RESUMO
OBJECTIVES@#To identify the new designer drugs which are totally unknown and not in the routine testing list by the technologies such as high-resolution mass spectrometry in drug facilitated sexual assault, in order to solve the problem in actual cases.@*METHODS@#The milky fluid from an actual case was extracted and analyzed using LC-QE, ¹H-NMR and GC-MS, respectively. The accurate masses and cluster ions isotope patterns of unknown compound were obtained by LC-QE. The molecular formula was confirmed as C₁₆H₁₂C₂N₂O based on the protons number of ¹H-NMR. The isomers diclazepam and 4-chlorodiazepam were separated and detected with GC-MS.@*RESULTS@#The new designer benzodiazepine as diclazepam in the milky fluid was identified. The results provided direct evidence for the investigation and qualitative analysis of such cases.@*CONCLUSIONS@#The combined application of various methods, including LC-QE, ¹H-NMR and GC-MS, can be used to detect unknown new psychoactive substances.
Assuntos
Feminino , Humanos , Masculino , Benzodiazepinas/química , Benzodiazepinonas , Cromatografia Líquida/métodos , Drogas Desenhadas/química , Cromatografia Gasosa-Espectrometria de Massas/métodos , Espectrometria de Massas/métodos , Delitos Sexuais , Detecção do Abuso de Substâncias/métodos , Toxicologia/métodosRESUMO
Micellar liquid chromatography (MLC) is a reversed phase liquid chromatography with mobile phases containing surfactant above its critical micellar concentration (CMC). The basic mechanism and advantages of MLC in physicochemical analysis were reviewed, and its applications in analysis of drugs, barbiturates, benzodiazepines were chiefly introduced in this paper. MLC is a potential method to toxicological analysis due to strong selectivity, wide application scope and easy biological samples, etc.
Assuntos
Humanos , Analgésicos Opioides/análise , Barbitúricos/química , Benzodiazepinas/química , Cromatografia Líquida/métodos , Hipnóticos e Sedativos/química , Micelas , Reprodutibilidade dos Testes , Sensibilidade e Especificidade , Solventes/química , Tensoativos/químicaRESUMO
The reaction of 3-cyanoacctylindole I with trichloroacetonitrile afforded trichloroll1cthylpropen-1-one derivative 3 which upon the reaction with an excess of trichloroacetonitrilc yielded the ditrichlorome-thylpyrimidine derivative 5. It was converted to a variety of pyrazolo 7, dihydrazino 9. and isoxazolo pyrimidine II derivatives. The enaminonitrile 2 was used as a substrate to form the pyridine 13, 14, 16, diazepine 20 and pyranone 22 as well as 1, 2, 4-triazolo-25. 1, 2, 3, 4-tetrazolo-27, and benzimidazolo-pyrimidine 29 derivatives