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Article de Anglais | IMSEAR | ID: sea-35942

RÉSUMÉ

Enteropathogenic Escherichia coli (EPEC) is a leading cause of diarrhea among infants in developing countries. A total of 38 EPEC isolates, obtained from diarrhea patients of Hospital Miri, Sarawak, were investigated through plasmid profile, antibiotic resistance and randomly amplified polymorphic DNA (RAPD) analysis. From the 8 types of antibiotics used, all isolates were 100% resistant to furoxime, cephalothin and sulphamethoxazole and showed high multiple antibiotic resistant (MAR) indexes, ranging from 0.5 to 1.0. In plasmid profiling, 22 isolates (58%) showed the presence of one or more plasmids in the range 1.0 to 30.9 mDa. The dendrogram obtained from the results of the RAPD-PCR discriminated the isolates into 30 single isolates and 3 clusters at the level of 40% similarity. The EPEC isolates were highly diverse, as shown by their differing plasmid profiles, antibiotic resistance patterns and RAPD profiles.


Sujet(s)
Adulte , Profilage d'ADN/méthodes , Diarrhée/microbiologie , Résistance aux substances/génétique , Escherichia coli/classification , Infections à Escherichia coli/épidémiologie , Humains , Malaisie/épidémiologie , Tests de sensibilité microbienne , Plasmides/génétique , Valeur prédictive des tests , Technique RAPD
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