Influence of thermochemotherapy on the activity of cytotoxic T lymphocyte in oral maxillofacial cancer patients / 华西口腔医学杂志

<p><b>OBJECTIVE</b>To study the influence of thermochemotherapy on the activity of cytotoxic T lymphocyte (CTL) in peripheral blood of patients with oral maxillofacial cancer.</p><p><b>METHODS</b>Twenty-one subjects with oral maxillofacial cancer were treated by thermochemotherapy, and the activity of CTL in peripheral blood was analyzed.</p><p><b>RESULTS</b>Thermochemotherapy can obviously enhance the activity of CTL (P<0.01).</p><p><b>CONCLUSION</b>Thermochemotherapy can enhance the activity of CTL, thus enhance the patient's immune function. Therefore, it can enhance the antitumor response in whole body.</p>

Relationship between nuclear factor kappa B signal transduction way and the sensitivity of tongue carcinoma cell to Pingyangmycin chemotherapy / 华西口腔医学杂志

<p><b>OBJECTIVE</b>To examine the relationship between NF-kappaB signal transduction way and the sensitivity of Tca8113 carcinoma cell to Pingyangmycin chemotherapy.</p><p><b>METHODS</b>2 mg/L antisense p65 oligodeoxynucle-otides (5'-GAACAGTTCGTCCATGGCCG-3') was transfected into Tca8113 cells through Lepofectin vectin, then the cells were treated with 8 mg/L Pingyangmycin. After 3 h and 6 h, the expression of p65 in nucleus was studied with immunohistochemical methods and Western blot analyses. After 48 h, the inhibitory rate of cell growth was detected with MTT assay.</p><p><b>RESULTS</b>The NF-kappaB/p65 signal transduction way in Tca8113 cells was activated after treated with Pingyangmycin. But the transfection of antisense p65 oligodeoxynucle-otides inhibited the activation of the signal transduction, the p65 expression in nucleus was decreased significantly (P < 0.05) at 6 h, and the inhibitory rate of cell growth was increased significantly (P < 0.05) at 48 h.</p><p><b>CONCLUSION</b>The results suggested the chemotherapeutic sensitivity of Tca8113 carcinoma cell to Pingyangmycin was improved by the inhibition of NF-kappaB/p65 signal transduction.</p>

High expression of p65 transcription factor and its significance in tongue carcinoma / 华西口腔医学杂志

<p><b>OBJECTIVE</b>To examine the correlation between p65 expression and the metastasis and prognosis in tongue carcinoma.</p><p><b>METHODS</b>The immunohistochemical expression of p65 was examined in 50 cases of tongue carcinoma, and the clinical and histopathologic records were retrospectively analyzed.</p><p><b>RESULTS</b>Overexpression of p65 in cytoplasma and nucleus was found in 64% cases of tongue carcinoma. There was significant difference of p65 expression among different TNM stages, and the higher expression rate of p65 was found in higher stages. Overexpression of p65 was also related to lymph node metastasis. Significantly greater increase in expression levels of p65 was found in the metastatic cases (n = 22) compared to the nonmetastatic cases (n = 10). However, there was no significant difference of p65 expression among different histopathologic grading. The 5-year survival rate of p65 positive and negative group was 59.3% and 88.9% respectively, and there was significant difference in Kaplan-Meier survival curve (chi2 = 5.6, P < 0.05).</p><p><b>CONCLUSION</b>The results suggest that the expression of p65 was important to predict the metastasis and prognosis in tongue carcinoma.</p>

Effect of hypoxia on the expression of urokinase-type plasminogen activator in human tongue squamous cell carcinoma cells (Tca8113) / 华西口腔医学杂志

<p><b>OBJECTIVE</b>To observe the expression of urokinase-type plasminogen activator (uPA) in human tongue squamous carcinoma cells (Tca8113) under hypoxia, in order to explore the relation between hypoxia and invasion and metastasis of oral cancer.</p><p><b>METHODS</b>Under different hypoxic times(0, 3, 6, 12, 24 h), the expression of uPA protein was examined quantitatively using immunohistochemical technique (IH) and flow cytometry (FCM).</p><p><b>RESULTS</b>Hypoxia promoted the expression of uPA. The longer hypoxic time was, the higher expression of uPA was.</p><p><b>CONCLUSION</b>Hypoxia can promote invasion and metastasis of oral squamous carcinoma through stimulating the expression of uPA.</p>

Effect of microwave thermocoagulation on oral benign tumor / 华西口腔医学杂志

<p><b>OBJECTIVE</b>The purpose of this article was to observe the effect of by microwave thermocoagulation on oral benign tumor and to provide theoretical basis for clinic treatment of oral benign tumor.</p><p><b>METHODS</b>165 patients with oral benign tumor were included. There were 83 patients with oral bursa cyst, 42 patients with hemangioma, 19 patients with papilloma, 14 patients with fibrous hyperplasia and 7 patients with epulis. All the patients were treated by WB-100 microwave thermocoagulation.</p><p><b>RESULTS</b>144 patients were effective after the first treatment and 21 patients were effective after the second treatments. No side effect and reoccurrence were found after 3 years.</p><p><b>CONCLUSION</b>Microwave thermocoagulation is an effective and safe method in clinic treatment of oral benign tumor.</p>

Study on the expression level of Annexin I, cPLA(2) and PCNA in cleft-palate mice induced by dexamethasone / 中华口腔医学杂志

<p><b>OBJECTIVE</b>To test the changing expression level of Annexin I, cPLA(2) and PCNA in the palatine process of cleft-palate mice A/J and C57B/6j induced by dexamethasone. To discuss the developing mechanism of cleft-palate and the corresponding preventive methods.</p><p><b>METHOD</b>s Pregnant mice A/J and C57BL/6j were randomly divided into normal group as blank control, group with deformity induced by dexamethasone, group given VitB(12) as antagonist to deforming factor, group given only VitB(12). The relative quantity of Annexin I, Cpla2 and PCNA were detected by immunoblotting.</p><p><b>RESULTS</b>Among mice A/J, with the development of palatine process, Annexin I's expression level was increased in the normal group, and other groups showed the similar changes. Annexin I's expression level was significantly higher in group DEX and group DEX + VitB(12) than in normal group and VitB(12) group of, while there was no significant difference between normal group and VitB(12) group. But the changes of cPLA(2) and PCNA expression level was in an opposite direction, with development it decreased in the normal mice's palatine process. In mice C57B/6j there was no significant difference between normal group and group DEX on the measured quantity of Annexin I, cPLA(2) and</p><p><b>CONCLUSIONS</b>Annexin I and cPLA(2) introduce glucocorticoid to induce cleft-palate. VitB(12) can not inhibit DEX's enhancing effect on the expression level of Annexin I, but it can antagonize DEX's inhibiting effect on expression level of cPLA(2), which is probably one of the mechanisms why VitB(12) antagonize glucocorticoid's deforming effect.</p>