidemiologic Studies , Prevalence , Immunoenzyme Techniques/veterinary , Disease Transmission, Infectious/veterinary , Urban Area , Communicable Diseases, Emerging/veterinary
Rev. cuba. med. trop ; 72(2): e466, mayo.-ago. 2020. tab
Article in Spanish | LILACS, CUMED | ID: biblio-1149914


Introducción: La leptospirosis humana es una enfermedad zoonótica con manifestaciones clínicas inespecíficas (cefalea y mialgia), las cuales pueden confundirse con otras enfermedades febriles como: gripe, malaria, dengue, fiebre amarilla, hepatitis vírica y rickettsiosis. Objetivo: Determinar la prevalencia de anticuerpos anti-Leptospira en estudiantes de la carrera de Medicina Veterinaria y Zootecnia de la Costa Grande de Guerrero. Métodos: Se aplicó un cuestionario a los estudiantes de la Escuela Superior de Medicina Veterinaria y Zootecnia No. 3 con la finalidad de conocer algunos factores de riesgo asociados a la presencia de anticuerpos anti-Leptospira spp. La determinación de anticuerpos anti-Leptospira y la identificación de las serovariedades se realizaron con la técnica de microaglutinación. Se consideró un suero positivo cuando se observó una aglutinación en una dilución igual o mayor a 1:100. Resultados: En el estudio la seroprevalencia de anticuerpos anti-Leptospira spp. fue de 12,1 por ciento (8/66). Se detectaron anticuerpos a 8 serovariedades de Leptospira spp. El 62,5 por ciento (5/8) de los sueros presentó más de una serovariedad. Las serovariedades y frecuencia fueron icterohaemorrhagiae 25 por ciento (4/16), hardjo 18,8 por ciento (3/16), pyrogenes 18,8 por ciento (3/16), pomona 12,5 por ciento (2/16), canicola 6,2 por ciento (1/16) wolffi 6,2 por ciento (1/16) tarassovi 6,2 por ciento (1/16) y bratislava 6,2 por ciento (1/16). Los factores de riesgo estudiados no se relacionaron con la presencia de anticuerpos anti-Leptospira. Conclusiones: En el presente estudio se encontraron anticuerpos a Leptospira spp. en estudiantes de Medicina Veterinaria y Zootecnia. Por tanto, es pertinente proporcionar información a los estudiantes para prevenir el contacto con el agente causal de la leptospirosis(AU)

Introduction: Human leptospirosis is a zoonotic disease of unspecific clinical manifestations (headache and myalgia) which may be confused with those of other febrile diseases, such as influenza, malaria, dengue, yellow fever, viral hepatitis and rickettsiosis. Objective: Determine the prevalence of anti-Leptospira antibodies among Veterinary Medicine and Zootechnics students from Costa Grande de Guerrero. Methods: A questionnaire was applied to students from the Higher Education Veterinary Medicine and Zootechnics School No. 3 to obtain information about risk factors associated to the presence of anti-Leptospira spp. antibodies. Microagglutination technique was used to determine anti-Leptospira antibodies and identify serovars. A serum sample was considered positive when agglutination was observed at a dilution equal to or greater than 1:100. Results: Seroprevalence of anti-Leptospira spp. antibodies was found to be 12.1 percent (8/66). Antibodies were detected for 8 serovars of Leptospira spp. Of the serum samples analyzed, 62.5 percent (5/8) contained more than one serovar. The serovars and their frequency were the following: icterohaemorrhagiae 25 percent (4/16), hardjo 18.8 percent (3/16), pyrogenes 18.8 percent (3/16), pomona 12.5 percent (2/16), canicola 6.2 percent (1/16), wolffi 6.2 percent (1/16), tarassovi 6.2 percent (1/16) and bratislava 6.2 percent (1/16). The risk factors studied were not related to the presence of anti-Leptospira antibodies. Conclusions: Leptospira spp. antibodies were found in Veterinary Medicine and Zootechnics students. It is therefore advisable to provide students with the information required to prevent contact with the causative agent of leptospirosis(AU)

Humans , Male , Female , Young Adult , Leptospirosis/epidemiology , Antibodies/analysis , Schools, Veterinary , Veterinary Medicine , Seroepidemiologic Studies , Cross-Sectional Studies